Instruction manual

8 avr. 2023 — vwr.com I Instruction manual VWR Micro Star 21. 25 / 87. Technical Specifications. 1. 6. 5. PCR 8 x 8 Rotor. Items Supplied. Item. VWR Cat No . Quantity.

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Manual VWR

Instruction manual
VWR Micro Star 21 and Micro Star 21R Centrifuge Series
NA cat. no: Micro Star 21: 76533-876 Micro Star 21R: 76533-878
Version 01 Issue 03/2022

Legal address of Manufacturer
Avantor One Radnor Corporate Center Building One, Suite 200 100 Matsonford Road Radnor, PA 19087 1 610 386 1700
Country of Origin
Germany

Contents
Preface 6 Intended Use 6 Signal Words and Symbols 6
Symbols used on Centrifuge and Accessories6 Symbols used in the Instruction Manual7 Safety Instructions 7
1. Technical Specifications12 1. 1. List of Centrifuges 12 1. 2. List of Rotors 12 1. 3. Technical Data 13
VWR Micro Star 2113 VWR Micro Star 21R 14 1. 3. 1. Directives and Standards15 1. 4. Mains Supply 16 1. 5. Refrigerants 16 1. 6. Rotor Specifications 17 1. 6. 1. 24 x 1.5/2.0 mL Rotor17 1. 6. 2. Dual Row 18 x 2.0/0.5 mL Rotor 19 1. 6. 3. 36 x 0.5 mL Rotor 21 1. 6. 4. 10 x 5 mL Rotor 23 1. 6. 5. PCR 8 x 8 Rotor 25 1. 6. 6. PCR 4 x 8 Rotor 27
2. Transport and Set Up29 2. 1. Unpacking 29
Items Supplied29 2. 2. Location 30 2. 3. Transporting 32 2. 4. Mains Connection 33

3. Operation34 3. 1. Control Panel 34 3. 2. Power on / off 35
3. 2. 1. To power on the centrifuge35 3. 2. 2. To power off the centrifuge 35 3. 2. 3. Audible Alarm35 3. 3. Open / Close the Centrifuge Lid 36 3. 4. How to Operate a Rotor 37 3. 4. 1. How to install a rotor 37 3. 4. 2. How to remove a rotor38 3. 4. 3. Rotor Lid39 3. 4. 4. Load the Rotor41
RCF Value Explained43 3. 5. Entering Centrifugation Parameters 44
3. 5. 1. Selecting Speed or RCF-Value44 3. 5. 2. Pre-selecting Speed44 3. 5. 3. Pre-selecting the RCF-Value 45 3. 5. 4. Pre-selecting Run Time 45 3. 5. 5. Pre-selecting Temperature46 3. 5. 6. Pre-Tempering the Centrifugation Chamber47 3. 5. 7. Changing the Settings During the Run47 3. 6. Centrifugation 48
Starting Centrifugation48 Stopping Centrifugation48 3. 7. Short-term Centrifugation 49 3. 8. Aerosol-tight Applications 49 Basic Principles 49 Replacing Seals50 Fill Level50 Checking Aerosol Tightness51 Quick Test51
4. Maintenance and Care52 4. 1. Cleaning Intervals 52

4. 2. Basics 52 4. 3. Cleaning 53
Cleaning the Filter Unit54 4. 4. Disinfection 55 4. 5. Decontamination 55 4. 6. Autoclaving 56 4. 7. Service 56 4. 8. Lifetime 56 4. 9. Shipping 57 4. 10. Storage 57
5. Troubleshooting58 5. 1. Mechanical Emergency Lid Release 58 5. 2. Ice Formation 59 5. 3. Troubleshooting by Guide 59
5. 3. 8. Information for the Customer Service 62 Technical service 63
Web Resources 63 Warranty 63 Compliance with local laws and regulations 63 Equipment disposal 64
6. Chemical Compatibility 65

Preface

Intended Use
This centrifuge is a laboratory product designed for the separation of aqueous solutions and suspensions of different densities or particle sizes by generation of Relative Centrifugal Force. It separates specimens either alone or after addition of reagents or other additives. This centrifuge and its accessories should be operated by trained specialists only.

Signal Words and Symbols

Signal Word WARNING CAUTION NOTICE

Degree of Hazard
Indicates a hazardous situation that, if not avoided, could result in death or serious injury.
Indicates a hazardous situation that, if not avoided, could result in minor or moderate injury.
Indicates information considered important, but not hazard-related.

Symbols used on Centrifuge and Accessories
Observe the information contained in this instruction manual to keep yourself and your environment safe.

General hazard

Danger of cuts

Biological hazard

Hazard caused by hot surface.

Refer to instruction manual

Disconnect mains plug

Batch code

Manufacturer

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Symbols used in the Instruction Manual
Observe the information contained in the instruction manual to keep yourself and your environment safe.

General hazard

Electrical hazard

Biological hazard
Hazard caused by flammable materials
Hazard caused by hot surface.

