DeltaPix InSight - Manual and Help User manual

DeltaPix InSight - Manual and Help

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Microscope Camera - COOL09DPX - DeltaPix

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DeltaPix InSight Manual-1

DeltaPix InSight - Manual and Help
USERS MANUAL
v6.7.2
Document processed : 05/07/2022 - 09.57 DeltaPix Support makes no warranties as to the accuracy of the information contained in this document or
its suitability for any usage. The information in this document is subject to change without notice.
Copyright © 2002 - 2022 DeltaPix Support. All rights reserved

DeltaPix InSight - Manual and Help

Table of Contents

Foreword

Part I Introduction

Part II x64 - Limitations

Part III Software Installation

1 System R..e..q..u..i.r.e..m....e..n..t.s.............................................................................................................. 10 2 Sequence...t..o...I.n..s..t.a..l.l..t.h...e...S..o..f.t..w...a..r.e............................................................................................ 10

Part IV Quick System Setup - Wizard

Part V Software Quick Guide

1 System S.e..t..t.i.n..g..s........................................................................................................................ 16
2 Control P..a..n..e..l........................................................................................................................... 16
Language..a..n..d....u..s..e..r..i.n..t.e..r..f.a..c..e...s..t.y..l.e..s..e..l.e..c..t.i.o...n................................................................................................. 16 Options ......................................................................................................................................................... 17
Use HD a.s...i.m...a..g..e...m...e..m...o..r.y........................................................................................................................ 18 Ask for co..r.r.e..c..t..M...a..g..n..i.f.ic..a..t.i.o..n..................................................................................................................... 18 Allow me.m...o.r..y..g..a..r.b..a..g..e...c..o..l.l.e.c..t.i.o..n.............................................................................................................. 18 Save Ima.g..e...Q...u..a..li.t.y...*.*..*.*.*........................................................................................................................... 19 Embed D.P..I..i.n..f.o...w..h..e..n...s..a..v..i.n..g..i.m...a..g..e..s......................................................................................................... 19
Microsoft .W...o..r.d...i.m...a..g..e...q..u..a..li.t.y.............................................................................................................. 19 Allow Cam...e..r.a...O...p..t.i.m...iz..a..t.i.o..n....................................................................................................................... 20 Prefer RG..B...g..a..i.n........................................................................................................................................ 21 Disable h.a..r.d..w..a..r..e..a..c..c..e..l.e..r.a..t.i.o..n..o..f..3..D....g..r.a..p..h..i.c.s........................................................................................... 21 Password.................................................................................................................................................. 21 Monitor s.iz..e............................................................................................................................................... 21 Dongle pr.o..g..r..a..m...m...i.n..g....................................................................................................................................... 21 Dongle pr.o..g..r..a..m...m...i.n..g......D...r.a..g...a..n..d....d..r.o..p........................................................................................................... 22
3 Dock Bar.s.................................................................................................................................. 22
4 Quick Ac.c..e..s..s...T..o..o..l.b...a..r..(.Q...A...T..).................................................................................................. 23
5 The Galle..r.i.e..s............................................................................................................................. 24
6 The Prog.r.e..s..s...B...a..r..................................................................................................................... 25
7 Free Mem..o..r..y...I.n..d..i.c..a..t.o...r............................................................................................................ 25
8 Show Ab.s..o..l.u..t.e...o...r..R...e..l.a..t.i.v..e...m...a..g..n..i.f..i.c..a..t.i.o..n.............................................................................. 25
9 Joystick .c..o..n..t.r..o..l....................................................................................................................... 27

Part VI Camera Control

1 Live Prev.i.e..w............................................................................................................................... 29 2 Camera C..o..n...t.r.o..l........................................................................................................................ 30
Camera c.o..o..l.i.n..g................................................................................................................................................ 30
3 Light ................................................................................................................................... 32

Contents

4 Auto Exp.o..s..u..r..e.......................................................................................................................... 33
5 Colour ................................................................................................................................... 33
Colour Ba..l.a.n...c..e.....G...l.o..b...a.l................................................................................................................................. 33 Colour Ba..l.a.n...c..e.....A...r.e..a..................................................................................................................................... 33 Colour co.r..r.e..c..t.i.o..n............................................................................................................................................. 34
For Infinit.y...X..,..I.n..f.i.n..it.y...X..3..2...a..n..d...t.h..e...D...P...c..a..m...e..r.a..s......................................................................................... 34 For Inven.i.o...a..n..d...I.n..v.e..n..i.o..I.I..C..a..m...e..r..a.s............................................................................................................ 35 For Inven.i.o..I.I.I..C..a..m...e..r.a..s.............................................................................................................................. 35
6 Preview H...i.s..t.o..g..r..a..m.................................................................................................................... 37
7 Using Pro..p...e..r.t.i.e..s...f.r..o..m....a..n...I.m....a..g..e...S..t.o...r.e..d...i.n...a...G...a..l.l.e..r..y............................................................ 37

Part VII Still Image Capture

1 Capture S..t..i.l.l..I.m...a..g..e................................................................................................................... 38
Insert Sca..l.e..B...a..r............................................................................................................................................... 38 Crop Imag..e....................................................................................................................................................... 38 Easy save......................................................................................................................................................... 40 Reset Ima.g..e...C...o..u..n..t..e..r...................................................................................................................................... 41 48 Bits pe.r...P..i.x..e..l..M...o..d..e..................................................................................................................................... 41 Insert Cro.s..s..h..a..i.r...o..v..e..r.l.a..y.................................................................................................................................. 41 Insert Cal.i.b..r.a..t.e..d...G...r..i.d...o..v..e..r.l.a..y......................................................................................................................... 42
2 Capture I.m...a..g..e...U...s..i.n..g...t..h..e...S..n..a..p...s..h..o..t..M...o...d..e............................................................................. 42

3 Save Ima.g..e................................................................................................................................ 43
Image Sav..i.n..g...i.n....a...G..a..l.l.e..r..y............................................................................................................................... 44 Image Sav..i.n..g...b...y..R...i.g..h..t.-..c..l.i.c.k..i.n...g...t.h..e...I.m...a..g..e..................................................................................................... 44 Image Sav..i.n..g...u...s..i.n..g...t.h..e...S...t.a..n..d..a..r.d...M...e..n...u........................................................................................................ 45 Image Sav..i.n..g...b...y..R...i.g..h..t.-..c..l.i.c.k..i.n...g...a...G..a..l.l.e..r..y...................................................................................................... 45 EXIF ......................................................................................................................................................... 45

Part VIII Calibration

1 Defining .t.h..e...o..p...t.i.c..a..l..d..e..v..i.c..e...a..n..d....m...a..g..n..i.f.i.c..a..t..i.o..n..s.................................................................... 49
Make the .c..a..l.i.b..r.a..t.i.o..n....p..r.i.v..a..t.e...o..r...s..h..a..r.e..d........................................................................................................... 49 Add an op..t.i.c..a..l..d..e..v..i.c..e...................................................................................................................................... 50 Add an op..t.i.c..a..l..m...a..g..n..i.f.i.c..a..t.i.o...n.......................................................................................................................... 51 Add a pre.f.e..r.r..e..d...e..x..p..o..s..u..r.e...t..i.m...e...f.o..r..a..n...o...b..j.e..c..t.i.v..e............................................................................................ 51
Relative e..x..p.o..s..u..r.e...................................................................................................................................... 51 Add a DO.F...f.o..r..a..n....o..b..j.e..c..t.i.v..e............................................................................................................................. 53 Adding an...i.n..f.o..r..m...a..t.i.o..n...f.i.l.e............................................................................................................................... 53 Defining c..a..m...e..r.a..-..i.n..t..e..g..r.a..t.e..d...f.o...c..u..s...c..o..n..t.r.o...l.l.e..r..Z..-.c..a..l.i.b..r..a..t.i.o..n.......................................................................... 54
Calibratin.g...t.h..e...f.o..c..u..s..c..o..n..t.r.o..l.l.e..r..a..n..d...d..e..f.in..i.t.i.o..n...i.n...t.h.e...o..p..t.i.c..s...d..e..f.in..i.t.i.o..n...e..d..it.o..r............................................... 56 Defining f.i.e..l.d...c..u..r..v.a..t..u..r.e...c..o..m...p..e..n...s..a.t..i.o..n.......................................................................................................... 61
2 Calibrate..O...n..e...O...b..j.e..c..t.i.v..e........................................................................................................... 63
Calibratio.n...s..l.i.d..e..r............................................................................................................................................. 63 Select the..d..e..v..i.c..e...a..n..d....m...a..g..n..i.f.i.c..a..t.i.o..n...t..o...b..e...c..a..l.i.b..r.a..t.e..d.................................................................................... 64 Draw calib..r..a..t.i.o..n...r..u..l.e..r..................................................................................................................................... 64 Calibrate .t.o...r.e..a..l..w...o..r..l.d...m...e..t.r.i.c..s........................................................................................................................ 64
3 Calibrate..a...C..a..m....e..r.a...a..n..d...M...o...r.e...O...b..j.e..c..t.i.v..e..s............................................................................... 65
4 Calibratin..g...a...m...o...t.o..r.i.z..e..d...z..o..o...m....o..b..j.e..c..t..i.v..e................................................................................ 65
Defining t.h..e...O...b..j.e..c..t.i.v..e...i.n...t.h..e...O...p..t.i.c..s...E...d..i.t.o..r................................................................................................... 65 Associatin..g...t..h..e...D..e..s..c..r.i.p...t.i.o..n...f.i.l.e...................................................................................................................... 66 Defining t.h..e...D...e..p..t.h...o..f..F..i.e..l.d.............................................................................................................................. 67

DeltaPix InSight - Manual and Help

Calibratin.g...t.h..e...O...b..j.e..c..t.i.v..e................................................................................................................................. 68 Motor Zoo..m....-..V...i.r.t.u..a..l..f.i.x..e..d....o..b..j.e..c..t.i.v..e...p..o..s..i.t.i.o..n..s.............................................................................................. 69
5 Calibratio..n...o..v..e..r..v..i.e..w................................................................................................................. 71

6 Calibratio..n...B...a..c..k..u..p...a..n..d....R..e..s..t.o...r.e............................................................................................. 71
Backing u..p...t.h..e...C..a..l.i.b...r.a..t.i.o..n...f..i.l.e.s...................................................................................................................... 72 Restoring..t.h..e...C...a..l.i.b..r.a..t.i.o...n...f.i.l.e..s........................................................................................................................ 74
7 Scale Bar................................................................................................................................... 75

8 Walton an...d...B..e..c..k..e..t..t..g..r.a..t..i.c..u..l.e.................................................................................................. 75

9 Calibratin..g...a...m...o...t.o..r.i.z..e..d...Z...e..i.s..s...M...T..B....z.o...o..m....o..b...j.e..c..t.i.v..e............................................................. 76
Defining t.h..e...m...i.c..r..o..s..c..o..p..e................................................................................................................................. 77 Defining t.h..e...m...i.c..r..o..s..c..o..p..e...m...a..g..n..i.f.i.c..a..t.i.o...n......................................................................................................... 78 Calibratin.g...t.h..e...m....o..t.o..r.i.z..e..d...o..b...j.e.c..t..i.v..e............................................................................................................... 79 Controllin.g....t.h..e...m...i.c..r.o..s..c..o..p...e............................................................................................................................ 80
10 Sharing C..a..l.i.b...r.a..t.i.o..n...s................................................................................................................ 80
For the us..e..r..w...i.t.h...a...c..o..m...p...l.e.t..e...c..a.l.i.b...r.a..t.i.o..n........................................................................................................ 81 Copy the .c..a.l.i.b...r.a..t.i.o..n...t..o...a..n..o..t.h..e..r...u..s..e..r............................................................................................................. 82

Part IX The Home Tab

1 The Editi.n..g...T..o..o...l.s..................................................................................................................... 83
2 The Form..a..t.t.i.n..g....T..o..o..l.s............................................................................................................... 83
3 Reporting................................................................................................................................... 83
Realize ......................................................................................................................................................... 84 Excel Rep..o..r.t.................................................................................................................................................... 84
Sample E..x.c..e..l..R..e..p..o..r.t..t.e..m...p..l.a..t.e.................................................................................................................. 85 Sample E..x.c..e..l..R..e..p..o..r.t..o..u..t.p..u..t..................................................................................................................... 87 PDF Repo..r.t...................................................................................................................................................... 89 Comma S.e..p..e..r.a..t..e.d....V..a..l.u..e...R...e..p..o..r.t.................................................................................................................... 89 Exporting..C...S..V...d...a..t.a...t.o...M...i.c..r.o..s..o...f.t..E..x..c..e..l......................................................................................................... 89 The Com.m...a...S..e..p..a..r.a..t.e..d...V..a..l.u..e...f.i.le.............................................................................................................. 90 Import to.M...i.c..r.o..s..o..f.t..E..x..c.e..l..2..0..0..7.................................................................................................................. 91
4 The Wind..o..w....t.o..o..l.s..................................................................................................................... 93

Part X The View tab

1 Image Til.e.................................................................................................................................. 93

Part XI The Measurement and Annotation

tab

1 Annotatio..n................................................................................................................................. 94

2 Measurem...e..n..t............................................................................................................................ 95
Polygon a.n...d...F..r.e..e..h..a..n..d....r.e..s..u..l.t..o..p..t..i.o..n..s............................................................................................................ 95 Circle me.a..s.u...r.e..m...e..n..t..r..e..s..u..l.t..o..p..t.i.o...n..s................................................................................................................ 95 Flexible p.o..s..i.t.i.o..n....f.o..r..m...e..a..s..u..r..e..m...e..n..t..r..e.s..u...l.t..................................................................................................... 96
3 Save the .A...n..n..o..t.a..t..i.o..n..s...a..n..d....M...e..a..s..u..r.e..m...e..n...t.s............................................................................. 96
Save and .O...p..e..n...t.h..e...A...n..n..o..t.a..t..i.o..n..s...o..n..l.y............................................................................................................. 96 Save the A...n..n..o..t.a..t.i.o..n...s...a.n...d...M...e..a..s..u..r.e..m...e..n..t..s..i.n....S..e..p..e..r.a..t..e..F...i.l.e..s........................................................................ 97 Save the A...n..n..o..t.a..t.i.o..n...s...a.n...d...M...e..a..s..u..r.e..m...e..n..t..s..i.n....t.h..e...S..a..m...e...F..i.l.e.......................................................................... 97

Part XII The Still Image Tab

Contents

1 Undo ................................................................................................................................... 97 2 Remap In..t.e..n..s..i.t.y........................................................................................................................ 98 3 Fluoresce..n...c..e............................................................................................................................ 99 4 Deconvo...l.u..t.i.o..n......L...u..c..y..-.R...i.c..h..a..r.d..s..o...n...a..l.g..o..r..i.t.h..m....................................................................... 100

Part XIII The Image Extender Tab

101

1 Manual .E..x..t.e..n...d..e..d...F..o..c..u...s........................................................................................................ 101 2 Extende.d....E..x..p..o..s..u...r.e................................................................................................................ 102
Contrast..p..o..s..t..p...r.o..c..e..s..s..i.n..g.............................................................................................................................. 103
3 Manual .E..x..t.e..n...d..e..d...F..i.e..l.d........................................................................................................... 103

Part XIV The Counting Tab [from v6.1.18]

105

1 Worksp.a..c..e.............................................................................................................................. 106
2 Phase S.e..g..m....e..n..t.a..t.i.o...n.............................................................................................................. 107
Pick obje..c..t..c..o..l.o..r............................................................................................................................................ 107 Phase D.e.f..i.n..i.t.i.o..n...O...v..e..r.l.a..p............................................................................................................................... 108
3 Morpho.l.o..g..y...p..r..e..p..r.o..c..e..s..s..i.n...g................................................................................................... 108
4 Area Fea..t..u..r.e............................................................................................................................ 108
5 M1 Feat.u..r.e............................................................................................................................... 108
6 Ferret F.e..a..t.u..r..e......................................................................................................................... 109
7 Countin.g.................................................................................................................................. 110
Settings......................................................................................................................................................... 110 Use sam..e...f.e..a..t.u..r.e..s.................................................................................................................................. 111
Counting...O...p..t.i.o..n..s........................................................................................................................................... 112 Create T..a..b..l.e........................................................................................................................................... 112 Insert C.o.u..n..t..L..a..b..e..l.................................................................................................................................. 113 Create N..e..w....I.m...a..g..e.................................................................................................................................. 113 Object in..d..e..x...la..b..e..l................................................................................................................................... 113
8 Reportin..g................................................................................................................................. 113
Excel ......................................................................................................................................................... 113 Template...t.a..g..s................................................................................................................................................. 114 PDF ......................................................................................................................................................... 114 Excel pro...c.e..s..s..i.n..g.......e..x.a..m....p..l.e...c..a..l.c..u..l.a..t.i.n..g...t.h..e...P...h..a..s..e..%.................................................................................. 116
9 Manual .C..o...u..n..t.i.n..g..................................................................................................................... 116

Part XV The System Automation Tab

117

1 Stage A.u..t.o..m...a..t..i.o..n................................................................................................................... 117
Selecting...a..n..d....C..o..n..f..i.g..u..r.i.n..g.....t.h..e...M...o..t.o..r...C..o..n...t.r.o..l.l.e..r........................................................................................ 118 Prior Co.n..t.r.o..l.l.e..r....................................................................................................................................... 118 Zeiss M.T..B............................................................................................................................................... 120 Ludl MA.C...5..0.0..0../.M...A...C..6..0..0..0...C..o..n..t.r..o.l.l.e..r....................................................................................................... 120 Märzhau..s..e.r...T..A..N...G..O................................................................................................................................. 122 DeltaSte..p..4.............................................................................................................................................. 124 Mitutoyo..-...M..F........................................................................................................................................... 125 Mitutoyo.-...I.n..s.e..r.t..S...c..a..le...B...a..r............................................................................................................... 125 Mitutoyo.-...E..X..I.F...I.n..f.o..r.m...a..t.i.o..n............................................................................................................... 126
Making t.h..e...m...o..t..o..r..s..p..e..e..d...r..e..l.a..t.i.v..e...t.o...t.h..e...M...a..g..n..f..i.c..a.t..i.o..n................................................................................. 126

