1. Introduction
This manual provides essential instructions for the safe and efficient operation of the Implen C40 NanoPhotometer UV/Vis Spectrophotometer Mobile System. The C40 NanoPhotometer is designed for reliable protein and DNA measurements in both mobile and laboratory environments. It features microvolume measurement capabilities, requiring only 0.3 µl of sample, and offers a low limit of detection for various biomolecules. Its simplified optics ensure stable performance without the need for recalibration.
The system supports flexible control and data collection via various devices and includes multiple interface connections for data transfer. This model, the C40-Mobile, is equipped with a built-in touchscreen tablet for direct interaction.
2. Product Overview

Figure 1: Implen C40 NanoPhotometer UV/Vis Spectrophotometer Mobile System. The device is white and black, with a black touchscreen display angled upwards from the top surface, showing various application icons.
Key Features:
- Microvolume measurement: Requires only 0.3 µl sample size.
- High sensitivity: Limit of detection down to 0.1 ng/µl (DS DNA) or 0.003 mg/µl (BSA).
- Stable optics: Two fixed and sealed pathlengths prevent drift and eliminate recalibration needs.
- Connectivity: Equipped with Wi-Fi, USB a/b, HDMI, and LAN interfaces.
- Data transfer: Front USB Flash drive port for quick data transfer.
- Flexible control: Compatible with computer, Mac, smartphone, or tablet.
- Integrated touchscreen: C40-Mobile model includes a built-in touchscreen tablet.
- Security: Slot for a Kensington lock provides anti-theft security.
- Power: Operates on 110/220V with a built-in Li-ion battery (up to 8-hour life).
- Detector: 3648 CCD array.
- Bandwidth: < 1.8 nm.
3. Setup and Initial Use
3.1 Unpacking and Inspection
- Carefully remove the NanoPhotometer from its packaging.
- Inspect the device for any signs of damage during transit. If damage is found, contact your supplier immediately.
- Ensure all accessories listed in the packing slip are present.
3.2 Power Connection and Charging
- Connect the provided power adapter to the NanoPhotometer and a suitable 110/220V AC power outlet.
- The internal Li-ion battery will begin charging automatically. A full charge can provide up to 8 hours of mobile operation.
- It is recommended to fully charge the device before its first mobile use.
3.3 Connectivity Options
- Wi-Fi: Access the device settings via the touchscreen to connect to a local Wi-Fi network for wireless control and data transfer.
- USB: Use the USB a/b ports to connect to a computer or for direct data transfer via a USB flash drive.
- HDMI: Connect an external display using the HDMI port for larger viewing.
- LAN: For wired network connectivity, use the LAN port.
3.4 Security
The NanoPhotometer includes a slot for a Kensington lock. This can be used to secure the device to a workstation, preventing unauthorized removal.
4. Operating Instructions
4.1 Powering On and User Interface
- Press the power button to turn on the device. The integrated touchscreen will display the main application interface.
- Navigate through the applications using the touchscreen. Applications are typically categorized for Nucleic Acid, Protein UV, Protein A280, Standard Methods, and Kinetics measurements.
4.2 Sample Measurement
- Prepare Sample: Ensure your sample volume is at least 0.3 µl.
- Apply Sample: Carefully pipette the sample onto the designated measurement area. The simplified optics with fixed pathlengths ensure consistent results.
- Select Application: On the touchscreen, select the appropriate measurement application (e.g., Nucleic Acid, Protein UV).
- Initiate Measurement: Follow the on-screen prompts to start the measurement. The device will automatically perform the analysis.
- Review Results: Measurement results will be displayed on the screen.
4.3 Data Management
- Saving Data: Results can be saved directly to the device's internal memory.
- Exporting Data:
- USB Flash Drive: Insert a USB flash drive into the front port and use the on-screen options to transfer data.
- Network Transfer: Utilize Wi-Fi or LAN connections to transfer data to a connected computer, Mac, smartphone, or tablet.
5. Maintenance
5.1 Cleaning
- After each measurement, gently wipe the sample measurement area with a lint-free cloth moistened with deionized water or a mild cleaning solution.
- Ensure no liquid enters the internal components of the device.
- Clean the exterior of the device with a soft, damp cloth. Avoid abrasive cleaners or solvents.
5.2 Storage
When not in use, store the NanoPhotometer in a clean, dry environment, away from direct sunlight and extreme temperatures.
5.3 Battery Care
To prolong battery life, avoid completely discharging the battery frequently. Recharge the device regularly, especially if it will be stored for an extended period.
6. Troubleshooting
| Problem | Possible Cause | Solution |
|---|---|---|
| Device does not power on. | No power, discharged battery, or power adapter issue. | Ensure power adapter is securely connected and plugged into a live outlet. Allow battery to charge for at least 30 minutes before attempting to power on again. |
| Measurement results are inconsistent. | Improper sample application, dirty measurement area, or air bubbles in sample. | Ensure sample is applied correctly and free of air bubbles. Clean the measurement area thoroughly. Use appropriate sample volume. |
| Cannot connect to Wi-Fi/Network. | Incorrect network settings, out of range, or network issue. | Verify Wi-Fi password and network settings. Ensure the device is within range of the Wi-Fi router. Check network status. |
| Data transfer to USB drive fails. | USB drive not properly inserted, incompatible format, or full. | Ensure USB drive is fully inserted. Check if the USB drive is formatted correctly (e.g., FAT32). Verify sufficient space on the drive. |
7. Technical Specifications
| Feature | Detail |
|---|---|
| Model | C40-Mobile |
| Brand | Implen |
| Manufacturer | Cole-Parmer |
| Bandwidth | < 1.8 nm |
| Battery | Built-in Li-ion, up to 8-hour life max |
| Detector | 3648 CCD array |
| Power (Hz) | 50/60 |
| Sample Volume | 0.3 µl (minimum) |
| Limit of Detection (DS DNA) | 0.1 ng/µl |
| Limit of Detection (BSA) | 0.003 mg/µl |
| Connectivity | Wi-Fi, USB a/b, HDMI, LAN |
| Package Dimensions | 17 x 12 x 11 inches; 16 Pounds |
| ASIN | B01J13HNB0 |
| Date First Available | March 14, 2017 |
8. Warranty and Support
8.1 Warranty Information
Specific warranty terms and conditions for the Implen C40 NanoPhotometer are provided by the manufacturer, Implen, or your authorized vendor at the time of purchase. Please refer to your purchase documentation or contact your vendor for detailed warranty information.
8.2 Technical Support
For technical assistance, troubleshooting beyond this manual, or service requests, please contact your authorized Implen distributor or the manufacturer directly. Have your model number (C40-Mobile) and ASIN (B01J13HNB0) ready when contacting support.





