Introduction
This manual provides essential information for the safe and efficient operation, installation, and maintenance of the Nuaire NU-5500 DH AutoFlow CO2 Air-Jacketed Incubator, Series 11. Please read this manual thoroughly before operating the incubator to ensure proper usage and to prevent damage or injury.
The Nuaire NU-5500 is designed to provide a stable and controlled environment for cell culture applications, maintaining precise temperature and CO2 levels.
Safety Information
Always follow standard laboratory safety procedures when operating this equipment. Ensure the incubator is placed on a stable, level surface. Do not block ventilation openings. Refer to local regulations for CO2 cylinder handling and storage.
- Ensure proper electrical grounding.
- Do not operate with damaged power cords.
- Use only specified CO2 gas of appropriate purity.
- Perform regular maintenance as described in this manual.
Setup
Unpacking and Inspection
Carefully remove the incubator from its packaging. Inspect for any visible damage that may have occurred during shipping. Report any damage to the carrier immediately.
Figure 1: Front view of the Nuaire NU-5500 DH AutoFlow CO2 Air-Jacketed Incubator, showing its overall design and an external thermometer unit placed on top.
Placement
Place the incubator on a sturdy, level surface capable of supporting its weight. Ensure adequate clearance around the unit for ventilation and maintenance access. Avoid direct sunlight or proximity to heat sources.
Electrical Connection
Connect the incubator to a grounded electrical outlet matching the specifications on the product label. The unit operates on 115 AC, 50/60Hz, 1 phase, 3 Amps.
Figure 2: Product label indicating the model number (NU-5500), series (11), serial number, year of manufacture (2004), and electrical rating (115 AC, 50/60Hz, 1 phase, 3 Amps).
CO2 Gas Connection
Connect the CO2 gas supply line to the appropriate inlet on the incubator. Ensure all connections are secure and leak-free. Use a medical or laboratory grade CO2 cylinder with a suitable pressure regulator.
Operating Instructions
Power On and Initial Warm-up
After connecting power and CO2, turn on the incubator. Allow the unit to stabilize for at least 24 hours before introducing cultures. The display will show current temperature and CO2 percentage.
Figure 3: Detailed view of the control panel, displaying the current temperature (37.2°C) and CO2 percentage (0.0%).
Setting Temperature and CO2 Levels
Use the SEL button and arrow keys (▲, ▼) on the control panel to navigate and adjust setpoints for temperature and CO2 concentration. Refer to the specific protocol for your cell culture requirements.
Figure 4: The control panel showing the temperature and CO2 displays, along with indicator lights for "HEAT JACKET", "INJECT CO2", "DOOR AJAR", "AIR CLASS ISO 5", "CO2 TANK 1", and "CO2 TANK 2". The "RUN SETUP" and "ALARM" indicators are also visible.
Loading Samples
Open the incubator door and place culture vessels on the shelves. Minimize door open time to maintain stable internal conditions. The interior features multiple shelves for sample placement.
Figure 5: Interior view of the incubator with the door open, revealing the stainless steel chamber and removable shelves designed for culture vessels.
Maintenance
Cleaning the Interior
Regularly clean the interior chamber with a mild, non-corrosive disinfectant. Ensure all surfaces are dry before closing the door. Remove shelves for thorough cleaning.
HEPA Filter Maintenance
The incubator is equipped with a HEPA filtration system. Check filters periodically as indicated on the unit. Replace filters according to manufacturer guidelines or when the indicator suggests.
Figure 6: Label indicating "HEPA FILTRATION SYSTEM" and "CHECK FILTERS PERIODICALLY", highlighting the importance of filter maintenance for maintaining air quality within the incubator.
Water Pan Refill
Ensure the water pan at the bottom of the chamber is filled with sterile distilled water to maintain humidity. Check water levels regularly and refill as needed.
Troubleshooting
| Problem | Possible Cause | Solution |
|---|---|---|
| Temperature Fluctuation | Door not sealed properly, ambient temperature changes, heater malfunction. | Check door seal, ensure stable room temperature, contact service if heater issue suspected. |
| CO2 Level Unstable | CO2 tank empty, gas line leak, sensor malfunction. | Replace CO2 tank, check all gas connections, calibrate or replace CO2 sensor. |
| Alarm Indicator On | Out-of-range temperature/CO2, door ajar. | Check setpoints, ensure door is fully closed, refer to specific alarm codes if available. |
Specifications
- Model: NU-5500
- Series: 11
- Type: CO2 Air-Jacketed Incubator
- Electrical Rating: 115 AC, 50/60Hz, 1 Phase, 3 Amps
- Manufacturer: Nuaire
- Year of Manufacture: 2004
- ASIN: B0DGRPWLMH
Warranty and Support
For warranty information and technical support, please refer to the original purchase documentation or contact the vendor. This manual covers the operational aspects of the Nuaire NU-5500 DH AutoFlow CO2 Air-Jacketed Incubator, Series 11.
For parts and service inquiries, please provide the model number (NU-5500) and serial number (e.g., 94249102504 from the product label) of your unit.