DeltaPix InSight - Manual and Help

Verifying...t.h..e...X...a..n..d...Y....m...o..v..e..m...e..n..t..s..c..h..e..m....e.................................................................................................... 127
2 Automa.t.i.c...E..x..t.e..n...d..e..d...F..i.e..l.d...................................................................................................... 128
Automat.i.c...E..x..t.e..n..d..e..d....F..i.e..l.d...w...i.t.h...E..x..t.e..n..d...e..d...F..o..c..u..s...a..n..d...E...x..t.e..n..d..e..d...E...x..p..o..s..u..r.e................................................ 129
3 Automa.t.i.c...X..Y...Z...A..c..q...u..i.s..i.t.i.o..n.................................................................................................... 129
Acquisiti.o...n...O..p...t.i.o..n..s........................................................................................................................................ 130 Define th..e...t.a..r.g..e..t..i.m....a.g...e...d..i.r.e..c..t.o..r..y................................................................................................................. 131 The posi.t.i.o..n....t.a..b..l.e.......................................................................................................................................... 131 Defining..I.m...a..g..e...P..o..s..i.t..i.o..n...m...a..c..r.o..s...i.n....t.h..e...`.C..o...m...m...e..n..t.'..f.i.e..l.d............................................................................. 131 Defining..P..o..i.n..t...T..r.a..n..s..f.o..r..m...a..t.i.o..n...m....a.c..r..o..s...i.n...t.h..e...`.C...o..m...m...e..n..t..'..f.i.e..l.d.................................................................. 133 Table Ma..c..r.o...p..r..o..g..r.a..m....m...i.n..g............................................................................................................................ 134
Table M.a..c..r.o...P..r.o..g..r.a..m...m...i.n..g...-..E..x..a..m...p..l.e...c..o.d..e............................................................................................. 139
4 Focus a.u..t.o...m...a..t.i.o..n................................................................................................................... 140
Selecting...a..n..d....C..o..n..f..i.g..u..r.i.n..g.....t.h..e...M...o..t.o..r...C..o..n...t.r.o..l.l.e..r........................................................................................ 141 Prior Co.n..t.r.o..l.l.e..r....................................................................................................................................... 141 Zeiss M.T..B............................................................................................................................................... 142 Ludl MA.C...5..0.0..0../.M...A...C..6..0..0..0...C..o..n..t.r..o.l.l.e..r....................................................................................................... 143 Märzhau..s..e.r...T..A..N...G..O................................................................................................................................. 146 DeltaSte..p..4.............................................................................................................................................. 147 Mitutoyo..-...M..F........................................................................................................................................... 148 Mitutoyo.-...I.n..s.e..r.t..S...c..a..le...B...a..r............................................................................................................... 148 Mitutoyo.-...E..X..I.F...I.n..f.o..r.m...a..t.i.o..n............................................................................................................... 149 ErgoFoc.u..s............................................................................................................................................... 150 Stand alo..n..e...m...o..d..e...-..p..a..r.a..m...e..t.e..r.s........................................................................................................ 152 ErgoFoc.u.s...f.i.r.m...w...a..r.e...v.e..r..s.i.o..n..s............................................................................................................ 153 Camera..w..i.t.h...i.n..t.e..g..r.a..t.e..d...f.o.c..u..s...c..o..n..t.r.o..l..................................................................................................... 153
Automat.i.c...E..x..t.e..n..d..e..d....F..o..c..u..s........................................................................................................................... 153 Setting t.h..e...N...u..m...b.e..r...o..f..Z..-.s..li.c..e..s...t.o...b..e...a..c.q..u..i.r.e..d.......................................................................................... 154 Using th.e...D...O..F...f.r..o..m....t.h..e..O...p..t.i.c..s...E..d..i.t.o..r.................................................................................................... 154
Automat.i.c...E..x..t.e..n..d..e..d....F..o..c..u..s...w...i.t.h...E..x..t.e..n...d..e..d...E..x..p..o..s..u...r.e................................................................................. 155 Auto Foc..u..s...[.f.r..o..m....v..6...6...7..].............................................................................................................................. 155
One reg.i.o..n...A..u..t.o...F..o..c..u..s........................................................................................................................... 167 Global ce..n..t.e..r..w...e..ig..h..t.e..d...A...u.t.o...F...o..c.u..s................................................................................................... 168 One Reg.i.o..n...A..u..t.o...F..o..c..u..s.................................................................................................................... 168
Two reg.i.o..n..A...u..t.o...F..o..c..u..s........................................................................................................................... 169 Making t.h..e...m...o..t..o..r..s..p..e..e..d...r..e..l.a..t.i.v..e...t.o...t.h..e...D...O..F................................................................................................ 171
5 Zoom A.u..t.o..m...a..t..i.o..n................................................................................................................... 172
Zeiss MT..B....................................................................................................................................................... 172 DeltaSte.p..4...................................................................................................................................................... 173

Part XVI Accessories Tab

174

1 Zeiss m.i.c..r.o..s..c..o...p..e...c..o..n..t.r..o..l.s.................................................................................................... 174 2 Mettler T..o...l.e..d..o...H...o..t..S...t.a..g..e...H...S..8..2............................................................................................. 174 3 PixLight...R..i.n..g...l.i.g..h..t..(.V...a..l.i.d...f.r..o..m....I.n..S...i.g..h..t..6...4..).......................................................................... 176 4 Objectiv..e...c..h..a..n..g..e...s..c..a..n..n...i.n..g................................................................................................... 178
Example.:..A...s..k..a..n..i.a...6..Z..................................................................................................................................... 179

Part XVII Image Compare

180

Part XVIII Topography [from v6.4.0]

186

1 File ................................................................................................................................... 187

Contents

Open ......................................................................................................................................................... 187 Save ......................................................................................................................................................... 188
2 Topogra..p..h..y............................................................................................................................. 190
Manual T..o..p..o..g...r.a..p..h..y....................................................................................................................................... 190 Automat.i.c...m...o..t.o..r..i.z.e..d....T..o..p..o..g..r..a..p..h..y................................................................................................................ 191 Use Z ra.n..g..e...f.r.o..m.....p..r.e..v..i.o..u..s...t.o...p..o..g..r.a..p...h..y....................................................................................................... 191 Paramet.e.r..s..................................................................................................................................................... 192
3 Compen..s..a..t.i.o..n......................................................................................................................... 192

4 3D View................................................................................................................................... 193

5 3D Meas..u..i.r..e..m...e..n..t................................................................................................................... 194
3D Roug.h..n..e..s..s................................................................................................................................................ 196 Abbot F.ir.e..s..t.o..n..e....................................................................................................................................... 197
6 2D View................................................................................................................................... 197

7 2D Meas..u..r..e..m...e..n..t.................................................................................................................... 198
2D Roug.h..n..e..s..s................................................................................................................................................ 199 2D Profil.e...M...e..a..s..u..r.e..m....e..n..t.s.............................................................................................................................. 200
8 Reportin..g................................................................................................................................. 203
Template......................................................................................................................................................... 204

Part XIX Video recording [from v6.2.9]

204

1 Recordi.n..g...f.r..a..m...e...r.a..t..e............................................................................................................. 205 2 Playbac.k...f.r.a..m....e...r.a..t.e................................................................................................................ 205 3 Recordi.n..g...t.i.m....e....................................................................................................................... 206 4 Informa.t.i.o..n.............................................................................................................................. 206 5 Recordi.n..g................................................................................................................................ 206

Part XX Shortcuts and special Keys

207

1 Special .s..t.a..r.t..-.u..p...m....o..d..i.f.i.e..r...k..e..y..s.............................................................................................. 209 2 Special .s..h..u..t..-.d..o..w...n...m...o...d..i.f.i.e..r...k..e..y.s.......................................................................................... 210 3 Motor sp...e..e..d...m...o..d..i.f..i.e..r..k..e..y..s.................................................................................................... 210

Part XXI FAQ - Frequently Asked Questions

211

1 Softwar.e...f.r.e..e..z..e...c..h..e..c..k..l.i.s..t...................................................................................................... 211 2 Installin.g...P...r.i.o..r...s..o..f.t.w...a..r.e......................................................................................................... 212 3 Underst.a..n..d..i.n...g...m...a..g..n..i.f..i.c..a..t.i.o..n................................................................................................ 212 4 Get rid o..f..o...l.d...I.n..v..e..n..i.o...3..S.../.5..S...d..r..i.v..e..r.s...................................................................................... 212 5 Install d.r.i.v..e..r...f.o..r...F..o..r..I.n...v..e..n..i.o..I.I.I.e..b...C...a..m...e..r..a..s.......................................................................... 213 6 Disable .I.n..t..e..r.n..e..t..c..o...n..n..e..c..t.i.v..i.t.y................................................................................................. 216 7 Frame ra..t..e...d..r.o...p..s.................................................................................................................... 217 8 Fix an A.n..n...o..y..i.n..g...W....i.n..d..o..w...s...U...S..B....P..o..w...e..r...P..r.o...b..l.e..m.................................................................. 217 9 ATEN U.S..B....t.o...R...S..2..3..2...c..o..n..v...e..r.t.e..r...d..r.i.v..e..r.................................................................................. 218 10 USB Cab...l.e..s............................................................................................................................. 219 11 Graphic.s...p..e..r..f.o..r.m....a..n..c..e........................................................................................................... 219

DeltaPix InSight - Manual and Help

12 A gener.i.c...e..r.r.o...r..o..c..c..u..r..r.e..d...i.n....G..D...I.+.......................................................................................... 220

Part XXII Appendix

220

1 Backup.-...R..e..s..t.o...r.e...-..C...l.o..n..i.n..g.................................................................................................... 221 2 External..D...r.i.v...e...f.o..r..S...e..t.t.i.n..g...s.................................................................................................... 225
Export S.e..t.t.i.n...g..s....t.o...E..x..t.e..r..n..a..l..S..e..t.t..i.n..g..s...D..r..i.v..e...-..M...e..t.h..o..d...1............................................................................... 226 Export S.e..t.t.i.n...g..s....t.o...E..x..t.e..r..n..a..l..S..e..t.t..i.n..g..s...D..r..i.v..e...-..M...e..t.h..o..d...2............................................................................... 227 Import S.e..t.t.i.n..g...s....f.r.o..m....E...x..t.e..r.n..a..l..S...e..t.t.i.n..g..s...D...r.i.v..e............................................................................................. 229
3 Installin.g...a..n..d....c..o..n..n..e..c..t.i.n...g...t.o...P...r.i.o..r...(.O...p..t.i.S...c..a..n..I.I.).................................................................. 232
Installati.o..n...................................................................................................................................................... 232 64bit ope..r.a..t..i.n..g...s..y..s..t.e..m................................................................................................................................... 232 Prior Op.t.i.S..c..a..n..I.I..Z...-.m...o..t.o..r...o..p..e..r.a..t.i.o...n.............................................................................................................. 232
4 TWAIN ................................................................................................................................... 233 5 DirectX .(.D...i.r.e..c..t.S...h..o..w...)............................................................................................................. 233 6 Matlab ................................................................................................................................... 233 7 Installin.g...a..n..d....c..o..n..n..e..c..t.i.n...g...t.o...Z...e..i.s..s...M...T..B...2..0..1..1...................................................................... 234
Using an..o..t.h..e..r..v..e..r..s..i.o..n...o..f..M...T..B...2..0..1..1...t.h..a..n...t.h..e...d..e..f..a.u...l.t..M...T..B..2..0..1..1...v..e..r.s..i.o...n..................................................... 234 Optics E.d..i.t.o..r...a..n..d...c..a..l.i.b..r.a..t..i.o..n...h..i.n..t.s............................................................................................................... 237
8 Märzhau..s..e..r..T...A..N...G...O................................................................................................................ 238 9 Mitutoyo...M...F....-..C...o..m...m...u..n...i.c..a..t.i.o..n...S...e..t.u..p................................................................................... 239 10 Custom..C..a..m....e..r.a...C...o..n..f.i.g..u...r.a..t.i.o..n.............................................................................................. 240 11 Artray A.R...T..C...A..M...-.1..3..0..X...Q...E..-.W....O...M....C..a..m...e..r..a............................................................................... 242 12 Artray A.R...T..C...A..M...-.9..9..0...S...W...I.R...T..E...C...c..a..m...e..r..a................................................................................ 243
Artray AR...T..C..A...M...-.9..9..0...S..W...I.R...T..E..C....-..D...r.i.v..e..r..i.n..s..t.a..l.l.a..t.i.o...n..................................................................................... 243 Artray AR...T..C..A...M...-.9..9..0...S..W...I.R...T..E..C....-..B...a.s..i.c...s..e..t.u...p............................................................................................... 246
13 Allied Vi.s..i.o...n...-..G...O...L..D..E...Y..E........................................................................................................ 249 14 Conneti.n..g...H...4..K..W....S..D...0..8..D..P...X...b..y...W....i.F..i..................................................................................... 253 15 TEKLEA..D...S...c..a..n..n..e..r................................................................................................................. 254
TEK_E1_..C..C...D.................................................................................................................................................. 256 TEK_E2_..U..S...B.................................................................................................................................................. 256

Index

257

1 Introduction

Introduction 9

DeltaPix InSight is modern and easy to use software. It offers the best interface to DeltaPix cameras and makes advanced image acquisition easier than ever.
However, if you have problems installing or using the software or with general camera operation, you can e-mail our technical support group at support@deltapix.dk or contact your local dealer or
distributor to get further direct support. Please visit our homepage at www.deltapix.dk for software updates or other improvements for your
DeltaPix camera and accessories.
v6.7.2 - Document processed : 05/07/2022 - 09.57

DeltaPix InSight - Manual and Help

2 x64 - Limitations
DeltaPix InSight (from v.6.0.0) is 64bit only. This means, that it cannot be installed on 32bit computers.
Some cameras are no longer supported: DP 200 Invenio 1D Invenio 3D Invenio 5D
Also the MicroSMC stepper motor controller is discontinued.

3 Software Installation

3.1 System Requirements
Windows 7, Windows 8 or Windows 10 - 64 bit versions. A high resolution monitor is recommended, HD (1920x1080) or better. A fast CPU (like a Intel i5 or i7) A modern and fast graphics processor. Shared video memory is not recommended. At least 8 GB of RAM is recommended. At least 25 GB of free disk space USB 2.0 or 3.0 ports - it is not recommended to use USB hubs!

3.2 Sequence to Install the Software
Detach any DeltaPix camera and dongle from your PC. Use the DeltaPix Installation Manual to install the new software package from your CD or from www.deltapix.dk. Attach the dongle (Note: Most DeltaPix InSight versions require a dongle to run). If it is the first time the dongle is attached you should see a 'Found New Hardware' sequence. Wait for the sequence to complete. Later dongle attachments will issue the Windows '' `hardware attached' sound. Attach the camera. If it is the first time the camera is attached you should see a 'Found New Hardware' sequence. Wait for the sequence to complete. Later camera attachments will issue the Windows 'hardware attached' sound.

Start the DeltaPix InSight software.

Software Installation 11

4 Quick System Setup - Wizard
The `Quick System Setup' tool will automatically launch on the first run after the software is installed. It allows for super fast configuration of all the motor parameters, XY, Focus and optional Zoom control all just by selecting the system type (like e.g. `AB 70S') and the communication COM port. The factory calibration files are now also loaded via this tool. The list of supported systems will be updated as needed. The `Quick System Setup' can be launched manually at any time by shortcut Ctrl-Shift-W.
The wizard launches the first time after installation of a new version.
If you haven't made any changes to attached hardware and if you are happy with the current setup click `cancel'
To continue the Quick System Setup, click `Next'.

DeltaPix InSight - Manual and Help

If you have a backup, cloning is the easiest way to set up your DeltaPix InSight.

If you don't have a backup, skip this step by 'Next'.

See also: Backup - Restore - Cloning
Select your system.
If your hardware is not listed, click `Cancel'.

DeltaPix InSight can check for new software releases and other news from DeltaPix.
On some computers with tight security settings, this may cause troubles.
If in doubt contact your IT department, or simply disable the DeltaPix InSight internet connectivity.

Quick System Setup - Wizard 13

Some systems communicate via a COM port now select the associated COM port, and click `Next'.

DeltaPix InSight - Manual and Help

If you have calibration files, check the check box, then click `Next'.

All done...
When everything is set up, DeltaPix InSight may have to restart to make the settings take effect.
You will have to manually launch the program afterwards.

Software Quick Guide 15
5 Software Quick Guide
This is an overview of the GUI with outlines of the basics:
DeltaPix InSight has a modern and intuitive user interface based on the ribbon band style known from Microsoft Office. Version 2007 and 2010 of the Microsoft Office and the Visual Studio 2010 style is supported by the software. Functionalities are grouped logically and the program is easy to use. Features are:
Camera panel for fine-tuning of all camera parameters e.g. exposure, gain, and more Device panel for selection of camera and optics Panorama view for easy image manoeuvring in zoomed live and still images Multiple image galleries with thumb view Easy storage of images
© 2022 DeltaPix Support

DeltaPix InSight - Manual and Help

5.1 System Settings

The upper-left button in the GUI opens a `standard' office dropdown menu, allowing: File open File save Printing E-mailing Online manual (this manual, latest version)
The System Maintenance button opens functions used seldom, e.g.: Optics definition editor Control Panel On line list of known software issues About

5.2 Control Panel
The control panel hosts general application settings.
5.2.1 Language and user interface style selection
Language can easily be switched. More than ten languages are supported.

InSight can appear in a variety of modern styles, select your preference among Office2007, Offife2010 or `Visual Studio' styles.

Software Quick Guide 17

5.2.2 Options
The option panel allows setting of lower level options.

DeltaPix InSight - Manual and Help

5.2.2.1

Use HD as image memory
It saves RAM to use this option - it also however slows some image operations slightly. It is highly recommended to use this option if a fast HD is available.

5.2.2.2

Ask for correct Magnification
When using a system with manual magnification change - such as a objective carousel without feedback, this option is recommended. It will prompt "Is correct magnification selected" in the software. This ensures that calibration is always correct, and measurements are reliable.

5.2.2.3 Allow memory garbage collection

This option often makes the use of system memory more efficient. On some special computers, especially Windows XP computers, this option may cause problems.

Software Quick Guide 19

5.2.2.4

Save Image Quality *****
The global image save quality sets the image compression factor. The image compression factor (2-100) is shown in the parenthesis.

The quality is selected by clicking on a star. It is strongly recommended to use the 5 star setting (compression lowest = 2)
5.2.2.5 Embed DPI info when saving images. If captured images are to be imported in e.g. Microsoft Word, high magnification images (i.e. a huge DPI), will appear very small. Uncheck this to avoid true embedded DPI.
5.2.2.5.1 Microsoft Word image quality
Word tries to reduce the resolution to file footprint smaller. You may need to tell it not do to that. Go to File / Options / Advanced / Image Size and Quality. Check the box that reads Do Not Compress images in file. Here's how it looks:

DeltaPix InSight - Manual and Help

5.2.2.6

Allow Camera Optimization
Some types of cameras - mostly CMOS cameras may benefit from this pixel optimization. Poor pixels are detected, and will be corrected/compensated to achieve the optimum still image quality.

Software Quick Guide 21

5.2.2.7

Prefer RGB gain
The option sets the preferred way of adjusting the image colors. By clicking "Prefer RGB gain" the control is done by RGB sliders, otherwise by Temp/Tint sliders.

Temp/Tint is the default, although not all supported cameras support this. Cameras that does not support Temp/Tint will automatically revert to RGB control.

Changing this value may require the camera to reconnect, or to restart the DeltaPix InSight

5.2.2.8

Disable hardware acceleration of 3D graphics
It is possible to disable hardware acceleration of 3D rendering. This will slow down all 3D operations. It is highly recommended to always use hardware acceleration of 3D graphics.

NOTE: Some Intel GPU's fail to work with DeltaPix InSight and hardware acceleration - in these rare cases it may become necessary to disable the hardware acceleration.

5.2.2.9

Password
A password can be entered to protect vital settings like the system calibration and motor controller configurations. This will in many cases protect against unwanted tampering.

5.2.2.10 Monitor size
What exactly is magnification? A basic definition of magnification is the ratio of the size of a specific feature of an object or sample as seen in an image produced by an optical system to the actual size of the feature on the object itself. In digital imaging this can be defined as the ratio between the size of the object as shown on the monitor and the actual physical size of the object.
So in order to display the correct magnification, the size of the monitor also must be known.

5.2.3 Dongle programming

A DeltaPix dongle can be updated with new features. Load the dongle file, and push the program button.

DeltaPix InSight - Manual and Help

5.2.4 Dongle programming Drag and drop.

Another and easy way to reprogram a dongle is simply to drag the dongle programming file into the DeltaPix InSight' program icon.

5.3 Dock Bars

The program uses the concept of document docking. This allows the dock bars to be hidden or showed at fixed locations.

To hide the dock bar click the To view, move the cursor to the To show the dock bar click the

pin.

tab.

pin again.

