Hygiena LogoBAX® System Real-Nguva PCR Assays
STEC Suite Hygiena KIT2021 System Real Time PCR Assays - iconHygiena KIT2021 System Real Time PCR AssaysQUA 18/11-12/20
DZIMWE DZIMWE NZIRA DZEKUOngorora
YEKURIMA
http://nf-validation.afnor.org/en

Kuongorora Assay - Chikamu KIT2021 (D14642964) ye stx uye eae
KIT ZVIRI MUKATI
96 PCR machubhu ane mapiritsi (2 masaga e6 x 8 mitsetse)
96 flat optical caps (12 x 8 mitsetse)
1 bhodhoro reprotease (400 μL)
2 mabhodhoro elysis buffer (12 mL)
Panel 1 Yekuongorora - Chikamu KIT2008 (D14642970)
zveE. coli O26, O111, O121
KIT ZVIRI MUKATI
48 PCR machubhu ane mapiritsi (1 bhegi re6 x 8 mitsetse)
96 flat optical caps (12 x 8 mitsetse)
1 bhodhoro reprotease (400 μL)
2 mabhodhoro elysis buffer (12 mL)
Panel 2 Yekuongorora - Chikamu KIT2009 (D14642987)
zveE. coli O45, O103, O145
KIT ZVIRI MUKATI
48 PCR machubhu ane mapiritsi (1 bhegi re6 x 8 mitsetse)
96 flat optical caps (12 x 8 mitsetse)
1 bhodhoro reprotease (400 μL)
2 mabhodhoro elysis buffer (12 mL)

CHINANGWA KUSHANDISA

Magadzirirwo echikafu nemarabhoritari akabatana anogona kushandisa BAX® System STEC suite senzira inokurumidza uye yakavimbika yekuona pathogenic Shiga inobudisa muchetura E. coli (STEC) mumhando dzakasiyana dzezvokudya, hemp, uye maruva ecannabis. Imwe neimwe-chaiyo-nguva PCR assay mu STEC suite yakagadzirirwa kuburitsa pachena hongu/hapana mhedzisiro pazvimiro zvakaderera se10 CFU/mL mushure mekupfumisa. Iyo yekuongorora assay inoona STEC virulence genes (stx1, 4 stx2, uye eae) ine nyore, single-s.tage enrichment; maviri-panel assays anogona zvino kushandisa lysate yakafanana kuona kana STEC-screening positive iriwo yakanaka kune imwe yepamusoro nhanhatu STEC serogroups (E. coli O26, O45, O103, O111, O121, uye O145). Nenguva yekugadzirisa inosvika maminitsi makumi mashanu neshanu muBAX® System Q55 chiridzwa, nzira inodzosa mibairo inofananidzwa netsika nzira, asi nenguva inokurumidza kukurumidza mhedzisiro. BAX® Systems dzakagadzirirwa kushandiswa nevashandi vane hunyanzvi hwemurabhoritari vanotevedzera yakajairwa microbiology murabhoritari maitiro, kusanganisira kubata kwakachengeteka uye kuraswa kwezvinhu zvingangoita pathogenic. Iyo rabhoritari inofanirwa kutevedzera yakanaka marabhoritari maitiro (ona ISO 7 standard). Munda Wekushandisa: Dhata yakawanikwa kubva kuBAX® System haifanirwe kushandiswa kuongororwa kwevanhu kana kurapwa kwevanhu. Zvishandiso hazvitenderwe neUnited States Food and Drug Administration kana chero imwe US ​​kana isiri yeUS inodzora agency kuti ishandiswe mukuongororwa kana kurapwa kwevanhu. Iyo BAX® Sisitimu haifanirwe kushandiswa seyo chete hwaro hwekuongorora kuchengetedzwa kwezvigadzirwa kuti zviburitswe kune vatengi. Ruzivo rwunogadzirwa rwunongoshandiswa chete pamwe chete nechirongwa chenguva dzose chemushandisi chesimbisiro yemhando. Hazvibvumirwi kuongororwa nekliniki. Shandisa kutsvaga nekusimudzira, kuvimbiswa kwemhando, uye kutonga kwemhando pasi pekutariswa kwevanhu vane hunyanzvi.
ZVINOTAURWA YEMETHODZO
Ona iyo BAX® System Mushandisi Yekushandisa kwepamusoroview yekuti nzira yeBAX® System inoshandisa sei otomatiki, chaiyo-nguva Polymerase Chain Reaction (PCR) tekinoroji.
ZVINOKOSHA
BAX® System Real-Nguva PCR Assays - STEC suite
STEC Screening assay ye stx uye eae (Chikamu KIT2021 [D14642964]);
STEC Panel 1 assay yeE. coli O26, O111, O121 (Chikamu KIT2008
[D14642970]); kana
STEC Panel 2 assay yeE. coli O45, O103, O145 (Chikamu KIT2009
[D14642987])
BAX® System yekutanga-up package. (zvekushandisa uye zvekushandisa zvekusvika 192 bvunzo)

