NeuroNexus
User Guide for NeuroNexus models including: SmartBox Pro 1024 Lightening Fast Channels
Unique, highly interactive, probe- centric user interface that allows the researcher to remain focused on the experiment at hand. This “Quick Start Guide” ...
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DocumentDocumentSmartBox Pro Radiens Allego V 2.6.7 June 29, 2021 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT Table of Contents Table of Contents 2 Introduction 3 Getting up and running 4 What's in the Box 4 Computer Requirements 4 System Overview 5 Front Panel 5 Back Panel 5 Connect the SmartBox Pro to Your Computer 6 Download and Install the Allego Software Application 7 Windows 7 macOS 7 Allego Interface 8 Allego Dashboard 8 Activating Allego 9 Checking Hardware Connection 11 Training with Allego 12 Quick streaming and saving - Determine if a headstage and probe are connected prop- erly and can stream data 13 NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 2 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT Introduction Congratulations on your purchase of the SmartBox Pro/ Radiens Allego acquisition system! This advanced new system from NeuroNexus enables and accelerates neuroscience research through its unique combination of performance, ease of use, and forward-looking advanced features, which include: l Plug-and-play compatibility with all NeuroNexus probes and electrode arrays l High-quality acquisition and recording of up to 512 channels of neural and bio- logical signals l Unique, highly interactive, probe- centric user interface that allows the researcher to remain focused on the experiment at hand This "Quick Start Guide" is intended to provide a brief overview of the SmartBox Pro/Allego system and some of its basic functions. A note on basic terminology: "SmartBox Pro" refers to the hardware component of the system, i.e., the "SmartBox Pro box." "Radiens Allego" refers to the software application that interfaces with and controls the hardware box. IMPORTANT: Please check the NeuroNexus web site at www.NeuroNexus.com for regular updates of this document and related system documentation. For questions regarding operation of the system or if you have any issues with the SmartBox Pro, please email us at support@NeuroNexus.com. You can also call us at +1.734.913.8858. NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 3 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT Getting up and running What's in the Box l SmartBox Pro unit l AC Power Adapter l USB 3.0 cable l (2) Banana-to-banana cables l (2) BNC-to-BNC cables l (2) BNC-to-Alligator cables l Selected SmartLink headstage amplifier(s) and headstage interface cable(s) that are specified in the order. For more information about our SmartLink headstages please visit our website at www.NeuroNexus.com l (optional) Windows laptop computer that was specified in the order Computer Requirements The computer system requirements include: l Storage: SSD. Traditional magnetic hard disks may not be fast enough in some recording configurations l USB 3.0 l 3rd Generation Intel Core i7 processors (4 cores), or equivalent NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 4 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT System Overview Front Panel Power LED/Button ABCD Headstage Input Ports RE Reference Electrode CE Counter Electrode V/I Potentiostat/Galvanostat Port Back Panel Indicates system status. Proprietary HDMI-style ports for connecting SmartLink headstages to Ports A-D. Banana jack for connection to Reference Electrode (potentiostat/galvanostat). Banana jack for connection to Counter Electrode (potentiostat/galvanostat). Proprietary HDMI-style multifunction port for connection to the SmartBox Pro MUX adapter to provide access to potentiostat and galvanostat functions. DI/DO Digital / Analog Inputs and Outputs BNC connectors for connecting auxiliary signals from other instruments. System Ground Banana jack for connecting the box to a low-noise earth ground point. USB 3.0 USB 3.0 Connector 9VDC DC Power Input Audio Output Connect to a USB 3.0 port on PC/Mac using the supplied USB 3.0 cable. Use only the supplied AC power adapter to provide the required DC voltage input. Stereo audio Aux jack for connection to speakers to monitor the Analog Output (AO) channels. NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 5 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT Connect the SmartBox Pro to Your Computer l Unpack the SmartBox Pro unit from its packaging and place it on a secure surface with adequate ventilation l Plug the AC adapter into a standard (110-220 V) wall outlet and connect its DC power output pin into to the power port of the unit l Connect the USB cable to the SmartBox Pro and a USB 3.0 port on your computer l With the computer on and connected to the internet, press and release the SmartBox Pro power LED switch. Your computer should indicate that it has detected new hardware (Opal Kelly XEM6310-LX45). The necessary drivers should be automatically installed with no further action necessary. If the driver does not install or the Allego recording software cannot detect your SmartBox Pro unit, try downloading and installing the driver from www.NeuroNexus.com. l Connect a SmartLink headstage amplifier to a SmartLink HDMI cable and then connect the other end to one of the SmartBox Pro amplifier ports (ports A-D) l Optional-Use a banana cable to attach the SmartBox Pro ground port to a low-noise earth ground. This may be beneficial for removing extrinsic noise on the signal lines depending on the laboratory environment. (Note: Regardless of the ground port connections, the recording reference and ground signals are provided through the configuration of the SmartLink headstage amplifier(s)) l Note: Do not connect the multiplexer adaptor to ports A through D NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 6 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT Download and Install the Allego Software Application Allego is the software application for interfacing with the SmartBox Pro unit. The latest version of Allego can always be found on the NeuroNexus website. https://neuronexus.com/allego-software-downloads-page/ Once there, select the link for the appropriate operating system