It is also possible to move and rearrange the dock bars to other locations in the GUI.
To move a dock bar on top of another dock bar simply drag the dock bar and place it on the top spot of the cross. This is shown in the figure below:

Software Quick Guide 23

Dock to left:

Dock to bottom

Dock to right:

Docking may seem complicated and confusing it is however a nice feature. If it ends up in a mess you can always select Reset user interface layout in the System Maintenance menu. Saving a layout is also done from the System Maintenance menu.
5.4 Quick Access Toolbar (QAT)
The Quick Access Toolbar is a customizable toolbar which contains a set of commands, that are independent of the tab displayed currently. You can move the Quick Access Toolbar from one of the two possible locations, and you can add buttons that represent commands to the Quick Access Toolbar. The QAT can be minimized to save screen area. The QAT can also be placed below the ribbon bar.
© 2022 DeltaPix Support

DeltaPix InSight - Manual and Help

A button or other control can be mirrored to the QAT. Right-click the command and then click Add to Quick Access Toolbar in the shortcut menu. In this example it is shown how to add one of the motor control arrow buttons to the QAT.

This example shows:
A minimized ribbon.
The QAT placed below the ribbon
Two arrow (Focus Motor up/ down) added to the QAT.
5.5 The Galleries
InSight has two image galleries. Images can be dragged from the the galleries to the image area to open an image. Images can be dragged and dropped in between the galleries. Images can also be dragged to the `Extended Field' editor. Images stored in the gallery can easily have their properties copied back to a camera see section `Using properties from an image stored in a gallery'. NOTE: Loading thumbnails requires computational resources, and may take some time. To prevent unintended motor control, all motor control is disabled while thumbnails are loading A notification will show this state.

5.6 The Progress Bar
For lengthy operations, the progress bar shows the progress of the current operation.

Software Quick Guide 25

5.7 Free Memory Indicator
Some operations require a lot of free (and unfragmented) memory. Usually the memory indicator is not visible which indicates that there is plenty of free memory. A green indicator shows the remaining free memory. Yellow or red indicators also show free memory, but indicate that InSight is running short of memory.
5.8 Show Absolute or Relative magnification
If in `Relative Magnification Mode' the slider text shows the digital zoom percentage.
Click the small magnifier button to switch to `Absolute Magnification Mode'.

If in `Absolute Magnification Mode' the slider text shows the magnification value. Click the small magnifier button to switch to
© 2022 DeltaPix Support

DeltaPix InSight - Manual and Help

`Relative Magnification Mode'.

The magnification depends on the monitor used for display the larger monitor, the larger magnification.
The monitor size defaults to 27" but can be changed in the control panel.

The magnification is calculated as: Magnification = DigitalZoom x ImageDPI / MonitorDiagonalDPI If the magnification shown is 1 then an image of (approximately) the camera sensor size is displayed.
NOTE: The objective must be correctly calibrated to have the correct magnification readout. NOTE: Depending on the resolution setup of your monitor, the pixels on your monitor are not necessarily square in this case the magnification shown is only approximate.
© 2022 DeltaPix Support

5.9 Joystick control
If your software supports joystick control - then in the status line there a small joystick button is shown. The X, Y, F indicates that the 3 axis joystick controls X and Y position and Focus.
Alternatively X, Y and Zoom can be controlled then the text will be X, Y, Z.
Double click the button to configure the (optional) 6 buttons on the joystick.
A panel will pop up each button function can be selected.

Software Quick Guide 27

DeltaPix InSight - Manual and Help

An example of setting the function for button 5.

When mouse hovering the button a balloon will show the current button configuration. Most standard HID joysticks will work. A recommended joystick is RS
© 2022 DeltaPix Support

Software Quick Guide 29
6 Camera Control
6.1 Live Preview
With DeltaPix InSight it is possible to connect multiple cameras at the same time, and switch from one camera to another. All DeltaPix cameras are supported. Other cameras with a DirectShow interface are also supported. However, this requires the DirectShow feature to be enabled in the dongle. Selecting the camera is done in a drop down menu as shown here.
© 2022 DeltaPix Support

DeltaPix InSight - Manual and Help

6.2 Camera Control

Use the adjustment tools to adjust the live preview to the desired quality. The settings drop down allows fine-tuning of all camera parameters.
Note that the options shown depend on the camera type. Not all cameras have all types of controls.

6.2.1

Camera cooling
If the camera supports sensor cooling - then the properties drop down will allow setting of a target temperature, and optionally selecting fan on/off

This is a description of the DeltaPix COOL07DPX, COOL09DPX and COOL20DPX camera cooling user interface in DeltaPix InSight.

In the Settings drop down, find the new properties related to the TEC cooling.
Camera cooling can be set on/off by the "Camera Cooling" check-box. Camera fan can be set on/off by the "Fan" checkbox. The numeric text box defines the target temperature for the TEC cooling.
Please note that the cooling cannot be set on if the "Fan" is off.

Camera Control 31
In the bottom status bar, the cooling status is show. This show the actual temperature. Hint: The proprietary "convert gain to exposure" feature can be used with these cameras. Use high gain in the preview to have fast live preview and convert this to exposure time when capturing still images. Please be aware that when using very high gain, this may convert to very long exposure time. Example if the gain is 100x and the exposure is 500ms, then the exposure time will be 50secs. On top of this ~3 images are acquired so the overall
© 2022 DeltaPix Support

DeltaPix InSight - Manual and Help

acquisition may become long.

Cameras are good quality, with very few hot pixels. Anyway, if any occur, then the "Auto Fix Hotspots" can be used.

6.3 Light
To avoid flickering and horizontal lines when using older AC light sources, set the Light source frequency to the frequency of the light source; 50 or 60 Hz. Using this option will limit the lowest possible exposure time to 1/100 or 1/120 second. Hence, in case that the light level is too high, the light level must be decreased on the light source.

6.4 Auto Exposure
For automatic light adaption, turn on the Auto Exposure. Using this function will turn off the light source synchronization, which may result in flickering light or horizontal grey lines on the
live video (see 4.3).
6.5 Colour
6.5.1 Colour Balance Global
For white/grey balance adjustment on multicoloured preview, use Colour balance Global. To optimize the colour balance, remove any colour objects. An all-grey image makes the best balance.
6.5.2 Colour Balance Area
For preview with very limited areas of white/ gray, use Colour balance Area.
...and point with the cursor at the white/gray area, then click once:

Camera Control 33

DeltaPix InSight - Manual and Help

..1 2 Colour Balance Manual
For manual colour adjustments, use Colour gain:

6.5.3 Colour correction
6.5.3.1 For Infinity X, Infinity X32 and the DP cameras Select the corresponding light source. If the light source is unknown, then experimentation with the functionality can be done.
The colour temperature can be fine tuned using the slider.

6.5.3.2 For Invenio and InvenioII Cameras Select the corresponding light source. If the light source is unknown, then experimentation with the options can be done.
The colour temperature can be fine tuned using the slider.

Camera Control 35

6.5.3.3

For InvenioIII Cameras
If the automatic white balance function fails to give perfect colors, the manual controls can be used for fine tuning.

The InvenioIII cameras comprises two methods of adjusting the colors: The preferred method is selected in the control panel-.

Temp/Tint adjustment Temp and Tint are independent, the temperature slider affects the color temperature, which is effectively the blue-yellow axis, while the tint affects the green-magenta axis. Changing the value of one affects the appearance of the other, hence it makes a difference whether you change the tint value first and then move on to the temperature, or the other way around.

DeltaPix InSight - Manual and Help

Color balance corrections will be done primarily with the Color Temp slider, with minor adjustments to the Tint slider.
RGB adjustment In this mode the colors are adjusted individually.

Camera Control 37
6.6 Preview Histogram
The preview histogram is a tool to ease the camera parameter setting. The histogram shows the distribution of red, green and blue in the preview image.
6.7 Using Properties from an Image Stored in a Gallery
When an image is stored in a gallery, a file containing all the camera properties used when the image was captured is also stored. This is a unique feature, and makes it easy to revert to the settings used when the image was captured. To copy the camera settings from an image to a camera, right click the image and click Copy image settings to camera. Note:
This feature works only from within the gallery. The settings are stored in a separate file. The feature only works with the same camera type that was used for capturing the image
7 Still Image Capture
© 2022 DeltaPix Support

DeltaPix InSight - Manual and Help

7.1 Capture Still Image

Select the desired format and image type. In most cases the capture format can be selected different from the preview format.
Note that the capture options vary depending on the camera type.

Then click on Capture image to capture an image.

7.1.1 Insert Scale Bar
A calibration bar can be automatically inserted on captured images. Note that this requires calibration.
7.1.2 Crop Image Crop to Preview Region It is possible to capture only a part of an image.
In the Capture settings, check the `Crop to

Preview Region'

Still Image Capture 39

Zoom in on the part of the image you would like to acquire then capture the image.

Crop to Square
This option will crop captured images to be square 1:1 format. The live preview image will not be affected.
© 2022 DeltaPix Support

DeltaPix InSight - Manual and Help

7.1.3 Easy save

The work flow can in some cases be optimized by using the Easy save. This automates the process of naming and saving images images are simply named, saved and optionally closed automatically.

Changing the easy save file name also effects the general image naming, even when easy save is disabled.

Still Image Capture 41
7.1.4 Reset Image Counter
The sequential numbering of images can be reset, or set to a specic value
Example: 1320 will be used as the next image counter index.
7.1.5 48 Bits per Pixel Mode
Some CCD camera models support 10, 12 or 14 bits per raw pixel. The common colour format for these cameras is RGB48.
Please note that RBG48 images can only be saved in the TIF file format. The files are uncompressed, and very space consuming.
7.1.6 Insert Crosshair overlay
To insert a cross hair on top of the live image (or on a captured still image), click the `Cross Hair'.
© 2022 DeltaPix Support

DeltaPix InSight - Manual and Help

7.1.7 Insert Calibrated Grid overlay
To insert a calibrated grid on top of the live image (or on a captured still image), click the `Insert Calibrated Grid' (Shortcut Ctrl-G)

The grid properties, grid size and line color cal be set in the `Calibrated Grid Settings'.
7.2 Capture Image Using the Snapshot Mode
DeltaPix CCD cameras offer snapshot capability. This means that exposure and gain snapshot settings different from the preview settings can be used. This is very useful e.g. for acquiring fluorescence images with long exposure. It is possible to have a relative quick - but lower quality - preview frame rate by setting the gain high and enabling binning. Activate the snapshot window from the menu Settings.

It is recommended to use the Use Snapshot Gain option.
If the acquired images suffer from pixel hotspots, these can be gracefully handled by the Auto Fix Hotspots option.

Still Image Capture 43

The snapshot function makes it easy to acquire an image as in the preview, but in maximum quality, low gain (for low noise), and no binning (for high resolution). The recommended way to use DeltaPix CCD or EXMOR cameras for fluorescence applications is listed below:
Increase the live preview gain to a high value. If the image is not bright enough yet, select binning x2. In the snapshot settings menu, set the gain to 2.5 3.5
This makes it possible to both have a live image with high frame rate, and to capture snapshots with long exposure time (up to several seconds) and low noise at the same time.
Note: Snapshot mode is only available for CCD and EXMOR cameras. This is the default acquisition mode.
7.3 Save Image

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7.3.1 Image Saving in a Gallery

To save the image, click on the disk button and Save in.

7.3.2 Image Saving by Right-clicking the Image
To save the image, click the Save as:

7.3.3 Image Saving using the Standard Menu
To save the image, click on the Save As button

Still Image Capture 45

7.3.4 Image Saving by Right-clicking a Gallery
To save the image, right-click in the destination gallery. Quality and file type can be specified.
7.3.5 EXIF
Exif is a specification for the image file format used by digital cameras. The specification uses the existing JPEG formats, with the addition of specific metadata tags. Only JPEG files are supported. The title, subject, comments, author, and copyright tags can be defined. Leaving a field empty means that the tag is not used. To embed the tags with JPEG files, check the `Embed into JPEG files' checkbox.
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Example of using the tags.

The saved files are shown in Windows file explorer. Note that also Camera Model, Camera Maker and Exposure time can be shown. You may need to configure the file explorer to see all the tags.

8 Calibration
It is important to calibrate your cameras and optics before using them for measurements. Using the program without correct calibrations will result in incorrect measurements.
The optics editor is an advanced tool to configure the optics of a microscope.

Calibration 47
The optics editor
The optics components of a microscope can be configured by parameters like depth-of-field, fieldcurvature and preferred exposure time. Colors are used to indicate the status of a component.
Green will indicate that everything looks good - for an objective this indicates that the a calibration is done. Yellow indicates medium-good. - for an objective this indicates that a calibration was not done, but interpolation from another calibration is used. Red indicated not-good - for an objective this indicates that no calibration is done. Grey indicates unknown or unused state. In the above screen shot the grey "Modus 6ZS-3D" and
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"AB6000" are associated with another camera than the currently used Invenio 6EIII (#26659). Switching to the camera used for these microscopes will show another view:

Calibration 49
8.1 Defining the optical device and magnifications
Select and connect your camera and your objective lens to calibrate. The recommended calibration procedure is described in the following: From System Maintenance in the DeltaPix logo menu, select Edit Optics Configuration:
8.1.1 Make the calibration private or shared
One of the first things to consider is whether the calibration is private to each user, or shared by more users. A user is defined by the Windows login. If the `Shared' is selected, then other users doesn't need to go through the calibration process, but can use existing shared calibrations. Select Private or Shared, by clicking one of the buttons
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Optionally a configuration can be copied from private to shared, or from shared to private. Please note that these copy operations are non-reversible take care.

8.1.2 Add an optical device
Right-click on Optics Configuration to name this system, type the name of the system, then select Add:

8.1.3 Add an optical magnification
Right-click on the new system name to set magnification of the objective lens in use, then select Add, and type the nummerical magnification.

Calibration 51

8.1.4 Add a preferred exposure time for an objective
For each objective the preferred exposure time can be defined. This definition is usefull in cases where the auto exousure leads to undesired image intensity.
8.1.4.1 Relative exposure The relative exposure mode can be defined in two ways: 1. Using only the "%" character Instead of entering a fixed exposure time you can enter a `%' digit. This will make the exposure for this objective relative to the magnification.
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As an example, if the exposure time for a 10x magnification is set to 25ms. If you then change to a 20x objective with the exposure set to `%' then the exposure time will be 50ms.

In this mode the exposure time is simply inverse proportional to the magnification.

Example of setting both fixed and relative exposure time.
2. Using the "%" character and a value. Often the optimal exposure time is not perfectly inverse proportional to the magnification. By entering a value along with the "%" character a more coarse exposure time can be set. As an example, if the exposure time for a 10x magnification with the exposure set to `18%' is set to 25ms. If you then change to a 20x objective with the exposure set to `40%' then the exposure time will be (40 / 18) * 25ms = 55.5ms
Example of using fixed relative exposure.

8.1.5 Add a DOF for an objective
For each objective the Depth of Field can be defined. This definition can be automatically used in `Extended Focus' and `Auto Focus'.

Calibration 53

8.1.6

Adding an information file
Some motorized zoom objectives are partially calibrated by DeltaPix prior delivery. The calibration and zoom motor information is delivered as an objectivename.mzm.xml file. This file must be associated with the objective in the optics editor.

In the open file dialog, select the (as example) Navitar_6x.mzm.xml This associates the motor-zoom-model file to the objective calibration.
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Example optics configuration - the x1 is a motorized zoom objective with an associated description file. The file holds information on:
Zoom range and zoom profile. Motor run and hold current. Limit switch polarity. Predefined click stops. Motor speed [steps/sec]
8.1.7 Defining camera- integrated focus controller Z-calibration
Cameras with integrated motor back-focus can be selected as focus controllers in the same
way as other focus controllers.

Calibration 55
The integrated focus controller is simple to configure, just needs to be selected,
When selected, the focus control will show the actual position, and the arrow buttons will move the focus. The `*' means that the focus is uncalibrated and that the readout value is the raw back-focus distance.
The focus value label can be mouse clicked to obtain special functionality: Double click to set position to zero/reference position. Ctrl-click to go to the neutral back focus position. This is the position that is recommended for XY calibration. Note that depending on the focus calibration, this may
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take a value different from 0.

8.1.7.1 Calibrating the focus controller and definition in the optics definition editor
To make a focus calibration, it is recommended to use the DeltaPix focus calibration unit. It consists of calibration slider mounted precisely on a ramp.

DeltaPix focus calibration unit.
Depending on your optical magnification, select an appropriate scale on the slider - the scale bar should cover as much of the field-of-view as possible. First focus on one end of a slider. In the optics configuration editor, click "Focus Position 1".
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Calibration 57
In the optics configuration editor, click "Focus Position 1".
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Note: After reading the position, the back focus stepper motor position is displayed when hovering the button. So, position 1 is 374 steps in this example.

Then, if possible, focus on the other end of the slider. In the optics configuration editor.

Click "Focus Position 2".
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Calibration 59
Note: After reading the position, the back focus stepper motor position is displayed when hovering the button. So, position 2 is 509 steps in this example.
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Finally enter the real focus distance between Position 1 and Position 2. Due to the geometry (30-60-90 angles) of the calibration device the actual height is ½ of the calibration slider read. So, in this example it is 500um.

Calibration 61

The focus information will show with the objective. The readout shows the focus change per back-focus step. In this example 500/(509 - 374) [um/step] = 3.704 [um/step]

8.1.8

Defining field curvature compensation
Field Curvature, also known as "curvature of field" or "Petzval field curvature", is a common optical problem that causes a flat object to appear sharp only in a certain part(s) of the frame, instead of being uniformly sharp across the frame. This happens due to the curved nature of optical elements, which project the image in a curved manner, rather than flat. And since all digital camera sensors are flat, they cannot capture the entire image in perfect focus.

This especially becomes an issue when capturing topographic images, which may tend to have an unwanted curvature. The compensation which is approximated by a parabola id the difference is focus from center of image towards image corners.

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Example of adding a compensation value of 10um to the 0.5x objective.

Example of a motorized Navitar 6x motorized objective with virtual-fixed objectives at the objectives click-stops. Using the click-stop position will activate the designated compensation values. The field curvature compensation will automatically appear in the 3D topography. I a compensation value is not available, the a slider will appear, for manual compensation.
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Calibration 63
References: https://photographylife.com/what-is-field-curvature https://www.coursera.org/lecture/design-high-performance-optical-systems/what-is-petzvalradius-Yy2ya
8.2 Calibrate One Objective
Select and connect your camera and your objective lens to calibrate. The recommended calibration procedure is described in the following:
8.2.1 Calibration slider
Place a calibration slide in your system, so the markings on the ruler are shown on screen:
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8.2.2 Select the device and magnification to be calibrated

In this example we are goiung to calibrate the `MyOpticalDevice' with the x20 objective.

8.2.3 Draw calibration ruler
Click on Calibrate, and Draw Calibration Ruler. Then, on the image, draw a ruler, covering the calibration marks.
Note that calibration can be done both on the live preview and on a still image.
8.2.4 Calibrate to real world metrics

Click on Calibrate, then insert the true length of the calibration line and choose the right unit
Then select Calibrate this optics objective lens to finish this calibration process:

Calibration 65

The `Motorized objective' should only be checked when calibrating a motorized zoom objective.
8.3 Calibrate a Camera and More Objectives
If you have multiple objective lenses connected on the microscope, then it is possible to use InSight to interpolate or extrapolate the rest and only manually calibrate one objective lens. Please note, that the software only uses one set of true values to calculate the rest by interpolating. This may cause imprecise measurements if the magnification of the objective lenses are not very close to the nominal magnifications. For precise measurements we recommend calibrating every device individually. The best result is achieved if calibration is done using a still image captured at maximum resolution.
8.4 Calibrating a motorized zoom objective
8.4.1 Defining the Objective in the Optics Editor
Initially the motorized zoom objective must be defined in the Optics Editor. The objective should always be defined as a x1 objective.