Danger of cuts
Risk of crushing
Indicates information considered important, but not hazard-related.

Safety Instructions

WARNING

Not following these safety instructions can lead to hazardous situations that, if not avoided, could result in death or serious injury. Observe the safety instructions. The centrifuge is to be used for its intended use only. Improper use can cause damages, contamination, and injuries with fatal consequences. The centrifuge must be operated by trained personnel only. It is the obligation of the operator to make sure that the proper protective clothing is used. Mind the "Laboratory Biosafety Manual" of the World Health Organization (WHO) and the regulations in your country. Keep a safety zone of minimum 30 cm around the centrifuge. Refer to "Figure 1: Safety Zone" on page 31 Persons and hazardous substances must be kept out of this safety zone while centrifuging. Do not modify the centrifuge and its accessories in any unauthorized way. Do not operate a centrifuge if its housing is open or incomplete.

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WARNING

Risk of damage due to incorrect power supply. Make sure that the centrifuge is plugged only into a power outlet that has been properly grounded. Do not use a power supply cable with inadequate rating.

WARNING

Risk from handling hazardous substances. When working with corrosive samples (salt solutions, acids, bases), the accessories and the centrifuge have to be cleaned thoroughly. Extreme care should be taken with highly corrosive substances that can cause damage and impair the mechanical stability of the rotor. These should only be centrifuged in fully sealed tubes. The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone environment. Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety precautions. When centrifuging any hazardous materials mind the "Laboratory Biosafety Manual" of the World Health Organization (WHO) and any local regulations. When centrifuging microbiological samples from the Risk Group II (according to the "Laboratory Biosafety Manual" of the World Health Organization (WHO)), aerosol-tight biological seals have to be used. Visit the Internet page of the World Health Organization (www.who.int) for the "Laboratory Biosafety Manual". For materials in a higher risk group, extra safety measures must be taken. If toxins or pathogenic substances have contaminated the centrifuge or its parts, appropriate disinfection measures have to be taken ("Disinfection" on page 55). If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area immediately. Make sure to use the proper accessories for your applications to avoid hazardous contamination. In any case of severe mechanical failure, such as rotor or bottle crash, personnel should be aware that the centrifuge is not aerosol-tight. Leave the room immediately. Contact the customer service. Aerosols need time to settle before opening the centrifuge after a crash. Ventilated centrifuges bear a higher risk of being contaminated after a crash than refrigerated centrifuges.

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WARNING

Risk of contamination. Potential contaminations will not remain in the centrifuge while the device is operated. Take appropriate protection measures to prevent spread of contaminations. A centrifuge is no closed containment.

WARNING

Damage to health from centrifuging explosive or flammable materials or substances. Do not centrifuge explosive or flammable materials or substances.

WARNING

Serious injuries can occur if you touch a spinning rotor with your hands or tools. A rotor can still be spinning after a power failure occurs. Do not open the centrifuge before the rotor has stopped spinning. Do not touch a spinning rotor. Open the centrifuge only if the rotor has stopped spinning. Never use your hands or tools to stop a spinning rotor. The emergency lid release may be used in emergencies only to recover the samples from the centrifuge, for example, during a power failure ("Mechanical Emergency Lid Release" on page 58).

WARNING

Risk of injuries from defective lid spring Make sure that the centrifuge lid can be opened completely and that it will stay in its position. Regularly check the lid springs for their proper function. Do not operate the centrifuge with a defective lid spring. Let an authorized service technician replace defective lid springs.

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CAUTION

Safety can be impaired by wrong loading and worn accessories. Always make sure that the load is as equally distributed as possible. Do not use rotors and accessories which show any signs of corrosion or cracks. Contact customer service for further information. Do not operate the centrifuge with an unbalanced rotor. Use only rotors which have been loaded properly. Never overload the rotor. Make sure that rotors and accessories are installed properly before operating the centrifuge. Follow the instructions in section "3. 4. How to Operate a Rotor" on page 37.

CAUTION

Physical harm caused by ignoring operative basics. Operate the centrifuge with a properly installed rotor. Do not move the centrifuge while it is running. Do not lean on the centrifuge. Do not put anything on the centrifuge while it is running. The centrifuge housing is not to be opened by the operator.

CAUTION

Due to air friction sample integrity may be affected. The temperature of the rotor may rise significantly while the centrifuge is spinning. Ventilated units lead to a heat up of the rotor above the ambient temperature. Refrigerated units can have a deviation from displayed and set temperature to the sample temperature. Make sure the centrifuge temperature control capabilities meet your application specification. If necessary make a test run.

NOTICE

Protection capability may be impaired due to using unapproved accessories. Use only accessories for this centrifuge which have been approved by VWR International. For a list of approved accessories refer to "List of Rotors" on page 12. Exceptions to this rule are commercially available glass or plastic centrifuge labware, provided they have been designed to fit in the rotor or the adapter cavities and are approved for the speed or the RCF value of the rotor.

