NovaSeq 6000 Sequencing System Site Prep Guide
1000000019360-06 JPN
イルミナ株式会社
novaseq-site-prep-guide-1000000019360-jpn NovaSeq 6000 Sequencing System
Site Prep Guide
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ILLUMINA PROPRIETARY
NovaSeq 6000 Sequencing System Site Prep Guide
Illumina, Inc. © 2019 Illumina, Inc. All rights reserved. Illumina, Inc. jp.illumina.com/company/legal.html
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NovaSeq 6000 Sequencing System Site Prep Guide
Illumina® NovaSeqTM 6000
X X X X X
NovaSeq 6000 Sequencing System Safety and Compliance Guide 1000000019357
NovaSeq 6000
Custom Protocol Selector
NovaSeq 6000 Sequencing System
Safety and Compliance Guide
1000000019357
RFID Reader Compliance Guide RFID
1000000002699
NovaSeq Series Custom Primers Guide1000000022266
NovaSeq 6000 Sequencing System Guide1000000019358
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NovaSeq 6000 Sequencing System Site Prep Guide
NovaSeq 6000 1 2
1 155 cm61 104 cm41 155 cm61 628 kg1,385
2 84 cm33 122 cm48 102 cm40 176 kg388
1
2 UPS UPS
X 1 X 2 5
X UPS 46 kg100 X UPS 64 kg140 X 31 kg68
X X X X X
X SBS
X X
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NovaSeq 6000 Sequencing System Site Prep Guide
X
X 2
X
X NovaSeq 6000 Sequencing System Guide1000000019358 X NovaSeq 6000 Sequencing System Safety and Compliance Guide
1000000019357 X RFID Reader Compliance Guide1000000002699
X
* 165.6 cm65.2 80.0 cm31.5 94.5 cm37.2 481 kg1,059
* UPS
X /
X
X
X UPS UPS
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NovaSeq 6000 Sequencing System Site Prep Guide
152.4 cm60
76.2cm 30 76.2 cm30
30.5cm12 2 61 cm 24
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NovaSeq 6000 Sequencing System Site Prep Guide
50 m/s VC-A 8 80 Hz 8 80 Hz ISO Operating Room 100 m/s
X
X
X ISO Operating Room
X
X HVAC
X
X 100 cm39.4
X
NovaSeq 6000 Reagent Kit
1 2
SBS
15^ 30^ -25^ -15^ 2^ 8^ 15^ 30^ -25^ -15^
SBS SBS
NovaSeq 6000 Reagent Kit
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NovaSeq 6000 Sequencing System Site Prep Guide
1
SBS
1 1 1 1 1
42.2 cm16.6 20.6 cm8.1
29.5 cm11.6 13 cm5.1
35.6 cm14 20.3 cm8
4.1 cm1.6 2.3 cm0.9
30 cm11.8 12.4 cm4.9
21.1 cm8.3 9.4 cm3.7 2.8 cm1.1 12.4 cm4.9 11.2 cm4.4
PCR
PCR PCR PCR
PCR PCR
X PCR PCR X PCR PCR X PCR PCR X PCR PCR X PCR PCR
PCR
X PCR PCR
X
50/60 Hz 200 240 AC 2,500
200 240 AC 15 A 10%
NovaSeq 6000 Sequencing System Safety and Compliance Guide 1000000019357
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NovaSeq 6000 Sequencing System Site Prep Guide
IEC 60320 C20 Interpower Corporationwww.interpower.com 2.5 m8 AC
2
AS 3112 SAA C1915 A
230 VAC15 A
15 A I
NBR14136 C19 220 VAC16 A 16 A
NBR 14136 N
GB2099 C1916 A
220 VAC16 A
GB 1002GB 2099
I
EU¹
Schuko CEE 7EU1-16p 220 240 VAC16 A Schuko CEE 7/3
C1916 A
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NovaSeq 6000 Sequencing System Site Prep Guide
IS1293 C1916 A
230 VAC16 A
BS546A M
IEC 60320 C1916 A
230 VAC16 A
SI 3216 A H
NEMA L6-30P30 A
200 VAC30 A
NEMA L6-30R
AS 3112 SAA C1915 A
230 VAC15 A
15 A I
NEMA L6-20P C19
208 V16 A
NEMA L6-20R
20 A
IEC60309 316P6 C19 16 A
230 250 VAC16 A IEC60309 316C6
SANS 164-1 C19 16 A