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8.4.2 Associating the Description file
The motorized objective comes along with a factory calibration file describing it's characteristics.
The file is needed to ensure precise calibration.
This file is an XML file holding the Motor Zoom Model (mzm). This file must be loaded into the optics editor.

In the open file dialog, select the (as example) NEW MS-12HZ-L125-P2.mzm.xml This associates the motor-zoom-model file to the objective calibration.
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Calibration 67

8.4.3

Defining the Depth of Field
The default Depth of Field for the motorized objective is defined in the description file - and normally this is good. Optionally the default Depth of Field can be overridden by defining a new value in the Optics Editor. The entered Depth of Field must be the value at HIGHEST magnification.

Example of an overridden Depth of Field - in this case 25um.

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8.4.4 Calibrating the Objective
Calbrate the objective in its HIGHEST zoom position.

To calibrate, draw a calibration ruler.

The `Motorized Objective' checkbox must be checked.

Enter the correct `Ruler Length'.

Click the `Calibrate this optics objective'.

NOTE: Work careful and precise all precision matters!

Calibration 69

8.4.5

Motor Zoom - Virtual fixed objective positions
The precision of a motorized zoom calibration can be increased at selected positions, these positions can be calibrated as virtual fixed objectives.

The Optics Editor In the optics editor simply add these positions, and calibrate as fixed objectives.

A motorized zoom objective with two fixed calibration positions.x0.5 and x3
For this to work, the selected optics device must be the motorized objective, identified by the x1
Selection of the motorized objective
NOTE: The virtual fixed objectives MUST be calibrated, interpolation will not work!. NOTE: Any position, EXCEPT the x1 can be defined as virtual objective. The x1 is used for the motorized objective definition. NOTE: The precision still depends on the position detection of the limit switches on the motorized objective.

Selection of a magnification with an associated Virtual fixed objective position is indicated with an orange boundary.

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8.5 Calibration overview
The Optics Editor provides an overview of the calibration status.
The color of the objective icon indicates the status of the objective:
Green the objective is calibrated.
Yellow the objective has been calibrated by interpolation from another objective.
Red the objective is not calibrated.

Calibration 71

-
8.6 Calibration Backup and Restore
Backing up the calibration files is a good idea. Backup and restore is also an easy way to move the calibration files from one computer to another.

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8.6.1

Backing up the Calibration files.
To make a backup of your calibration files, follow this procedure. Open the Optics Configuration Editor. Click the `Backup configuration'.

Browse for a directory to store the calibration files. The files can be stored anywhere, e.g. on a portable USB memory. In this example the files will be stored on the D: drive. It is convenient to create a new
directory for each backup click
the `Make New Folder' button.

Name the new directory.

Calibration 73

Mark the directory, click OK the calibration files will be stores almost instantly... All done....

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8.6.2 Restoring the Calibration files.

Restoring the Calibration files.
To restore the calibration files (maybe on another computer), follow this procedure carefully.
Open the Optics Configuration Editor. Click the `Restore configuration'.

Browse for the directory holding the calibration files (a directory made during a calibration backup). In this example the files are present on the D: drive in a directory named `My Calibration Files' Select the directory, and click OK. All done....
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Calibration 75
8.7 Scale Bar
A Scale Bar can be inserted in the lower right corner if an image. This can be done automatically immediately after image capture as described in section `Capture Still Image', or manually afterwards.
Click the Scale Bar button to see the options.:
The Insert Scale Bar will manually insert a Scale Bar. Check the Fixed Length Scale Bar to insert a scale bar of fixed length, as specified (in this example 500 µm). Un-checking the Fixed Length Scale Bar will insert a variable length Scale Bar length is automatically scaled to fit the image. The colour of the bar and font can be set. The font type and size can be set. A semi transparent text background and bold style can be selected to make the text more readable.
8.8 Walton and Beckett graticule
The Walton and Beckett graticule is used for counting fibrous dust (e.g. asbestos or glass fibers) and is particularly useful where the majority of fibers to be counted are shorter than 5 micron. The circle on the Walton & Beckett graticule represent 100 microns at the stage. The circle is divided into four by two diametrical lines scaled in units of 5 and 3 microns respectively. 3 and 5 microns are the critical measurements of fiber lengths used in fiber counting.
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Click the 'Walton + Beckett' to insert. This can be done on live or still images. The graticule will use the system calibration, an re-size accordingly.

Example of a 'Walton + Beckett'.
8.9 Calibrating a motorized Zeiss MTB zoom objective
The DeltaPix InSight supports important functionality from the Zeiss MTB microscope manager. This section describes the calibration of e.g. a Discovery SteReo V12/V16/V20
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Calibration 77
Controlling and read the Focus allowing Extended Focus and Autofocus. Controlling and read of the Zoom allowing very easy calibration. Detection of objective change, the software can automatically switch to another calibration. 8.9.1 Defining the microscope Open the `Optics Configuration Editor' Right click the root `Optics Configuration' Add the microscope in this example a `Zeiss SteREO v12'. Click `Add'
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8.9.2 Defining the microscope magnification

Right click the freshly added microscope.
Enter the actual magnification read from the `Zoom Automation' panel (or from the Zeiss SyCoP).
In this example the magnification is 10.4, a more precise magnification can be read by hovering the magnification, see just below:

Enter the magnification. Click `Add'
If using an encoded nosepiece with more objectives, then the objectives MUST be defined in order first position 1, then position 2 etc.
The objective will appear in red, indicating that is has not yet been calibrated. Click `Save Configuration'. Close the `Optics Configuration Editor'

8.9.3 Calibrating the motorized objective Calibrating the motorized objective To calibrate, draw a calibration ruler.
The `Motorized Objective' checkbox must be checked.
Enter the correct `Ruler Length'.
Click the `Calibrate this optics objective'.

Calibration 79

In the Open the `Optics Configuration Editor' the objective will now appears as a `star', showing that this is a motorized objective with read back of the zoom.

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If using an encoded nosepiece with more objectives, then the objectives MUST be defined in order first position 1, then position 2 etc.

8.9.4 Controlling the microscope

The microscope can now be controlled using the buttons in the `System Automation' panel. The focus can also be controlled by the `PgU' and `PgDn' keyboard buttons.
8.10 Sharing Calibrations
Calibrations are stored on a per-user basis. In some cases, it is advantageous to copy calibrations from one user to another. This section describes how to do this.
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Calibration 81 The names of the directories may vary depending on your regional language. The actual location of the Calibration directory may be different depending on your operating system.
8.10.1 For the user with a complete calibration
When calibration has been done for one user, the calibration files must be copied to the other user. Find the directory holding the calibration data. Click the `Open Application Data Directory' button.
A dialog will open; showing the directories belonging to the actual user, in this (Windows 7) example the user is named `x480'.
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Copy the Calibration directory to a convenient place, like a USB memory stick. Close the DeltaPix InSight
8.10.2 Copy the calibration to another user
Start theDeltaPix InSight from another user account, in this example named `User1'. Open the directory holding the calibration data. Click the `Open Application Data Directory' button.

Calibration 83 Copy the Calibration directory (from e.g. the USB memory stick) to the directory, overwriting the existing Calibration directory. You may need to restart DeltaPix InSight to make the calibration take effect.
9 The Home Tab
The Home tab contains the selection of font tools and tabs arrangement tools.
9.1 The Editing Tools
The editing tools can be used to highlight a selected area. The selected area can be copied, pasted. And dragged A selected rectangular area can also be used for cropping.
9.2 The Formatting Tools
These tools set the colour, font line widths, and more for the annotations and measurements.
9.3 Reporting
A distributable report a report that can be read without using the InSight software can be generated. An annotated image can be made into a report in several ways: Realize: The annotation can be `burned' into the bitmap. Excel report: The image is inserted into an Excel spreadsheet, measurements are also inserted. PDF report: The Excel report can be converted to a PDF document.
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CSV report. A comma separated file containing measurement results.

Note: There is no need for installing Microsoft Excel or a PDF printer; all conversions are done within the InSight software.

9.3.1 Realize
Use the Realize to burn the annotations into the image. This allows viewing of annotated images in most commercial image viewers.

9.3.2 Excel Report
A report can be generated in Excel format. This allows for further processing in the spreadsheet. The reporting uses an Excel template, defining the layout of the report. Image, measurement results and other information is placed in the spreadsheet by identifying tags.
A template must be defined, click the Template button in order to navigate to a template.
After a template has been defined, simply click the Excel report button.
Valid tags are: %IMGS% defines the upper-left corner of image. %IMGE% defines the lower-right corner of image. %MEAS% defines column for measurement results. %UNIT% defines column for measurement units. %DATE% defines date. %TIME% defines time. %CAMERAMODEL% defines camera model. %PICTUREDPI% defines the image DPI. %COL% makes a color marker in the color of the annotations text color.

9.3.2.1 Sample Excel Report template Example of report template, showing usage of most reporting tags.

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9.3.2.2 Sample Excel Report output The resulting report contains image and measurement results.

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Note that the colors of the annotations can be exported to the report, and make understanding easier.
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9.3.3 PDF Report

The Home Tab 89

To make distribution very easy and flexible, a report can be published as PDF.
The PDF report generates a report, using the Excel template, and converts it to PDF.

9.3.4 Comma Seperated Value Report
Alternatively measurement data can be appended to a flat comma separated value (CSV) file. Such a data file can be imported in various programs for further data analysis.
The dropdown defines the CSV:
The name of the report. Data can be appended data from several images and measurements in the same file. The separator can be defined. A timestamp can be written with each measurement result. The file can be viewed.
9.3.5 Exporting CSV data to Microsoft Excel
To export measurement data, first setup the data format.
Set the name of the file to export into click the floppy-disc button. If many measurements are to be exported to the same file, check the `Append to file' check-box. Select the data separator, the `;' (Semicolon) is recommended. A time stamp can be included If system automation is used, the XY position can be added.

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Now measurements can be defined on a still image or even at the live image. Now each time you click the `CSV' button (Shortcut F12), the measurement data is exported to the data file.
Hint: Data can be written using the F12 shortcut. Hint: To have easy and convenient access to the CSV buttons, the can be added to the QAT band.
9.3.5.1 The Comma Separated Value file. This is an example of the data in the CSV file. It contains three measurement sessions. Each session contains time stamp, XYZ position [um] five measurement results (tow angles, and three distances) 2019:05:28 07:45:28;888.984375;um;-798.203125;um;3531.5625;um;16.55;º;34.17;º;158.64;mm;221.74;mm;189.47;mm 2019:05:28 07:45:47;888.984375;um;-798.203125;um;3531.5625;um;24.46;º;27.86;º;119.28;mm;185.91;mm;162.65;mm 2019:05:28 07:46:14;930.3125;um;-798.203125;um;3506.484375;um;31.66;º;21.14;º;143.55;mm;151.49;mm;140.42;mm Note: Angular measurements will always as first measurements, the rest in order of creation.
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The Home Tab 91 9.3.5.2 Import to Microsoft Excel 2007
There are several ways to import the data into Excel. One way to import data into Microsoft Excel: click on Data, then click the From Text button in the Get External Data menu. This will bring up the Text Import Wizard. In the first step, make sure you have Delimited set as the first option. You can also select which row you would like to start from. In this example, we're doing the whole thing starting with the first row.
The example data are separated using the ';'. This will then show your data in columns at the bottom.
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The third step simply lets you choose the format of the data, such as numbers, text, or dates. After you've done that, just click Finish and the data will be imported into your spreadsheet.

The Home Tab 93
9.4 The Window tools
The Close button can be used to close all still images. The dropdown allows closing images only of a specific type, e.g. Extended Focus images. The Open behind button makes sure that new images are opened behind the live preview image. This is a useful feature for e.g. manual and automatic Extended Focus.
10 The View tab
This tab holds functions for image viewing, e.g. fit, stretch, zoom etc. Properties and Flip rotate function are adjustment tools for captured images. TDI / MDI switches between two types of arrangements for image viewing.
10.1 Image Tile
In the View tab, there is an option for arranging images in an easy way. Toggle tabbed - MDI View (Ctrl-Shift-T) Tile Vertical (when in MDI view) (Ctrl-ShiftV) Tile Horizontal (when in MDI view) (Ctrl-
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Shift-H) Arrange (when in MDI view) (Ctrl-Shift-A)

11 The Measurement and Annotation tab
Use the Formatting tools from Home to change the font/line colour or size of the annotations or measurements.
11.1 Annotation
Annotations are notations added to the live preview or to a captured image. Choose the type of annotation in the Basic menu, then draw on the image: Use the formatting tools from Home to change font/line.

11.2 Measurement

The Measurement and Annotation tab 95

This is a tool for measuring distances, area, circle properties and angles.
To measure choose the tool, then draw on the image:
The calculated results will automatically show next to the drawing. Use the formatting tools from Home to make font/line adjustments as desired. To repeat a measurement type without mouse clicking on the measurement again, press the space bar.

11.2.1 Polygon and Freehand result options
The Polygon and Freehand tools can measure area and/or perimeter. The selection is done in the drop-down - as shown below.

11.2.2 Circle measurement result options
The circle measurement can display either radius, diameter or perimeter as measurement result. Selection is done via the drop down menu of the Circle button.
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11.2.3 Flexible position for measurement result
For most measurements, the measurement result can be moved around, independent of the position of the measurement tool.
Drag the measurement data using the mouse.
11.3 Save the Annotations and Measurements
There are several ways of saving the annotations and measurements.
11.3.1 Save and Open the Annotations only
Annotations from an image can be saved without saving the image. This is useful if some complicated annotation is used on several images. The annotation can be opened and applied to another image.

The Measurement and Annotation tab 97
11.3.2 Save the Annotations and Measurements in Seperate Files
This is done in the same way as saving an image; see section `Save Image'. The annotations and measurements belonging to the image will appear automatically when using InSight to open and view this image, and editing is possible. Other image viewers will only show the original image without the annotations and measurements.
One of the ways to save the file is to click on the disk button located in the galleries. Hereby, the image and the measurements and annotations will be saved in separate files.
11.3.3 Save the Annotations and Measurements in the Same File
Another way is to save the image and the annotations and measurements in one file:
The program will burn the annotations and measurements on the original image, so a new image is created. In this way the annotations and measurements will no longer be editable.
12 The Still Image Tab

In the Still Image menu there are several adjustment tools available for changing the specifications of a captured image. There are functions for noise removal, intensity remapping and much more.

12.1

Undo
Operations done in the `Still Image' panel can be undone. There are two possibilities, undo the most recent operations or undo all operations and revert to the original image.

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The shortcut for undo is `Ctrl-Shift-Z'.

12.2

Remap Intensity
In some cases it is desirable to be able to remap the pixel intensities. This can remove some kind of noise or increase the contrast. The `Remap Intensity' button has two options, `Remap Intensity' or `Remap Intensity Settings'. The `Remap Intensity' used the settings from the `Remap Intensity Settings'.

The `Remap Intensity Settings' opens a panel with several adjustments.

The star button resets the settings to default, a 1:1 mapping. The 100% button shows the `After' image in its real size; use the mouse for manoeuvring around.

The Still Image Tab 99

12.3

Fluorescence
Images captured can be fake colored and combined. This is useful when dealing with fluorescence images captured with a monochrome camera.

An image can be forced to another colour using the dropdown menu as shown here:

The images can then be combined; a good result is achieved using the Maximum option.
This is an example of combining two fluorescence images; one fake coloured to green, the other fake coloured to red.

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12.4

Deconvolution Lucy-Richardson algorithm
Functions perform image deconvolution. They can be used for restoring the degraded image, in particular image that was obtained by applying the convolution operation with known kernel. (Iterative method) [Ric72]. Border pixels of a source image are restored before deconvolution.

For further reference to the Lucy- Richardson method: W.Richardson. Bayesian-Based Iterative Method of Image Reconstruction. Journal of the Optical Society of America, vol.62, No.1, January 1972.

Note that to deconvolve an image, the image should NOT be saturated.

Example of blurred image

The Still Image Tab 101

After applying the deconvolution, some details now appear clearer.
13 The Image Extender Tab
The Extended Focus and Extended Exposure functions can create an image, where multiple depths and multiple exposures are all in focus at once by putting the different layers together to one image. Images can also be combined using Extended Field (a.k.a. stitching). The tab also has the option for Extended Time Recording (a.k.a. Time lapse recording).

13.1

Manual Extended Focus
InSight has a state of the art Extended Focus function. Both manual and automatic Extended Focus is possible. The automatic function uses the motor automation.

Capture several images from top to bottom of the different focus levels, (increase or decrease focus with as equal intervals as possible):
Then select Execute. The progress bar will show the ongoing work.

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Live preview

Extended Focus image

Note: Using many large size images may cause the process to run out of memory.

13.2

Extended Exposure
Extended Exposure combines multiple images, each captured with different exposure time and thus having only a part of the image correctly exposed.

Select in Settings the amount of images desired (max. five), then select the different exposure percentages:
Now, select Execute and the Extended Exposure image will appear after a short while.

Live preview:

Extended Exposure image:

The Image Extender Tab 103
13.2.1 Contrast post processing
The merging of several images acquired at different exposure time sometimes degrades the contrast. In such cases it may be useful to do an automatic contrast enhancement. Check the 'Contrast post-processing' check box.

The slider sets the degree of contrast enhancement - a value around 35% often leads to a very good result.

13.3

Manual Extended Field
Images captured using e.g. a manual XY stage can be combined using the Extended Field tool. With the intuitive user interface manual, stitching of single captured images can be performed fast and precise. Simply define the number of rows and columns and drag the images from the directory to the graphical guide and press the execute button to perform the stitching.

It is very important that the images are carefully aligned horizontally and vertically if not the resulting image will be poor or wrong.

104 DeltaPix InSight - Manual and Help To combine the images select the number of rows and columns. The overlap between the images must be set to the overlap of the acquired images (at least 10% is recommended). Deviation is the `uncertainty' of the overlap.
Next, click the Organize button and drag images from a gallery to the Extended Field editor.
Images can be re-arranged within the editor by dragging. This example shows how to swap the position of two images.
When the images have been arranged, click the Execute button. Note that combining the images may take a while.
© 2022 DeltaPix Support

The Image Extender Tab 105
Using many large size images may cause the process to run out of memory.
14 The Counting Tab [from v6.1.18]
The counting tab holds all controls to set up a counting session.
The counting utilizes up to four phases. A phase is defined by a set of features, like color range and geometrical features. The geometrical features can be:
Individual for each phase. This makes counting of objects with different geometrical features possible. Shared. This means that all phases use the same geometrical features. Used when counting objects only segmented by color. The selection of individual or shared features is done in the settings drop down.button, see "Use same features"
© 2022 DeltaPix Support

106 DeltaPix InSight - Manual and Help

14.1

Workspace
A workspace can be saved or opened. This is useful when counting different samples or using different setups.

Counting workspace's are saved with file extension cnt.xml, so a workspace could have name like myWorkspace.cnt.xml.
Save workspace

Load workspace

The Counting Tab [from v6.1.18] 107
14.2 Phase Segmentation
The segmentation is based on threshold RGB ranges.
The phase is defined by an RBG range, which can easily be set by three range sliders. In this example phase #3 has RGB range [0-64][95-220][9-73].
14.2.1 Pick object color
The threshold range can be set individually for each color channel. Using the color picker, makes it easier to find an appropriate threshold range, some fine tuning may be needed.
When picking a region, a margin can automatically be added. In this example the lower threshold range will be 16 lower, and 16 higher than the picked value.
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108 DeltaPix InSight - Manual and Help
14.2.2 Phase Definition Overlap
If phases are defined so that the definition ranges overlaps, an exclamation sign is shown next to the sliders.
A phase overlap means that a region belongs to more than one phase.
14.3 Morphology preprocessing
As a pre-processing option it is possible to do binary morphology i.e. after doing the threshold.

