1. Introduction
The Wondfo YG101 Vet Fluorescence Immunoassay Analyzer is a state-of-the-art veterinary clinical examination device designed for rapid, accurate, and reliable quantitative analysis of various biomarkers in animal samples. This immunoassay system is equipped with internal temperature control and an LCD touch screen, making it an efficient tool for laboratories, hospitals, and clinics. It supports testing for canine, feline, and equine specimens, providing essential diagnostic information within minutes.
2. Safety Information
Please read all safety instructions carefully before operating the device to ensure safe and proper use. Failure to follow these guidelines may result in injury or damage to the instrument.
- Always operate the analyzer in a stable, clean, and well-ventilated environment.
- Ensure the power supply matches the device's requirements (DC12V).
- Avoid spilling liquids on the device. In case of spillage, immediately disconnect power and clean thoroughly.
- Do not attempt to open or repair the device yourself. Refer to qualified service personnel for maintenance.
- Dispose of used test cards and samples according to local biohazard waste regulations.
- The device is designed for professional veterinary use only.
- The disinfecting type for this device is Ultraviolet Light. Follow appropriate UV safety protocols if using this feature.
- Adhere to safety standard EN 149 -2001+A1-2009.
3. Package Contents
Carefully unpack the box and verify that all items listed below are present and undamaged. If any items are missing or damaged, contact your supplier immediately.
- Wondfo YG101 Vet Fluorescence Immunoassay Analyzer
- Power Adaptor and Cable
- Pipettes (e.g., 10μL, 75μL)
- Veterinary FIA Rapid Quantitative Test Kits (e.g., cProg, TSH, cCrp)
- User Manual (this document)
- Other relevant documentation
4. Device Overview
The Wondfo YG101 analyzer features a compact design with an intuitive interface for ease of use.
- Front Panel: Features a large LCD Touch Screen for operation and result display.
- Test Card Slot: Located on the front/side for inserting prepared test cards.
- ID Card Slot: For loading specific test ID cards.
- Rear Panel (Connectivity):
- ON/OFF Switch: Main power switch.
- DC12V Input: Power adapter connection.
- USB Ports: For data transfer and peripheral connections.
- COM1/COM2 Ports (RS232): Serial communication ports.
- PC/MAC Port (Internet): For network connectivity and data management (HIS/LIS connectivity).
- Internal Thermal Printer: For printing test results directly from the device.
5. Setup
- Unpacking: Carefully remove the analyzer and all accessories from the packaging. Retain the original packaging for future transport or storage.
- Placement: Place the analyzer on a flat, stable, and dry surface, away from direct sunlight, excessive heat, or strong vibrations. Ensure adequate space around the device for ventilation and access to ports.
- Power Connection: Connect the provided power adapter to the DC12V input port on the rear of the analyzer. Plug the power cable into a grounded electrical outlet.
- Initial Power On: Flip the ON/OFF switch on the rear panel to the 'ON' position. The device will power on and initiate its startup sequence.
- Language and Time Settings: Follow the on-screen prompts to select your preferred language, set the date, and time.
- Network Connection (Optional): If network connectivity is required for HIS/LIS integration or data management, connect an Ethernet cable to the PC/MAC port. Configure network settings via the on-screen menu.
6. Operating Instructions
The Wondfo YG101 analyzer is designed for ease of use, following a simple four-step procedure for quantitative immunoassay testing.
- Prepare the Analyzer:
- Ensure the analyzer is powered on and has completed its self-check.
- Navigate to the main testing interface on the LCD touch screen.
- Load ID Card:
- Locate the appropriate ID card for the specific test you intend to perform (e.g., Canine Prog, TSH, CRP).
- Insert the ID card into the designated ID card slot on the device. The analyzer will read the test parameters.
- Prepare the Test Card and Sample:
- Obtain a new, unopened test card for the desired assay.
- Using a pipette, accurately add the required volume of sample (e.g., serum, plasma, whole blood, urine, tissue fluid) to the sample well of the test card. Refer to the specific test kit instructions for exact sample volume and preparation.
- Insert Test Card:
- Once the sample is applied to the test card, immediately insert the prepared test card into the test card slot on the analyzer.
- Ensure the card is inserted correctly and fully seated.
- Start Analysis and Read Results:
- The analyzer will automatically detect the inserted card and begin the analysis.
- The reaction time typically ranges from 3 to 15 minutes, depending on the specific test.
- Once the analysis is complete, the results will be displayed on the LCD touch screen.