230 VAC16 A
BS546A M
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NovaSeq 6000 Sequencing System Site Prep Guide
SEV 1011 23 J16 A
230 VAC16 A
SEV 1011 23 J
IEC60309 316P6 C19 230 250 VAC16 A IEC60309 316C6
16 A
¹
IEC 60309
UPS UPS
X UPSAPC Smart-UPS X 3000 Rack/Tower LCD 200-240VSMX3000RMHV2U X APC Smart-UPS XSMX120RMBP2U
1.8 kW 2.5 kW × ×
UPS 2,700 W*/ 3,000 VA 200 240 VAC 50/60 Hz IEC-60320 C20 51
34
95 kg210 43.2 cm × 66.7 cm × 17 cm17 × 26.26 × 6.72
* UPS 330 2,700
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NovaSeq 6000 Sequencing System Site Prep Guide
UPS 1
UPS SRT3000RMXLW-IEC SUA3000UXI SRT5KXLJ SRT3000RMXLW-IEC SRT3000RMXLW-IEC SRT3000RMXLW-IEC
SRT96RMBP SUA48XLBP2 SRT192BPJ SRT96RMBP SRT96RMBP SRT96RMBP
APC www.apc.com
UPS
* *
19^ 25^22^ 3^
20 80% 20 60%
2,000 6,500
ISO 9
ISO ISO
* 25^ 80%
dB < 75 dB
1 3.3
2,500
8,530 BTU/h 6,000 BTU/h
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NovaSeq 6000 Sequencing System Site Prep Guide
23 cm10 60%
X X
X 23 cm10
X X
X X
450 CFM 12^
1
NovaSeq 6000
5
5 1,000 mm
17
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NovaSeq 6000 Sequencing System Site Prep Guide
Compute Engine
Compute EngineCE Camera Interface BoardCIBBaseSpace Sequence Hub Compute Engine
Linux
Compute Engine Linux Linux Linux Security-Enhanced LinuxSELinux
Compute Engine
Compute Engine IP 169.254.0.1
X Enhanced Mitigation Experience ToolkitEMET
X
EMET NovaSeq 6000 Sequencing System Guide 1000000019358
X X X X
X X
C:\Illumina Z:\ilmn X Windows Defender Windows
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NovaSeq 6000 Sequencing System Site Prep Guide
NovaSeq 6000 BaseSpace Sequence Hub
BaseSpace Sequence Hub
X X X
NovaSeq 6000
X 1
X
X 1 200 Mb/s X BaseSpace Sequence Hub 1 200 Mb/s
X 1 5 Mb/s
X
X 1 Gb/s
X
X
X CAT-5e CAT-6 3 9.8
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NovaSeq 6000 Sequencing System Site Prep Guide
IP 169.254/16 IPv6 fddc:65e5:66fa::*
2
fddc:65e5:66fa::1/48
169.254/16 IP
1
2
3
NFS IPv6
Compute fddc:65e5:66fa::2/48 Engine
10
BMC IP169.254.0.2 NIC IP169.254.0.1
CE
localhost:*
5555 8081 8080 8090 22801114436232049 590088899980 fddc:65e5:66fa::1/48 fddc:65e5:66fa::2/48
Real-Time Analysis NovaSeq Control Software Universal Copy Service
BaseSpace Sequence Hub Illumina Proactive IP
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NovaSeq 6000 Sequencing System Site Prep Guide
BaseSpace Sequence Hub
Universal Copy Service BaseSpace Sequence Hub Illumina Proactive
{domain}
EU
EU
{domain}.basespace.illumina.com {domain}.api.basespace.illumina.com basespace-data-east.s3-external-1.amazonaws.com basespace-data-east.s3.amazonaws.com instruments.sh.basespace.illumina.com {domain}.euc1.sh.basespace.illumina.com {domain}.api.euc1.sh.basespace.illumina.com euc1-prd-seq-hub-data-bucket.s3-eu-central-1.amazonaws.com instruments.sh.basespace.illumina.com basespace.illumina.com api.basespace.illumina.com basespace-data-east.s3-external-1.amazonaws.com basespace-data-east.s3.amazonaws.com instruments.sh.basespace.illumina.com euc1.sh.basespace.illumina.com api.euc1.sh.basespace.illumina.com euc1-prd-seq-hub-data-bucket.s3-eu-central-1.amazonaws.com instruments.sh.basespace.illumina.com
