Hach Chlorine (Total) Test Kit Instruction Manual

1. Product Overview

The Hach 225401 Chlorine (Total) Test Kit, Model CN-65, is designed for the accurate measurement of total chlorine levels in various water samples. Chlorine is a critical disinfectant in drinking water, swimming pools, cooling towers, and wastewater treatment. This kit provides a reliable method for monitoring chlorine, which is essential for both safety and economic considerations.

This kit primarily utilizes the drop count titration method, a straightforward and cost-effective analytical technique. It includes all necessary reagents and apparatus, conveniently housed in a durable carrying case, making it suitable for field analysis.

An image showing the Hach 225401 Chlorine (Total) Test Kit, Model CN-65. It includes a light blue carrying case, a dark bottle with a dropper, two clear plastic bags containing reagent packets, a 50 mL glass flask, a clear glass test tube, and a small clear glass bottle.

2. Kit Components and Setup

Before beginning any tests, ensure all components of the kit are present and in good condition. The kit is designed for ease of use in various environments.

2.1. Included Components:

• Rugged carrying case
• Reagents for total chlorine testing (e.g., DPD colorimetric reagents, thiosulfate)
• Apparatus for analysis (e.g., titration vessel, measuring devices)

2.2. Initial Setup:

No complex assembly is required. Simply open the carrying case and familiarize yourself with the contents. Ensure all reagent bottles are securely capped and reagent packets are sealed until ready for use. Store the kit in a cool, dry place away from direct sunlight.

3. Operating Instructions

The Hach 225401 kit utilizes the drop count titration method for determining total chlorine. This method involves adding a titrant drop by drop to a sample until a color change indicates the endpoint of the reaction.

3.1. General Procedure for Total Chlorine Measurement:

1. Prepare the Sample: Collect a representative water sample in a clean sample bottle or flask provided with the kit. The volume required will be specified in the detailed instructions accompanying the reagents.
2. Add Reagents: Follow the specific instructions on the reagent packets or bottles for the correct sequence and amount of reagents to add to the sample. Typically, DPD reagents are added first to develop a color proportional to the chlorine concentration.
3. Perform Titration: Using the dropper bottle, add the titrant solution (e.g., Sodium Thiosulfate) drop by drop to the prepared sample. Swirl or mix the sample gently after each drop.
4. Observe Color Change: Continue adding drops until a distinct and permanent color change occurs, indicating the titration endpoint. For total chlorine, this often involves a color disappearing or changing to clear.
5. Count Drops and Calculate: Record the exact number of drops of titrant used. Refer to the conversion chart or formula provided with the kit to calculate the total chlorine concentration in mg/L (ppm).
6. Record Results: Document your findings accurately for record-keeping and compliance.

Note: Always refer to the specific instructions provided with the reagent bottles and packets for precise measurement procedures, as these may vary slightly depending on the specific test range and reagents.

4. Care and Maintenance

Proper care and maintenance will ensure the longevity and accuracy of your Hach test kit.

• Clean Apparatus: After each use, thoroughly rinse all glassware and plasticware with distilled or deionized water. Allow them to air dry completely before storing.
• Store Reagents Properly: Keep all reagents tightly capped and stored in their original containers within the kit's carrying case. Store the kit in a cool, dark, and dry place, away from extreme temperatures or direct sunlight, to preserve reagent integrity.
• Check Expiration Dates: Periodically check the expiration dates on all reagents. Expired reagents can lead to inaccurate results and should be replaced.
• Handle with Care: Avoid dropping or subjecting the kit to harsh impacts, which could damage the components or carrying case.

5. Troubleshooting

If you encounter issues during testing, consider the following common troubleshooting tips:

• Inaccurate Results: Ensure reagents are not expired, glassware is clean, and the correct number of drops is counted accurately. Verify that the sample is representative and free from excessive turbidity or interfering substances.
• No Color Change: Check if reagents were added in the correct order and quantity. Ensure the sample actually contains chlorine.
• Difficulty with Endpoint: Ensure adequate lighting for observing the color change. Practice the titration technique to become familiar with the endpoint.
• Damaged Components: Inspect all kit components for cracks, leaks, or damage. Replace any compromised items immediately.

For persistent issues, refer to the detailed troubleshooting guide in the complete product manual or contact Hach customer support.

6. Product Specifications

7. Warranty and Support

For information regarding product warranty, technical support, or replacement parts, please contact Hach customer service directly. Contact details can typically be found on the Hach official website or included in the full product documentation.

When contacting support, please have your product model number (CN-65) and item number (225401) readily available.