  • BAX® System Q7 cycler/detector uye komputa yekushandira
  • Mabhuroko ekudziisa ane ekuisa* anokwanisa kuchengetedza 37±2ºC uye
    95±3ºC
  • Mabhuroko ekutonhodza ane anoisa*
  • PCR chubhu inobata
  • Capping / decapping zvishandiso
  • Anochinjika mechanical pipettes (5-50 µL; 20-200 µL)
  • Kudzokorora pipette
  • Multi-channel pipette (8 chiteshi - 5-50 μL)
  • Cluster machubhu ane caps uye racks
  • Pipette mazano ane zvipingamupinyi
  • Magurovhosi e nitrile asina upfu
    *The Automated Thermal Block (Catalog No. MCH2023 [D14614252]) inogona kushandiswa panzvimbo yekudziisa uye inotonhodza zvidhinha. Dumbu nemabhegi.
    Incubator inokwanisa kuchengetedza tembiricha yekupfumisa yakananga pakati pe39-42°C uye tembiricha yekupfumisa mukati me ±2°C.

Cherechedza: Hutano Canada, AOAC, uye AFNOR Certification zviyero zvinoda incubator inokwanisa kuchengetedza ± 1°C.
Enrichment media (Ona BAX® System User Guide kuti uwane ruzivo)

  • Tryptic soy broth (TSB) (Magadzirirwo anoenderana nematrix akaedzwa. Kuti uwane rumwe ruzivo, ndapota tarisa STEC protocol muBAX® System User Guide)
  • BAX® System MP media – Catalog No. MED2003 (D12404925) (2.5 kg)
  • Yakaputirwa Peptone Mvura (BPW)
  • Kaviri Simba BPW

Cherechedza: Kune nzira yeNF-Validation, ndapota cherechedza kuti kune gadziriro dze master solution, unofanirwa kutevedzera mirairo kubva kuEN ISO 6887 zviyero.
KUCHENGETA UYE HUPENYU HUPENYU

  • Reagents uye PCR machubhu ane mapiritsi anofanira kuchengetwa mufiriji pa2-8°C. Usaomesa nechando.
  • Reagents dzinofanirwa kushandiswa nezuva rekupera stamped pamapepa ega ega.
  • Mushure mekunge protease yawedzerwa kune lysis buffer, hupenyu hwesherufu hwemhinduro ndeye mavhiki maviri kana yachengetwa pa 2-2 ° C.
  • Kana uchichengeta PCR machubhu ane mapiritsi mukiti yakashama kweanopfuura mavhiki matatu, namira Mylar bag rePCR machubhu mubhegi hombe rine desiccant kana chitoro pa3ºC mune desiccation unit.

ZVESANGANO

Iyo BAX® System nzira inosanganisira sample enrichment process inosimbisa kukura kwehutachiona hunogona kusvika kumatanho anooneka. Nekuti utachiona hunogona kukonzera kurwara kwevanhu, matanho ekuchengetedza akakodzera anofanirwa kutorwa pakubata samples, media, reagents, glassware, nezvimwe zvinhu uye midziyo inogona kusvibiswa neangangoita pathogenic bacteria. Mareagents anoshandiswa neBAX® System assays haafanire kuisa njodzi kana akashandiswa sekurairwa. Usati washandisa chigadzirwa ichi, ndapota review iyo Safety Data Sheets (SDS) inosanganisirwa neyako BAX® System yekutenga uye inowanikwawo pa www.nmushok.net. Tarisa kune yako saiti maitiro ekubata zvakachengeteka kwezvinhu pakupisa kwakanyanya.
ZVINODIWA SOFTWARE
Usati washandisa iyi assay kekutanga, isa iyo yazvino vhezheni yeBAX® System software, wobva wamhanyisa mushumo wekutarisa kutarisa kuti "Real Time STEC Screen stx, eae", "Real-Time STEC Screen stx chete", " Chaiyo-Nguva STEC Panel 1 O26, O111, O121” uye “Real Time STEC Panel 2 O45, O103, O145” inoonekwa mune rondedzero yecalibration. files. Ona "Troubleshooting Calibration" muBAX® System User Guide kuti uwane ruzivo. Kana rondedzero yemushumo isina zvibodzwa izvi unofanirwa kudzokorora chiridzwa cheQ7 kurodha madhayi anodiwa. Iva neshuwa yekubvumidza nguva yakakwana yekupedzisa calibration (inenge 1.5 kusvika 2 maawa) usati watanga bvunzo. Kuti uwane mirairo uye mazano ekugadzirisa chiridzwa, ona BAX® System User Guide.
ENRICHMENT PROTOCOL

