1. Product Overview
The YMC America OS12S03-1503WT Pro C8 column is designed for high-performance liquid chromatography (HPLC) applications, specifically optimized for the separation of basic and metal chelating compounds. This column features a robust packing material with precise specifications to ensure reliable and reproducible results in analytical and preparative chromatography.
- Column Type: Pro C8, suitable for basic and metal chelating compounds.
- Particle Size: 3 micrometers.
- Pore Size: 120 Angstrom.
- Dimensions: 3mm Internal Diameter (ID) and 150mm Length.
- Origin: Manufactured in the United States.
Figure 1: Illustration of a YMC America Pro C8 Chromatography Column. This image displays the typical appearance of a chromatography column, which is a cylindrical metal tube with end fittings, used in analytical chemistry for separating chemical components.
2. Specifications
| Attribute | Value |
|---|---|
| Model Number | OS12S03-1503WT |
| Column Type | Pro C8 |
| Particle Size | 3 µm |
| Pore Size | 120 Å |
| Internal Diameter (ID) | 3 mm |
| Length | 150 mm |
| Manufacturer | Thomas Scientific (for distribution) |
| ASIN | B00UDN8YSO |
3. Setup
Proper setup is crucial for optimal performance and longevity of your chromatography column. Follow these guidelines:
- Inspection: Upon receipt, carefully inspect the column for any signs of damage during shipping. Verify that the model number matches your order.
- Storage: Store the column in its original packaging at room temperature, away from direct sunlight or extreme temperatures, until ready for use. Ensure end caps are securely in place.
- System Compatibility: Ensure your HPLC system is compatible with the column dimensions (3mm ID x 150mm length) and pressure limits.
- Installation:
- Connect the column to the HPLC system using appropriate low-dead-volume fittings. Ensure connections are finger-tight, then tighten an additional quarter to half turn with a wrench to prevent leaks.
- Note the flow direction arrow on the column label and install it accordingly.
- Equilibration: Before first use, equilibrate the column with your mobile phase.
- Flush the column with at least 10-20 column volumes of the initial mobile phase at a low flow rate (e.g., 0.1-0.2 mL/min) to remove any storage solvent and stabilize the stationary phase.
- Increase the flow rate gradually to the desired operating flow rate.
- Monitor the baseline stability with your detector. Equilibration is complete when a stable baseline is achieved.
Note: Always refer to your HPLC system's manual for specific connection and operation procedures.
4. Operating Instructions
Adhere to these guidelines for effective operation of the Pro C8 column:
- Mobile Phase Preparation:
- Use high-purity solvents and reagents.
- Filter all mobile phases through a 0.2 µm or 0.45 µm membrane filter to remove particulates.
- Degas mobile phases thoroughly to prevent air bubbles in the system.
- Sample Preparation:
- Filter all samples through a 0.2 µm or 0.45 µm syringe filter to protect the column from particulate matter.
- Ensure samples are dissolved in a solvent compatible with the mobile phase to avoid precipitation upon injection.
- Flow Rate and Pressure:
- Operate within the recommended flow rate range for a 3mm ID column (typically 0.3-0.8 mL/min, depending on particle size and application).
- Monitor system pressure. Sudden increases in pressure may indicate column clogging or blockages.
- Temperature:
- Maintain a stable column temperature for reproducible results. Use a column oven if available.
- Avoid operating at excessively high temperatures, which can degrade the stationary phase.
- Shutdown:
- Before shutting down the system or storing the column, flush it with a suitable storage solvent (e.g., 100% acetonitrile or methanol) to remove salts and buffers.
- Never leave the column in an aqueous buffer solution for extended periods.
5. Maintenance
Regular maintenance extends the lifespan and ensures consistent performance of your Pro C8 column:
- Cleaning:
- If column performance degrades (e.g., peak broadening, tailing, or increased backpressure), a cleaning procedure may be necessary.
- Consult YMC America's technical support or product literature for specific cleaning protocols for C8 columns. Generally, strong organic solvents or specific wash solutions are used.
- Storage:
- For short-term storage (days to weeks), flush the column with 100% organic solvent (e.g., acetonitrile or methanol) and cap the ends securely.
- For long-term storage (months), store in 100% organic solvent, cap securely, and keep in a cool, dark place.
- Never store the column dry or in an aqueous buffer.
- Guard Columns: Consider using a compatible guard column to protect the analytical column from irreversible adsorption of sample components and particulate matter, significantly extending its lifespan.
6. Troubleshooting
Common issues and potential solutions for chromatography columns:
| Problem | Possible Cause | Solution |
|---|---|---|
| High Backpressure |
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| Low Backpressure |
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| Peak Tailing/Broadening |
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| Retention Time Shifts |
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For further assistance, contact YMC America technical support.
7. Warranty and Support
YMC America products are manufactured to high-quality standards. For specific warranty information, please refer to the documentation included with your purchase or visit the official YMC America website. In case of technical issues, questions, or support needs, please contact YMC America customer service or technical support directly.
Contact Information: Please refer to the YMC America official website for the most current contact details.
