What is the purpose of each buffer and how do they contribute to the RNA extraction process? (GALENVS PR0050-12 Extraction Kit User Guide)

Answer

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Sep 22, 2025 - 02:14 PM

The GALENVS PR0050-12 Plant RNA Extraction Kit includes several buffers that play crucial roles in the RNA extraction process:

1. Lysis Buffer: This buffer helps break down the cell membranes and release the RNA from the plant cells. It contains components that disrupt the cell structure and protect the RNA from degradation.

2. PPB (Protein Precipitation Buffer): PPB helps to remove proteins from the sample, allowing for the isolation of pure RNA. It aids in separating RNA from other cellular components.

3. Binding Buffer #1: This buffer facilitates the binding of RNA to the binding beads, separating it from other cellular components that are not of interest for RNA analysis.

4. Wash Buffer #1 and Wash Buffer #2: These buffers are used to wash away impurities and contaminants that may interfere with downstream applications. They help ensure the purity of the isolated RNA.

5. Elution Buffer: Elution Buffer is used to release the purified RNA from the binding beads, making it available for further analysis or downstream applications.

6. DNase Reaction Buffer: This buffer is used in the DNase treatment step to remove any DNA contamination from the RNA sample, ensuring the purity of the extracted RNA.

7. Binding Buffer #2: This buffer is used in conjunction with the binding beads to further purify the RNA sample, removing any remaining contaminants.

8. Wash Buffer #3: Wash Buffer #3 is used for additional washing steps to further purify the RNA sample and remove any residual impurities.

Each buffer in the kit is specifically formulated to perform a critical function in the RNA extraction process, ultimately leading to the isolation of high-quality RNA suitable for various downstream applications such as RT-PCR, gene expression analysis, and sequencing.