  1. Gadzirira Enrichment Broth maererano nemirairo yemugadziri. Wona iyo BAX® System Mushandisi Yekushandisa kune yakajairwa kuvandudza midhiya mabikirwo.
  2. Unganidza uye Upfumise Sampmás
    Nzira Yakatenderwa neAOAC
    • Nyama yemombe mbishi (375 g): Homogenize sample ine 1.5 L isati yadziya (46°C) glucose-ine TSB ine 2mg/L novobiocin. Isa pa 41°C kwemaawa 12-24.
    • Nyama yemombe yakakangwa nesoya (325 g): Homogenize sample ine 975 mL isati yadziya (35°C) glucose-ine TSB ine 10 g/L casamino acids uye 8 mg/L novobiocin. incubate pa41°C* kwemaawa 12-24.
    • Kucheka nyama yemombe mbishi (325 g): Gwira zvinyoro nyoro sample ine 975 mL yekamuri-tembiricha-glucose-ine TSB ine 10 g/L casamino acids uye 8 mg/L novobiocin. Isa pa 41°C* kwemaawa 15-24.
    • Mucheka wemombe yemombe (375 g): TSB - Gently massage sample ine 1.5 L isati yadziya (46°C) glucose-ine TSB. Isa pa 41°C* kwemaawa 12-24. MP Media – Geza zvinyoro nyoro sample ine 1.5 L prewarmed (46°C) BAX® System MP media. Isa pa 41°C* kwemaawa 1224.
    • Mhuru yemombe (25 g): BPW Media - homogenize ample ine 225 mL isati yadziya (37°C) BPW. Isai pa 37 ° C kwemaawa 10-24. NTSB Media – Homogenize sample ine 225 mL isati yadziya (37°C) NTSB + amino acids. Isai pa 42 ° C kwemaawa 10-24.
    • Hupfu (25 g): Homogenize sample ine 225 mL isati yadziya (42°C) mTSB ine 2mg/L novobiocin. Isa pa 42°C kwemaawa makumi maviri nemana. *Cherechedza: Tembiricha yeincubation inofanira kuchengetedzwa pakati pe24 ºC ne39 ºC pachiyedzo ichi.
    • Kamba yakaomeswa neruva rehemp: Sanganisa 10 g ruva ne90 mL prewarmed (37-42°C) BPW. Kukwizwa nemaoko kweminiti imwe uye gadzira pa1 ° C kwemaawa 42-22. Nzira Yakagamuchirwa neAFNOR Zvikamu zvekuyedza zvinorema kupfuura 25 g hazvina kuongororwa muchirevo cheNF VALIDATION. Nekugadzirira kwekutanga kumiswa, tevera mirairo ye EN ISO 6887 standard.
    • Nyama yemombe mbishi (25 g): BPW Media Homogenize sample ine 225 mL isati yadziya (41.5°C) BPW. Isa pa 41.5 ° C kwemaawa 10-24. MP Media – Homogenize sample ine 225 mL isati yadziya (41.5°C) MP media. Isa pa 41.5 ° C kwemaawa 7-24.
    • Zvigadzirwa zvemukaka zvakasvibirira (25 g): Simba rakapetwa kaviri (DS) BPW Media – Homogenize sample ine 225 mL isati yadziya (41.5°C) DS BPW. Isai pa 41.5 ° C kwemaawa 20-24.
    • Miriwo (25 g): MP Media – Homogenize sample ine 225 mL isati yadziya (41.5°C) MP media. Isa pa 41.5 ° C kwemaawa 8-24.