Morphology is useful for removing small artifacts, or disconnecting regions. Note: Using same shrink and expand level, will approximately preserve the object area.

14.4

Area Feature
The counting can use the area as discriminator. The enable this, check the checkbox, and set the sliders to the area range to include in the counting.

Example: Only objects with an area in the range 12um² 36.04mm² will be included in the counting.
14.5 M1 Feature
The counting can use the M1-parameter as discriminator. The M1 is a size independent moment-based feature describing the compactness og the object.
A perfect round compact object will measure 15 A "long stripe" will measure 70+
© 2022 DeltaPix Support

The Counting Tab [from v6.1.18] 109 The enable this, check the check box, and set the sliders to the area range to include in the counting.
Example: Only objects with an M1 in the range 15 27 will be included in the counting. This will exclude "long" objects. For more information on the rotation and size invariant M1 feature, please see: Using Image Moments for Tracking Rotating Objects, ARAS 05 Conference, 19-21 December 2005, CICC, Cairo, Egypt, Payman Haqiqat Islamic Azad University (IAU) Dubai Campus.
14.6 Ferret Feature
The method corresponds to the measurement by a slide gauge. It is defined as the distance between two parallel tangents of the particle. The minimum Feret diameter is often used as the diameter equivalent to a sieve analysis.
Example: Only objects with an Feret min = 31523um and max in the range 134674um will be included in the counting.
These parameters, min and max, can be used to exclude the counted objects via the Ferrets diameter interval.
Please note that the calculation of the min Feret is complicated, and thus a little slow
© 2022 DeltaPix Support

110 DeltaPix InSight - Manual and Help
14.7 Counting
The Count panel holds a Settings, Preview and Count button.
Clicking the Preview button will preview the actual phase so that the phase definition can be finetuned.
14.7.1 Settings
In the settings button drop down, the mark color can be defined. Each phase color, and the colors for ignored objects can be set. Also, the option of ignoring objects touching the image border is set here.
© 2022 DeltaPix Support

The Counting Tab [from v6.1.18] 111
14.7.1.1 Use same features The geometrical features can be: Individual for each phase. This makes counting of objects with different geometrical features possible. Use same features. This means that all phases use the same geometrical features. Used when counting objects only segmented by color.
© 2022 DeltaPix Support

112 DeltaPix InSight - Manual and Help
14.7.2 Counting Options
The Settings drop out button is defining the basic result presentation
14.7.2.1 Create Table Checking this, will make a table pop-up with detailed counting data when the Count button is clicked.
Table with detailed counting information. The color in the leftmost column is the same as used to colorize the individual phases.
© 2022 DeltaPix Support

The Counting Tab [from v6.1.18] 113 14.7.2.2 Insert Count Label
Checking this, will insert a basic label in the lower left corner of the counting image with the number of objects and the area percentage for each active phase.
14.7.2.3 Create New Image Will create a new counting image from the still or live preview image. Otherwise the still image will be overwritten.
14.7.2.4 Object index label Checking this, will insert a counting number on each object. Please note that this feature is very slow, and when counting many objects it will add significantly to the processing time.
14.8 Reporting
A report can be generated in Excel format. This allows for further processing in the spreadsheet. Excel reports can be generated even without a Microsoft Excel installation on the computer. The reporting uses an Excel template, defining the layout of the report. Image, measurement results and other information is placed in the spreadsheet by identifying tags.
14.8.1 Excel
A template must be defined, click the Template button in order to navigate to a template.
© 2022 DeltaPix Support

114 DeltaPix InSight - Manual and Help Checking the "Open Report" will automatically attempt to open the Excel report after generation. After a template has been defined, simply click the "Excel" button to create a report from a counting.
14.8.2 Template tags
Tags can be inserted in the Excel template document to define the layout of the report. %AREA% will insert the area for the object %AREA_UNIT% will insert the area unit for the object %M1% will insert the M1 for the object %FERRET1% will insert the Feret minimum distance for the object %FERRET1_UNIT% will insert the Feret minimum distance for the object %FERRET2% will insert the Feret maximum distance for the object %FERRET2_UNIT% will insert the Feret maximum unit for the object %PHASEPCT% will insert the area percentage for the object %PHASECOL% will colorize the cell with the actual phase color. %PHASEIDX% will insert phase index for the object, i.e. which phase it belongs to. %ANG% will insert minimum inertia angle of the object. For compact objects, this of course is unpredictable. %ANG_UNIT% will insert angle unit of the object. %DIAM% will insert the diameter of circle with same area as object. %DIAM_UNIT% will insert the diameter unit
14.8.3 PDF
To make distribution very easy and flexible, a report can be published as PDF.
The "PDF" generates a report, using the Excel template, and converts it to PDF. Checking the "Open Report" will automatically attempt to open the PDF report after generation.
© 2022 DeltaPix Support

The Counting Tab [from v6.1.18] 115
Example of PDF report
© 2022 DeltaPix Support

116 DeltaPix InSight - Manual and Help
14.8.4 Excel processing example calculating the Phase%
The advantage of exporting to Excel, is that further processing easily can be done.
As example: If the phase number (by tag % PHASEPCT %) is written to A39. This will write the phase numbers to column A, starting from A39. If the phase percentage (by tag %PHASEIDX%) is written to B39. This will write the phase percentage to column B, starting from B39.
Then the total phase percentage for phase "1" can be calculated like "=SUMIF($A39:$A100, "1",$B39:$B100)"

Sample template with data processing.

14.9

Manual Counting
Some objects can be too difficult to count automatically. The manual counting can be used in such cases.

The 'Mark' button will create a new counting point that can be placed onto the image.

The Counting Tab [from v6.1.18] 117
When the first counting point has been placed, the subsequent points can be created by hitting the space keyboard button. The count value is normally updated automatically.
15 The System Automation Tab
In this tab the XY and focus motor can be controlled. This is done by using the arrow buttons.
It is also possible to use the keyboard arrow keys for XY-stage control, and the PgUp and PgDn buttons for controlling the focus. Click on the live preview image to enable this feature.
15.1 Stage Automation
The Stage Automation is used for controlling a motorized XY stage.
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118 DeltaPix InSight - Manual and Help
This can be used for the automated Extended Field tool or for capturing images at different positions at certain intervals.
15.1.1 Selecting and Configuring the Motor Controller
Click the small dialog launcher to configure the motor controller.
15.1.1.1 Prior Controller Prior controllers connect via a virtual COM port. Enter the COM port number and click `Connect'.
© 2022 DeltaPix Support

If connection succeeds, then just above the COM port, the type of the attached stage will be shown. In this example an ES111 stage is attached.

The System Automation Tab 119

If the controller has joystick control, then automatic read back of position can be enabled by checking the `Has joystick control' checkbox.
The tools dropdown shows the temperature and power supply of the controller. The Standby and Running currents can be set.

120 DeltaPix InSight - Manual and Help 15.1.1.2 Zeiss MTB
Zeiss MTB Chose the direction scheme that fits your physical setup.
NOTE: Requires a well configured MTB2011 server. See Installing and connecting to Zeiss MTB 2011 15.1.1.3 Ludl MAC5000/MAC6000 Controller Ludl MAC6000
© 2022 DeltaPix Support

Before connecting the controller, set the communication to the Ludl default settings.
Please note that other settings may be required depending on the controllers configuration, consult the controller documentation for more information on this.

The System Automation Tab 121

Connect to the controller.
When the controller has successfully connected the `Connect' button will stay orange.
A text will show the name and version of the controller.
In addition a popup will show when hovering the connect button.

122 DeltaPix InSight - Manual and Help
Chose the direction scheme that fits your physical setup.
If the controller has joystick control, then automatic read back of position can be enabled by checking the `Has joystick control' check box.

15.1.1.4 Märzhauser TANGO
Before connecting, the appropriate COM port must be selected. Normally the COM port will have friendly name "TANGO"
STAGE XY EXAMPLE CONFIGURATI ON: XY-AXIS 315 STEPS/ MM, 200 STEPS/REV MOTOR.
NOTE: FOR THE LIMIT SWITCH OPTION YOU MAY SELECT NONE, THEN THE DEFAULT OPTION (AS

SEEN IN THE MÄRZHÄUSER SWITCHBOAR D) IS USED. When connection is successfully established, the icon will change, and the serial number of the MÄRZHÄUSER TANGO will be shown in the top of the form.
After this, the DeltaPix InSight will automatically connect to the MÄRZHÄUSER TANGO when the program is started.
THE MOTOR POWER OPTION MUST BE CAREFULLY DEFINED TO MATCH THE MOTORS.
IF USING USB CONNECTION, THE BAUD RATE IS OBSOLETE.

The System Automation Tab 123

124 DeltaPix InSight - Manual and Help 15.1.1.5 DeltaStep4
The controller communicates via a virtual serial port. This is easily identified as a `Silicon Labs CP210x' device.
If this is not already automatically selected, select it and click connect.
When successfully connected, a green check mark is shown.
The tools drop-down shows the power supply of the controller. Both Running and standby current can be set. The polarity of the limit switches can be set. To change the polarity check-box value, the shift key must be pressed while clicking this is to prevent unintended change. Utmost care must be taken selecting the wrong polarity may damage your motor device!
If the controller has joystick control, then automatic read back of position can be enabled by checking the `Has joystick control' check-box.
© 2022 DeltaPix Support

15.1.1.6 Mitutoyo - MF
The controller communicates via a virtual serial port. This name of this may vary - in this example a CH340 USB-RS232 converter is used.
If this is not already automatically selected, select it and click connect.
When successfully connected, a green check mark is shown.

The System Automation Tab 125

PLEASE NOTE: The Mitutoyo controller is read-only, so selecting this will only display the actual position.

Manipulating the position can only be done directly on the microscope.
15.1.1.6.1 Mitutoyo - Insert Scale Bar
Enter topic text here.By selecting the 'Insert Scale Barp' option, XY and focus position information will be added to the Scale Bar.

126 DeltaPix InSight - Manual and Help
This will insert a scale bar into captured still images including the XY and focus position.
15.1.1.6.2 Mitutoyo - EXIF Information
The XY and focus position will also be written into the EXIF header of the image.
15.1.2 Making the motor speed relative to the Magnfication
Most motor controllers can be configured to make their XY speed relative to the magnification. In practice this sets the speed lower if the magnification is set higher. As example a 20x magnification will run at 25% of the speed of a 5x magnification. The speed set in the XY controller panel will be used for the lowest magnification.
© 2022 DeltaPix Support

The System Automation Tab 127
15.1.3 Verifying the X and Y movement scheme
To make the automated scanning functions work, it is important that the movement scheme is correct. This is easily checked. Sample with an object approximately in the middle of the preview image.
The `Arrow Right' button must move the FOV to the right; this should make the object move to the left as indicated in the image.
© 2022 DeltaPix Support

128 DeltaPix InSight - Manual and Help The `Arrow Up button must move the FOV upwards; this should make the object move down as indicated in the image. .
15.2 Automatic Extended Field
For automated Extended Field, move by the arrow buttons to the corners of the area-of-interest.
Select the region-of-interest, and click the pin... The required number of images is automatically calculated note that the camera and objective needs to be calibrated. The `Extended Field' button Hint will show the number of images to be acquired.
Click the Extended field button to start.
© 2022 DeltaPix Support

The System Automation Tab 129
Using very many large size images may cause the process to run out of memory.
15.2.1 Automatic Extended Field with Extended Focus and Extended Exposure
The dropdown has checkboxes for including Extended Exposure and Extended Focus.

15.3

Automatic XYZ Acquisition
XYZ Acquisition can acquire images at predefined positions at regular intervals. This is useful for monitoring long term activity at different locations, e.g. in a Petri dish.

The XYZ positions for each image are stored in a table. The table can be saves and loaded.
To store the XYZ entries in the table click the pin button. Then by clicking the arrow button the XY-stage and Z-motor will move to the marked position.

130 DeltaPix InSight - Manual and Help
Up to 500 positions can be stored.
15.3.1 Acquisition Options.
The Acquisition options define the acquisition period.
If e.g. the acquisition rate is set to 10 per minute and the number of acquisitions is set to 20, the total acquisition duration will then be 20 [acquisitions] / 10 [acquisitions/minute] = 2 minutes. In a period of 2 minutes 10 images will be acquired at each position. When the acquisition has completed, the images can fairly easy be combined to a lapse time video. Use e.g. the ImageJ program from www.nih.com At each position the images can be acquired using Extended Exposure and Extended Focus tools.
© 2022 DeltaPix Support

15.3.2 Define the target image directory.
In the `File options' click the floppy-button to define a target image directory to store the images.
Images can be saved in a variety of formats, 2D or 3D.
For the 2D.jpg, also select the image quality, it is recommended to click the rightmost star to have the best image quality.

The System Automation Tab 131

15.3.3 The position table.
Positions can be defined by moving the XY-stage to different positions, and then click the `pinbutton' for each position.
15.3.4 Defining Image Position macros in the `Comment' field.
If images are to be acquired at fixed grid positions, a macro can be written in the Comment field. Move the stage to the reference position, and define the macro, then click the `pin-button' associated with the reference point. All the positions will the automatically be calculated.

Command refxy
refxys
n: sets nx and ny

Usage
Make this point a reference point position is ZigZag patten organized.
Make this point a reference point position is Snake pattern organized. This is travel optimized to nearest next position, so focus should have minimum change.

nx (default=1) ny (default=1) d: sets dx and dy
dx (default=1)

Sets number of columns (x direction). Example "nx=4" Sets number of rows (y-direction). Example "ny=4"
Distance between column starting position in microns (x direction).

132 DeltaPix InSight - Manual and Help

dy (default=1) ff+ dt

Example "dx=100"
Distance between row starting position in microns (y direction). Example "dy=100"
Do not use focus position. The "f-" option is useful for surface focus tracking via the auto focus function. NOTE: Works for all positions after the position where it is defined, or until "f +".
Use focus position. Used to switch off the "f-" option.
Sets a delay in ms after stages have settled, and before image capture. Example "dt=1000". NOTE: Works for all positions after the position where it is defined. Disable by "dt=0"

In the comment field type the script. Ex1: "refxy nx=10 dx=2000" sets 10x1 positions in a 2000x0 um grid Ex2: "refxy nx=10 ny=3 dx=2000 dy=5000" sets 10x3 positions in a 2000x5000 um grid Ex3: "refxy n=3 d=5000" sets 3x3 positions in a 5000x5000 um grid Ex4: "refxy n=3 dx=1000 dy=2000" sets 3x3 positions in a 1000x2000 um grid
Ex5: "f- dt=1200" For this and the following positions do not use the focus position (maybe to rely solely on the autofocus tracking), and after XYFZ stage settles, wait ~1200ms before image acquisition.
Click the 'pin' button, all the positions will then be re-calculated with reference to the actual ref (x,y) position
Example: refxy nx=5 ny=3 dx=2000 dy=5000

The System Automation Tab 133

A simple example of 15 images to be acquired at a distance of 2000um in the x-direction and 2000um in the y-direction. The {A} and the bluish color indicates that this is a macro calculated point.

15.3.5 Defining Point Transformation macros in the `Comment' field.

Command Usage

P=0

R0 - Coordinate origin

P=10

R10 - is the same point as R0, but used for shift compensation and accumulated stage error.

R=1

R1 Reference point on the left side corresponding to R2 on the right

R=2

R2 - Reference point on the right side corresponding to R1 on the left

R=3

R3 - Reference point on the left side corresponding to R4 on the right

R=4

R4 - Reference point on the left side corresponding to R3 on the left

G=5

G5 A data point on the left side. A corresponding G6 point will be calculated. Several G5 points can be defined.

134 DeltaPix InSight - Manual and Help

G=6

G6 - A data point on the right side. A corresponding G5 point will be calculated. Several G6 points can be defined.

The drawing below explains the principles:

This table show the principle of the coordinate transformation macros.

Drawing courtesy of Maria Giuditta Fellin, ETH Zurich

For more information on this topic, please contact support@deltapix.dk
15.3.6 Table Macro programming
Table Macro programming
© 2022 DeltaPix Support

The System Automation Tab 135 The position table can easily be modified manually. The smarter way is to do it automated by writing a specialized macro. Advanced operations and transformations can be performed. The macro language is C# - and writing a macro requires some programming skills. Open the macro editor. The button name `MyCaption' can change programmatically in the macro to a more intuitive name.
Please contact support@deltapix.dk for help with your specific macro programming if necessary. Click the `Edit' button to enter edit mode. The text window will turn yellowish.
© 2022 DeltaPix Support

136 DeltaPix InSight - Manual and Help
© 2022 DeltaPix Support

The System Automation Tab 137 As example we change the Caption function this will change the macro button name. Click `Build' and hopefully the build is successfully.
The button name will reflect the macro change.
© 2022 DeltaPix Support

138 DeltaPix InSight - Manual and Help
Macro error In case of a syntax error, the line number and some text is displayed in the upoper right window. In this example there is a trailing `m' character problem in line 10.
© 2022 DeltaPix Support

The System Automation Tab 139
15.3.6.1 Table Macro Programming - Example code
public Exception ProcessMacroTable(int nRows, ref bool[] chkBox, ref float[] x, ref float[] y, ref float[] focus, ref float[] zoom, ref string[] Macro, ref System.Drawing.Color[,] colCell)
{ try { float refX = 0; float refY = 0; float refFocus = 0; float refZoom = 0; for (int r = 0; r < nRows; r++) { if (null != Macro[r]) { // Parse and color code Macro-field string cmt = Macro[r]; System.Drawing.Color colMacro = System.Drawing.Color.White; if (cmt.Contains("Hello") == true) { colMacro = System.Drawing.Color.Yellow; chkBox[r] = true; } if (cmt.Contains("Goodbye") == true) { colMacro = System.Drawing.Color.Pink; chkBox[r] = false; } // Specify a test macro M0 - store data if (cmt.Contains("M0") == true) { Macro[r] = "M0 REF"; refX = x[r]; refX = y[r]; refFocus = focus[r]; refZoom = zoom[r]; }
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140 DeltaPix InSight - Manual and Help
// Specify a test macro M1 if (cmt.Contains("M1") == true) {
colMacro = System.Drawing.Color.Lime; Macro[r] = "M1 ADD"; x[r] = x[r - 1] + refX; y[r] = y[r - 1] + refY; focus[r] = focus[r - 1] + refFocus; } // Specify a test macro M2 if (cmt.Contains("M2") == true) { colMacro = System.Drawing.Color.Cyan; Macro[r] = "M2 MUL"; x[r] = 2 * x[r - 1]; y[r] = 3 * y[r - 1]; focus[r] = 4 * focus[r - 1]; } colCell[r, 3] = colMacro; // Color code X-fields colCell[r, 0] = (x[r] > 10) ? System.Drawing.Color.SpringGreen : System.Drawing.Color.PaleGreen; // Color code Y-fields colCell[r, 1] = (y[r] > 10) ? System.Drawing.Color.SpringGreen : System.Drawing.Color.PaleGreen; // Color code Z-fields colCell[r, 2] = (focus[r] > 10) ? System.Drawing.Color.SpringGreen : System.Drawing.Color.PaleGreen; } } } catch (Exception ex) { return ex; } return new Exception(null, null); }
15.4 Focus automation
The Focus Automation is used for controlling a motorized Focus stage.
This allows for automated Extended Focus, Automated Topography and Auto Focus.
© 2022 DeltaPix Support

The System Automation Tab 141
15.4.1 Selecting and Configuring the Motor Controller
The Focus automation is used for controlling a motorized Z stage. This can be used for automated Extended Focus. Click the small dialog launcher to configure the motor controller.
15.4.1.1 Prior Controller Prior controllers connect via a virtual COM port. Enter the COM port number and click `Connect'. Note that this controller is not yet connected see the `plug icon'.
If connection succeeds, the plug icon changes status to connected plugs. Check the `Has focus knob' for automatic read back of position. The movement direction determined by the up/down buttons can be reversed by checking the 'Host Direction' check box. The movement direction determined by the focus knob can be reversed by checking the 'Knob Direction' check box.
© 2022 DeltaPix Support

142 DeltaPix InSight - Manual and Help The tools drop down shows the temperature and power supply of the controller. The Standby and Running currents can be set. In case of custom focus motor, the Steps/mm parameter can also be set to ensure correct Z scaling.
See appendix 1 for configuring Prior OptiScanII controller.
15.4.1.2 Zeiss MTB
When connection succeeds, the plug icon changes status to connected plugs. Check the Encoded objectives for automatic read back of selected objective, note that this also impacts the calibration requirements.
Note: Prior controllers can also connect via the Zeiss MTB server. NOTE: Requires a well configured MTB2011 server. See Installing and connecting to Zeiss MTB 2011
© 2022 DeltaPix Support

15.4.1.3 Ludl MAC5000/MAC6000 Controller Ludl MAC6000

The System Automation Tab 143

144 DeltaPix InSight - Manual and Help Connect to the controller. When the controller has successfully connected the `Connect' button will stay orange. A text will show the name and version of the controller. In addition a popup will show when hovering the connect button.
Chose the direction scheme that fits your physical setup. If the controller has a fine focus knob control, then automatic read back of position can be enabled by checking the `Has focus knob' check box.
© 2022 DeltaPix Support

If an absolute encoder is not used, it may be necessary to scale the position read from the controller. This is done via the optional scale factor. NOTE: The controller must be disconnected in order to modify the scale factor. Default value is 1.00
The scale factor can also be entered via the calculator drop down menu.

