SPAK Polyclonal Antibody (IHC-00686)
SPAK IHC Antibody Rabbit Polyclonal Antigen Affinity Purified Catalog No. IHC-00686 Lot No. IHC-00686-1 Protein ID AAC72238.1 GeneID 27347 APPLICATIONS SPECIES REACTIVITY PRESUMED REACTIVITY AMOUNT CONCENTRATION STORAGE/SHELF LIFE PHYSICAL STATE BUFFER ISOTYPE ORIGIN PRODUCTION PROCEDURES IHC Human Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Gorilla, Chimpanzee and Northern white-cheeked gibbon 100 µl 200 µg/ml 2 - 8° C / 1 year from date of receipt Liquid Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide IgG USA Antibody was affinity purified using an epitope specific to SPAK immobilized on solid support. The epitope recognized by IHC-00686 maps to a region between residue 425 and 450 of human STE20/SPS1-related proline-alanine-rich protein kinase using the numbering given in entry AAC72238.1 (GeneID 27347). APPLICATIONS Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. APPLICATION NOTES Immunohistochemistry 1:100 - 1:500 Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. IHC HUMAN CONTROLS ADDITIONAL INFO In some cases, the antibody may be diluted further than indicated. Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Testicular Seminoma https://www.bethyl.com/product/IHC-00686 Use the link above to view SDS, a current list of citations, and other product specific information. This document certifies that this product has met all of the quality control standards defined by Bethyl Laboratories, Inc. Eric McIntush, PhD | Chief Scientific Officer Date: June 21, 2019 Bethyl Laboratories, Inc. · 25043 West FM 1097 · Montgomery, TX 77356 · 800.338.9579 · 936.597.6111 · 866.597.6105 (FAX) · www.bethyl.com · technical@bethyl.com 1 For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for human or animal consumption. This product may not be resold or modified for resale without the prior written approval of Bethyl Laboratories, Inc. The information provided in this data sheet is believed to be correct but does not purport to be all-inclusive and is intended to be used as a guide. Bethyl Laboratories, Inc. shall not be liable or responsible in any way for use of either this information or the material supplied. Disposal of hazardous material may be subject to federal, state or local laws or regulations. SPAK IHC Antibody IHC-00686 Detection of human SPAK by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SPAK (Cat. No. IHC-00686) used at a dilution of 1:100. Detection: Redfluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100. Detection of human SPAK by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SPAK (Cat. No. IHC-00686) used at a dilution of 1:250. Detection: DAB Detection of human SPAK by immunohistochemistry. Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-SPAK (Cat. No. IHC-00686) used at a dilution of 1:250. Detection: DAB Bethyl Laboratories, Inc. · 25043 West FM 1097 · Montgomery, TX 77356 · 800.338.9579 · 936.597.6111 · 866.597.6105 (FAX) · www.bethyl.com · technical@bethyl.com 2 For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for human or animal consumption. This product may not be resold or modified for resale without the prior written approval of Bethyl Laboratories, Inc. The information provided in this data sheet is believed to be correct but does not purport to be all-inclusive and is intended to be used as a guide. Bethyl Laboratories, Inc. shall not be liable or responsible in any way for use of either this information or the material supplied. Disposal of hazardous material may be subject to federal, state or local laws or regulations.Microsoft Access 2013 Microsoft Access 2013