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NOTICE

To shut down the centrifuge: Press the "Stop" key. Turn off the centrifuge at the main switch. Pull out the power supply plug. In an emergency disconnect the power supply. Make sure that the main switch and power supply plug can be accessed easily when setting up the centrifuge. The grounded electrical socket should be well accessible and located outside of the safety zone.

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Technical Specifications

1. Technical Specifications

1. 1. List of Centrifuges

VWR Cat. No. 76533-876 76533-878

Centrifuge Micro Star 21, 120 V ±10 %, 60 Hz Micro Star 21R, 120 V ±10 %, 60 Hz

Table 1: List of Centrifuges

1. 2. List of Rotors

VWR Cat. No. 97000-052 76081-580 76081-582 76081-068 76081-586 76081-584

Description 24 x 1.5/2.0 mL rotor with ClickSeal biocontainment lid Dual Row 18 x 2.0/0.5 mL rotor with screw-on lid 36 x 0.5 mL rotor with screw-on lid 10 x 5 mL rotor with ClickSeal biocontainment lid PCR 8 x 8 rotor with screw-on lid PCR 4 x 8 rotor with ClickSeal biocontainment lid

Table 2: Rotors

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Technical Specifications

1. 3. Technical Data

VWR Micro Star 21
Speed Range (depending on the rotor) RCF Value at max. Speed Running Time Noise Level at max. Speed Maximum Kinetic Energy Average Heat Dissipation Environmental Conditions For Storage and Shipping For Operation
Pollution Degree Overvoltage Category IP Dimensions

30014800 rpm 21100 x g unlimited < 50 dB (A) (1 m in front of the unit at 1.6 m height) 2.37 kNm 0.2 kW/h
Temperature: 2 °C to 50 °C Use in interior spaces Altitudes of up to 3000 m above sea level Temperature: 2 °C to 40 °C Max. relative humidity 80% up to 31 °C; decreasing linearly to 50% relative humidity at 40 °C 2 II 20

Height Width Depth
Weight (with rotor)

23.5 cm (9.2 in) 23.0 cm (9.0 in) 36.5 cm (14.4 in)
11 kg (24,2 lbs)

Table 3: Technical Data VWR Micro Star 21

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Technical Specifications

VWR Micro Star 21R
Speed Range (depending on the rotor) RCF Value at max. Speed Running Time Noise Level at max. Speed Maximum Kinetic Energy Average Heat Dissipation Environmental Conditions For Storage and Shipping For Operation
Pollution Degree Overvoltage Category IP Dimensions Height Width Depth Weight (with rotor)

30014800 rpm 21100 x g unlimited < 50 dB (A) (1 m in front of the unit at 1.6 m height) 2.37 kNm 0.3 kW/h
Temperature: 2 °C to 50 °C Use in interior spaces Altitudes of up to 3000 m above sea level Temperature: 2 °C to 40 °C Max. relative humidity 80% up to 31 °C; decreasing linearly to 50% relative humidity at 40 °C 2 II 20
28.5 cm (11.2 in) 33.0 cm (13.0 in) 45.0 cm (17.7 in) 28 kg (61.7 lbs)

Table 4: Technical Data VWR Micro Star 21R

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Technical Specifications

1. 3. 1. Directives and Standards

Region North America

Directive

Table 5: Directives and Standards

Standards ANSI/UL 61010-1 3.1 Edition UL 61010-2-020 3rd Edition UL 61010-2-011 2nd Edition UL 61010-2-101 3rd Edition FCC Part 15 ICES-001 EN ISO 14971 ISO 9001

NOTICE: This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. However, there is no guarantee that interference will not occur in a particular installation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures:
» Reorient or relocate the receiving antenna. » Increase the separation between the equipment and receiver. » Connect the equipment into an outlet on a circuit different from that to which the
receiver is connected. » Consult the dealer or an experienced radio/TV technician for help.

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Technical Specifications

1. 4. Mains Supply

VWR Cat. Centrifuge Voltage No.

76533876

VWR Micro 120
Star 21

76533878

VWR Micro 120
Star 21R

Frequ- Rated Power

Equipment

ency Current Consumption Fuse

3.4

220

6.3 AT

4.4

380

8A 2-pole circuit breaker

Building Fuse 16 A
15 A

Table 6: Mains Supply

1. 5. Refrigerants

VWR Cat. Centrifuge Refrigerant Quantity No.

Low and high side max. pressure

GWP

76533878

VWR Micro R-134a
Star 21R

0.26 kg

21 bar

1430

Contains fluorinated greenhouse gases in a hermetically sealed system.