IP
BaseSpace Sequence HubIllumina Proactive IP
BMC NFS
TCP 228044362359008889 1112049
UDP 623 1112049
CE
229980
IP 169.254.0.2 fddc:65e5:66fa::2/48 fddc:65e5:66fa::1/48 169.254.0.1 169.254.x.x/16
X 10 ID
X
X
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NovaSeq 6000 Sequencing System Site Prep Guide
X GPO
GPO IT X Windows
Remote Desktop ProtocolRDP RDP Illumina Security Best Practices Guide970-2016-016
X
X IP
X
E
Windows Update
Windows
Windows Update X LAN X NAS
X USB
X
Windows Update
ChromeJavaBox RoboCopy
BaseSpace Sequence Hub
BaseSpace Sequence Hub BaseSpace Sequence Hub
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NovaSeq 6000 Sequencing System Site Prep Guide
NovaSeq 6000 SP 300 NovaSeq 6000 SP 500 NovaSeq 6000 S1 300 NovaSeq 6000 S2 300 NovaSeq 6000 S4 300
0.10 TB 0.16 TB 0.37 TB 0.73 TB 2.19 TB
0.20 TB 0.32 TB 0.73 TB 1.43 TB 4.35 TB
CBCL Interop FASTQ BAM gVCF VCF
SP 300 235 GB 0.6 GB 285 GB 265 GB 7 GB
SP 500 392 GB 1 GB 475 GB 442 GB 11.6 GB
S1 300 470 GB 1.2 GB 570 GB 530 GB 14 GB
S2 300 930 GB 2.3 GB 1,125 GB 1,050 GB 28 GB
S4 300 2,800 GB 7.0 GB 3,387 GB 3,160 GB 84 GB
UNC
NovaSeq 6000 BaseSpace Sequence Hub
S2 300 1 10 2 TB SPS1 S4 S2 SP S1 S2 1 10 SP 500 0.25 TB
PF
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NovaSeq 6000 Sequencing System Site Prep Guide
BAM
BAM
VCF gVCF
VCF gVCF
10 / / * 120 / / 10 / /
120 / /
SP 300 2.65 TB 31.8 TB 0.07 TB
0.84 TB
*
SP 500
4.42 TB
53.04 TB
0.12 TB
S1 300
14 TB
163 TB
0.3 TB
1.39 TB 3.6 TB
S2 300 14 TB 168 TB 0.3 TB
3.6 TB
S4 300 42 TB 504 TB 0.9 TB
10.8 TB
NovaSeq 6000 Sequencing System Guide 1000000019358
1 N NaOH 10 mM Tris-HClpH 8.5
0.2 N
PhiX
400 mM Tris-HClpH 8.0
PhiX
500 mL
Tween 20
30 mL
NaOCl
70% 70%
VWR95041-714
VWR21905-026
1.5 mL
VWR20170-038
NaOH
NaOCl5% 20 L
Sigma-Aldrich 239305
200 L
1,000 L
99%100 mL
Tween 20
Sigma-Aldrich P7949
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NovaSeq 6000 Sequencing System Site Prep Guide
21
NovaSeq Xp 1 · NovaSeq Xp 2-Lane Kit · NovaSeq Xp 4-Lane Kit
· 20021664 · 20021665
NovaSeq Xp 0.5 mL 1.7 mL
NovaSeq Xp 1 · NovaSeq Xp 2-Lane
Manifold Pack · NovaSeq Xp 4-Lane
Manifold Pack
· 20021666 · 20021667
PhiX Control v3 FC-110-3001
NaOH Tween 20
· SPS1 S2
2 · S4 4 ExAmp
NovaSeq Xp
PhiX
X
X PW1 X 18 M X Milli-Q X Super-Q
X
-25^ -15^ 500 mL 20 µL 200 µL 1,000 µL 2^ 8^ * NovaSeq Xp NovaSeq Xp
20021663
* 2 61 cm × 91.4 cm × 25.4 cm24 × 36 × 10
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2019 1
2018 9
SP 300 SP 500
UNC
2018 7 2018 5
2017 9
Compute Engine IP IP
· UPS · UPS · S2 BaseSpace ·
S1 S4 · · NovaSeq 6000 Sequencing System Guide1000000019358 · BaseSpace EU · BaseSpace IP
Windows Windows Compute Engine IP 99%
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NovaSeq 6000 Sequencing System Site Prep Guide
1000000019360 v01
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2017 4
2017 1
Custom Protocol Selector · × × ·
· · · 25^ 80% · 50/60 Hz · · NEMA L6-20P · UPS · UPS
· × × UPS/ · UPS 300 W · UPS 23 cm10
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NovaSeq 6000 Sequencing System Site Prep Guide
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