1. Introduction and Overview
This manual provides detailed instructions for the OPTO-EDU A13.2263 series of trinocular microscopes, which includes biological, metallurgical, and fluorescent models (A12.2263, A13.2263, A16.2263 respectively). These microscopes are designed for advanced laboratory and research applications, offering high-quality optical performance and versatile illumination options.
The A13.2263 series features an ECGO Infinity Plan Mutual Achromatic Optical System, ensuring clear and precise imaging. Key components include a trinocular wide-field head, ultra-wide field eyepieces, and various objective lenses tailored for specific applications.
2. Specifications
The following table details the specifications for the A12.2263, A13.2263, and A16.2263 microscope models. A "●" indicates inclusion, "○" indicates an optional component, and "/" indicates not applicable.
| Item | Description | A12.2263 Biological | A16.2263 Fluorescent | A13.2263 Metallurgical | Model No. |
|---|---|---|---|---|---|
| Optical System | ECGO Infinity Plan Mutual Achromatic Optical System, Confocal 45mm | BF,DF,PH,POL | BF,FL | BF,POL | |
| Main Body | C63F Main Body: 5 Holes Coded Inwards Nosepiece, Double Layer Stage (76×55mm, Nano Anti-Wear Ceramic Coating Plate, Right Hand XY Moving Knob, Damping Adjustment Ring), Coaxial Coarse & Fine Focusing (Range 25mm, Coarse Stroke 17.6mm, Fine Stroke 0.1m, Fine Scale 1μm, Safety Stop Lock Ring & Tension Adjustable Ring), Built-in Transmit Kohler Illumination (LIM System, Brightness Memory, ECO Auto-Off) | ● | ● | ● | A54.2201-C63F |
| Head & Adapter | Trinocular Wide Field Head, Light Split: (50/50), FN=22mm | ● | ● | ● | A53.2201-CT63 |
| 1.0x C-Mount + Focus Adapter Ring, For Sensor 2/3"~4/3" | ○ | ○ | ○ | A55.2201-1xC | |
| 0.63x C-Mount, For Sensor 1/1.8"~ 2/3" | ○ | ○ | ○ | A55.2201-63xC | |
| 0.5x C-Mount, For Sensor 1/2"~ 1/1.8" | ○ | ○ | ○ | A55.2201-05xC | |
| Eyepiece | Ultra Wide Field 10x/23mm, Diopter Adjustable -2~+8°, With Eyecup | ● | ● | ● | A51.2201-1023 |
| PLCN Infinity Plan Achromatic Objective (Cover Glass 0.17) | 4X, N.A=0.10, W.D=17mm, OFN=23 | ● | ● | / | A51.2202-4 |
| 10X, N.A=0.25, W.D=10mm, OFN=23 | ● | ● | / | A51.2202-10 | |
| 20X, N.A=0.40, W.D=3mm, OFN=23 | ● | ● | / | A51.2202-20 | |
| 40X, N.A=0.65, W.D=0.6mm, OFN=23 | ● | ● | / | A51.2202-40 | |
| 60X, N.A=0.80, W.D=3mm, OFN=23 | ○ | ○ | / | A51.2202-60 | |
| 100X, N.A=1.25, W.D=0.25mm, OFN=23 | ● | ● | / | A51.2202-100 | |
| MPLN Infinity Plan Metallurgical Objective (No Cover Glass) | |||||
| MPLN Infinity Plan Metallurgical Objective | 5x, N.A=0.16, W.D=12mm, OFN=22, M20.32 | / | / | ● | A5M.2210-5 |
| 10x, N.A=0.3, W.D=10mm, OFN=22, M20.32 | / | / | ● | A5M.2210-10 | |
| 20x, N.A=0.45, W.D=12.5mm, OFN=22, M20.32 | / | / | ● | A5M.2210-20 | |
| 50x, N.A=0.8, W.D=3mm, OFN=22, M20.32 | / | / | ● | A5M.2210-50 | |
| 100x, N.A=0.9, W.D=1mm, OFN=22, M20.32 | / | / | ● | A5M.2210-100 | |
| Condenser | Abbe Condenser, N.A=1.1, 4x~100x | ● | ● | ● | A56.2201-11 |
| Achromatic Aplanatic Condenser, N.A=1.4, 10x~100x | ○ | ○ | ○ | A56.2201-14 | |
| Swing-out Condenser N.A=0.9, 1.25x~100x (1.25x~4x When Swing Out) | ○ | ○ | ○ | A56.2201-S | |
| Transmit Illumination | SUPERMLED 10W LED Light House, Life Time 60000 Hours, Wide Color Gamut 4500K Color Temperature | ● | ● | ● | A56.2204-1 |
| Reflect Fluorescent Illumination | Epi Fluorescent LED Illuminator, 1 Band, UV | ○ | A16.2263-UV | / | A5F.2202-UV |
| Epi Fluorescent LED Illuminator, 1 Band, B | ○ | A16.2263-B | / | A5F.2202-B | |