Table 7: Refrigerants

CO2e 0.37 t

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Technical Specifications

1. 6. Rotor Specifications

1. 6. 1. 24 x 1.5/2.0 mL Rotor

Supplied with VWR Micro Star 21/21R

Item 24 x 1.5/2.0 mL Rotor O-Ring Grease

Quantity 1 1

Table 8: Items Supplied

General Technical Data

Maximum Permissible Load Maximum Number of Cycles Radius (max. / min.) Angle Aerosol-tight Max. Autoclaving Temperature

24 x 4 g 50000 8.6 cm / 5.1 cm 45° Yes 121 °C

Table 9: General Technical Data 24 x 1.5/2.0 mL Rotor

Rotor Performance Data

Micro Star 21 24 x 1.5/2.0 mL Rotor Voltage Maximum Speed Maximum RCF-Value Acceleration / Braking Time Sample Heating at max speed, 23 °C ambient temperature, 60 min running time

120 V 14800 21100 13 s / 13 s
36 °C

Micro Star 21R 24 x 1.5/2.0 mL Rotor

Voltage

120 V

Maximum Speed

14800

Maximum RCF-Value

21100

Acceleration / Braking Time

12 s / 13 s

Min. Temperature at max. Speed, Ambient 0 °C
Temperature of 23 °C

Table 10: 24 x 1.5/2.0 mL Rotor Performance Data

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Technical Specifications

Accessories

Description
0.5/0.6 mL microliter tube adapters, pack of 24 0.25/0.4 mL microliter tube adapters, pack of 24 0.2 mL PCR tube adapters, pack of 24 ClickSeal biocontainment lid Replacement O-ring set for ClickSeal lid 521-1103

VWR Cat. Rotor

No.

Capacity

20301-384 24 x 0.5/0.6

20301-382 24 x 0.25/0.4

20301-380 24 x 0.2

97000-056 -

89423-845 -

Max. Tube Dimensions 7 x 32 6 x 24 6 x 24 -
-

Table 11: Accessories 24 x 1.5/2.0 mL Rotor

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Technical Specifications

1. 6. 2. Dual Row 18 x 2.0/0.5 mL Rotor

Items Supplied

Item

VWR Cat. No.

Dual Row 18 x 2.0/0.5 mL Rotor 76081-580

Quantity 1

Table 12: Items Supplied Dual Row 18 x 2.0/0.5 mL Rotor

General Technical Data

Maximum Permissible Load Maximum Number of Cycles Radius (max. / min.) Angle Aerosol-tight Max. Autoclaving Temperature

8 x 4 g + 8 x 0.5 g 50000 8.5 cm / 4.8 cm 45° No 121 °C

Table 13: General Technical Data Dual Row 18 x 2.0/0.5 mL Rotor

Rotor Performance Data

Micro Star 21 Dual Row 18 x 2.0/0.5 mL Rotor

Voltage

120 V

Maximum Speed

14800

Maximum RCF-Value

20800

Acceleration / Braking Time

12 s / 13 s

Sample Heating at max speed, 23 °C 36 °C
ambient temperature, 60 min running time

Micro Star 21R Dual Row 18 x 2.0/0.5 mL Rotor

Voltage

120 V

Maximum Speed

14800

Maximum RCF-Value

20800

Acceleration / Braking Time

11 s / 13 s

Min. Temperature at max. Speed, Ambient 4 °C
Temperature of 23 °C

Table 14: Dual Row 18 x 2.0/0.5 mL Rotor Performance Data

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Technical Specifications

Accessories

Description Screw-on lid

VWR Cat. Rotor

No.

Capacity

76537-066 -

Table 15: Accessories Dual Row 18 x 2.0/0.5 mL Rotor

Max. Tube Dimensions
-

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Technical Specifications

1. 6. 3. 36 x 0.5 mL Rotor

Items Supplied

Item 36 x 0.5 mL Rotor

VWR Cat. No. 76081-582

Table 16: Items Supplied 36 x 0.5 mL Rotor

Quantity 1

General Technical Data

Maximum Permissible Load Maximum Number of Cycles Radius (max. / min.) Angle Aerosol-tight Max. Autoclaving Temperature

36 x 0.5 g 50000 7.9 cm / 5.0 cm 45° No 121 °C

Table 17: General Technical Data 36 x 0.5 mL Rotor

Rotor Performance Data

Micro Star 21 36 x 0.5 mL Rotor Voltage Maximum Speed Maximum RCF-Value Acceleration / Braking Time Sample Heating at max speed, 23 °C ambient temperature, 60 min running time

120 V 14800 19300 10 s / 11 s
34 °C

Micro Star 21R 36 x 0.5 mL Rotor

Voltage

120 V

Maximum Speed

14800

Maximum RCF-Value

19300

Acceleration / Braking Time

9 s / 11 s

Min. Temperature at max. Speed, Ambient 0 °C
Temperature of 23 °C

Table 18: 36 x 0.5 mL Rotor Performance Data

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Technical Specifications

Accessories
Description
Screw-on lid Table 19: Accessories 36 x 0.5 mL Rotor

VWR Cat. Rotor

No.