and then select "Download" to begin the transfer. Windows Launch the installer. If you receive a warning against running an unrecognized app, select "More Info", then "Run anyway." After the installation completes, Allego will launch. macOS Download the Allego installer. Double-click the installer to launch. Drag the Allego icon into the Applications folder. Open the Applications folder by double clicking the folder icon. In the Applications folder, double-click the Allego icon while holding down the control key (holding down the control key overrides the MacOS security check, which is only needed during the initial launch). After the installation completes, Allego will launch. NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 7 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT Allego Interface Allego Dashboard CONFIGURATION Syst em Elect r odes Signal Processing Configure signal sources, which ports will be used, sampling frequency, and D/A (Outs). Connected probe designs are selected, along with which port and SmartLink headstage will be used. Create various filter types and combine them to create DSP groupings. SIGNAL PROCESSING Monitor Shows the signals currently being streamed. Impedance Impedance testing. VISUALIZATION HD Snapshot Signal Metrics Spike Grid Set t ings Takes a high resolution still picture of signals for closer inspection. Configure spike detection parameters and view key performance indicators/signal quality characteristics. Set spike detection threshold using voltage or standard deviation of background noise from the mean. Displays detected spikes across all channels. Activate the software, set visual themes and set the recording file name and locat ion. NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 8 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT Activating Allego l Launch Allego. Once it has finished initializing and the progress bar disappears, navigate to the left side of the interface and hit the Settings button l Acquisition Settings in the upper-right lists the directory where your recording files will be saved, as well as the root file name of each file l License Status displays the current state of your software activation l If the status is inactive, unknown or expired, you may: 1. Click the "REQUEST TRIAL" button. This will grant a 14-day trial license. 2. Check the emails you have received from NeuroNexus for an 18-digit license key. If you do not have this yet, contact NeuroNexus Customer Service (support@NeuroNexus.com) to request a license key. Paste the 18-digit key into the text box labeled "Activate" and click the arrow. l If this is a trial system, activation will extend the license and unlock other parts of the application l Accept the End User License Agreement to begin using Allego l Your license key allows two computers to run Allego simultaneously. If you need to activ- ate Allego on a different computer, you may deactivate the license on one by choosing the NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 9 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT Deactivate button. This removes the current computer from the allowed list, freeing up the seat to be use with another computer instead. l If your software has been activated but appears inactive, unknown or expired, click the REFRESH button to re-attempt to activate. If this does not work, check your internet connection. NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 10 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT Checking Hardware Connection Navigate to the Dashboard and hit the System button. With the SmartBox Pro on (press and release the SmartBox Pro power LED switch) and connected to SmartLink headstages, your software recognize connected SmartLink headstages automatically. If you are using a different Intan-based system, click on the Mode menu and select the appropriate option. On the right side of the bottom bar in the System tab, you can check the status of connected ports to the SmartLink headstages. Green light indicates which ports are in use. The SmartBox Pro supports simultaneous recording of up to 512 channels across all four ports. For example, below shows the status of two 64 channel SmartLink headstages connected to ports A, and D. The sample rate on the System view can be changed to values between 1 kHz to 30 kHz. NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 11 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT Training with Allego To familiarize yourself with Allego before using it with a SmartBox Pro, return to the Dashboard and select the System icon if the tab is not still open. From the Mode dropdown menu, choose Training: Simulated spike data. This mode simulates having a 64 channel headstage connected on Ports A and D. You may also test filtering options by choosing Training: Sine waves. Sine mode simulates a 64 channel headstage connected on Ports A and C. NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 12 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT Quick streaming and saving - Determine if a headstage and probe are connected properly and can stream data l From the Dashboard, click the Monitor icon l Choose a name for this monitor if desired l Click Stream. Data should begin to populate the window. a. If it does not, navigate to the System view to verify that the SmartBox Pro detects your SmartLink headstage(s) b. You might need to scale the streaming to display the waveforms appropriately. On the bottom of Monitor view, click the Vertical Controls button to increase/decrease the time span, the space between your waveforms, and the magnitude or resolution of your signals. l From the Signal Processing tab of the Dashboard, apply a filter set that is suited for your research. For more information about the filtering, refer to technical document. l To save streamed data, click Record. The streaming and recording status will be displayed in the Status bar visible all throughout the application. NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 13 SmartBox Pro DATA ACQUISITION AND EXPERIMENTAL MANAGEMENT NeuroNexus Technologies, Inc. ©2020 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan, 48108, USA Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | Support@neuronexus.com Page 14