1. Introduction
This manual provides comprehensive instructions for the proper use, setup, operation, and maintenance of the Colombo Aquatest NO2 Nitrite Pond Test Kit. This kit is designed for accurately measuring nitrite concentration in pond water, ensuring optimal water quality for aquatic life. Each kit contains sufficient reagents for approximately 40 tests.

Image: The retail packaging of the Colombo Aquatest NO2 Nitrite Pond Test Kit. The box is primarily yellow and black, featuring illustrations of various aquatic life such as koi, sturgeon, and small fish. Text on the box indicates 'Aquatest NO2 test Nitrite' and mentions '40x tests' for pond, fresh, and sea water.
2. Safety Information
WARNING:
Please read all product packaging literature and product labels carefully before use. Keep out of reach of children and pets. Avoid contact with skin and eyes. In case of contact, rinse immediately with plenty of water. Do not ingest. If swallowed, seek immediate medical attention. Dispose of used reagents and samples responsibly according to local regulations.
3. Kit Components and Setup
Before beginning, ensure all components are present and undamaged. The kit typically includes:
- Nitrite Reagent 1 (Liquid)
- Nitrite Reagent 2 (Liquid)
- Test Tube(s) with cap
- Color Chart for result comparison
- Instruction Leaflet (this manual serves as a detailed guide)
3.1 Initial Setup
- Unpack all components from the kit box.
- Familiarize yourself with the reagents and the color chart.
- Ensure the test tube(s) are clean and dry before first use.
4. Operating Instructions: Performing the Nitrite Test
Follow these steps carefully to ensure accurate nitrite measurements:
- Prepare the Sample: Fill the test tube with 5 ml of the pond water to be tested. Ensure the water sample is taken from a representative area of the pond, away from surface film or debris.
- Add Reagent 1: Add 5 drops of Nitrite Reagent 1 to the test tube. Cap the test tube and gently invert it several times to mix the solution.
- Add Reagent 2: Add 5 drops of Nitrite Reagent 2 to the test tube. Cap the test tube again and gently invert it several times to mix thoroughly.
- Wait for Reaction: Allow the test tube to stand undisturbed for 5 minutes. During this time, the color of the solution will develop.
- Compare Results: After 5 minutes, compare the color of the solution in the test tube with the color chart provided in the kit. Hold the test tube against the white background of the color chart in good lighting conditions (preferably natural daylight).
- Interpret Reading: Match the color in the test tube to the closest color on the chart. The corresponding number indicates the nitrite concentration in mg/L (ppm).
Note: For best accuracy, perform tests at consistent times of day and under similar lighting conditions. Rinse the test tube thoroughly with distilled or deionized water immediately after each use.
5. Maintenance
Proper maintenance of your test kit components will ensure longevity and accuracy:
- Cleaning Test Tubes: After each test, thoroughly rinse the test tube(s) multiple times with clean tap water, then with distilled or deionized water to remove any residual chemicals. Allow them to air dry completely before storing.
- Reagent Storage: Store reagents in their original bottles, tightly capped, in a cool, dark place away from direct sunlight and extreme temperatures. Do not refrigerate unless specifically instructed.
- Expiry Dates: Check the expiry dates on the reagent bottles. Expired reagents may yield inaccurate results and should be replaced.
- Kit Storage: Store the entire kit in its original packaging in a dry, safe place to protect components from damage and contamination.
6. Troubleshooting
If you encounter issues while using the test kit, consider the following:
| Problem | Possible Cause | Solution |
|---|---|---|
| Inaccurate or inconsistent readings |
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| Color does not develop or is faint |
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| Difficulty matching color on chart |
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7. Specifications
| Product Name | Colombo Aquatest NO2 Nitrite Pond Test Kit |
| Brand | Colombo |
| Model Number (ASIN) | B07T4JSH56 |
| Measurement Parameter | Nitrite (NO2) |
| Test Capacity | Approximately 40 tests |
| Care Instructions | Disposable (referring to the test itself) |
| Date First Available | June 14, 2019 |
| Manufacturer | Colombo |
8. Warranty and Support
For information regarding product warranty, returns, or technical support, please refer to the official Colombo website or contact their customer service directly. Keep your purchase receipt as proof of purchase.
For general inquiries or further assistance, you may also contact the retailer from whom you purchased this product.