TEST PROTOCOL - ALL ASAYS

3. Gadzirira Midziyo
3.1 Batidza mabhuroko ekudziya kusvika pa37ºC uye 95ºC*.
3.2 Iva nechokwadi chekuti mabhuroko ekutonhodza akatonhorwa kusvika pa2-8ºC*.
*Kana uchishandisa Automated Thermal Block, tevera mirairo iri muThermal Block User Guide yekumhanyisa chirongwa cheGram-Negative.
3.3 Simba pachiridzwa cheQ7 uye tanga iyo BAX® System application.
3.4 Gadzira rack file (ona Bhuku reMushandisi kuti uwane ruzivo). CHINOKOSHA CHINOKOSHA CHESTEC KUCHENZA NE "STX CHETE": Imwe nzira yekudonhedza-pasi yechinangwa inowanikwa yekumhanyisa chirongwa che "stx chete". Ona iyo BAX® System Mushandisi Gwaro kuti uwane ruzivo.
4. Ita Lysis
4.1 Dimbura machubhu emapoka.
4.2 Nyora uye ronga machubhu emasumbu mureki zvinoenderana nereki file.
4.3 Gadzirira lysis reagent nekuwedzera 150 µL protease kune imwe 12 mL bhodhoro re lysis buffer.
4.4 Kuendesa 200 µL yakagadzirirwa lysis reagent kune yega yega cluster chubhu.
4.5 Kuendesa 20 µL kwakapfumisa sample kune inoenderana cluster chubhu.
4.6 Kupisa pa37°C kwemaminitsi makumi maviri.
4.7 Kupisa pa95°C kwemaminitsi makumi maviri.
4.8 Kutonhorera pa2-8°C kweinenge maminitsi mashanu.
5. Hydrate PCR Mahwendefa
5.1 Tanga chiridzwa nekusarudza RUN FULL PROCESS kubva kuOPERATION menyu.
5.2 Isa PCR chubhu rack pane inotonhora (2-8°C) PCR inotonhodza block.
5.3 Ronga mitsetse yePCR machubhu zvinoenderana neraki yako file.
5.4 Bvisa zvivharo kubva pachikamu chekutanga chechubhu nemudziyo wekuchekeresa.
5.5 Shandura 30 µL lysate (kubva padanho 4.8) kupinda muPCR machubhu, wobva wasimbisa nemakepisi akatsetseka.
5.6 Dzokorora nemitsetse yasara yemachubhu ePCR kusvikira mapiritsi ese ePCR aiswa mvura.
Cherechedza: Mapiritsi ePCR anofanirwa kuvharwa uye kuvharwa zvakare pakarepo mushure mekubvisa zvivharo kubva kumachubhu ePCR.
6. Amplify uye Detect
6.1 Pakukurumidza "Kugadzirira Rack Load", tinya bhatani NEXT wovhura dhirowa rezviridzwa.
6.2 Isa rack yemachubhu ePCR pamusoro pematsime ari mudhirowa, uye tarisa kuti machubhu akagara zvakanaka here.
6.3 Vhara dhirowa wodzvanya bhatani ZVINOTEVERA kuti utange kugadzira otomatiki.
Cherechedza: Kana zvichidiwa, lysate yakasara inogona kuiswa chisimbiso uye kuchengetwa pa 2-8 ° C kuti iwedzere kuongororwa neBAX® System STEC suite uye / kana realtime E. coli O157: H7 assay.
7. Review Results
Mhedzisiro yemhando inoratidzwa segridi yemavara-akachekwa zvidhori muhafu yepamusoro yechidzitiro: Hygiena KIT2021 System Real Time PCR Assays - Mufananidzo

Hygiena KIT2021 System Real Time PCR Assays - icon 1Girinhi (-) #NAME
Hygiena KIT2021 System Real Time PCR Assays - icon 2Tsvuku (+) #NAME
Hygiena KIT2021 System Real Time PCR Assays - icon 3Yero (?) = Indeterminate result*
Hygiena KIT2021 System Real Time PCR Assays - icon 4Yero (?)
ine tsvuku
slash
= Chikanganiso chechiratidzo*