Capacity

76537-066 -

Max. Tube Dimensions
-

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Technical Specifications

1. 6. 4. 10 x 5 mL Rotor

Items Supplied

Item 10 x 5 mL Rotor O-Ring Grease O-Ring Set

VWR Cat. No. 76537-068 76537-070 89423-845

Table 20: Items Supplied 10 x 5 mL Rotor

Quantity 1 1 1

General Technical Data

Maximum Permissible Load Maximum Number of Cycles Radius (max. / min.) Angle Aerosol-tight Max. Autoclaving Temperature

10 x 9 g 50000 8.3 cm / 4.2 cm 41° Yes 121 °C

Table 21: General Technical Data 10 x 5 mL Rotor

Rotor Performance Data

Micro Star 21 10 x 5 mL Rotor Voltage Maximum Speed Maximum RCF-Value Acceleration / Braking Time Sample Heating at max speed, 23 °C ambient temperature, 60 min running time

120 V 14800 20326 13 s / 13 s
36 °C

Micro Star 21R 10 x 5 mL Rotor

Voltage

120 V

Maximum Speed

14800

Maximum RCF-Value

20326

Acceleration / Braking Time

12 s / 13 s

Min. Temperature at max. Speed, Ambient 0 °C
Temperature of 23 °C

Table 22: 10 x 5 mL Rotor Performance Data

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Technical Specifications

Accessories
Description
ClickSeal biocontainment lid Replacement O-ring set for ClickSeal lid 521-1103 Table 23: Accessories 10 x 5 mL Rotor

VWR Cat. Rotor

No.

Capacity

97000-056 -

89423-845 -

Max. Tube Dimensions -
-

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Technical Specifications

1. 6. 5. PCR 8 x 8 Rotor

Items Supplied

Item PCR 8 x 8 Rotor

VWR Cat. No. 76081-586

Table 24: Items Supplied PCR 8 x8 Rotor

Quantity 1

General Technical Data

Maximum Permissible Load Maximum Number of Cycles Radius (max. / min.) Angle Aerosol-tight Max. Autoclaving Temperature

8 x 4 g (64 x 0.5 g) 50000 7.0 cm / 4.4 cm 60° No 121 °C

Table 25: General Technical Data PCR 8 x 8 Rotor

Rotor Performance Data

Micro Star 21 PCR 8 x 8 Rotor Voltage Maximum Speed Maximum RCF-Value Acceleration / Braking Time Sample Heating at max speed, 23 °C ambient temperature, 60 min running time

120 V 14800 17100 8 s / 9 s
32 °C

Micro Star 21R PCR 8 x 8 Rotor

Voltage

120 V

Maximum Speed

14800

Maximum RCF-Value

17100

Acceleration / Braking Time

7 s / 9 s

Min. Temperature at max. Speed, Ambient 0 °C
Temperature of 23 °C

Table 26: PCR 8 x 8 Rotor Performance Data

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Technical Specifications

Accessories
Description
Screw-on lid Table 27: Accessories PCR 8 x 8 Rotor

VWR Cat. Rotor

No.

Capacity

76537-066 -

Max. Tube Dimensions
-

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Technical Specifications

1. 6. 6. PCR 4 x 8 Rotor

Items Supplied

Item PCR 4 x 8 Rotor

VWR Cat. No. 76081-584

Table 28: Items Supplied PCR 4 x 8 Rotor

Quantity 1

General Technical Data

Maximum Permissible Load Maximum Number of Cycles Radius (max. / min.) Angle Aerosol-tight Max. Autoclaving Temperature

4 x 4 g (32 x 0.2 g) 50000 6.6 cm / 4.7 cm 45° Yes 121 °C

Table 29: General Technical Data PCR 4 x 8 Rotor

Rotor Performance Data

Micro Star 21 PCR 4 x 8 Rotor Voltage Maximum Speed Maximum RCF-Value Acceleration / Braking Time Sample Heating at max speed, 23 °C ambient temperature, 60 min running time

120 V 14800 16200 12 s / 13 s
33 °C

Micro Star 21R PCR 4 x 8 Rotor

Voltage

120 V

Maximum Speed

14800

Maximum RCF-Value

16200

Acceleration / Braking Time

11 s / 13 s

Min. Temperature at max. Speed, Ambient 0 °C
Temperature of 23 °C

Table 30: PCR 4 x 8 Rotor Performance Data

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Technical Specifications

Accessories
Description
ClickSeal biocontainment lid Replacement O-ring set for ClickSeal lid 521-1103 Table 31: Accessories PCR 4 x 8 Rotor

VWR Cat. Rotor

No.

Capacity

97000-056 -

89423-845 -

Max. Tube Dimensions -
-

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Transport and Set Up
2. Transport and Set Up
The shipping carton should be inspected upon delivery. When received, carefully examine for any shipping damage before unpacking. If damage is discovered, the delivering carrier should specify and sign for the damage on your copy of the delivery receipt. Open the carton carefully making certain that all parts ("Items Supplied" on page 29) are accounted for before packaging materials are discarded. After unpacking, if damage is found, report it to the carrier and request a damage inspection. Important: Failure to request an inspection of damage within a few days after receipt of shipment absolves the carrier from any liability for damage. You must call for a damage inspection.