| Epi Fluorescent LED Illuminator, 1 Band, G | ○ | A16.2263-G | / | A5F.2202-G | |
| Epi Fluorescent LED Illuminator, 2 Bands, UV+B | ○ | A16.2263-UVB | / | A5F.2202-UVB | |
| Epi Fluorescent LED Illuminator, 2 Bands, UV+G | ○ | A16.2263-UVG | / | A5F.2202-UVG | |
| Epi Fluorescent LED Illuminator, 2 Bands, B+G | ○ | A16.2263-BG | / | A5F.2202-BG | |
| Epi Fluorescent LED Illuminator, 3 Bands, UV+B+G | ○ | A16.2263-UVBG | / | A5F.2202-UVBG | |
| Reflect Metallurgical Illumination | Epi Metallurgical LED Illuminator, White Color LED Light House, With Metallurgical BF Module, With Polarizing Slot | / | / | ● | A5M.2201-KM |
| Dark Field | Dark Field Condenser (Dry), N.A=0.92, 10x~100x | ○ | ○ | / | A5D.2201-D |
| Dark Field Condenser (Oil), N.A=1.4, 10x~100x | ○ | ○ | / | A5D.2201-I | |
| Phase Contrast (UPLFLN Infinity Plan Semi-APO FL Phase Contrast Objective) | Universal Turret Phase Contrast Condenser, Support BF, DF, PH1, PH2, PH3 | ○ | ○ | / | A5C.2201 |
| Green Filter For Phase Contrast Dia.45mm | ○ | ○ | / | A5C.2201-G45 | |
| 4x, N.A=0.15, CG=0.17, W.D=12mm, OFN=26.5 | ○ | ○ | / | A5C.2212-4 | |
| 10x, N.A=0.30, CG=0.17, W.D=9mm, OFN=26.5 | ○ | ○ | / | A5C.2212-10 | |
| 20x, N.A=0.65, CG=0.17, W.D=2.3mm, OFN=26.5 | ○ | ○ | / | A5C.2212-20 | |
| 40x, N.A=0.90, CG=0.17, W.D=0.7mm, OFN=26.5 | ○ | ○ | / | A5C.2212-40 | |
| Camera (Optional) | 3.0M USB 2.0 Camera, Sony 1/2" Color CMOS, Work With 0.5x C-Mount | ○ | ○ | ○ | A59.2271-3M |
| 6.3M USB 3.0 Camera, Sony 1/1.8" Color CMOS, Work With 0.5x/0.63x C-Mount | ○ | ○ | ○ | A59.2271-6.3M | |
| 20M USB 3.0 Camera, Sony 1" Color CMOS, Work With 1x C-Mount | ○ | ○ | ○ | A59.2271-20M | |
| 30M, USB3.0, Aptina 1/2.2" Color CMOS, With Software, With 0.5x C-Mount | ○ | ○ | ○ | A59.2273-30M | |
| 8.0M USB 3.0 Fluorescent Camera, Sony 1" Color CMOS, Work With 1x C-Mount | / | ○ | / | A59.2272-8M | |
| 12.8M USB3.0 Fluorescent Camera, Sony 1" Color CMOS, Work With 1x C-Mount | / | ○ | / | A59.2272-12.8M | |
| Other | Immersion Oil 8ml, Reflect Ratio=1.518, Low Viscosity (135CST) | ● | ● | / | A50.2201-1 |
| Allen Key 4#, 3# | ● | ● | ● | A50.2204-3 | |
3. Components and Accessories
The microscope system consists of a main body, trinocular head, eyepieces, objectives, and various optional accessories to enhance functionality.
- Main Body (A54.2201-C63F): Includes the nosepiece, working stage, focusing mechanism, and transmit illumination.
- Trinocular Head (A53.2201-CT63): Features a wide field of view and a 50/50 light split for simultaneous observation and camera attachment.
- Eyepiece (A51.2201-1023): Ultra Wide Field 10x/23mm with diopter adjustment (-2~+8°) and eyecups.
- Objectives:
- PLCN Infinity Plan Achromatic Objectives (for biological/fluorescent): 4X, 10X, 20X, 40X, 100X (oil immersion).
- MPLN Infinity Plan Metallurgical Objectives (for metallurgical): 5x, 10x, 20x, 50x, 100x.
- Condenser: Abbe Condenser (N.A=1.1) is standard, with optional Achromatic Aplanatic and Swing-out condensers.
- Illumination: SUPERMLED 10W LED for transmit illumination. Epi Metallurgical LED Illuminator for metallurgical models and various Epi Fluorescent LED Illuminators for fluorescent models.
- Optional Accessories: C-mount adapters for cameras, Dark Field condensers, Phase Contrast condensers and objectives, Polarizing filters, and various USB cameras.
4. Setup Instructions
- Unpacking: Carefully remove all components from the packaging. Retain packaging for future transport or storage.
- Main Body Placement: Place the main body (A54.2201-C63F) on a stable, level surface, away from direct sunlight, vibrations, and excessive dust.
