3M Attest Mini Auto-reader 490M System Quick Reference Guide

*Refero t 3M ™ Attest Mini Auto-reader 490M Operator Manual and 3M™ Attest™ Biological Indicator 1295, 1491 and 1492V Instructions for Use for complete instructions and other important information.

3M™ Attest™ Mini Auto-reader 490M System* Quick ...

Quick Reference Guide. NOTES: • Activate and incubate 1295 BI within. 1 hour of completion of the VH2O2 sterilization cycle. • Gloves are recommended when.

Refer to 3M™ Attest™ Mini Auto-reader 490M Operator Manual and 3M™ Attest™ Biological Indicator 1295, 1491 and 1492V Instructions for Use for complete.

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Attest Quick Reference Poster 490M Dual 11x17 Rev0920 LR
3MTM AttestTM Mini Auto-reader 490M System* Quick Reference Guide
VH2O2 BI Preparation and Use

1 Inspect Remove BI from pouch and reseal. Verify media ampoule is intact and indicator stripes on cap are blue.

2 Pouch
Place BI and chemical indicator (CI) in peel
pouch indicated for use in vaporized hydrogen
peroxide (VH2O2) sterilization processes.

3 Process
Seal pouch and place in sterilizer with
white side facing up. Process load as
recommended by sterilizer manufacturer.

STEAM and VH2O2 BI Activation and Incubation

4 Identify
After completion of cycle, inspect and
verify media ampoule is intact and indicator
stripes on cap have turned towards pink.
Identify the BI by writing on the label.

1 Crush Squeeze BI in activator to close cap and crush media ampoule.

2 Flick Remove BI and flick wrist to ensure media flows to growth chamber.

3MTM AttestTM Mini Auto-reader 490M
Positive Control BI For both Steam and VH2O2, each day a processed BI is incubated, activate and incubate an unprocessed BI as a positive control. The control BI should be from the same lot code as the processed BI.
Results After Incubation Control BI: Positive (+) Acceptable Processed BI: Negative (-) Acceptable Sterilization Processed BI: Positive (+) Failed Sterilization
Remaining Incubation Minutes

3 Inspect Verify media has transferred to BI growth chamber at the vial bottom.

4 Incubate Place activated BI in any well. Display shows remaining minutes of incubation or test results. DO NOT REMOVE BI until the (+) or (-) symbol indicates the test is complete.
NOTES:  · Activate and incubate 1295 BI within
1 hour of completion of the VH2O2 sterilization cycle. · Gloves are recommended when activating 1295 BI. · Allow 1491 and 1492V BIs to cool 10 minutes prior to activation.
Touch Button (alarm off)

Interpretation of 3MTM Sterilization Assurance Core Products

STEAM Indicator 3MTM AttestTM BI 1491 and 1492V
3MTM ComplyTM Indicator Tape 1322

Unexposed

Acceptable Exposed

1491

1492V

1491

1492V

After processing, indicator on cap turns light brown or darker.

VH2O2 Indicator 3MTM AttestTM BI 1295
3MTM ComplyTM Indicator Tape 1228

3MTM ComplyTM Indicator Tape 1355

After processing, indicator stripes turn dark-brown/black. After processing, indicator stripes turn dark-brown/black.

3MTM AttestTM Tri-Metric Chemical Indicator 1348/1348E

Unexposed

Acceptable Exposed Color Range

After processing, indicator stripes turn toward pink. After processing, indicator stripes turn toward pink. After processing, a color change towards pink should have entered the ACCEPT window.

STEAM Indicator 3MTM AttestTM Steam Chemical Integrator 1243
3MTM ComplyTM Bowie-Dick Plus Test Pack 00135LF 3MTM ComplyTM Early Warning Test Sheet (above) 3MTM ComplyTM Bowie-Dick Test Sheet (below)

Unprocessed

Acceptable Processed PASS After processing, indicator bar crosses into the ACCEPT region.

Unacceptable Processed FAIL If the color bar is in the REJECT region or on the line, it is considered a fail.

PASS

FAIL

FAIL

FAIL

When indicator ink circle on Early Warning Test sheet is lighter than the color standard, this may indicate that sterilizer maintenance should be scheduled.

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PASS

PASS

After processing, a uniform color change to dark brown/black is a pass.

FAIL

FAIL

Fail result is indicated by a non-uniform color development, with a lighter colored area, usually near the center.

© 2020 3M. All rights reserved. 3M, Attest and Comply are marks and/or registered marks of 3M. Unauthorized use prohibited. 70-2011-7924-2 Rev 9/20

*Refer to 3MTM AttestTM Mini Auto-reader 490M Operator Manual and 3MTM AttestTM Biological Indicator 1295, 1491 and 1492V Instructions for Use for complete instructions and other important information. Refer to the indications for use provided in the biological indicator Instructions for Use. The indications for use should align with the sterilization cycle to be monitored. Refer to the Instructions for Use for complete instructions and other important information related to the use of all other 3M products included in this Guide.

Health Care Help Line 1-800-228-3957

Learn more about 3MTM Sterilization Assurance products at go.3M.com/sterilization


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