*Tarisa kuchikamu chekugadzirisa matambudziko muBhuku reMushandisi kuti ubatsirwe.
Kuongorora Assay Mhedzisiro (stx uye eae)
Mhinduro yakanaka - Inoratidza kuti zvese eae uye stx zviripo mune iyo sample. The ampLification plot inoratidza kukwira kwezvinangwa zvestx (blue) uye eae (girinhi).
Negative mhedzisiro - Inoratidza kuti musanganiswa we stx uye eae haupo mune iyo sample. Kana imwe chete yezvinangwa zve stx kana eae iripo, iyo sample inonzi negative.
Kuongorora Assay Mhinduro ("STX CHETE")
Mhinduro yakanaka - Inoratidza kuti stx iripo mune iyo sample. The amplification chirongwa chinoratidza kukwira kweiyo stx (yebhuruu) chinangwa. The eae target inofuratirwa. **
Negative mhedzisiro - Inoratidza kuti stx haipo mune iyo sample. The eae target inofuratirwa.
**Kushandisa Test Protocol iri pamusoro, yakachengetwa lysates ye positive Screening samples inogona kumhanyiswa nePanel 1 uye Panel 2 assays yekuona chaiyo "Big 6" serogroup(s), kana iripo.
mhedzisiro yakanaka - Inoratidza kuti imwe kana yakawanda yePanel 1 tarisiro iripo mune iyo sample:

  • E. coli O26 - the ampLification plot inoratidza kukwira kweO26 (goridhe) chinangwa
  • E. coli O111 - the ampLification plot inoratidza kukwira kweO111 (grey) chinangwa
  • E. coli O121 - the ampLification plot inoratidza kukwira kweO121 (yepepuru) target anel 1 Assay Results (E. coli O26, O111, O121)

Negative mhedzisiro - inoratidza kuti hapana chimwe chezvinangwa zvePanel 1 chiripo mune icho sample.
Panel 2 Assay Mhinduro (E. coli O45, O103, O145)
Mhedzisiro yakanaka - Inoratidza kuti imwe kana kupfuura yePanel 2 tarisiro iripo mune iyo sample:

  • E. coli O45 - the ampLification plot inoratidza kukwira kweO45 (magenta) chinangwa
  • E. coli O103 - the ampLification plot inoratidza kukwira kweO103 (brown) chinangwa
  • E. coli O145 - the ampLification plot inoratidza kukwira kweO145 (turquoise) chinangwa

Negative mhedzisiro - Inoratidza kuti hapana chimwe chezvinangwa zvePanel 2 chiripo mune icho sample.

KUFUNGA

Nzira Yakatenderwa neAOAC
Kana uchida, BAX® nzira yakapfumisa sampLes inogona kusimbiswa nereferensi tsika nzira yakakodzera iyo sample type, senge:

  • US FDA Bacteriological Analytical Manual (BAM)
  • USDA FSIS Microbiology Laboratory Guidebook (MLG)
  • Health Canada Compendium yeAnalytical Methods
  • International Organisation yekumira (ISO)
  • AOAC SMPR 2020.002

Nzira Yakagamuchirwa neAFNOR
Zvese sampizvo zvinoonekwa sezvakanaka neBAX® System nzira inofanirwa kusimbiswa mune imwe yeiyi nzira dzinotevera:

  • Yakananga plating - Streak 10 µl yekupfumisa paCT-SMAC uye
    CHROMagar STEC. Isa ma plates kwemaawa 22-26 pa37ºC. Edza 1-5 yakajairika makoroni kubva pane imwe yakasarudzika agar ndiro ne:
    o Latex agglutination uye BAX® System Real Time STEC Screening assay kana
    o BAX® System Real Time STEC Screening assay uye ingave BAX® STEC Panel 1 kana BAX® STEC Panel 2 kana BAX® System Real-Time Assay yeE. coli O157:H7.
  • Immuno-concentration (IMS) - Ita IMS uye streak 10 µl yekupfumisa paCT-SMAC uye CHROMagar STEC. Isa ma plates kwemaawa 22-26 pa37ºC. Edza 1-5 yakajairika makoroni kubva pane imwe yakasarudzika agar ndiro ne:
    o Latex agglutination uye BAX® System Real Time STEC Screening assay kana
    o BAX® System Real Time STEC Screening assay uye ingave BAX® STEC Panel 1 kana BAX® STEC Panel 2 kana BAX® System Real-Time Assay yeE. coli O157:H7.

Muchiitiko chemhedzisiro yekusawirirana (kufungidzira kwakanaka neimwe nzira uye isina kusimbiswa neimwe yenzira dzakatsanangurwa pamusoro) marabhoritari inofanirwa kutevedzera matanho anodiwa kuti ive nechokwadi chechokwadi chemhedzisiro yakawanikwa.
KURASA
Decontaminate zvinhu uye kurasa marara ane biohazardous pane yako saiti maitiro uye sezvinodiwa nemubatanidzwa, nyika uye yemuno mirau.