The System Automation Tab 145

146 DeltaPix InSight - Manual and Help 15.4.1.4 Märzhauser TANGO
Before connecting, the appropriate COM port must be selected. STAGE FOCUS EXAMPLE CONFIGURATION: Z-AXIS 800 STEPS/MM, 200 STEPS/REV MOTOR.
NOTE: FOR THE LIMIT SWITCH OPTION YOU MAY SELECT NONE, THEN THE DEFAULT OPTION (AS SEEN IN THE MÄRZHÄUSER SWITCHBOARD) IS USED.
When connection is successfully established, the icon will change, and the serial number of the MÄRZHÄUSER TANGO will be shown in the top of the form. After this, the DeltaPix InSight will automatically connect to the MÄRZHÄUSER TANGO when the program is started. THE MOTOR POWER OPTION MUST BE CAREFULLY DEFINED TO MATCH THE MOTORS. IF USING USB CONNECTION, THE BAUD RATE IS OBSOLETE.
© 2022 DeltaPix Support

15.4.1.5 DeltaStep4
The controller communicates via a virtual serial port. This is easily identified as a `Silicon Labs CP210x' device.
If this is not already automatically selected, select it and click connect.
When successfully connected, a green check mark is shown.

The System Automation Tab 147

The tools drop-down shows the power supply of the controller. Both Running and standby current can be set.
The polarity of the limit switches can be set. To change the polarity check-box value, the shift key must be pressed while clicking this is to prevent unintended change.
Utmost care must be taken selecting the wrong polarity may damage your motor device!

If the controller has focus knob control, then automatic read back of position can be enabled by checking the `Has focus knob' checkbox.
If the controller has a built-in encoder wheel, check the `Has Encoder Control'. Otherwise leave unchecked.

148 DeltaPix InSight - Manual and Help 15.4.1.6 Mitutoyo - MF
The controller communicates via a virtual serial port. This name of this may vary - in this example a CH340 USB-RS232 converter is used. If this is not already automatically selected, select it and click connect. When successfully connected, a green check mark is shown.
PLEASE NOTE: The Mitutoyo controller is read-only, so selecting this will only display the actual position.
Manipulating the position can only be done directly on the microscope.
15.4.1.6.1 Mitutoyo - Insert Scale Bar
By selecting the 'Insert Scale Barp' option, XY and focus position information will be added to the Scale Bar.
© 2022 DeltaPix Support

The System Automation Tab 149 This will insert a scale bar into captured still images including the XY and focus position.
15.4.1.6.2 Mitutoyo - EXIF Information
The XY and focus position will also be written into the EXIF header of the image.
© 2022 DeltaPix Support

150 DeltaPix InSight - Manual and Help 15.4.1.7 ErgoFocus
NOTE: ErgoFocus will only work on Windows 10/11. Windows 7 or 8 will not install correct drivers!
The ErgoFocus controller communicates via a USB port (virtual serial port). This is automatically identified (from the unique VID/PID).
When successfully connected, a green check mark is shown.
Steps/mm The Steps/mm parameter must be set to correct Z scaling. It is calculated as: motor[steps/rev] / Spindle[mm/rev]. Example: Stepper motor has 200[steps/rev], and spindle pitch is 0.25[mm/rev], this makes the Steps/mm parameter = 200 / 0.25 = 800.
© 2022 DeltaPix Support

The System Automation Tab 151 Ramp Up/Down This sets the start/stop acceleration ramp. The bigger number, the slower acceleration. A value of 0 means 'instant' start/stop. Swap Direction Reverses the motor direction Swap host direction Reverses the sign of the position readout Limit switch Normally Open The polarity of the limit switches can be set. The default is "Normally Open". Standby- and Running current Adjusting the current requires great care and diligence. DeltaPix can provide instructions on how to do this. Please contact support@deltapix.dk.
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15.4.1.7.1 Stand alone mode - parameters
Requires ErgoFocus firmware 1.4 or higher.
For standalone the parameters must be set using DeltaPix InSight. DeltaPix InSight. Connect to the ErgoFocus controller, and set parameters as needed.
DeltaPix ErgoFocus: Press joystick button (~5secs) to see/check the non-volatile parameters
[V] = Checked
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15.4.1.7.2 ErgoFocus firmware versions

The System Automation Tab 153

ErgoFocus firmware is developed by DeltaPix. To make it work with DeltaPix InSight some version matching must be observed.

15.4.1.8 Camera with integrated focus control Some cameras like eg. the H4KWSD08DPX has integrated back focus motor. This can be selected as focus motor controller.
Different modes focus modes can be selected. The manual focus should be selected to use the integrated focus automation for creating extended focus images.

15.4.2 Automatic Extended Focus
Use the arrow buttons to the upper and lower focus positions, and mark each position by pressing the pin buttons. When top and button Z-positions have been defined, click the Extended Focus button to start the automatic Extended Focus.
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154 DeltaPix InSight - Manual and Help The best result is achieved using telecentric objectives. Non-telecentric objectives and stereo microscopes may require an increase of the default parameters in the Extended Focus Settings dropdown.
15.4.2.1 Setting the Number of Z-slices to be acquired. The number of images is defined in the `Image Extender tab. In the Extended Focus Settings dropdown set the number of slices. This is shown here.
Using many large size images may cause the process to run out of memory. 15.4.2.2 Using the DOF from the Optics Editor
If a DOF for the objective has been defined, then the number of required slices can be automatically calculated.
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The System Automation Tab 155
15.4.3 Automatic Extended Focus with Extended Exposure
To combine Extended Focus with Extended Exposure, check the Extended Exposure checkbox. The process may take some time. The progress bar shows the ongoing work.
15.4.4 Auto Focus [from v6.6.7]
Click the `Settings' button to configure the autofocus function.
NOTE: By moving the mouse to the info-balloons, a brief explanation for the parameters is provided
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156 DeltaPix InSight - Manual and Help If the objective used has defined `DOF' defined in the `Optics Editor', then this will be used as the actual DOF. Some motorized zoom objectives automatically updates their DOF. Check this check-box if the DOF is defined with the objective in the optics editor.
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This is the default DOF is used if no specific DOF is defined for the objective
A narrow DOF will make the auto focusing slowly a wider DOF makes it faster.

The System Automation Tab 157

158 DeltaPix InSight - Manual and Help The `Search Range' defines the maximum interval to look for a good focus. Note that the value is entered in units of the DOF.
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The `Camera image lag' is a camera specific parameter, defined by the number of images in the pipe line from the camera.
To make a precise auto focus this must be entered; in most cases 1, 2 or 3 images will give a good result.

The System Automation Tab 159

160 DeltaPix InSight - Manual and Help The frame rate oversampling sets how many images are analyzed within each DOF. Example1: If the value is 1, and the DOF is 100um, then the focus is checked for every 100um move of the focus axis. Example2: If the value is 4, and the DOF is 100um, then the focus is checked for every 25um move of the focus axis.
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The System Automation Tab 161
With some motor controllers, the best result is achieved by using a system timer to calculate the best focus position. If the system timer is not used, then the position must be continuously read from the controller (which sometimes is a problem!),
The result may be improved further by interpolating the focus slice distance this allows the auto focus position to be in between two sample positions. If this option is unchecked, the sample position with the maximum focus will be chosen as the best auto focus position.
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The System Automation Tab 163 The `Optimized fast mode' check-box allows for a faster auto focus, this will work best of the focus is not too far away. In any case the search is limited by the `Search Range' interval.
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164 DeltaPix InSight - Manual and Help This check box defines the mode of the one zone auto focus. If box is checked, then a flexible region is used. This zone must defined prior to running the auto focus. The region is flexible in position and size.
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If using the two zone auto focus, a top and bottom level is determined.
For subsequent operations, like extended focus or topography/3D, an extra margin can be added to the range - this is entered as a percentage of the distance from bottom- to top-level.

The System Automation Tab 165

166 DeltaPix InSight - Manual and Help If using the two zone auto focus, a top and bottom level is determined. When the bottom- and top-level is found, a pop up can be requested to show the levels before more (time consuming) processing.
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The System Automation Tab 167
The 'Star' button loads the default parameters.
15.4.4.1 One region Auto Focus The auto focus works alone or in combination with: Alone, just to focus the live preview image before capturing Before Extended Focus. This makes the need for very many z-slices less demanding. With Extended Field (Stitching) With XYZ- acquisition.
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168 DeltaPix InSight - Manual and Help Two modes is available, center weighted image or region. The mode is selected in the auto focus settings panel.

Tip: By Ctrl-Alt-Click on the
This for testing purposes only.

button - the the actual region is indicated in fake color.

15.4.4.1.1 Global center weighted Auto Focus
If using the global image auto focus, just click the auto focus button

15.4.4.1.2 One Region Auto Focus
To use the region auto focus the region must be defined prior to running the auto focus.

Click the

button, and click on the live preview image to position the region.

Click one time more to lock the position.

Then the region can be sized.

When region is defined just click the

button to run the auto focus.

The System Automation Tab 169

15.4.4.2 Two region Auto Focus To use the two region auto focus the regions must be defined prior to running the auto focus.

Click the

button, and click on the live preview image to position the bottom-level

region.

Click one time more to lock the position.

Do the same for the top-level region.

Then the region can be sized.

When region is defined just click the

button to run the two region auto focus.

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After the focusing, bottom- and top-level values are transferred to the Focus Automation panel then extended focus or topography/3D can be run with ease.
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The System Automation Tab 171
After two zone auto focus. The regions show the z-level values.
Note: If zones are defined, you can also simply click extended focus or topography buttons directly.
15.4.5 Making the motor speed relative to the DOF
Most motor controllers can be configured to make their focus speed relative to the DOF. In practice this sets the speed lower if the DOF is lower. As example a 25µ m DOF will run at 25% of the speed of a 100µ m DOF. The speed set in the focus controller panel will be used for the highest DOF. The DOF must be defined in the Optics Editor.
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15.5 Zoom Automation
The Zoom Automation is used for controlling a motorized Zoom objective.
15.5.1 Zeiss MTB
Note: The zoom automation is only available for Zeiss microscopes with motorized zoom. Click the up/down buttons to change the zoom factor, The actual magnification is displayed.
A specific zoom position can be memorized by the `Set' button.
NOTE: The Zeiss Zoom control is configured and connected with the focus control! NOTE: Requires a well configured MTB2011 server. See Installing and connecting to Zeiss MTB 2011
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15.5.2 DeltaStep4
The controller communicates via a virtual serial port. This is easily identified as a `Silicon Labs CP210x' device.
If this is not already automatically selected, select it and click connect.
When successfully connected, a green check mark is shown.

The System Automation Tab 173

IMPORTANT:
When using pre-calibrated optics - an objective with a objective description file (see section Optics Editor for more on this topic). The "Sweep Zoom Range at Startup" check box MUST be checked for proper initialization of the motorized zoom objective.

The tools drop-down shows the power supply of the controller. Both Running and standby current can be set.
The polarity of the limit switches can be set. To change the polarity check-box value, the shift key must be pressed while clicking this is to prevent unintended change.
Utmost care must be taken: Selecting the wrong polarity may damage your motor device!

If the controller has zoom knob control, then automatic read back of position can be enabled by checking the `Has focus knob' check box.

174 DeltaPix InSight - Manual and Help Backlash The backlash can be set, this may result in a more precise zoom value - especially when using the click stop values. The value is entered as full stepper motor steps, so the best value depends on the stepper motor and optional gearbox.
16 Accessories Tab
16.1 Zeiss microscope controls
DeltaPix InSight can control most motorized/encoded microscopes. Version 3.2+ can control or listen to:
Objective changer Tubelens turret changer Reflector changer The Reflected Light Shutter

In order to make calibration work, one trick is necessary:: You will also need to define the objectives in the optics editor and in the SAME order as in the "Objective" drop down menu. The order in the "objective" drop down is determined by the MTB2011. Then selecting another objective from this drop down, will also change the objective on the microscope.
NOTE: Requires a well configured MTB2011 server. See Installing and connecting to Zeiss MTB 2011

16.2

Mettler Toledo Hot Stage HS82
The Mettler Toledo Hot Stage HS82 can be controlled from DeltaPix InSight, a temperature profile can be defined. The Stage is connected via a serial port.

The temperature data is read once every second and the actual temperature can be rendered into the image bitmap in Celcius or Kelvin. So it should also be visible for a lapse time recording.

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16.3 PixLight Ringlight (Valid from InSight 6.4)
The DeltaPix PixLight ring light comes is different versions
White/Red 5 zones. White/Blue 6 zones with zone or single LED light White/UV 6 zones with zone or single LED light
The ring light capabilities can be read from the ring light - and the GUI updates accordingly.
Selecting color Click the color switch white or blue in this example.

Zone or single LED
If the ring light supports single LED light, then the Zone/Single switch is enabled.
Click the zone/single switch to select zone or single LED light
Using single LED light is essential when doing photometric.

Intensity The light intensity can be set by the Intensity slider.
Key switch Activating the (optionally) key switch will switch to the primary color and lock the color selector. The key switch is aimed to protect against UV light.

Accessories Tab 177

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16.4

Objective change scanning
By attaching a dot- or bar-code to each objective position, it is possible to make the objectives encoded in a smart way.

The associated calibration will automatically be applied.

Just below the bar code button, the most recent read code is displayed, in this example "<2>"
Objectives are indexed 1..N, and the label for e.g. objective #3 should contain the string "<3>". IMPORTANT: Objectives MUST be labeled in the same order as defined in the optics definition editor. Codes are easily created on-line from this page : https://barcode.tec-it.com/en/QRCode
Code for 1st objective "<1>"
Code for 2nd objective "<2>"
Code for 3rd objective "<3>"

Code for 4th objective "<4>"

Accessories Tab 179

Code for 5th objective "<5>"

Code for 6th objective "<6>"
16.4.1 Example: Askania 6Z
The Askania 6Z stereomicroscope does not have stepless zoom, but has 6 precise click stops: 1. 0.32x 2. 0.63x 3. 1x 4. 3.2x 5. 1.6x 6. 1x (physically the same as click stop 3)
This should be defined in the optics editor like:

Note: the magnifications get sorted in ascending order.
Since the code labels refers to the index in the optics editor, the code labels should be arranged in the following sequence:
1. <1> 2. <2>
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180 DeltaPix InSight - Manual and Help 3. <3> 4. <5> 5. <4> 6. <3>
17 Image Compare Valid from DeltaPix InSight v6.2.9
Images can be compared in a number of ways. The compare functions in many ways replicated the functionality of a traditionally `analogue' comparison microscope but much simpler and more flexible to use. Only one light setup is required no need to do tedious work to set up identical right and left sides. A reference image can be directly captured from the live camera image, or a stored still image can be set as reference. The `Compare Image' is located in the `Image Compare' tab.
Sequence to prepare a compare session: 1. Select the "Sample A". This can be a still or the camera live image. Optionally a live comparison
can be done, then select from the drop down. 2. Select the "Sample B". This can be a still or the camera live image. 3. Click the "Start Compare". This will spawn a new comparison image. 4. Different kinds of comparisons can be done, select from the drop down combo box.-
None - no comparison is done Difference - the images are subtracted. Alpha blend - the images are added, 50% from each. Horizontal - the images are split and shown side-by-side. Vertical - the images are split and shown side-by-side.
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Example: Vertical side-by-side. Use the split slider to place divider.
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Example: Difference, one image is inverted Aligning the Sample B. The sample B can be aligned using the Angle, DX and DY sliders. Note: The DX and DY range can be extended by Alt and Ctrl modifier keys:
the Alt Key extends by a factor of 2 the Alt Control extends by a factor of 2 the Alt+Ctrl Key extends by a factor of 4 Alternatively the sample B can be aligned using the mouse. Use the mouse wheel to rotate. Push the mouse button and drag the image.
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Comparison options.

Image Compare 185

The Sample A and B can be swapped
The "Setup" button defines a remapping function it applies to both the live and the reference image.
This can enhance e.g. the evaluation of difference comparisons.

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18 Topography [from v6.4.0]

DeltaPix InSight can create 3D surface from a Z-stack of images.
High-precision topography measurement using focus techniques can be realized provided that the in-focus plane of an imaging system is very accurately determined.
To create surface charts, it is highly recommended to use medium resolution images, ~2 MP images are sufficient. More pixels will slow down the processing, and potentially limit the number
of images in the Z-stack

The topography processing and display requires a powerful computer to ensure fast creation of

images and smooth display.

Precision of the topography Precision can be better than 1 micron, dependent on optics magnification, focus motor precision and light quality. Several factors influence the precision:
1. A low depth-of-focus (DOF) is desirable. High magnification and numerical aperture generally decrease the DOF.
2. The number of focus slices acquired for processing. If many slices are used the better precision. As a rule of thumb, the slice distance should be less than the DOF, around 1/2 DOF to 1/4 DOF is recommended.
3. The best Z resolution is 1/250 of the Z used travel range, so if the difference between top and bottom level is 1mm, then the very best precision is 1000um/250 = 4um.
4. The precision is also limited by the number of Z-stack images used. As worst-case example, if only 3 images are used, and the difference between top and bottom level is 1mm, then the precision is 1000um/3 = 333um. The result may look better since a configurable interpolation and noise reduction between the Z-stack layers is done.
5. The surface of the object. Shiny or black surfaces generally are difficult to handle.
6. The precision of the mechanics primarily the focus motor. This is however normally in the um-range.

