1. Introduction
This manual provides instructions for the proper use and maintenance of the Labnet International E0164 Large Comb Set. This set includes two combs designed for creating wells in electrophoresis gels, featuring configurations of 14 and 28 teeth. These combs are essential accessories for various molecular biology and biochemistry applications requiring gel electrophoresis.
Figure 1: Components of the Labnet International E0164 Large Comb Set. The image displays the two comb units, a gel casting tray, and an associated electrophoresis unit, illustrating the complete setup for gel preparation.
2. Setup and Preparation
Proper setup ensures optimal performance and well formation.
- Inspect Components: Before each use, visually inspect both combs for any signs of damage, such as bent teeth or cracks. Ensure they are clean and free from debris.
- Select Comb: Choose the appropriate comb (14-tooth or 28-tooth) based on the desired number of wells for your experiment.
- Insert Comb into Casting Tray: Carefully insert the selected comb into the designated slots of your gel casting tray. Ensure the comb is fully seated and perpendicular to the tray surface. The teeth should be positioned to create wells of the desired depth in the gel.
- Prepare Gel Solution: Prepare your agarose or polyacrylamide gel solution according to your experimental protocol.
3. Operating Instructions
Follow these steps for casting gels with the Labnet comb set:
- Pour Gel: Once the comb is securely in place within the casting tray, carefully pour the prepared gel solution into the tray. Pour slowly to avoid air bubbles around the comb teeth.
- Allow Gel to Polymerize: Let the gel polymerize completely. The polymerization time will vary depending on the type and concentration of the gel. Do not disturb the setup during this process.
- Remove Comb: After the gel has fully polymerized, gently and slowly pull the comb straight upwards from the gel. Avoid twisting or sudden movements, which can damage the wells.
- Transfer Gel: Carefully transfer the gel with its newly formed wells to the electrophoresis unit for sample loading and running.
4. Maintenance and Care
Proper maintenance extends the lifespan of your comb set.
- Cleaning: Immediately after use, rinse the combs thoroughly with deionized water to remove any residual gel or buffer.
- Disinfection: If necessary, the combs can be disinfected using standard laboratory disinfectants compatible with plastic materials. Rinse thoroughly after disinfection.
- Storage: Store the combs in a clean, dry place, preferably in their original packaging or a protective container, to prevent damage to the teeth.
- Avoid Harsh Chemicals: Do not use abrasive cleaners or strong organic solvents that may degrade the plastic material.
5. Troubleshooting
| Problem | Possible Cause | Solution |
|---|---|---|
| Uneven or distorted wells | Comb not inserted straight; gel poured too quickly; comb removed too fast or at an angle. | Ensure comb is inserted perpendicularly. Pour gel slowly. Remove comb gently and straight upwards. |
| Damaged comb teeth | Improper handling; forceful removal from gel; contact with sharp objects. | Handle combs with care. Always remove gently. Store in a protective manner. Replace damaged combs. |
| Residual gel in wells | Incomplete polymerization; comb removed too early. | Allow sufficient time for gel polymerization before comb removal. |
6. Specifications
- Brand: Labnet International
- Model Number: 1196M16PK (E0164)
- Comb Configuration: 14 teeth and 28 teeth (included in set)
- Package Dimensions: 10 x 10 x 10 inches
- Package Weight: 11.5 Pounds
- Manufacturer: Labnet International, Inc.
- First Available: June 25, 2016