KUSIMBISA

Iyo BAX® System Real-Nguva PCR Maongororo eSTEC (Kuongorora, Panel 1 uye Panel 2) yakasimbiswa neAOAC Research Institute sePerformance Tested Method SM #091301. Kuita kwemaassays aya kwaive reviewed neAOAC-RI uye yakawanikwa ichiita kune zvakatemwa nemugadziri. Zvidzidzo zvekusimbisa zvechikafu uye hemp uye cannabis maruva, yakaratidza BAX® System senitivity uye yakasarudzika yakaenzana kana nani pane yereferenzi tsika yakavakirwa nzira. Iyo USDA Chikafu Chekuchengetedza uye Inspection Service (USDA-FSIS) yatora iyo BAX® System STEC suite yekutarisa zvigadzirwa zvenyama uye chitunha nemaponji ezvakatipoteredza. Ona FSIS Microbiology Laboratory Guidebook (MLG) Method #5B.04 kuti uwane humbowo nemaitiro. Ndapota cherechedza kuti kupfumisa uye sampmaprotocol ekugadzirira muMLG anogona kusiyana kubva kune ari muBAX® System zvinyorwa. Iyo BAX® System Real-Time PCR Assay Suite yeSTEC yakasimbiswa #QUA 18/11-12/20 maererano nemitemo yeNF VALIDATION. Zvidzidzo zvekusimbisa zvakaitwa zvinoenderana neEN ISO 16140-2 chiyero chakawana mashandiro ekiti ichi chekuyedza kugutsa mirairo yeNF VALIDATION yenyama yemombe mbishi, zvigadzirwa zvemukaka mbishi nemiriwo. Shanduro yesoftware yakashandiswa panguva yeNF Validation yekudzidza inoratidzwa muNF VALIDATION chitupa cheimwe nzira. Kuti uwane rumwe ruzivo, kusanganisira mazuva echokwadi, ndapota tarisa kune chitupa QUA 18/11- 12/20 chinowanikwa pa http://nf-validation.afnor.org.

RUBATSIRO RWEZVO
Kuti uwane mibvunzo kana makomendi, ndapota taura nemugovera wemunharaunda yako. Iwe unogona zvakare kufonera 800-863-6842 muUS, 1-302-695-5300 kunze
US, kana email [email inodzivirirwa].

KUSANGANISWA KWEVARENDI NESIMBA

ISANGANO: VERENGA UYU KUPIMISWA CHEWARANTY UYE CHIKAMU USATI WASHANDISA BAX® SYSTEM EQUIPMENT, ASSAYS, UYE/KANA MEDIA (“BAX® SYSTEM”). Kana mazwi asingagamuchirwe, zivisa Hygiena nekukasira uye hurongwa huchaitwa hwekudzoserwa kweChishandiso chisina kushandiswa, assays, uye / kana midhiya kuHygiena uye nekudzoserwa kwemutengo wekutenga, mari shoma yekutumira. KUSHANDISWA KWEBAX® SYSTEM EQUIPMENT, ASAYS UYE/KANA MEDIA ZVINOBVIRIRA KUBVUMA KWEMITEMO YESE NEZVINHU ZVINO ZVINHU ZVINHU ZVINHU CHEWARANTY NEZVINHU. Chero mazwi ekuwedzera kana akasiyana muMutengi's fomu rekutenga (s) shanduko yezvinhu uye zvinorambwa.

