Introduction
This manual provides essential instructions for the proper use, handling, and storage of the Sper Scientific 300007 Brix Calibration Solution 28%. This solution is designed for calibrating refractometers and other instruments that measure Brix levels, ensuring accurate readings for various applications.
Product Overview
The Sper Scientific 300007 is a precisely formulated 28% Brix calibration solution. It comes in a convenient bottle, suitable for single or multiple uses depending on the application. Accurate calibration is crucial for reliable measurement results.

Image 1: A small, white plastic bottle with a ribbed white cap. The label on the bottle reads "300007 Brix Calibration Sol SPER SCIENTIFIC". This image depicts the packaging of the 28% Brix calibration solution.
Setup
- Preparation: Ensure your refractometer or measuring instrument is clean and dry before calibration. Refer to your instrument's specific cleaning instructions.
- Temperature Stabilization: Allow the calibration solution and the instrument to reach a stable ambient temperature, ideally between 20°C to 25°C (68°F to 77°F). Significant temperature variations can affect measurement accuracy.
- Opening the Bottle: Carefully uncap the solution bottle. Avoid contaminating the solution with foreign substances.
Operating Procedures
Follow these steps to calibrate your instrument using the Sper Scientific 300007 Brix Calibration Solution:
- Apply Solution: Place a few drops of the 28% Brix calibration solution onto the prism of your refractometer or the sample well of your instrument. Ensure the prism or sample well is completely covered without air bubbles.
- Wait for Stabilization: Allow a short period (e.g., 30-60 seconds) for the solution to stabilize on the instrument's surface and for its temperature to equalize with the instrument.
- Take Reading: Read the Brix value displayed by your instrument. For a refractometer, look through the eyepiece and note the reading at the boundary line.
- Adjust Instrument: If the reading does not match 28.0% Brix, adjust your instrument according to its manufacturer's calibration instructions until it reads 28.0%.
- Clean Up: After calibration, thoroughly clean the instrument's prism or sample well with distilled water and a soft, lint-free cloth. Dry completely.
Note: Always refer to your specific instrument's user manual for detailed calibration procedures, as steps may vary.
Maintenance and Storage
- Recap Immediately: After dispensing the required amount, immediately recap the solution bottle tightly to prevent evaporation and contamination.
- Storage Conditions: Store the solution in a cool, dry place, away from direct sunlight and extreme temperatures. Ideal storage temperature is typically room temperature.
- Avoid Contamination: Do not return used solution to the bottle. Always use a clean dropper or pipette to dispense the solution.
- Shelf Life: Observe the expiration date printed on the packaging. Using expired calibration solution may lead to inaccurate calibration.
Troubleshooting
If you encounter issues during calibration:
- Inconsistent Readings: Ensure the instrument and solution have reached thermal equilibrium. Check for air bubbles or insufficient solution on the prism/sample well.
- Solution Contamination: If you suspect the solution is contaminated, discard it and use a fresh aliquot.
- Instrument Malfunction: If the instrument cannot be calibrated to 28.0% Brix despite following all steps, consult your instrument's user manual for troubleshooting or contact the instrument manufacturer for support.
Specifications
| Parameter | Value |
|---|---|
| Product Name | Brix Calibration Solution |
| Model Number | 300007 |
| Brix Concentration | 28% |
| Brand | Sper Scientific |
| Form | Liquid |
| Package Information | Bottle (available in box of 10) |
Support and Warranty
For technical assistance or inquiries regarding this product, please contact Sper Scientific customer support. Information regarding product warranty, if applicable, can typically be found on the product packaging or on the official Sper Scientific website.
Visit the official Sper Scientific Store for more products and information.





