dynamic BIOSENSORS AS-2-Gb-lfs Adapter Strand
Key Features
- Adapter strand 1 – Gc for functionalization of helix ® Adapter Chip Spot 1.
- Compatible with helix ® Adapter Chip.
- Includes Adapter strands for 50 regenerations.
- Ideal for MIX&RUN sample preparation.
- Adapter strand 1 carries a hydrophobic green dye (Gc) with a neutral net charge.
Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: AS-1-Gc
Table 1. Contents and Storage Information
| Material | Cap | Concentration | Amount | Buffer | Storage |
| Adapter strand 1 – Gc | Black | 400 NM | 5 x 100 µL | TE40 [1] | -20°C |
For research use only.
This product has a limited shelf life, please see expiry date on label.
To avoid many freeze thaw cycles please aliquot the nanolayer.
Preparation | MIX&RUN
n-solution hybridization of adapter and ligand strands:
- Mix Adapter strand 1 – Gc (400 NM) and conjugated Ligand strand (500 NM) at 1:1 ratio (v/v).
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix solution of step 2 and Adapter strand 1 – Gc – lfs (200 nM) at 1:1 ratio (v/v).
Solution is ready to use for biochip functionalization.
Stability of the solution is related to the stability of the ligand molecule.
Table 2. Additional material for functionalization of spot 1 and reference spot 2.
| Material | Concentration | Buffer | Related Product Name | Order No |
| Ligand strand carrying the conjugated ligand | 500 NM | PE40 [ | helix ® Amine Coupling Kit | HK-NHS-1 |
| Adapter strand 1 – Gc – lbs. | 200/250 NM | TE40 [1] | Adapter strand 2 with green dye Gc prehybridized with ligand-free strand | AS-2-Gb-lfs |
Example
Required volume for 3 functionalization’s: 100 µL with a final concentration of 100 nM.
| Vial 1 | Vial 2 | |
| Adapter strand 1 – Gc (400 NM) |
Conjugated Ligand strand (500 NM) | Adapter strand 1 – Gc – lfs (200/250 NM) |
| 25 µ | 25 µL | 50 µL |
After incubation time, mix vial 1 and vial 2 to obtain 100 µL of ready-to-use DNA solution.
Customer Support
Contact
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany. ©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
Documents / Resources
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dynamic BIOSENSORS AS-2-Gb-lfs Adapter Strand [pdf] User Manual AS-2-Gb-lfs, AS-2-Gb-lfs Adapter Strand, Adapter Strand, Strand |
