8. Click Start to open Protocol Editor Program and execute a run on the ATC 3 4 1 2 10 9 8 7 6 5 Figure 10 Run Protocol 1 Add ( ), Remove (−) stages 2 Remove and arrange order 3 Run Mode drop down 4 Cover Temperature and Sample Volume 5 Temperature, Ramp Rate, Hold Time 6 Cancel 7 Start Run 8 Export SiLA 9 Save 10 Load 1.
Applied Biosystems Automated Thermal Cycler (ATC) | Thermo Fisher Scientific - US
QUICK REFERENCE Automated Thermal Cycler (ATC) System Installation and Use Catalog Numbers A30207, A30208, A31486, A31487, A31488, A31489, A31490, A31491, A33976, A33977 , A33978, A33979, A33980, A33981, and A33982 Pub. No. MAN0016422 Rev. A.0 Note: For safety and biohazard guidelines, see the "Safety" appendix in the Automated Thermal Cycler (ATC) System User Guide (Pub. no. MAN0014558). Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves. Product information Item Computer CMPTR,DELL E6540 RC (PN A25621) Description Quantity 1 Product description The Automated Thermal Cycler (ATC) System is designed for integrating with laboratory automation products of other Plates equipment manufacturers (OEM) for use in automation workflows. The ATC System can also be used as a stand-alone device in conjunction with desktop software. Full-skirted adaptor Package contents plate 1 (Cat. No. A33045) Item Description Quantity Modules Semi-skirted plate adaptor 1 (Cat. No. A33044) ATC block module 1 You will receive one of the following: · ATC 96 well block module (Cat. No. A30207) · ATC 384 well block module (Cat. No. A33976) ATC control module 1 (Cat. No. A30208) Cables You will receive one of the following: · 10 cm cable (Cat. No. A31485) Connector cable · 1 m cable 1 (Cat. No. A31482) · 3 m cable (Cat. No. A31483) Ethernet cable -- 1 AC power cable -- 1 For Research Use Only. Not for use in diagnostic procedures. Location of power points and ports 3 4 5 2 1 Figure 1 Back panel of the ATC control module 1 Power port 2 Power button 3 Ethernet port 4 Automated Thermal Cycler (ATC) System DSUB 5 USB port Use ATC plate adaptors The plate adaptor helps to ensure that the supported consumables (full skirt and semi-skirt plates) are ejected properly, and that the temperature is kept uniform. The Automated Thermal Cycler comes factory configured with a full-skirted adaptor (amber color) already installed. Note: ATC plate adaptors are only installed/required on the 96well block. Adaptors are not required for the 384-well block. 1 Figure 2 Back panel of the ATC block module 1 Automated Thermal Cycler (ATC) System DSUB Connect the hardware 1. Connect the connector cable into the insertion points on the ATC block (Figure 1, item 4) and base module (Figure 2, item 1) Note: Ensure the DSUB connector is securely fastened. 2. Connect the power cable into the back of the ATC control module (Figure 1, item 1) 3. Connect the ethernet cable into the control module (Figure 1, item 3) and into the computer/network that you will be using to control the ATC. 4. Power on the ATC. Figure 3 Automated Thermal Cycler 96-well block with fullskirted plate adaptor installed · To uninstall the full-skirted adaptor, follow the instructions in Figure 4. 1 2 3 Figure 4 Uninstall the full-skirted plate adaptor 1 Slightly push the tabs highlighted in red. 2 Grab the bottom of the adaptor, and tilt up. 3 Remove the adaptor. · To install full-skirted plate adaptor follow the instructions in Figure 5. 2 Automated Thermal Cycler (ATC) System Quick Reference 1 2 4 There are four ejector buttons. Operate the ATC as a stand alone instrument using a computer Make sure all hardware connections are completed "Connect the hardware" on page 2. 3 4 1. Download the ATC desktop software at thermofisher.com and follow the installation instructions found in the software release notes. Note: To fully install the software, the computer will need to be connected to the internet. Once the software is fully installed, disconnect the computer from the internet for best performance with the ATC desktop software. 