  1. BAX® System Equipment inofanirwa kushandiswa chete neBAX® System assays.
  2. Kana ikashandiswa neBAX® System assays, BAX® System Equipment inotenderwa kuti ive isina kukanganisa mumidziyo, magadzirirwo uye dhizaini inogona kuoneka pasi pekushandiswa kwakaringana uye kwakaringana mukati memwedzi gumi nembiri (12) kubva pazuva rekuisa kusvika kumushandisi wekutanga. BAX® System assays inotenderwa kuti ienderane nerondedzero yeassay pasi pemamiriro ekushandiswa anotsanangurwa mugwaro remushandisi kusvika pazuva rekupera.amped pazita. BAX® System midhiya inotenderwa kuti isangane neyakajairwa zvirevo mukuita pazuva rekutumira. Hygiena HAIITA IMWE WARRANTI, ZVINOTAURWA KANA ZVINOREVEDZERA, zvinosanganisira, PASINA ZVINOGONESA, Chero WAranti Inopesana nekutyorwa, chero waranti yekutengesera KANA KUKONZERA CHINHU CHINHU CHINHU KANA CHINHU CHETE, CHETE, RIMWE CHETE, CHETE. Mushandisi anotora njodzi yese uye mutoro unobva mukushandiswa kweBAX® System Equipment, assays uye midhiya, ingave ichishandiswa chete kana musanganiswa nezvimwe zvigadzirwa.
  3. BAX® Software: Hygiena inobvumidza kuti kwemazuva makumi matanhatu kubva pazuva rekutanga kushandiswa neMutengi/mushandisi wekupedzisira, BAX® software midhiya haizove isina kukanganisa mune zvekushandisa uye magadzirirwo uye kuti BAX® software ichaita. kuita zvakanyanya zvinoenderana neinoperekedza BAX® software zvinyorwa. KUNZE KWEZVINOTAURWA WARRANTY IRI PAMBERI, HYGIENA HAIITA IMWE WARRANTY, ZVINOTAURWA KANA KUTI ZVINOREVA, zvinosanganisira, PASINA ZVINOGONESA, CHEYI WARRANTY INOPINDA KUKATARWA, CHIPI WARRANTI YEKUTENGWA KANA KUSIYANA, KUSIYANA, KUSIYANA, KUSIYANA, KUSIYANA. , KANA KOSI YEKUBATA. Mushandisi anotora njodzi yese uye mutoro unobva mukushandiswa kweBAX® software, ingave yakashandiswa yega kana musanganiswa nezvimwe zvigadzirwa.
  4. Kurongeka kweBAX® System inogona kukanganiswa nezvinhu izvo Hygiena isingakwanisi kutonga, kusanganisira, pasina muganhu, kushandiswa kweEquipment, assays uye / kana midhiya nenzira inopesana nemamiriro ekushandiswa, maitiro kana maitiro. mirairo inotsanangurwa neHygiena. Nekuda kwehuwandu hukuru hwezvinhu izvo Hygiena isingadzoreki, Hutsanana hahupi vimbiso kana vimbiso yekurongeka kana mhedzisiro inowanikwa kubva mukushandiswa kweBAX® System. Kunyanya, Hygiena inorambira chero waranti kana mhosva uye haitore mutoro chero wekutadza kweBAX® System nekuda, yakazara kana chikamu, nekutadza kwemushandisi: (a) kuchengetedza nemazvo Zvishandiso, (b) kuchengetedza zvakatarwa kushanda kana kuchengetedza. mamiriro, (c) kutevera mirairo yakataurwa, kana (d) kushandisa nzira dzakakodzera dzemicrobiological dzinoenderana nechiyero chekuchengetedza chinogamuchirwa muindasitiri yekuunganidza, kuchengetedza, kubata uye kugadziriraample.
  5. Kunze kukonzeresa kukundikana, senge zvisina kunaka sampkugadzirira, kuchengetwa kusina kunaka kana kurodha reagents, magetsi outages, kana kunze-kwe-kutsanangurwa mamiriro ezvakatipoteredza haana kuvharwa pasi peiyi waranti. Kutadza kwemidziyo kunokonzerwa nekuteuka, kushungurudzwa, kushandiswa zvisizvo, kuregeredza, kana kushanda zvisina kunaka hakuna kuvharwa newarandi iyi. Kugadziridzwa, masevhisi kana kugadziriswa nemapato kunze kwevanopa vane mvumo yeHygiena hakuna kuvharwa neiyi waranti uye, kutaura zvazviri, inobvisa waranti iyi. Mamiriro ezvinhu anopfuura kudzora zvine musoro kweHygiena, kusanganisira moto, kuputika, tsaona, mafashama, dambudziko revashandi kana shor.tage, hondo, chiito kana mvumo nehurumende ipi zvayo, kusakwanisa kuwana zvinhu zvakakodzera, Midziyo, mafuta emoto, simba kana chokufambisa, kana mabasa aMwari haafukidzwi pasi pewarandi iyi.