NOTICE

It is your responsibility to make sure that the centrifuge is set up properly.

2. 1. Unpacking
Use the packing list when unpacking to verify that the complete unit has been received. Do not discard packing materials until all is accounted for.

Items Supplied

Item Centrifuge Power Supply Cable Rotor Allen Wrench Manuals on USB

Quantity 1 2 1 1 1

If any items are missing, contact VWR International.

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Transport and Set Up
2. 2. Location
Operate the centrifuge only indoors. The set up location must meet these requirements:
Keep a safety zone of minimum 30 cm around the centrifuge. Refer to "Safety Zone" on page 31. Persons and hazardous substances must be kept out of this safety zone while centrifuging. Centrifuges cause vibrations. Do not store sensitive devices or dangerous objects or substances in the safety zone. WARNING Risk of impact. The centrifuge can crush objects and persons in a radius of 30 cm while spinning. Keep a safety zone of 30 cm around the centrifuge for safe operation. Make sure that no one is in the safety zone while the centrifuge is spinning.
The supporting structure must meet these requirements: » Be stable, solid, rigid and free of resonance. » Be free from grease and dust. » Be applicable for horizontal set up of the centrifuge. It is not allowed to put anything under the centrifuge to compensate for an uneven surface. Do not operate the centrifuge on carts or stand-alone shelving that could move during operation or is inadequately sized for the centrifuge. » Be able to hold the weight of the centrifuge.
The centrifuge is not equipped with any means for leveling. The supporting structure must be suitably level to allow proper set up. CAUTION If you do not level the centrifuge, the centrifuge can crash because of imbalance. If you move the centrifuge, you must level it again. Do not move the centrifuge with a rotor attached to the drive shaft because damage can occur to the drive. Do not put anything below the centrifuge feet to level the centrifuge.
Do not expose the centrifuge, accessories and samples to heat and strong sunlight. CAUTION UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.
The set up location must be well ventilated at all times. The main switch and power supply plug must be easily accessible at all times. The
grounded electrical socket should be well accessible and located outside of the safety zone.
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Transport and Set Up
Figure 1: Safety Zone
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Transport and Set Up
2. 3. Transporting
Before transporting a centrifuge make sure that the power supply cable is unplugged and removed from the centrifuge. the rotor is removed.
CAUTION Damage to centrifuge or drive shaft due to movement of an installed rotor. Always remove the rotor before you transport the centrifuge. the centrifuge door is closed. CAUTION Hands can be squeezed by an open centrifuge door. Always close the centrifuge door before you transport the centrifuge. Before transporting a rotor make sure that all components, like adapters and buckets are removed to prevent dropping damage. the centrifuge is lifted at both sides and not at the front or back. WARNING Always lift the centrifuge on both sides. Never lift the centrifuge by its front or back.
Figure 2: Lifting the Centrifuge at Both Sides
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Transport and Set Up
2. 4. Mains Connection

Power Supply Switch; Mains Connection Figure 3: Mains Connection: Micro Star 21 (left); Micro Star 21R (right)
1. Turn off the power supply switch. 2. Make sure that the power supply cable specification agrees with the safety standards
of your country. 3. Make sure that the voltage and frequency are the same as the figures on the nameplate 4. Make sure that the power supply cable is plugged in properly.

NOTICE

Connect the centrifuge into grounded electrical sockets only.

NOTICE

Rated current is doubled during acceleration. Mind this load on the power supply line.

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3. Operation
3. 1. Control Panel

Operation

No. Item

Description

Speed / RCF The speed (rpm) or RCF value (x g) is displayed here. You can modify the value

Value

using the up and down MINUS / PLUS buttons . below. You can switch

between rpm and x g using the TOGGLE button .

Display Mode

Use the TOGGLE button to change the display mode. (speed / RCF value,

sample / chamber temperature, run time counter from start or preset speed on).

Running Time The running time is displayed here. You can modify the value using the MINUS /

PLUS buttons .

Temperature

The temperature is displayed here. You can modify the value using the up and

(SNOWFLAKE) down MINUS / PLUS buttons . You can pre-temper the centrifugation

chamber and the empty rotor before the centrifugation run starts using

SNOWFLAKE button.

PULSE

OPEN

START

STOP

NOTICE This function is only available on refrigerated centrifuges. Press PULSE to immediately start the centrifugation run and accelerate up to the maximal permissible end speed (depending on the used rotor). Releasing PULSE initiates a stopping process according to the set acceleration and braking curve. Press OPEN to activate the automatic door release (possible only if device is powered on and if the rotor is fully stopped). Press START to start a centrifugation run or to accept the current settings. Press STOP to manually end the centrifugation run.

