1. Product Overview
The YSI 251401Y DPD Free Chlorine Reagent Powder is an EPA-approved chemical reagent designed for accurate measurement of free chlorine concentrations. This product is specifically formulated for use with YSI pHotoFlex and 900 Chlorine Colorimeter instruments, providing reliable results within a range of 0.02 to 2 mg/L. Each pack contains 100 individual 10ml volume powder packets, ensuring convenience and consistent dosing for laboratory and field applications.
Image 1.1: A single packet of YSI 251401Y DPD Free Chlorine Reagent Powder. The label indicates "Powder Packets," "Part Number: 251401Y," "Lot Code: L 01 A," and "Exp: 01.2017." It also includes safety warnings and manufacturer contact information.
2. Safety Information and Handling
This product is intended for professional laboratory and field use only. Adherence to all safety guidelines is critical for proper and safe operation.
- Use as Directed: This product is to be used only as directed. Any other use is prohibited.
- Keep Away from Children: Store and use this product out of reach of children.
- Safety Data Sheet (SDS): A detailed Safety Data Sheet is available upon request for professional users. It contains comprehensive information regarding chemical properties, hazards, safe handling, emergency procedures, and disposal.
- Personal Protective Equipment (PPE): Always wear appropriate personal protective equipment, such as safety glasses and gloves, when handling chemical reagents.
- Ventilation: Use in a well-ventilated area.
- Disposal: Dispose of used reagents and samples according to local environmental regulations.
3. Setup and Preparation
Before beginning any measurement, ensure you have the necessary equipment and that your colorimeter is calibrated according to its specific user manual.
3.1 Required Materials (Not Included)
- YSI pHotoFlex or 900 Chlorine Colorimeter
- Clean sample cells/vials
- Deionized water (for rinsing and blanking)
- Sample to be tested
3.2 Reagent Preparation
The YSI 251401Y DPD Free Chlorine Reagent is provided in pre-measured powder packets, eliminating the need for manual weighing or dilution. Each packet contains the precise amount of reagent for a 10ml sample volume.
4. Operating Instructions for Free Chlorine Measurement
Follow these steps for accurate free chlorine measurement using the YSI 251401Y DPD Free Chlorine Reagent Powder with a compatible YSI colorimeter.
- Prepare Sample Cell: Rinse a clean sample cell thoroughly with deionized water, then with a small amount of the sample to be tested.
- Fill Sample Cell: Fill the sample cell with 10 ml of the water sample to be analyzed. Ensure the sample volume is precise.
- Add Reagent: Carefully open one YSI 251401Y DPD Free Chlorine Reagent Powder packet. Add the entire contents of the powder packet to the 10 ml sample in the cell.
- Mix: Cap the sample cell and invert it gently several times to dissolve the powder completely. Avoid shaking vigorously, which can introduce air bubbles. A pink color will develop if free chlorine is present.
- Reaction Time: Allow a reaction time of approximately 1 minute for the color to fully develop. Consult your colorimeter's manual for specific reaction time recommendations.
- Measure: Insert the prepared sample cell into the YSI pHotoFlex or 900 Chlorine Colorimeter. Follow the instrument's instructions to initiate the measurement. The colorimeter will display the free chlorine concentration in mg/L.
- Record Results: Record the measurement results immediately.
- Clean Up: After measurement, empty the sample cell and rinse it thoroughly with deionized water.
Note: For best results, perform measurements promptly after sample collection. The DPD method is sensitive to light and temperature; protect samples and reagents from direct sunlight and extreme temperatures.
5. Maintenance and Storage
Proper storage of the reagent packets is essential to maintain their integrity and ensure accurate results.
- Storage Conditions: Store the YSI 251401Y DPD Free Chlorine Reagent Powder packets in a cool, dry place, away from direct sunlight and extreme temperatures.
- Original Packaging: Keep the packets in their original packaging until ready for use to protect them from moisture and contamination.
- Expiration Date: Always check the expiration date printed on each packet (e.g., "Exp: 01.2017" as shown in Image 1.1). Do not use expired reagents, as they may yield inaccurate results.
- Disposal: Dispose of expired or unused reagents according to local hazardous waste regulations.
6. Troubleshooting
This section addresses common issues that may arise during the use of the DPD Free Chlorine Reagent.
| Problem | Possible Cause | Solution |
|---|---|---|
| No color development or faint color when chlorine is expected. |
|
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| Inconsistent or erratic readings. |
|
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7. Specifications
- Product Name: YSI 251401Y DPD Free Chlorine Reagent Powder
- Part Number: 251401Y
- Application: Free Chlorine Measurement
- Compatibility: YSI pHotoFlex and 900 Chlorine Colorimeter
- Approval: EPA Approved
- Volume per Packet: 10 ml (for 10 ml sample)
- Measurement Range: 0.02 to 2 mg/L
- Quantity: Pack of 100 packets
- Item Form: Powder
- Item Weight: Approximately 2.4 Ounces (for the pack)
8. Warranty and Support
For technical support, product inquiries, or to request a Safety Data Sheet (SDS), please contact YSI directly using the information below.
- Manufacturer: YSI
- Address: 1725 Brannum Lane, Yellow Springs, OH 45387, USA
- Telephone: 800-897-4151; 1-937-767-7241
- Website: www.ysi.com
- Email: environmental@ysi.com
YSI stands behind the quality of its products. Please refer to the official YSI website or contact customer support for detailed warranty information applicable to this reagent.