  6. Iyo BAX® Sisitimu yakagadzirirwa kuyedza chete kuvapo kwezvisikwa zvinotarirwa zvakatsanangurwa mune chaiyo assay. Iyo BAX® Sisitimu yakaedzwa kutarisana nevakawanda, asi kwete ese, madhindindi echinangwa mukati meiyo sample mhando dzakatsanangurwa muzvinyorwa zvevashandisi. Hutsanana, saka, haigone uye haiite chero chinomiririra kana waranti yekuti BAX® Sisitimu inokwanisa kuona zvipenyu zvese mugenus inotangwa, serotype, kana mhando mune chero s.ample source. Saizvozvo, iyo BAX® System haifanirwe kushandiswa seyezve bvunzo yekuburitswa kwezvigadzirwa zvevashandisi, uye haifanirwe kushandiswa seyo chete hwaro hwekuona kuchengetedzwa kwezvigadzirwa zvevashandisi.
  7. MUTENGO/MUSHANDI ANOFANIRA NJODZI DZESE PAKUSHANDISA BAX® SYSTEM UYE HUTANO KANA VAMWE VANOBATSIRA, VANOPARIDZI, VANOPA MAREREENSI KANA VAmiririri HAVACHAVA NEMHOSVA KUMUTENDI/MUSHANDI KANA KUMUMWE MUNHU KANA ZVINHU, ZVINHU, ZVINHU, ZVINHU KANA ZVINHU ZVINOTEVERA KUKABASWA ZVIRIpi, zvinosanganisira, ASI ZVISI ZVINOGONA KUTI, KURASIKIRWA KWEMURIPO KANA KUTI MUNHU, RAKARASIKA KANA KUKATADZWA DHATA KANA KUTI KURASIKA KWEZVO ZVINHU ZVINHU ZVINHU ZVINHU ZVINHU ZVICHIITWA NEKUSHANDISA Utsanana KANA KUVA NEZVINHU ZVINHU UYE ZVINHU ZVINHU ZVINHU. ZVEKUKATADZWA KWAKADA, UYE/KANA KANA ZVICHIFONEKERWA
  8. Iyo yega uye yakasarudzika mushonga wemutengi / mushandisi, UYE CHETE UYE CHETE CHIKWERERO CHEHYGIENA, VAMWE VANOBATSIRA, VANOPASI, VANOPA MAREREENSI KANA VAmiririri kune chero uye zvese zvichemo, zvinosanganisira kutyorwa kweWARRANTY, ZVINOTAURWA, ZVINHU ZVINHU ZVINOTEVERA KUZVI ZVINOTEVERA: (a) Kana Midziyo ikatadza kuenderana neParagraph 2 waranti, Hygiena, pakusarudza kwayo, ichagadzirisa kana kutsiva iyo isingaenderane Equipment nechitsva kana chakagadziridzwa (chakagadziridzwa kana kuvakwazve) chinoshanda chakaenzana Chishandiso kana kudzorera kutenga. mutengo; (b) Kana BAX® Software ikatadza kuenderana neParagraph 3 waranti, Hygiena ichaitsiva pasina muripo; (c) Kune zvimwe zvese zvichemo, Hygiena inogona, pakusarudza kwayo, kudzorera mutengo wekutenga kana kutsiva Equipment, assays kana media; (d) Muzviitiko zvese, mushandisi ane basa rekurongedza uye kudzoserwa kwezvisina-kuenderana, pamwe nekumisikidzwazve kweChishandiso chitsva kana chakagadziridzwa; uye (e) Zvishandiso, maassays kana media hazvidzoserwe pasina mvumo yakanyorwa kubva kuHygiena, uyezve chete nenzira yakatemwa neHygiena. Mutoro wakakura weHygiena, vanobatana nawo, vagovera, uye vanopa marezinesi, uye kunyangwe kana kwete zvichibva mukuregeredza, hazvizopfuure muhuwandu hwakaenzana ne: (a) mutengo wekutenga weBAX® System, assay kana media iyo inopihwa. kukuvara kunodiwa, kana (b) kana iri BAX® Software, mari yakabhadharwa software (kana yakapihwa rezenisi zvakasiyana) kana zviuru makumi maviri nezvishanu zvemadhora ($25,000.00USD). Mutengi/mushandisi achazivisa Hygiena nezve chero chikumbiro mukati memazuva makumi matatu (30) kubva ipapo uye achatanga chero chiito pamusoro peHygiena mukati megore rimwechete (1) rechikonzero chekuita kana neimwe nzira kurambidzwa kubva kune chero kugadzirisa. Hygiena haizove nemhosva yemutengo, kurasikirwa kana mutoro unomuka kubva kune mutengi / mushandisi mashandiro ebhizinesi rake, uye mutengi / mushandisi anobvuma kubhadharira, kudzivirira uye kubata Hygiena nevamiriri vayo vasina mhosva kubva mumutengo wakadaro, kurasikirwa kana mutoro.

Zvinyorwa / Zvishandiso

Hygiena KIT2021 System Chaiyo-Nguva PCR Assays [pdf] Mirayiridzo
KIT2021 System Chaiyo-Nguva PCR Assays, KIT2021, System Chaiyo-Nguva PCR Assays

References

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