MINUS / PLUS Use the up and down MINUS / PLUS buttons . to set the parameter in the

field directly above.

TOGGLE

Use the TOGGLE button to change the display mode.

Figure 4: Control Panel Overview
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Operation
3. 2. Power on / off
3. 2. 1. To power on the centrifuge
Push on the mains switch of the centrifuge to "1" to power it on. The centrifuge shows the actual value in the display. Speed and Time show 0. The display shows the current temperature of the sample.
3. 2. 2. To power off the centrifuge
Set the mains switch of the centrifuge to "0" to power it off. NOTICE The centrifuge is equipped with a special switch for balancing potential voltage discrepancies in the power grid. After pressing the mains switch the display therefore may still flash up to 10 seconds.
3. 2. 3. Audible Alarm Error
Accompanying all error messages, a warning signal is given out. Press any key to silence the warning signal. End of Run By default there is an acoustic signal at the end of any centrifugation run. To switch off this signal proceed as follows: 1. Keep the TOGGLE button pressed when you turn on the centrifuge.
The display shows:
or
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Operation

2. Press the up and down MINUS / PLUS buttons middle.
The acoustic signal is turned on or off. 3. Press STOP to confirm the pre-selected value.

under the TIME display in the

3. 3. Open / Close the Centrifuge Lid
To open the centrifuge lid Press the Open button on the control panel. The display shows the following:

To close the centrifuge lid Close the centrifuge lid by pressing down on it lightly in the middle or on both sides. The locking mechanism engages to close the lid safely. The lid should audibly click into place. Double-check whether the locking mechanism has engaged properly.

WA R N I N G

Do not use the mechanical emergency door release as regular procedure to open the centrifuge. Use the mechanical emergency door release only if a malfunction or power failure occurs and only when you have made sure that the rotor has stopped spinning (refer to "Mechanical Emergency Lid Release" on page 58).

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Operation
3. 4. How to Operate a Rotor
The approved rotors are listed in the "List of Rotors" on page 12. Operate the centrifuge only with rotors and accessories from this list.
3. 4. 1. How to install a rotor
1. Press the Open button control panel to open the lid of the centrifuge. 2. Hold the rotor over the centrifuge spindle. The two bars in the labeling on the upper
side of the rotor (a) must be aligned with the retaining pin (b) of the centrifuge spindle. a.
b.
3. Let the rotor slide down slowly. 4. Insert the Allen wrench into the centrifuge spindle and tighten clockwise. Hold the rotor
with the other hand. 5. Make sure that the rotor is properly installed by lifting it slightly on the handle. If the rotor
can be pulled up, then it must be re-clamped to the drive shaft. 6. Make sure the rotor spins freely by turning it manually.
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Operation

WA R N I N G

If the rotor cannot be properly locked in place after several attempts, then the rotor fixation is defective and you are not permitted to operate the rotor. Check for any damage to the rotor: Damaged rotors must not be used. Keep the drive shaft area of the rotor clear of objects.

CAUTION Do not force the rotor onto the drive shaft. If the rotor is very light, it may be necessary to carefully press it onto the drive shaft with little force.

CAUTION Make sure that the rotor is properly locked on the drive shaft before each use by pulling at its handle.

CAUTION

Risk of burning on hot surfaces. When installing or removing a rotor you may accidentally touch the spindle or motor surface. The centrifuge spindle and the motor may be hot (>55 °C). Be aware of this risk and proceed carefully when you change a rotor after a run or wait until the motor has cooled down.

Before installing a rotor
Remove any dust, foreign objects or residue from the chamber, if necessary. Inspect the thread and O-ring of the motor spindle. Both parts must be clean and
undamaged. CAUTION Do not install the rotor when the temperature difference between the shaft and the rotor lock is >20 °C. Otherwise the rotor might jam.
3. 4. 2. How to remove a rotor
1. Press the Open button on the control panel to open the lid of the centrifuge. 2. If necessary, remove samples, adapters or buckets. 3. Unscrew the rotor fixation with the Allen wrench. 4. Grasp the rotor in the middle. Pull the rotor directly upwards and remove it from the
centrifuge spindle. Make sure not to tilt the rotor while doing this. CAUTION Be careful when you change a rotor after a run. The centrifuge spindle and motor may be hot (>55 °C) and burn your skin.