- You can print the results using the internal thermal printer if desired.
7. Test Parameters and Reference Ranges
The Wondfo YG101 analyzer supports a wide range of veterinary tests. Below is a table detailing common test items, required sample quantities, reaction times, linear ranges, and reference ranges. Always refer to the specific test kit insert for the most current and detailed information.
| Category | Cat. No. | Test Item | Quantity | Reaction Time | Linear Range | Reference Range |
|---|---|---|---|---|---|---|
| Hormone | V403 | T4 | 75μL | 15min | 0.67-100nmol/L (0.52-77.5μg/dL) | Canine 15-50nmol/L (1.2-3.9μg/dL) Feline 10-50nmol/L (0.8-3.9μg/dL) |
| V404 | TSH | 75μL | 15min | 0.1-100mIU/L (0.005-2.56ng/mL) | Canine 0-37mIU/L (0-0.68ng/mL) Feline 0-21mIU/L (0-0.52ng/mL) | |
| V402 | Canine Prog (cProg) | 75μL | 15min | 10-200ng/mL (3.18-63.6nmol/L) | 10-200ng/mL (3.18-63.6nmol/L) | |
| Special Markers | V407 | HbA1c | Whole blood 10μL | 5min | 3%-14.5% | 3%-4.5% |
| V406 | D-Dimer | Whole blood 15μL, plasma 10μL | 15min | 100-10000ng/mL | 0-250ng/mL | |
| V408 | fPL | 75μL | 15min | 50-2000μg/L | <200μg/L | |
| V409 | fPL | 75μL | 15min | 0.5-50μg/L | <3.5μg/L | |
| Inflammation | V412 | CCV-C | 10μL | 10min | 0.1-10mg/L | <1.2mg/L |
| V413 | fCyC-C | 10μL | 10min | 3-300ng/L | <7mg/L | |
| V410 | CNT-proBNP | 75μL | 10min | 150-6000pmol/L | <800pmol/L | |
| V411 | fNT-proBNP | 75μL | 10min | 50-1000pmol/L | <150pmol/L | |
| V400 | Canine CRP (cCRP) | 10μL | 3min | 2-250mg/L | 0-10mg/L | |
| Inflammation | V401 | Feline SAA (fSAA) | 10μL | 10min | 2-300mg/L (μg/mL) | 0-8mg/L (μg/mL) |
| V435 | Equine SAA (eSAA) | 10μL | 10min | / | <0.5μg/L | |
| FIV Activity (Ab) | V430 | FCV Ab | 10μL | 10min | 0.5-100U | <0.5U is level 0; 0.5-1.0U is level 1; 1.0-2.0U is level 2; 2.0-5.0U is level 3; 5.0-10.0U is level 4; >10.0U is level 5. |
| V431 | FPLV Ab | 10μL | 10min | 0.5-100U | <0.5U is level 0; 0.5-1.0U is level 1; 1.0-2.0U is level 2; 2.0-5.0U is level 3; 5.0-10.0U is level 4; >10.0U is level 5. | |
| V432 | FHV Ab | 10μL | 10min | 0.5-250U | <0.5U is level 0; 0.5-1.0U is level 1; 1.0-2.0U is level 2; 2.0-5.0U is level 3; 5.0-10.0U is level 4; >10.0U is level 5. | |
| Immuno | V420 | ICH Ab | 10μL | 10min | 0.5-1200U | <0.5U is level 0; 0.5-1.0U is level 1; 1.0-2.0U is level 2; 2.0-5.0U is level 3; 5.0-10.0U is level 4; >10.0U is level 5. |
| V421 | CPV Ab | 10μL | 10min | 0.5-100U | <0.5U is level 0; 0.5-1.0U is level 1; 1.0-2.0U is level 2; 2.0-5.0U is level 3; 5.0-10.0U is level 4; >10.0U is level 5. | |
| V422 | CDV Ab | 10μL | 10min | 0.5-100U | <0.5U is level 0; 0.5-1.0U is level 1; 1.0-2.0U is level 2; 2.0-5.0U is level 3; 5.0-10.0U is level 4; >10.0U is level 5. | |
| V428 | RV Ab | 10μL | 10min | 0.2-10IU/mL | 0-0.5IU/mL is low level | |
| Infectious Disease | V423 | CCV Ag | Fecal sample 75μL | 10min | / | Positive: infected with the virus; Weak positive: low viral loads. It is suspected to be infected with the virus. It is recommended to combine with clinical symptoms and comprehensive diagnosis based on clinical symptoms. |
| V424 | CDV Ag | Conjunctional secretion sample 75μL | 10min | / | Negative: No infection. | |
| V425 | CPV Ag | Fecal sample 75μL | 10min | / | Negative: No infection. |
Note: Sample Type: Whole blood/serum/plasma is recommended to use serum or plasma, except for HbA1c (whole blood). Level 0: No immunization is recommended. Level 1: Low titer antibodies in the body, immunization is recommended. Level 2: Low titer antibodies in the body. Level 3 and 4: High titer antibodies in the body. Level 5 and 6: High titer antibodies in the body. *Ongoing: FCV Ag, FPLV Ag, FHV Ag.