- Head Installation: Gently insert the trinocular head (A53.2201-CT63) into the top port of the main body. Secure it with the set screw if present.
- Eyepiece Insertion: Insert the Ultra Wide Field 10x/23mm eyepieces (A51.2201-1023) into the ocular tubes of the trinocular head.
- Objective Installation: Rotate the nosepiece to allow easy access. Screw the desired objective lenses (e.g., PLCN Infinity Plan Achromatic Objectives) into the nosepiece in ascending order of magnification.
- Condenser Adjustment: Ensure the Abbe Condenser (A56.2201-11) is correctly seated and centered beneath the stage.
- Power Connection: Connect the power cord to the microscope and a suitable power outlet.
- Camera Setup (Optional): If using a camera, attach the appropriate C-mount adapter (e.g., A55.2201-05xC) to the trinocular port, then attach the camera (e.g., A59.2271-3M) to the C-mount. Refer to the camera's specific manual for software installation.
5. Operating Instructions
- Power On: Turn on the main power switch. The built-in SUPERMLED 10W LED transmit illumination will activate.
- Adjust Illumination: Use the illumination control knob to set the desired brightness. The Light Intensity Management (LIM) System and Brightness Memory Function will retain your settings.
- Place Specimen: Place your specimen slide on the mechanical stage, securing it with the stage clips. Use the Right Hand XY Moving Knob to position the specimen over the light path.
- Select Objective: Rotate the 5-hole coded inwards nosepiece to select the lowest power objective (e.g., 4X).
- Focusing:
- Use the Coaxial Coarse Focusing knob to bring the specimen into approximate focus. The focusing range is 25mm with a coarse stroke of 17.6mm.
- Use the Fine Focusing knob for precise focusing. The fine stroke is 0.1m with a fine scale of 1μm.
- Adjust the diopter on the eyepieces for individual eye focus.
- Condenser Adjustment: Adjust the Abbe Condenser's aperture diaphragm and height for optimal contrast and resolution.
- Higher Magnification: Once focused at low power, switch to higher power objectives. Minor fine focus adjustments may be needed. For the 100X objective, apply a drop of immersion oil (A50.2201-1) directly onto the specimen slide before lowering the objective into the oil.
- Metallurgical/Fluorescent Operation:
- Metallurgical (A13.2263): Activate the Epi Metallurgical LED Illuminator. Ensure the polarizing slot is correctly configured if using polarization.
- Fluorescent (A16.2263): Activate the appropriate Epi Fluorescent LED Illuminator (UV, B, G, or combinations) and select the corresponding filter cube.
- Power Off: After use, turn off the illumination and main power switch.
6. Maintenance
- Cleaning Optics: Use only specialized lens cleaning paper and solution for objectives and eyepieces. Gently wipe in a circular motion. Never use abrasive materials.
- Immersion Oil: After using the 100X oil immersion objective, immediately clean off all immersion oil from the objective lens and the specimen slide using lens cleaning solution. Failure to do so can damage the lens.
- General Cleaning: Wipe the microscope body with a soft, dry cloth. For stubborn dirt, a slightly damp cloth can be used, followed by drying. Avoid using organic solvents.
- Dust Protection: Always cover the microscope with a dust cover when not in use.
- Storage: Store the microscope in a cool, dry place, away from direct sunlight and corrosive chemicals.
- Mechanical Adjustments: Use the provided Allen keys (A50.2204-3) for any necessary minor adjustments to mechanical parts, as instructed by a qualified technician or specific guides.
- LED Illumination: The SUPERMLED 10W LED light source has a long lifespan (60,000 hours) and generally requires no maintenance.
7. Troubleshooting
| Problem | Possible Cause | Solution |
|---|---|---|
| No illumination | Power cord disconnected, power switch off, brightness knob set to minimum. | Check power connection, turn on switch, increase brightness. |
| Image blurry or out of focus | Incorrect focusing, objective not fully engaged, dirty objective/eyepiece, immersion oil on dry objective. | Use coarse and fine focus, rotate nosepiece to click objective into place, clean optics, remove oil from dry objectives. |
| Dark field or uneven illumination | Condenser not centered, aperture diaphragm closed too much, light path obstructed. | Center condenser, open aperture, check for obstructions on stage or light source. |
| Dust or spots in field of view | Dust on eyepiece, objective, or specimen. | Clean eyepieces and objectives. Check specimen for dust. |
| Stage movement stiff | Accumulated dirt, tension adjustment too tight. | Clean stage mechanism. Adjust damping adjustment ring if present. |
8. Warranty and Support
The OPTO-EDU A13.2263 series microscopes come with a 3-year warranty, ensuring reliability and peace of mind. OPTO-EDU is committed to providing high-quality optical products and excellent customer service.
For any inquiries, technical support, or to order specific models and accessories not listed, please contact us:
- WhatsApp: +86 18711341578
- WeChat: +86 18711341578
- Email: 304276083@qq.com
We welcome OEM orders and accept small orders. Our products hold complete certifications, including CE, ensuring quality and compliance.