2. Once the software is installed, click on the ATC Demo icon to launch the software. Figure 5 Install full-skirted plate adaptor 1 Remove half skirted adaptor, if any. place full skirted adaptor by tilting an angle allowing the front to insert under the frame. 2 Push the two tabs allowing it to be inserted into the frame. 3 Ensure the full-skirted plate adaptor sits freely under the frame. 4 Full-skirted plate adaptor is installed. · To install the semi-skirted plate adaptor, follow the instructions in Figure 6. 1 2 Figure 7 Desktop icon of ATC Demo software 1 2 3 4 3 4 Figure 6 Installation of semi-skirted plate adaptors and ejector buttons 1 Slot the semi-skirted plate adaptor to the gap between the Alu top plate and drip pan. 2 Slot in the adaptor and center it. 3 Snap fit the ejector buttons. Automated Thermal Cycler (ATC) System Quick Reference Figure 8 ATC Demo software initial screen 1 Discover 2 A pop up window 3 Identified instrument name 4 Connect 3. Make sure the ATC is powered on and connected via ethernet cable to your computer. Note: It will take approximately 1.5 minutes for the ATC to initialize before it can connect to the software. 4. Once the ATC instrument has initialized, click Discover to identify all devices connected to the same local network. A pop up window will appear. 5. Double click on the identified instrument name. 3 6. Click Connect. Figure 9 ATC Demo software connected to the ATC instrument 7. Hover mouse over the real time monitoring temperature curves to see black window legend with graph related information. 8. Click Start to open Protocol Editor Program and execute a run on the ATC 4 3 3. In the Steps field, specify the temperature set points, ramp rate, and duration. The ramp rate is indicated as a % where 100% corresponds to a peak block ramp rate of 3.5C/s. Type in -1 in the Hold Time field to achieve an infinity hold time. 4. Enter cover temperature and sample volume. 5. Click Start Run to start immediately, or Save for future use. 6. Press Load to display previously protocols. Note: An empty protocol cannot be saved. 7. Click Export SiLA to export protocol for integration with SiLA based software or scheduler. Note: Although SiLA exported protocol is an .xml file it should opened using a text editor in order to see the content, otherwise they might appear empty if opened with a default browser application. When exporting to SiLA format, no white space is allowed in stage label and protocol file name. Only alpha numeric characters and "-", "_" are accepted. Note: A message will notify the user if the Protocol Editor is started while the instrument is already running (Edit Only mode). User can still edit and export a protocol while the software control and monitor the instrument running, without interfering with the current run. 2 1 5 10 9 8 Figure 10 Run Protocol 7 6 1 Add (+), Remove (-) stages 2 Remove and arrange order 3 Run Mode drop down 4 Cover Temperature and Sample Volume 5 Temperature, Ramp Rate, Hold Time 6 Cancel 7 Start Run 8 Export SiLA 9 Save 10 Load 1. In Stages, add or remove protocol stages with the plus and minus signs. 2. Click up and down arrows to arrange the sequence of stages. Specify the number of cycles required for the particular stage. 4 Figure 11 Instrument not ready for run warning Figure 12 Run Protocol (Edit Only) mode Automated Thermal Cycler (ATC) System Quick Reference A run in progress will show the following display: Figure 13 ATC Demo software -Run in progress monitoring If the lid is opened while a run is in progress a warning message will open. Connect to multiple instruments using the desktop software 1. Connect multiple ATC instruments via ethernet cable to your ethernet network, or directly into your computer using a multiport ethernet/network switch. Each ATC will be assigned its own IP address by the network. 