1. Introduction
Welcome to the instruction manual for your Atago Master-500 Refractometer. This precision instrument is designed for accurate measurement of Brix values in a wide range of liquids. It features a full measurement range from 0 to 90% Brix, a robust heat-resistant design suitable for high-temperature samples, and IP65 water resistance for enhanced durability and easy cleaning.
2. Safety Information
- Handle the refractometer with care to prevent damage to the optical components.
- Do not immerse the device in water beyond its IP65 rating. IP65 protects against low-pressure water jets, not full immersion.
- Keep the device out of reach of children.
- Avoid exposing the refractometer to extreme temperatures or direct sunlight for prolonged periods.
- Use only non-abrasive materials for cleaning the prism and daylight plate.
3. Package Contents
Please verify that all items are present and undamaged upon unpacking:
- Atago Master-500 Refractometer
- Instruction Manual
- Protective Case (if included)
- Cleaning Cloth (if included)
4. Product Features
- Scale Range: Brix 0.0~90.0%
- Minimum Scale: Brix 1.0%
- Heat Resistance: Designed for measurements of high-temperature liquids.
- Water Resistance: IP65 rated for protection against dust and water jets.
- Origin: Manufactured in Japan.

Image of the Atago Master-500 Refractometer, a handheld device with a prism and eyepiece for Brix measurement.
5. Setup
- Unpack: Carefully remove the refractometer from its packaging and inspect it for any signs of damage.
- Clean Prism: Ensure the prism surface and daylight plate are clean and dry before first use. Use a soft, lint-free cloth.
- Calibration (Optional): For initial verification or if accuracy is in doubt, calibrate the device. For 0% Brix, place a few drops of distilled water on the prism, close the daylight plate, and check the reading. Adjust if necessary according to specific calibration instructions for your model (refer to detailed instructions if a calibration screw is present).
6. Operating Instructions
- Prepare Sample: Ensure your liquid sample is well-mixed and free of air bubbles.
- Apply Sample: Open the daylight plate. Place 2-3 drops of the sample liquid onto the main prism surface. Ensure the prism is fully covered.
- Close Plate: Gently close the daylight plate. This will spread the sample evenly across the prism.
- View Reading: Hold the refractometer towards a natural light source or a bright lamp. Look through the eyepiece.
- Focus: If the scale is blurry, rotate the eyepiece to bring the scale into sharp focus.
- Read Value: The Brix value is indicated by the boundary line between the upper blue field and the lower clear field. Read the value where this line intersects the scale.
- Clean After Use: Immediately after each measurement, open the daylight plate and clean the prism and daylight plate thoroughly with a soft, damp cloth. Dry completely.
7. Maintenance
- Cleaning: Always clean the prism and daylight plate immediately after use to prevent sample residue from drying and affecting future readings. Use a soft, lint-free cloth moistened with water.
- Storage: Store the refractometer in its protective case in a cool, dry place, away from direct sunlight and extreme temperatures.
- Avoid Impact: Do not drop the device or subject it to strong impacts, as this can damage the internal optics.
- No Solvents: Do not use abrasive cleaners, solvents, or harsh chemicals on any part of the refractometer.
- Inspection: Periodically inspect the device for any signs of damage or wear.
8. Troubleshooting
| Problem | Possible Cause | Solution |
|---|---|---|
| Inaccurate readings | Dirty prism; improper calibration; insufficient sample; temperature difference. | Clean prism thoroughly; recalibrate if necessary; ensure adequate sample volume; allow sample to reach ambient temperature. |
| Blurred field of view | Eyepiece out of focus. | Rotate the eyepiece to adjust focus until the scale is clear. |
| Boundary line not visible | Insufficient light; no sample; sample too dark or opaque. | Ensure adequate light source; apply sample; dilute very dark samples if appropriate for measurement. |
| Water inside the device | Exceeded IP65 water resistance limits (e.g., full immersion). | Discontinue use immediately. Contact customer support for repair or replacement. |
9. Specifications
- Model: Master-500
- Part Number: 2363
- Internal Model Number: 1197V71EA
- Scale Range: Brix 0.0~90.0%
- Minimum Scale: Brix 1.0%
- Heat Resistance: Yes
- Water Resistance: IP65
- Weight: Approximately 285 g
- Dimensions: Approximately 19.8 x 6.9 x 6.2 cm
- Manufacturer: Atago
- Country of Origin: Japan
10. Warranty and Support
For detailed warranty information, technical assistance, or service inquiries regarding your Atago Master-500 Refractometer, please contact your authorized Atago distributor or visit the official Atago website. Please have your model and part number available when contacting support.