1. Introduction
The Sper Scientific 300053 Pocket Digital Refractometer is a precision instrument designed for accurate measurement of Brix concentration in various solutions. This device offers laboratory-grade accuracy in a portable, easy-to-use format, suitable for field applications. It features automatic calibration, automatic temperature compensation (ATC), and an automatic shutoff function to conserve battery life.
Image 1: Sper Scientific 300053 Pocket Digital Refractometer, showing the device's front view with a digital display.
2. Product Overview and Features
The Sper Scientific 300053 is engineered for convenience and accuracy. Its compact design allows for easy portability, while its digital readout ensures precise measurements.
- Measurement Range: Brix 0 ~ 95%.
- Resolution: 0.1%.
- Accuracy: ±0.2%.
- Sample Volume: Requires samples as small as 1 ml.
- Display: Large LCD shows sample temperature and measurement results.
- Automatic Calibration: One-button calibration to distilled water.
- Automatic Temperature Compensation (ATC): Ensures accurate readings regardless of sample temperature fluctuations.
- Automatic Shutoff: Powers off after 3 minutes of inactivity to save battery.
- Durable Design: Sturdy, water-resistant case protects internal electronics.
- Battery Indicator: Alerts when battery replacement is needed (approximately 8000 readings per battery).
Image 2: The refractometer with its sample well cover open, revealing the prism and sample area.
3. Package Contents
Upon unpacking, verify that all items listed below are present:
- Sper Scientific 300053 Pocket Digital Refractometer
- Distilled Water (for calibration)
- Transfer Pipet
- AAA Battery
- Instruction Manual (this document)
- Carrying Case
Image 3: The refractometer shown with its included carrying case, distilled water vial, and transfer pipet.
4. Setup
- Battery Installation: Open the battery compartment located on the back of the device. Insert the AAA battery, ensuring correct polarity. Close the compartment securely.
- Initial Power On: Press the "READ" button to turn on the device. The display will illuminate.
- Clean the Prism: Before first use and before each measurement, ensure the prism surface is clean and dry. Use a soft, lint-free cloth to gently wipe the prism.
5. Calibration
For accurate measurements, the refractometer must be calibrated with distilled water. Perform calibration at room temperature (20-25°C / 68-77°F).
- Ensure the prism surface is clean and dry.
- Using the transfer pipet, place 2-3 drops of distilled water onto the prism surface. Ensure the water completely covers the prism.
- Close the prism cover gently.
- Press and hold the "ZERO" button for approximately 2 seconds until "CAL" appears on the display.
- Release the "ZERO" button. The display will show "END" indicating successful calibration.
- Wipe the distilled water from the prism and cover with a clean, dry cloth. The device is now ready for measurement.
6. Operation
Follow these steps to take a measurement:
- Ensure the refractometer has been calibrated as described in Section 5.
- Clean the prism surface thoroughly with a soft, lint-free cloth.
- Using the transfer pipet, place 2-3 drops of the sample liquid onto the prism surface. Ensure the sample completely covers the prism.
- Close the prism cover gently.
- Press the "READ" button. The display will first show the sample temperature, then the Brix percentage.
- Record the measurement.
- After measurement, immediately clean the prism and cover with distilled water and a soft, lint-free cloth to prevent residue buildup.
Image 4: The refractometer displaying a Brix reading of 25.8%, with the sample well open.
7. Maintenance
- Cleaning: Always clean the prism and cover immediately after use with distilled water and a soft, lint-free cloth. Do not use abrasive materials or harsh chemicals, as they can damage the prism surface.
- Storage: Store the refractometer in its carrying case in a cool, dry place, away from direct sunlight and extreme temperatures.
- Battery Replacement: When the low battery indicator appears, replace the AAA battery promptly to ensure continued accurate operation.
- Water Resistance: The device is water-resistant, but it is not submersible. Avoid prolonged exposure to water.
8. Troubleshooting
| Problem | Possible Cause | Solution |
|---|---|---|
| Inaccurate readings | Prism not clean; improper calibration; insufficient sample volume; sample temperature outside operating range. | Clean prism thoroughly; recalibrate with distilled water; ensure prism is fully covered by sample; allow sample to reach room temperature. |
| Display shows "CAL" repeatedly or fails to calibrate | Insufficient distilled water; prism not clean; "ZERO" button not held long enough. | Ensure 2-3 drops of distilled water cover the prism; clean prism; hold "ZERO" button for 2 seconds until "CAL" appears. |
| Device does not power on | Dead battery; incorrectly installed battery. | Replace AAA battery; check battery polarity. |
| Display is dim or flickering | Low battery. | Replace AAA battery. |
9. Specifications
| Parameter | Value |
|---|---|
| Model Number | 300053 |
| Measurement Range (Brix) | 0 ~ 95% |
| Resolution | 0.1% |
| Accuracy | ±0.2% |
| Automatic Temperature Compensation (ATC) | Yes |
| Minimum Sample Volume | 1 ml |
| Dimensions | 4.65 x 1.26 x 2.44 inches (118 x 32 x 62 mm) |
| Weight | 4.6 oz (130 g) |
| Power Source | AAA Battery |
| Battery Life | Approx. 8000 readings |
| Country of Origin | China |
Image 5: A table detailing specifications for different Sper Scientific refractometer models, including the 300053.
10. Warranty and Support
Sper Scientific is committed to providing reliable instruments and ensuring customer satisfaction. For warranty information, technical support, or service inquiries, please refer to the official Sper Scientific website or contact their customer service department. Keep your purchase receipt as proof of purchase.
For further assistance, you may visit the Sper Scientific Store on Amazon.
