Introduction
The YSI ProOBOD Self-Stirring Optical BOD Probe is designed for accurate and efficient measurement of Biochemical Oxygen Demand (BOD). This probe features optical technology for dissolved oxygen measurement and an integrated stirring mechanism to ensure consistent sample homogeneity during analysis. This manual provides essential information for the proper setup, operation, and maintenance of your ProOBOD probe.
Key Features
- Smart Sensor Technology: The sensor retains calibration data within the probe, simplifying setup and ensuring accuracy.
- User-Replaceable Sensor: Features a low-maintenance, user-replaceable sensor with an expected lifespan of over one year.
- Durability and Serviceability: Designed for reliability and ease of service, contributing to a lower cost of ownership and reduced downtime.
- Quiet Operation: The integrated stirring mechanism operates with extremely quiet performance.
Setup
Before initial use, carefully inspect the probe for any visible damage. Ensure all connections are clean and secure.
Connecting the Probe to a Meter
- Identify the appropriate port on your compatible YSI handheld or benchtop meter for the ProOBOD probe cable.
- Align the connector on the probe cable with the meter's port.
- Gently push the connector into the port and twist the locking ring (if present) until it is finger-tight to secure the connection. Do not overtighten.
Operating Instructions
Proper operation ensures accurate BOD measurements. Always refer to your specific meter's manual for detailed instructions on navigation and data logging.
Calibration
- Ensure the probe is clean and free of debris.
- Perform a 100% saturation calibration in air-saturated water or a zero-oxygen calibration using a zero-oxygen solution, as required by your measurement protocol.
- Follow the calibration prompts on your connected YSI meter.
BOD Measurement Procedure
- Prepare your BOD samples according to standard methods (e.g., dilution, seeding).
- Carefully insert the ProOBOD probe into the BOD bottle, ensuring the stirring mechanism is submerged and does not touch the bottom or sides of the bottle.
- Initiate the measurement sequence on your connected meter. The probe's internal stirrer will activate automatically.
- Record the initial dissolved oxygen (DO) reading.
- Incubate the BOD bottles for the specified period (typically 5 days) at a controlled temperature (e.g., 20°C).
- After incubation, re-insert the probe into each bottle and record the final DO reading.
- Calculate BOD based on the difference between initial and final DO readings, accounting for dilution and seed correction.
Maintenance
Regular maintenance ensures the longevity and accuracy of your ProOBOD probe.
Cleaning the Probe
- After each use, rinse the probe thoroughly with deionized water to remove any sample residue.
- If fouling occurs, gently wipe the optical sensor window with a soft, lint-free cloth or cotton swab. For stubborn deposits, use a mild detergent solution or follow specific cleaning instructions provided by YSI.
- Ensure the stirring mechanism is free of obstructions.
Sensor Replacement
The optical sensor cap is user-replaceable and has an expected life of over one year. Refer to the YSI ProOBOD sensor replacement guide for detailed instructions. Always use genuine YSI replacement parts.
Storage
- Store the probe in a clean, dry environment when not in use.
- Ensure the sensor cap is protected from physical damage.
- Avoid storing the probe in direct sunlight or extreme temperatures.
Troubleshooting
This section addresses common issues you might encounter with your ProOBOD probe.
| Problem | Possible Cause | Solution |
|---|---|---|
| Inaccurate DO readings | Fouled sensor cap; expired calibration; damaged sensor. | Clean sensor cap; recalibrate the probe; replace sensor cap if damaged or expired. |
| Stirrer not operating | Cable not properly connected; obstruction in stirrer; internal fault. | Check cable connection; remove any obstructions; contact YSI support if problem persists. |
| No communication with meter | Loose cable connection; incompatible meter; damaged cable. | Ensure cable is securely connected; verify meter compatibility; inspect cable for damage. |
Specifications
| Attribute | Detail |
|---|---|
| Model Number | 626400 |
| Probe Type | Self-Stirring Optical BOD Probe |
| Cable Length | 2 Meter |
| Measurement Principle | Optical Dissolved Oxygen |
| Sensor Life | +1 year (user-replaceable) |
| Item Weight | 1.61 pounds (approx. 0.73 kg) |
| Package Dimensions | 12.3 x 6.2 x 3.3 inches (approx. 31.2 x 15.7 x 8.4 cm) |
| Manufacturer | YSI |
Warranty and Support
The YSI ProOBOD Self-Stirring Optical BOD Probe is backed by a 2-year warranty, ensuring reliability and serviceability. This warranty covers defects in materials and workmanship under normal use.
For technical assistance, troubleshooting, or warranty claims, please contact YSI customer support or visit the official YSI website for detailed support resources and contact information.