Topography [from v6.4.0] 187
18.1 File
The topography offers several ways of saving, opening and copying 3D data.
Print A topographic 3D view can be printed to any type of standard printer. Realize Realizes the topographic image currently shown. The 3D surface or 2D profile image will be saved as a 2D image. This image can be viewed in most graphics programs. Copy Copies the 3D surface or 2D profile image currently shown to the clipboard as a realized 2D image. This image can be pasted into most graphics' programs. The chart can then be inserted in e.g. a Microsoft Word document.
18.1.1 Open
Open previously saved topography images, 3D surface or 2D profile.
The bin-format is a proprietary binary format.
Zeiss
An image stack acquired from a Zeiss microscope (e.g. using Zen), can be opened and processed to a topographic 3D image.
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18.1.2 Save
3D images can be saved in a variety of file formats.
Topographic 3D images can be saved as comma-separated-values, plain ASCII text, or proprietary binary format. Alternatively, the 3D image can be saved in a Digital Surf compatible format, for further analysis in the Digital Surf Mountains Map software,
TXT format Optionally the surface data can be exported as a flat CSV or TXT file.
The file format is proprietary but can easily be parsed by 3rd party software. The format is <indexX>, <indexY>,<ValueX>, <valueY>, <valueZ>, <red>, <green>, <blue>, <alpha>
//-----------------------------------------------------------// DeltaPix InSight // Chart has 160 x 106 datapoints. // Unit XY is 0,011mm / point // Unit Z is µm // Saved: 20-10-2019 09:41:30 //-----------------------------------------------------------0000; 0000; 00000,000; 00000,000; 00003,063; 145; 125; 156; 255 0001; 0000; 00000,011; 00000,000; 00003,063; 144; 129; 163; 255 0002; 0000; 00000,021; 00000,000; 00003,063; 125; 126; 150; 255 0003; 0000; 00000,032; 00000,000; 00003,063; 153; 134; 153; 255 0004; 0000; 00000,042; 00000,000; 00002,784; 136; 131; 145; 255 0005; 0000; 00000,053; 00000,000; 00002,784; 136; 123; 148; 255 0006; 0000; 00000,063; 00000,000; 00002,506; 138; 119; 152; 255 0007; 0000; 00000,074; 00000,000; 00002,506; 138; 123; 155; 255 0008; 0000; 00000,084; 00000,000; 00002,227; 144; 131; 163; 255 0009; 0000; 00000,095; 00000,000; 00002,227; 143; 137; 163; 255 0010; 0000; 00000,105; 00000,000; 00001,949; 150; 133; 158; 255 0011; 0000; 00000,116; 00000,000; 00001,671; 141; 137; 156; 255
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Topography [from v6.4.0] 189 Stereolithography
To export the topographic 3D surface to a 3D printer, the stereolithographic STL file format can be used.
The STL file can have side walls added, and optionally automatically opened in the default STL application after creation. Workspace Different topographic workspaces can be saved, this makes the switch from one topographic analysis type to another easy.
Save your topography workspace for a specific analysis, and simply load it next time the same task needs to be done.
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18.2 Topography
The topography 3D creation can either be done by acquiring Z-stack images manually, or fully automated using a motorized focus microscope.

Manual topography mode

Automatic topography mode

18.2.1 Manual Topography
It is possible to create a topographic image without motorized Z axis but to have a good image the manual capture must be done equidistant at the Z axis.

To have the right scaling, the top and bottom level must be entered. Note: that if both top- and bottom-level is set to 0, then an automatic scaling will be done.
i
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Topography [from v6.4.0] 191
18.2.2 Automatic motorized Topography
Performs an automatic image capture and topographic processing. The number of images can be set up manually or calculated from the requested slice distance.
By checking the `Show Extended Focus Image' check-box, the Extended Focus image is shown after the topographic processing.
18.2.3 Use Z range from previous topography This function can be used to narrow down the top and bottom positions, starting from a rough setting.
Click `Yes' to use the suggested range, `No' to continue, but not use the suggested range. Click `Cancel' to skip the creation of a topographic image.
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The function will use the topographic top and bottom levels and add a margin of 2 x 12.5%.
18.2.4 Parameters
Quality Select the quality. Normally `Good' quality is recommended. Please note that the better quality, the better/faster computer is required! Spike remove filter This parameter determines the strength of which height noise (but also image details!) is filtered away. A larger value will give a smoother surface. Step Smooth Strength This parameter determines the strength of which the height map is smoothed. A larger value will give a smoother surface. Radius Sets the radius within which to compare focus, to calculate the Z height. Radius is the control that sets the size of the analyzed area around each pixel.
Try different values. Start with a small value to see the result. Then, stepwise increase the value to remove noise, unwanted artifacts, or halos along the edges. For scenes with `small' details a low radius of about 3-5 will produce the good results.
18.3 Compensation
Optical compensation
Skip boundary The boundary of the 3D plot may have weird bends due to the difficulties of determining the right focus plane. In such cases the 3D may show up better if the boundary is slightly trimmed. Suggested value is 3-5% Focus parabola
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Topography [from v6.4.0] 193 The boundary of the 3D plot may have weird bends due to the difficulties of determining the right focus plane. This is often due to non-telecentric objective or shift in a stereo microscope. In such cases the 3D may show up better if the boundary is slightly trimmed away. Suggested value is 3-5% Mechanical compensation When making 3D plots of surfaces with very little height, it is very disturbing if the stage has a slight tilt. This may be corrected by the X- and Y-tilt compensation.
18.4 3D View
The entire chart can be zoomed using the XY or Z +/- magnifier buttons. Color scheme Select an appropriate color. Most often the `Real Color' option will be used as this reproduces the real object most naturally. If the `Zone' color is used, then the height can be read from the color legend. Each color in the legend corresponds to an input image, also representing the DOF. Walls
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Part of the Berlin Wall shown with Walls
Contour lines The `Contour' option will show the image layers.
Projection Two options exist, perspective or orthogonal view.
Light Several artificial light models can be applied to change the contrast. For the `Real Color' view, the `None' option is recommended.
Text Header and footer texts can be added. Annotations can be added by right clicking on a position on the 3D surface. The callout will contain XYZ-information and the text in the `Callout Text' text-box. By mouse pointing the 3D line chart, the XYZ data is shown.
To add custom text to an annotation box, first select the annotation by clicking it with a mouse button. When selected, the color will change. Then type the text in the textbox.
Flip Surface This option inverts the surface, like it is seen from below. Also, useful if e.g. the 3D surface Z-stack was acquired in the wrong direction.
18.5 3D Measuirement

Region

Topography [from v6.4.0] 195
The wall color can be changed to enhance visibility. The narrow/widen buttons can be used to adjust the region.
The best way to move a region is by mouse dragging the control bubbles. The control bubbles automatically show up when the mouse is moved within the 3D chart. The bubble color is always a `complementary' color to the region color.
The 4 corner points control the width and length of the region. The 2 midpoints control the length and angle of the region. The center point controls the overall position. Remove Curvature This feature can remove smooth large-scale variations. This is useful when doing e.g. roughness measurements on a curved surface.
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The curvature removal uses a dedicated filter. The size of the filter can be set:
If set small also small variations will be flattened away. If set large, only large variations will be flattened away.

Note: The boundary of the 3D image will become invalid when using this feature. Using a

small value will only make a small fraction invalid, bigger values will make the invalid

boundary bigger.

The `Skip boundary' parameter will automatically be updates to hide invalid boundaries.

A value of ~5% is suggested for typical use.

The kernel size should be significantly larger than the 'roughness' to be measured. Note: Focus parabola and stage tilt compensation should be set equal to 0.

18.5.1 3D Roughness
Roughness The Surface Roughness implements a subset of ISO 25178
Height and volume parameters can be selected via the drop-down check boxes.

The volume parameter calculation offers settings for the mr1 and mr2 input parameter.
The parameters are associated to a topographic 3D image - data shown in a panel in the lower right corner.

Sample roughness date NOTE: When clicking the "Surface Roughness" button, the data is also copied to the text clipboard. NOTE: When clicking the "Surface Roughness" button, the data is also copied to the text
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clipboard.

Topography [from v6.4.0] 197

18.5.1.1 Abbot Firestone
By pressing 'Shift' simultaneously clicking the "Roughness" button, the Abbot Firestone curve will be shown.

Example of Abbot Firestone curve
The Abbott-Firestone curve or bearing area curve (BAC) describes the surface texture of an object. The curve can be found from a profile trace by drawing lines parallel to the datum and measuring the fraction of the line which lies within the profile. Mathematically it is the cumulative probability density function of the surface profile's height and can be calculated by integrating the profile trace.
18.6 2D View
The 2D view offers a few options.
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2D Min-Max The 2D profile data is an average of the values inside region. The display of min/max adds two curves to the 2D profile - the minimum and the maximum values used for the average calculation. 2D True Color The 2D profile can be displayed as a plain line curve. Alternatively, the 2D profile can be displayed using its real color, as shown on the 3D surface plot. Texts Header and footer texts can be added. Annotations can be added by right clicking on a position on the 2D profile. The callout will contain XYZ-information and the text in the `Callout Text' text-box. By mouse pointing the 3D line chart, the XYZ data is shown. To add custom text to an annotation box, first select the annotation by clicking it with a mouse button. When selected, the color will change. Then type the text in the textbox.
18.7 2D Measurement
Region

The wall color can be changed to enhance visibility. The narrow/widen buttons can be used to adjust the region.

Note: that if both top- and bottom-level is set to 0, then an automatic scaling will be done.

Topography [from v6.4.0] 199
The best way to move a region is by mouse dragging the control bubbles. The control bubbles automatically show up when the mouse is moved within the 3D chart. The bubble color is always a `complementary' color to the region color.
The 4 corner points control the width and length of the region. The 2 midpoints control the length and angle of the region. The center point controls the overall position. Mouse wheel control Optionally the 2D region can be modified by the mouse wheel + a modifier key: Wall X position: Mouse Wheel + Shift Wall Y position: Mouse Wheel Wall Length: Mouse Wheel + Ctrl + Alt Wall Separation: Mouse Wheel + Ctrl Wall Angle: Mouse Wheel + Alt If the walls will extend beyond the 3D curve, then they will be truncated.
18.7.1 2D Roughness The Surface Roughness implements a subset of ISO 4287 Clicking the Roughness button will enable live update of roughness data when moving the region. The parameters are associated to a 2D profile - data shown in a panel in the lower right corner.
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Sample 2D roughness date
18.7.2 2D Profile Measurements
Measurements can be done on the 2D profile. The 2D profile measurements can be exported into a report, please refer to the reporting section. Height
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Topography [from v6.4.0] 201
The region can be dragged by mouse. The height difference is shown. Angle The two regions can be dragged by mouse. The angles relative to horizontal is shown.
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Circle fit The region can be dragged by mouse. The best circle fit of XY data within the region is shown.
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Topography [from v6.4.0] 203
18.8 Reporting Reporting
A report can be generated in Excel format. This allows for further processing in the spreadsheet. The reporting uses an Excel template, defining the layout of the report. Image, measurement results and other information is placed in the spreadsheet by identifying tags.
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To make distribution very easy and flexible, a report can be published as PDF.
18.8.1 Template A template must be defined, click the Template button to navigate to a template. After a template has been defined, simply click the Excel report button. Valid tags for 3D roughness data are: %IMGS% defines the upper-left corner of image. %IMGE% defines the lower-right corner of image. %MEAS% defines column for measurement results. %UNIT% defines column for measurement units. %COL% makes a color marker in the color of the annotations text color.
Valid tags for 2D roughness data are: %IMGS2% defines the upper-left corner of image. %IMGE2% defines the lower-right corner of image. %MEAS2% defines column for measurement results. %UNIT2% defines column for measurement units. %COL2% makes a color marker in the color of the annotations text color.
Valid tags for 2D measurement data are: %IMGS% defines the upper-left corner of image. %IMGE% defines the lower-right corner of image. %MEAS% defines column for measurement results. %UNIT% defines column for measurement units. %COL% makes a color marker in the color of the annotations text color.
General tags are: %DATE% defines date. %TIME% defines time. %CAMERAMODEL% defines camera model. %PICTUREDPI% defines the image DPI.
19 Video recording [from v6.2.9]
The video recorder allows for real time recording or lapse time recording. The recording- and the playback-frame rate can be set independently.
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Video recording [from v6.2.9] 205

Optionally an overlay text can be specified, this can contain actual recording- and playback time.

NOTE: THE OVERLAY TEXT IS CURRENTLY UTF-8 WHICH MEANS THAT SPECIAL CHARACTERS WILL IS NOT SUPPORTED. THE HERSHEY FONT USED ONLY HAS A LIMITED SUBSET OF ASCII. IT WON'T SHOW ACCENTS, CIRCUMFLEX OR ANY "SPECIAL" CHARACTERS LIKE THAT. SPECIAL CHARACTERS ARE CONVERTED TO NEAREST EQUIVALENT.

19.1

Recording frame rate
The recording frame rate can be set to anything fixed from 1 frame-per-hour to 50 frames-persecond. Alternatively, the actual camera frame rate can be selected.

Panel for setting the recording frame rate.
Maximum frame rate The recording frame rate can never be higher than the actual camera frame rate. The actual camera frame rate can be read in the camera panel.

In this example the actual camera frame rate is 61fps, but the display frame rate is limited to 30fps.

19.2

Playback frame rate
The play frame rate can be set in the range from 1 100 frames-per-second. Alternatively, it can be locked to the recording frame rate.

Panel for setting the recording frame rate.

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19.3 Recording time
The recording time can be set anywhere in the range from 0h:0m:1s to 999h:58m:59s.

Panel for setting the recording time. The "No time limit" check box enables endless recording, which will require a manual stop recording
19.4 Information
An information string can optionally be embedded into the video

Panel for setting the optional embedded information.
The above example "Recording time : %T%" string will embed something like "Recording time : 00:01:23" as the %T% tag will be replaces by the recording time.
%d% will insert actual date (MM:DD;YY). %t% will insert actual time (HH:MM:SS). %T% will insert recording time (HH:MM:SS), starting from 00:00:00 %P% will insert playback time (HH:MM:SS), starting from 00:00:00

Example: "Date:%d% Time:%t% Rec:%T% Play:%P%" will show up like: "Date:07/09/20 Time:08:33:58 Rec:00:00:15 Play:00:00:01" The position is in pixel coordinates, (0.0) is top-left corner.

19.5

Recording
First a file name must be assigned to the video. Then the recording is started using the "Record" button

Panel for controlling the recording.

The recording can be paused at any time. The recording can be stopped at any time.

Video recording [from v6.2.9] 207

An ongoing recording is indicated by a green time string showing the recording time. This will turn orange when the recording is paused.
The Play button will play a video using the standard video player. If possible, the recording time and the play time is show in the bottom line. The recording time and play time may be different when recording and play frame rate differs.
Pause preview while recording When recording at high frame rate, it may be necessary to save some computational resources by pausing the preview. This is done by the record drop down check box.

Setting the pause preview.
Other ways to save resources Instead of completely pausing the preview, the preview frame rate can be reduced.

20 Shortcuts and special Keys
Many functions can be activated using keyboard shortcuts. Function Close all images Capture Image

Shortcut Ctrl-Shift-C F1 Ctrl-Shift-I (hotkey)

208 DeltaPix InSight - Manual and Help
Close live preview Save CSV file (or append measurement data) Bold Italic Underline Paste Delete Copy Insert calibrated grid Open XYZ control panel Manual Extended Exposure Automatic Extended Exposure Insert Scale Bar Manual Extended Focus Automatic Extended Focus Automatic Extended Exposure and Focus Force Image Red Force Image Green Force Image Blue Open Image Print Image Remap Intensity Open Optics Editor Pause preview Full Screen Toggle monitor. Toggles between all attached monitors. Open Control Panel

Shift and click X F12 Ctrl-B Ctrl-I Ctrl-U Ctrl-V Del Ctrl-C Ctrl-G Ctrl-F12 F5 Alt-F5 F8 F6 Alt-F6 Shift-Alt-F6 Ctrl-Shift-R Ctrl-Shift-G Ctrl-Shift-B Ctrl-O Ctrl-P Ctrl-Alt-R Shift-Alt-O Alt-F1 F11 (hotkey) Alt-F11 Shift-Alt-L

Shortcuts and special Keys 209

Capture flat field reference image (Shading correction) Enable flat field correction (Shading correction) About Undo (not all operations supported) Save Image As Undo processing Toggle auto exposure on/off
Point white balance Global white balance Default white balance Create a new measurement, same type as the most recently used. Toggle tabbed - MDI View Tile Vertical (when in MDI view) Tile Horizontal (when in MDI view) Arrange (when in MDI view) Launch 'Quick System Setup' tool Manual Topography Save All (Gem alt) Select Easy save directory Auto Stitch start XYZ table start acquisition

Shift-Alt-S Ctrl-Shift-S Shift-Alt-A Ctrl-Z Ctrl-S Ctrl-Shift-Z Alt-F2 Ctrl-Shift-J Alt-F9 Alt-F10 Alt-F11 Spacebar
Ctrl-Shift-T Ctrl-Shift-V Ctrl-Shift-H Ctrl-Shift-A Ctrl-Shift-W Ctrl-Shift-A Alt-Shift-G Ctrl-E Alt-Shift-Y Alt-Shift-X

20.1 Special start-up modifier keys

In some cases it can be necessary to launch the DeltaPix InSight in special or safe mode.

Function Reduce the the frame rate to ½x

Key Alt

Comment Press immediately after program launch -

210 DeltaPix InSight - Manual and Help default

Disable all motor control Avoid workspace load

Shift Ctrl-Alt

Launch a debug window

Ctrl

pressing before, may open the programs Properties dialog.
Keep the key down until program has completely started.
Keep the key down until program has completely started.
Press immediately after program launch pressing before, may open the programs Properties dialog.
Keep the key down until program has completely started.
Keep the key down until program has completely started.

20.2 Special shut-down modifier keys

Function Avoid workspace save

Key Ctrl-Alt

Comment
Keep the key down until program has completely stopped.

20.3 Motor speed modifier keys

In some cases it is convenient to run the XYZ motors at another speed than the pre-programmed speed, or the speed calculated from the Magnification or DOF. By activating modifier keys as listed in the table, the speed can be increased or decreased.

Modifier Key

Ctrl

Alt

Shift a

Action
No modification - relies on settings (Speed, Magnification, DOF)
2x speed

Shortcuts and special Keys 211

1/2x speed

1/4x speed

Full speed as set in motor configuration

4x speed

1/8x speed

8x speed

21 FAQ - Frequently Asked Questions
21.1 Software freeze checklist
Our general trouble shooting list for freezing software or non-responding cameras is:
1. USB cable must be high quality, and as short as possible, not more than 2mtr. USB cable problems during time can show up as black images, non-responding cameras or other ways that not at first sight look like USB cable problems. Try another short good quality cable.
2. Do not connect the camera via an external USB hub. 3. Some computer build-in USB ports operate via an internal USB hub. Often, but not
always, the ports on the front are using an internal USB hub. Try another port. 4. Some new computers try to save power by managing the power, powering down a USB
port may cause the attached hardware to malfunction. See section 'Fix an Annoying Windows USB Power Problem' on how to avoid this. 5. Carefully avoid exposure to static electricity. Static discharge may cause the camera to freeze or malfunction. 6. Disable the internet connectivity. DeltaPix InSight may look for software updates, but your company security can block this, and cause the software to `hang'. As a test, disable the internet connection on the computer (disable Wireless, or remove cable). The Internet connectivity can also be disabled within the InSight software. 7. The software will not run on a `Guest' account avoid this. 8. The USB plug of the camera may become `loose' and unstable during time, especially on systems with frequent cable plug/unplug. 9. If the camera is a USB3 camera, try it on a USB2 port. 10. Is the camera a InvenioIII camera - like e.g. Invenio 5SIII or Invenio 6EIII , then check that the green LED is NOT blinking. 11. Check the communication to external devices. For serial COM port communication, the

212 DeltaPix InSight - Manual and Help
driver version is essential

21.2 Installing Prior software
The installed Prior software should be 8.5.2.x64 or 8.5.4.x64 This is available from www.prior.com or from www.deltapix.dk
21.3 Understanding magnification
Magnification is a matter of definition.
Normally we think of magnification on a digital microscope as the ratio of the shown size on the PC monitor compared to the actual physical size. So if the object has a dimension of 1mm and displays on the monitor as 12.3cm, then the magnification is 123x (So also the monitor size and resolution is to be considered).
In short we calculate the magnification as:
Mt = Mo x Md x PSm / PSc;
Where: Mt is the total magnification Mo is the optical magnification Md is the digital magnification (like if you zoom using the mouse wheel...) PSm is the pixel size on the monitor (You need to set you monitor size in the DeltaPix InSight control panel, resolution can be read by the software). PSc is the pixel size in the camera.
Please consult the manual, section "Show Absolute or Relative magnification".