2. To contol multiple ATC instruments, open multiple instances of the ATC Demo software by repeatedly double clicking on the software shortcut. In each software instance, discover and connect to a different ATC. 3. To launch a number of instances of the ATC Demo software at the same time, double click the ATC Demo desktop icon (FigureX) repeatedly to open multiple windows of the application. and then select from each specific application window (using Discovery button) the instrument you want to connect to. 4. Click Discover in each specific application window to identify the instrument that you want to connect to. Figure 14 Alert of run in progress 8. Click Abort to end the run. Figure 15 Run ended notification Figure 16 Parallel ATC instruments control and monitoring through multiple instances of ATC Demo software Note: We recommend that you use one computer to control multiple ATC instruments, and we do not recommend that a single ATC is connected to multiple computers. Connect Automated Thermal Cycler to a robotic system Please contact your robotic system manufacturer for information on software compatibility, physical mounting of the ATC on your robot, and licensing information. For the most recent information regarding robot compatibility, driver information, and the full instrument user guide please visit thermofisher.com/atc or thermofisher.com. Automated Thermal Cycler (ATC) System Quick Reference 5 Ordering information Unless otherwise indicated, all materials are available through thermofisher.com. ATC System Item ATC 96 WELL SYSTEM, LAPTOP, 1m cable ATC 96 WELL SYSTEM, LAPTOP, 3m cable ATC 96 WELL SYSTEM, LAPTOP, 10 cm cable ATC 96 WELL SYSTEM, 1m cable ATC 96 WELL SYSTEM, 3m cable ATC 96 WELL SYSTEM, 10 cm cable ATC, 96 WELL BLOCK ATC, 1m cable ATC, 3m cable ATC, 10 cm cable ATC, CONTROL BOX ATC, semi-skirted plate adaptor ATC, full-skirted plate adaptor ATC, 384 WELL BLOCK ATC 384 WELL SYSTEM, LAPTOP,1m cable ATC 384 WELL SYSTEM, LAPTOP, 3m cable ATC 384 WELL SYSTEM, LAPTOP, 10 cm cable ATC 384 WELL SYSTEM, 1m cable ATC 384 WELL SYSTEM, 3m cable ATC 384 WELL SYSTEM, 10 cm cable Cat. No. A31486 A31487 A31488 A31489 A31490 A31491 A30207 A31482 A31483 A31485 A30208 A33044 A33045 A33976 A33977 A33978 A33979 A33980 A33981 A33982 Recommended plastics Item MicroAmpTM EnduraPlateTM Optical 96Well Full-Skirted Plates with Barcode, Clear MicroAmpTM EnduraPlateTM Optical 96Well Full-Skirted Plates with Barcode, Red MicroAmpTM EnduraPlateTM Optical 96Well Full-Skirted Plates with Barcode, Blue MicroAmpTM EnduraPlateTM Optical 96Well Full-Skirted Plates with Barcode, Green MicroAmpTM EnduraPlateTM Optical 96Well Blue Reaction Plates with Barcode MicroAmpTM EnduraPlateTM Optical 96Well Green Reaction Plates with Barcode MicroAmpTM EnduraPlateTM Optical 96Well Red Reaction Plates with Barcode MicroAmpTM EnduraPlateTM Optical 96Well Clear Reaction Plates with Barcode MicroAmpTM EnduraPlateTM Optical 96Well Multicolor Reaction Plates with Barcode MicroAmpTM EnduraPlateTM Optical 96Well Yellow Reaction Plates with Barcode Cat. No. A31732 A31728 A31729 A31727 A31731 4483343 4483349 4483350 4483352 4483354 4483355 4483356 4483395 Quantity 500 50 50 50 50 20 20 20 500 20 5 500 20 Limited product warranty Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of Sale found on Life Technologies' website at www.thermofisher.com/us/en/home/global/terms-and-conditions.html. If you have any questions, please contact Life Technologies at www.thermofisher.com/support. Manufacturer's address: Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256 The information in this guide is subject to change without notice. DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT. Revision history: Pub. No. MAN0016422 Revision A.0 Date 11 April 2017 Description New document Important Licensing Information: This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all applicable Limited Use Label Licenses. ©2017 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. thermofisher.com/support | thermofisher.com/askaquestion thermofisher.com 11 April 2017Antenna House PDF Output Library 6.2.640 (Windows (x64))