1. Product Overview
VWR Superfrost Plus Micro Slides are designed for enhanced tissue adherence in various laboratory applications. These slides feature a unique pretreatment process that electrostatically attracts and binds frozen and formalin-fixed tissue sections, eliminating the need for traditional adhesives or subbing solutions.
Key Features and Advantages
- Enhanced Adherence: Electrostatic attraction ensures strong binding of tissue sections, preventing loss during processing.
- Reduced Background Staining: Designed to minimize background staining in standard Hematoxylin and Eosin (H&E) procedures.
- Versatile Application: Effectively binds difficult cytologic preparations and improves tissue adherence in microwave staining protocols.
- Optimized for Advanced Techniques: Prevents tissue loss in immunoperoxidase procedures and optimizes in situ hybridization techniques.
- Time-Saving: Eliminates the need for manual subbing of slides, streamlining laboratory workflows.
Figure 1: Outer packaging of VWR Superfrost Plus Micro Slides. This image displays the product box as it typically appears when received, highlighting the brand and product name.
Figure 2: VWR Superfrost Plus Micro Slides within their protective packaging. This image shows the individual slides bundled inside the box, ready for laboratory use.
2. Setup and Preparation
Before using the VWR Superfrost Plus Micro Slides, ensure your workspace is clean and organized. No special preparation of the slides themselves is required as they are pretreated.
Required Materials (Not Included)
- Microtome or cryostat for sectioning tissue.
- Pre-heated water bath (distilled water recommended).
- Tissue samples (frozen or formalin-fixed).
- Oven for drying slides (optional, depending on protocol).
Figure 3: Close-up of Superfrost Plus Micro Slides packaging. This image shows the product packaging, including details such as catalog number (12-550-15), size (25 x 75 x 1.0mm), and quantity (approximately 1/2 gross). Note that while the image displays 'Fisherbrand', the product is supplied by VWR.
3. Operating Instructions
Follow these steps for optimal use of VWR Superfrost Plus Micro Slides in your laboratory procedures:
- Prepare Tissue Sections: Float your 2 to 5 micron thick tissue sections on a pre-heated water bath. Ensure the water bath is filled with distilled water. Do not add any glue or subbing solution to the water bath, as the Superfrost Plus slide pretreatment eliminates the need for these components.
- Mount Sections: Carefully mount your tissue sections onto the Superfrost Plus slides. Tissue bonding begins quickly due to the slide's properties, so precise placement on the first attempt is recommended.
- Dry Slides: Completely dry the mounted slides at room temperature. It is recommended to drain them vertically. After air drying, slides may be heated in an oven according to your specific protocol.
- Water Quality: While tap water may be substituted for distilled water in your water bath, it is strongly advised to revert to distilled water if you observe any loss of tissue sections during the process.
Figure 4: Detailed instructions and advantages of Superfrost Plus slides. This image provides a visual reference for the recommended usage steps and highlights the benefits of using these specialized slides for various staining and hybridization techniques.
4. Maintenance and Storage
Proper handling and storage ensure the longevity and effectiveness of VWR Superfrost Plus Micro Slides.
- Storage: Store slides in their original packaging in a cool, dry place, away from direct sunlight and extreme temperatures.
- Handling: Always handle slides by their edges to avoid contaminating the working surface with oils or debris from fingers.
- Cleanliness: Ensure slides are free from dust or lint before use. Do not wash or clean slides prior to initial use, as this may compromise the electrostatic coating.
- Rotation: It is important to rotate stock, using older slides first to maintain optimal performance.
5. Troubleshooting
This section addresses common issues encountered during the use of Superfrost Plus Micro Slides.
| Problem | Possible Cause | Solution |
|---|---|---|
| Tissue sections detaching from slide. |
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| High background staining. |
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6. Product Specifications
| Attribute | Detail |
|---|---|
| Product Name | Superfrost Plus Micro Slide |
| Brand | VWR |
| ASIN | B0018EKRAU |
| Slide Dimensions | 25 x 75 x 1.0 mm (approximate, based on packaging image) |
| Quantity per Pack | Approximately 72 slides (1/2 gross) |
| Special Feature | Positively Charged for Immuno and Special Stains |
| Manufacturer | VWR |
| Package Dimensions | 4.02 x 3.82 x 1.1 inches |
| Package Weight | 13.62 ounces |
7. Warranty and Support
Warranty Information
Specific warranty details for VWR Superfrost Plus Micro Slides are typically provided at the point of purchase or within the product packaging. For detailed warranty terms and conditions, please refer to the documentation accompanying your purchase or contact VWR customer service directly.
Customer Support
For technical assistance, product inquiries, or support, please contact VWR customer service. Contact information can usually be found on the VWR official website or on your purchase invoice.
- VWR Official Website: us.vwr.com (for general information and contact details)
- Product Inquiries: Refer to your supplier or VWR sales representative.