21.4

Get rid of old Invenio 3S/5S drivers
If you are updating from DpxViewPro (discontinued 2009) to DeltaPix InSight then it may be slightly difficult to install the new drivers:

To get rid of the old driver for the Invenio 3S do the following:

1. Detach the camera Invenio 3S 2. Goto the C:\windows\inf directory 3. Delete files with name X18cu.inf and X18cu.pnf 4. Goto the C:\windows\system32\drivers 5. Delete files with name X18cu.sys 6. Attach the camera Invenio 3S 7. Make the device manager look for a new driver.

FAQ - Frequently Asked Questions 213
21.5 Install driver for For InvenioIIIeb Cameras
Installation of (1st generation only) Invenio-5SIII and Invenio-10SIII device drivers Do not attach the camera yet. Install the DeltaPix InSight Follow this procedure to manually install device drivers for the Invenio-5SIII and Invenio-10SIII cameras.
Launch the Setup_InvenioIIIeb_a.b.c installer Note: a.b.c is the version number of the installation program, and may change.
Click next to start the installation.
Click 'Install' to install the device software.
© 2022 DeltaPix Support

Click 'Finish'.

FAQ - Frequently Asked Questions 215

Click 'Finish'. Now attach the camera to a vacant USB3 (or optionally a USB2) port. Is is NOT recommended to connect via a USB hub. Let the camera install - may take a few minuttes.
The camera should now appear in the Device Manager as Invenio-5SIII or Invenio-10SIII
© 2022 DeltaPix Support

216 DeltaPix InSight - Manual and Help If the camera does not install correctly, please contact support@deltapix.dk
21.6 Disable Internet connectivity
If your company network is very restrictive - it may be necessary to disable the internet connectivity to avoid a freezing program To disable all internet activity, make sure that the 'Check for news' and 'Check for software updates' check boxes are unchecked.
© 2022 DeltaPix Support

FAQ - Frequently Asked Questions 217

21.7

Frame rate drops
The camera preview display may use a major part of the computers resources. DeltaPix InSight contains several mechanisms to balance the frame rate to release computing resources.

This may in some rare cases lead to a (annoying) reduced frame rate.

The frame rate can be forced to maximum by right clicking the "Live Image" button.
21.8 Fix an Annoying Windows USB Power Problem
Since XP, the Windows operating system has come with a feature called USB Selective Suspend. This allows the system to turn off specific USB ports without affecting the other USB ports. This is supposed to save power by closing down ports that are only used intermittently.
However, sometimes the port does not get turned back on when it is needed. On a desktop or other system where this type of power saving is not crucial, consider disabling this feature if you are experiencing USB connection problems.
The feature can be disabled or re-enabled in Control Panel. 1. Open Control Panel 2. Go to: Hardware and Sound->Power Options 3. Find your preferred power plan and click "Change plan settings" 4. Click "Change advanced power settings" 5. A dialog box similar to the example in the graphic will open 6. Find the entry "USB settings" and expand it 7. Expand the entry "USB selective suspend setting" 8. Use the drop-down menu to disable or re-enable the setting as shown in the graphic below 9. Click "OK"

218 DeltaPix InSight - Manual and Help
In Windows 8.1, the problem of a USB port being turned off can be even worse. See this MSDN post for details. Incidentally, the problem of an unresponsive USB port can also often be fixed by turning the computer off and back on. Thanks to: http://www.techsupportalert.com/content/how-fix-annoying-windows-usb-problem.htm
21.9 ATEN USB to RS232 converter driver
Incorrect driver versions may cause the program to freeze or crash. Always make sure that you use updated drivers for your converter. For Windows 8/8.1 (64/32), 7 (64/32), Vista (64/32), 2003, and XP the following driver should is recommended: CP210x USB to UART Bridge Driver v6.6.1 - October 24, 2013 This should look something like this in the device manager.
© 2022 DeltaPix Support

FAQ - Frequently Asked Questions 219

21.10 USB Cables
USB ports and connectors are often color-coded to distinguish their different functions and USB versions. These colors are not part of the USB specification and can vary between manufacturers; for example, USB 3.0 specification mandates appropriate color-coding while it only recommends blue inserts for Standard-A USB 3.0 connectors and plugs.

USB 1.x USB 2.0 USB 3.0 Sleep-and-charge

White Black, sometimes white Blue Yellow or red

21.11 Graphics performance
By selecting a Power Plan for your computer you may affect the graphics performance.
It is not recommended to use the 'Power saver' plan, as this may disable the GPU.´, and slow down camera frame rate and 3D performance.

220 DeltaPix InSight - Manual and Help
21.12 A generic error occurred in GDI+
This error is a common one and also very tricky. The error itself is not very descriptive. Permission problems is the common cause, but there have been some cases where the permissions are fine and it still happens. If this error is caused by permissions then there are two possible scenarios:
The account the process is running as does not have permission to write images to the target directory. Fix your permissions. The target directory does not exist. Create it first. It may be difficult to tell exactly which directory or permission is invalid.
22 Appendix
© 2022 DeltaPix Support

Appendix 221
22.1 Backup - Restore - Cloning
Creating a backup
When a system is fully configured, it is recommended to do a backup of the complete settings. This backup can be used for:
To restore the settings on the same computer in case of emergency. To clone the settings to another computer or another user on the same computer. Click `Backup Configuration'
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222 DeltaPix InSight - Manual and Help
Select a folder for the backup. It is preferable to use an empty folder, eventually make a new folder. Click OK
Files will be copied, and a message pops up when complete. Note: while copying files, the program might seem unresponsive, so please be patient while the copying is taking place.
Restore Settings or cloning to another computer.
To clone the settings, use the `Quick System Setup' wizard. This can be launched at any time from the control panel, or by the Ctrl-Alt-W keys.
© 2022 DeltaPix Support

The Clone option is the 2nd step in the `Quick System Setup' wizard.
Click `Clone Now'.

Appendix 223

Click `Yes' if you are sure that you want to do the cloning. NOTE: There is no possible undo to a cloning!
Select the folder containing the files to be used for cloning. All necessary files were generated from a `Backup Configuration', as described elsewhere. Click OK, to start the cloning.

224 DeltaPix InSight - Manual and Help
Files will be copied, and a message pops up when complete. The program will close, and will need to be launched again manually.
© 2022 DeltaPix Support

Appendix 225

22.2

External Drive for Settings
Program settings can be automatically stored on a specific drive, e.g. a removable USB drive. This feature makes system distribution easy, and eliminates the need for cloning.

The drive is identified by it's volume label. The volume label Must be: DELTAPIX_SETTINGS

The drive should be formatted as NTFS.
There are several ways to put the settings onto the external drive: 1. Insert the DELTAPIX_SETTINGS drive before configuring the DeltaPix InSight
a. Settings will then automatically be stored on the drive when closing DeltaPix InSight 2. Insert the DELTAPIX_SETTINGS drive after configuring the DeltaPix InSight - follow Method 1
or 2. NOTE: The settings on the external drive are shared among all users.
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226 DeltaPix InSight - Manual and Help
22.2.1 Export Settings to External Settings Drive - Method 1
Enter topic text here.Follow these steps carefully to export settings data to an external settings drive. (1) Do NOT insert the external target drive yet! (2) Start DeltaPix InSight (3) Insert the external target drive, Make sure that the drive has volume label DELTAPIX_SETTINGS. (4) Prepare copy process by clicking "Open Application Data Directory".
(5) This will open the application data directory. Then: Go one directory up - to the Roaming directory. Select the DeltaPix directory. Right click this directory In the pop-up, select "Send to" In next pop-up select DELTAPIX_SETTINGS
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Appendix 227
(7) The copy process normally takes a few seconds - have patience. (8) Restart DeltaPix InSight - now the settings on the external drive will be used.
22.2.2 Export Settings to External Settings Drive - Method 2
Follow these steps carefully to export settings data to an external settings drive. (1) Do NOT insert the external target drive yet! (2) Start DeltaPix InSight (3) Insert the external target drive, Make sure that the drive has volume label DELTAPIX_SETTINGS. (4) Prepare copy process by clicking "Create Clone Files".
© 2022 DeltaPix Support

228 DeltaPix InSight - Manual and Help
(5) Browse to the DELTAPIX_SETTINGS drive.
(6) Create a directory named DeltaPix - using the exact name is vital. Select the newly created directory. Click OK.
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Appendix 229
(7) The copy process normally takes a few seconds - have patience.
(8) Restart DeltaPix InSight - now the settings on the external drive will be used.
22.2.3 Import Settings from External Settings Drive
Follow these steps carefully to import settings data from an external settings drive. (1) Do NOT insert the external source drive yet! (2) Start DeltaPix InSight (3) Insert the external source drive, (4) If the "Quick System Setup" wizard does not pop-up automatically. The press the "Ctrl-Shift-W" keyboard combination. (5) Go to the Clone page, and click "Clone Now".
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230 DeltaPix InSight - Manual and Help
(6) Browse to the external source drive (which in this example is named DELTAPIX_SETTINGS). Select the DeltaPix directory (which in this example is the parent directory for the settings). Click OK.

(7) After files are copied, a message box will pop-up. Click OK to close DeltaPix InSight

Appendix 231 (8) Remove the external source drive. (9) Start DeltaPix InSight - now the imported settings will be used.
© 2022 DeltaPix Support

232 DeltaPix InSight - Manual and Help
22.3 Installing and connecting to Prior (OptiScanII)
22.3.1 Installation
The OptiScanII does not have a full USB implementation so there is very little advantage in using it. Where no RS232 com port is available on the computer, we suggest using a USB to RS232 converter and then connecting to RS232 on the converter. This is to our best knowledge only a problem in conjunction with the Prior OptiScanII, and does not apply to e.g. the ProScan series and the ES10Z. Always consult the Prior documentation before installing Prior software.
22.3.2 64bit operating system
On a computer with a 64bit operating system
Install the 32bit version of the Prior software. If installing any drivers for the USB, install 64bit drivers. 22.3.3 Prior OptiScanII Z-motor operation.
In case the Z-motor spins at a different (like much lower) speed than expected, it might be the steps/mm parameter that needs to be changed to a lower value see below.
© 2022 DeltaPix Support

22.4 TWAIN
A 32 bit TWAIN source is available for InvenioII cameras. A 32 and 64 bit TWAIN source is available for InvenioIII cameras.

Appendix 233

Example of TWAIN capuure using an Invenio-6EIII camera.
22.5 DirectX (DirectShow)
DirectShow filters are available for the InvenioII and InvenioIII cameras. Note: These filters are not installed by the default installer.
22.6 Matlab
A Matlab camera driver is available for the InvenioIII cameras. Note: This driver is not installed by the default installer.
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234 DeltaPix InSight - Manual and Help
22.7 Installing and connecting to Zeiss MTB 2011
The Zeiss MTB2011 needs to be installed on your computer. Recommended steps for "DeltaPix InSight":
Use a clean Windows 10 computer without other security software that Microsoft Defender. Use the MTB2011 recommended for the actual version of DeltaPix InSight Install the MTB2011 RDK Used the MTBConfig to autoconfigure the microscope. Make sure that you have a DeltaPix dongle that supports the Zeiss microscope options. Follow the instructions to calibrate and set-up the microscope in DeltaPix InSight.
22.7.1 Using another version of MTB2011 than the default MTB2011 version The default MTB2011 version The default MTB2011 version is shown in the interface panel.
In the above panel, the MTB2011 v2.9.0.14 is the default version. If you want to use another MTB2011 version (MTB2004 cannot be used!), a version redirection must be done. NOTE1: The version redirection must be done every time the DeltaPix InSight is installed, as
© 2022 DeltaPix Support

Appendix 235
the installation reverts to the default MTB2011 version. NOTE2: The interface panel will still show the default MTB2011 version after a redirection has been done, it does not show the redirected version.
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236 DeltaPix InSight - Manual and Help
Run the InSight_RedirectMTB2011.exe redirection program
In the DeltaPix InSight installation directory run the InSight_RedirectMTB2004.exe program as administrator -right click on the file; select `Run as Administrator'.
Select the version of your MTB2011.
Select your version of MTB2011, by changing the green up-down text boxes. Then `Save'.
© 2022 DeltaPix Support

Appendix 237
22.7.2 Optics Editor and calibration hints
(1) The MTBConfig of the objectives must be the same as in the Optics Editor!
Example of a AxioImager M2 configuration. The x5 is calibrated and interpolated to the other objectives.
(2) The calibration must be done with a 1x Tubelens and a 1x Reflector magnification!
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238 DeltaPix InSight - Manual and Help
Example of a valid calibration setup. Tubelens is 1x and Reflector is 1x.
22.8 Märzhauser TANGO
DeltaPix InSight can operate a Märzhauser TANGO controller. Install the Märzhauser TANGO software as described by Märzhauser. Before connecting, the appropriate COM port must be selected.
© 2022 DeltaPix Support

Appendix 239
From the above `Computer Management' panel, it can be seen that the Märzhauser TANGO is connected via COM3. Select the right COM port, and click the connect button. Always consult the Märzhauser documentation before installing Prior software.
22.9 Mitutoyo MF - Communication Setup
Set up the microscope RS-232 communication to: Before DeltaPix InSight v6.1.18 : 9600, N, 8, 1, NoFlowCtrl . From DeltaPix InSight v6.1.18 : 9600, E, 7, 1 NoFlowCtrl (This is almost the default microscope setting)
See technical document 99MBA091A6 (31.05.2013) on how to configure the microscope. See page 1-11 on how to setup RS232 communication See page 1-13 on how to enter/exit setup
F07 Microscope Communication Setup F07-1 = 9600 baud
© 2022 DeltaPix Support

240 DeltaPix InSight - Manual and Help F07-2 = 7 databits F07-3 = Even parity F07-4 = 1 stopbit F07-5 = Flowcontrol (xon/xoff) = OFF F07-6 = Flowcontrol (hw) = OFF
F03 Resolution setup All axes, select the .1 Minimum readout of 0.1µm/0.00001" RS232 Cable
Optionally use a USB-RS232 converter in the PC end. Dataformat example Query : "A"<cr><lf> Response : "X +004.1880,Y +004.5870,Z +006.1523"<cr><lf> will display:
22.10 Custom Camera Configuration
The factory camera configuration can be overridden, to have customer specific camera properties. This is done via a dedicated file "FactoryCameraCfg_UsrDef.txt" that must reside in the Camera application data directory (something like "C:\Users\MyName\AppData\Roaming\DeltaPix \Camera"). This directory is easily opened by the "File | System Maintenance | Open Application Data Directory" button. Placing the file there makes it part of the backup-clone mechanism.
© 2022 DeltaPix Support

Appendix 241 To override e.g. the Invenio 6EIII - copy the file from the "FactoryCameraCfg_InvenioIII.txt" to the "FactoryCameraCfg_UsrDef.txt" and edit the parameters needed.
© 2022 DeltaPix Support

242 DeltaPix InSight - Manual and Help
22.11 Artray ARTCAM-130XQE-WOM Camera
ARTCAM-130XQE-WOM is a NIR camera which adopts a 1280 x 1024. The cameras have a high sensitive spectral response from 400 nm to 1200 nm. Attach the camera. Install the camera driver from the CD Browse to the driver location on the CD (also search sub directories).
Note that the camera does not (as you would expect) appear under 'Imaging Devices', but under 'Universal Serial Bus controllers.
© 2022 DeltaPix Support

Appendix 243
22.12 Artray ARTCAM-990 SWIRTEC camera
ARTCAM-990 SWIRTEC is a camera which adopts a 1280 x 1024. The cameras have a high sensitive spectral response from 400 nm to 1700 nm.
22.12.1 Artray ARTCAM-990 SWIRTEC - Driver installation
Attach the camera. Install the camera driver from the CD Browse to the driver location on the CD (also search sub directories).
Select the Windows10_64 bit driver.
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246 DeltaPix InSight - Manual and Help
The camera will appear as a Universal Serial Bus controlle.
Details about the installed driver.
22.12.2 Artray ARTCAM-990 SWIRTEC - Basic setup
Example of running the ARTCAM 990SWIRTEC
© 2022 DeltaPix Support

248 DeltaPix InSight - Manual and Help
To make the image appear right, select the Flipping = None. © 2022 DeltaPix Support

Appendix 249
In the Settings, the camera cooling can be enabled by setting a TEC PWM value greater then zero. The maximum PWM value is 80. A value of 20-30 will effectively cool the camera.
22.13 Allied Vision - GOLDEYE
A set of Alllied Vision cameras are integrated: GOLDEYE G-032 Goldeye-033 SWIR TEC1 Mako G-040B Goldeye G-030
These cameras are for special purposes, as example: The Goldeye G-032 TEC1 is a shortwave infrared camera for the spectral range from 900nm to 1700nm. High frame rates of 100 fps at full resolution (636 x 508), strong sensor cooling, as well as a high intra-scene dynamic range of more than 73 dB enable to apply the camera in various application areas and under varying operating conditions. Note: These cameras requires a special DeltaPix InSight dongle bit.
© 2022 DeltaPix Support

250 DeltaPix InSight - Manual and Help Contrast setting For this camera the contrast can be set using an interactive histogram tool: The tool shows in the tabbed control, along with the "Panorama View".
The live preview histogrm is show (the red curve) along with a line - defined by (mouse movable endpoints). The line defines the linear LUT transfer function.

The default 1:1 view.

Appendix 251
Inverted 1:1 view
Contrast enhanced view wit optional pseudo coloring. Contrast adjustment by dragging line. Parameter setting
© 2022 DeltaPix Support

252 DeltaPix InSight - Manual and Help The GOLDEYE only has gain 1 or 32, Auto-contrast OFF or ON.
© 2022 DeltaPix Support

22.14 Conneting H4KWSD08DPX by WiFi

Appendix 253

Make sure that you have at least v2.8 Attach network adapter to the USB3 port.

254 DeltaPix InSight - Manual and Help
Depending on your network select 2.4G or 5G (recommended 2.4G)
22.15 TEKLEAD Scanner
Bar code scanners can be used to identify fixed objectives or click stops. The 1D or 2D code should contain the number of the objective, objectives ordered from 1 - N, where 1 selects the 1st objective. Example: The code should contain the "<3>" string to select objective #3, Note: Objectives must be labeled from 1, and in the same order as defined in the optics editor.
Barcodes can be generated on-line, e.g. by https://barcode.tec-it.com/en/QRCode Sample 2D codes:
© 2022 DeltaPix Support

Appendix 255

256 DeltaPix InSight - Manual and Help
22.15.1 TEK_E1_CCD
To set the TEK_E1_CCD into VCOM mode, scan the code here:
22.15.2 TEK_E2_USB
To set the TEK_E2_USB into VCOM mode, scan the code here:
© 2022 DeltaPix Support

Index
- E -
ErgoControl 150

Index

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