8. Maintenance
- Cleaning: Regularly clean the exterior of the analyzer with a soft, damp cloth. Do not use abrasive cleaners or solvents. For disinfection, the device supports Ultraviolet Light. Follow standard UV safety procedures.
- Screen Care: Use a soft, lint-free cloth specifically designed for touchscreens to clean the LCD.
- Storage: When not in use, store the analyzer in a cool, dry place, ideally between 4-30°C. Protect it from dust and direct sunlight.
- Calibration: Perform routine Quality Control (QC) checks as recommended by the test kit manufacturer and your laboratory's protocols to ensure accurate results.
- Software Updates: Check for available software updates periodically to ensure optimal performance and access to new features.
9. Troubleshooting
This section addresses common issues you might encounter. For problems not listed here, or if solutions do not resolve the issue, please contact technical support.
- Device does not power on:
- Check if the power cable is securely connected to both the analyzer and the electrical outlet.
- Ensure the ON/OFF switch on the rear panel is in the 'ON' position.
- Verify the power outlet is functional.
- Error message on screen:
- Note down the exact error code or message.
- Refer to the specific test kit instructions or the device's on-screen help for guidance.
- If the issue persists, contact technical support with the error details.
- Inaccurate or inconsistent results:
- Ensure proper sample collection and preparation techniques are followed.
- Verify that the correct ID card and test card are used for the assay.
- Check the expiry date of the test kits.
- Perform a Quality Control (QC) check with known controls.
- Ensure the analyzer is operating within the recommended temperature range (4-30°C).
- Printer not working:
- Check if the thermal paper is loaded correctly and there is enough paper.
- Ensure the printer mechanism is free from obstructions.
- Connectivity issues (USB/RS232/Internet):
- Verify that cables are securely connected.
- Check network settings on the device and ensure the network is active.
- Ensure correct drivers are installed on the connected PC, if applicable.
10. Specifications
| Feature | Detail |
|---|---|
| Model Number | YG101 |
| Type | Veterinary Clinical Examination Device, Dry Fluorescence Immunoassay Analyzer |
| Disinfecting Type | Ultraviolet Light |
| Safety Standard | EN 149 -2001+A1-2009 |
| Specimen Compatibility | Canine, Feline, Equine |
| Sample Types | Serum, plasma, whole blood, urine, tissue fluid |
| Power Source | DC12V (Machinery) |
| Material | Metal, Plastic |
| Connectors | RS232, USB, Internet |
| Weight | 2.8 kg |
| Dimensions (L x W x H) | 270mm x 238mm x 146mm |
| Operating Temperature | 4-30°C |
| Features | LCD Touch Screen, Multi-lingual interface, Internal Thermal Printer, Built-in Temperature Control, Full Traceability, QC Available, HIS/LIS Connectivity |
11. User Tips
- Optimal Performance: For consistent and accurate results, ensure the analyzer is always used within its specified operating temperature range of 4-30°C.
- Sample Integrity: Always use fresh samples or properly stored samples according to test kit instructions to avoid degradation that could affect results.
- Regular Cleaning: A quick wipe-down of the exterior and screen after each use can prevent buildup and maintain device longevity.
- Kit Management: Keep track of test kit expiry dates. Expired kits can lead to unreliable results.
12. Warranty and Support
The Wondfo YG101 Vet Fluorescence Immunoassay Analyzer comes with a manufacturer's warranty. For specific warranty terms and conditions, please refer to the documentation provided with your purchase or contact your supplier.
After-sales Service: Online technical support is available for assistance with setup, operation, troubleshooting, and any other product-related inquiries. Any defective products can be replaced within the warranty period.
For support, please contact your authorized distributor or the manufacturer's customer service department.