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Operation
Aerosol-tight Rotors When using an aerosol tight lid the rotor can be removed with the lid closed. This is to protect you and the samples.
NOTICE Make sure that all components are safely fixed before you carry a rotor.
3. 4. 3. Rotor Lid
CAUTION Unapproved or incorrectly combined rotors and accessories can cause serious damage to the centrifuge.
Rotors with ClickSealTM Biocontainment Lid
Open The rotor lid is retained by the integrated central rotor nut. Unlock and lift the lid by keeping the red unlocking button pressed at the handle
Close 1. Put the rotor lid on the rotor nut. 2. Push the rotor lid down until you see and hear the lock click. If the lid does not close at all or only by force, make sure that the sealing rings are correctly in position. Clean and lubricate them if necessary. Examine the lid mechanism for dirt and correct functionality. Replace damaged parts immediately. Rotors with Screw-on Lid
Open The rotor lid is screwed to the rotor body. 1. Turn the rotor handle counter-clockwise to remove the lid. 2. Lift the rotor lid.
Close The rotor lid is screwed to the rotor body. 1. Put the rotor lid onto the rotor. 2. Turn the rotor handle clockwise to install the lid. Operating the Rotor without the Rotor Lid If you plan to operate the rotor without the lid you must remove the seals.
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Operation
a = Large seal in outer groove of rotor body; b = Small seal in groove of rotor collar Figure 5: Rotor Lid Seals CAUTION Loose parts may damage the centrifuge. When operating the rotor without the lid the seals are not fixed in their position and can damage the centrifuge. Tube Caps Always close the tube caps. Open caps can tear loose during operation and cause damage.
= open cap; = closed cap Figure 6: Tube caps
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Operation
3. 4. 4. Load the Rotor Balance Loading
Load the compartments evenly. Balance opposite loads. Correct Loading
Incorrect Loading
Before Loading a Rotor Before loading a Rotor 1. Inspect the rotor and all accessory parts for damage, such as cracks, scratches, or
traces of corrosion. 2. Inspect the centrifugation chamber and drive shaft for damage, such as cracks,
scratches, or traces of corrosion. 3. Check the suitability of the rotor and other used accessories against the Chemical
Compatibility Chart. Refer to "Chemical Compatibility" on page 65. 4. Make sure that:
» tubes fit in the rotor. » the tubes do not touch the rotor lid.
CAUTION Incorrect loading can lead to damage. Always load the rotor symmetrically to avoid imbalance, noisy spinning and possible damage.
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Operation
CAUTION When using an aerosol-tight rotor lid, verify that the sample tubes don't interfere with the rotor lid and don't compromise its sealing efficiency.

CAUTION

Tubes may open and break during centrifugation because they do not fit properly to the cavities. Contamination may occur. Make sure that the length and width of the tubes are fitting into cavities. Do not use tubes that are too short or too thick for the cavities.

Maximum Loading Each rotor is designed to run with its maximum load at maximum speed. The safety system of the centrifuge requires that the rotor is not overloaded. The rotors are designed to work with substance mixtures with a density of up to 1.2 g/ml. If the admissible maximum load is exceeded, the following steps need to be taken:
Reduce the fill level.
Reduce the speed. Use the following formula or the table given for each rotor in the chapter "1. 6. Rotor Specifications" on page 17 to calculate the maximum admissible speed for a given load:

nadm = admissible maximum application speed nmax = maximum rated speed wmax = maximum rated load wapp = applied load

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Operation
RCF Value Explained
The relative centrifugal force (RCF) is given as a multiple of the force of gravity (g). It is a unitless numerical value which is used to compare the separation or sedimentation capacity of various centrifuges, since it is independent of the type of device. Only the centrifuging radius and the speed are used for calculation:
r = centrifuging radius in cm n = rotational speed in rpm The maximum RCF value is related to the maximum radius of the tube opening. Remember that this value is reduced depending on the tubes, buckets and adapters used. This can be accounted for in the calculation above if required.
Use of Tubes and Consumables Make sure that the tubes and bottles used in the centrifuge are: rated to or above the selected RCF to be spun at, never used not below their minimum fill volume and never above their maximum fill
volume, not used above their design life (age or number of runs), undamaged, fitting well into the cavities. Please refer to manufacturers' data sheets for further information.
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Operation
3. 5. Entering Centrifugation Parameters
NOTICE Due to limited display digits there is a need to round the values. The direct comparison between the two values speed and RCF is therefore restricted.
3. 5. 1. Selecting Speed or RCF-Value
Press the TOGGLE button to switch between the two modes. When the lower indicator is lit the display shows the speed.
When the upper indicator is lit the display shows the RCF value.
3. 5. 2. Pre-selecting Speed
1. Enter the desired value by pressing up and down MINUS / PLUS buttons repeatedly until the desired value shows. You can adjust the speed in 100 rpm increments.
2. Press START to confirm the pre-selected value. If you do not press any button, the display flashes for a few seconds. The new preselected value is now stored and the display shows the actual value.
NOTICE The centrifuge speed can be set to a minimum of 300 rpm. The maximum speed depends on the centrifuge variant. NOTICE Avoid if possible speed ranges close to natural resonances of the system. Runs at resonance speeds may cause vibration and have an adverse effect on the quality of separation.
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Operation
3. 5. 3. Pre-selecting the RCF-Value
1. Enter the desired value by pressing the up or down MINUS / PLUS buttons repeatedly, until the desired value shows. You can adjust the RCF pre-selected value in steps of 100 x g.
2. Press START to confirm the pre-selected value. If you do not press any button, the display flashes for a few seconds. The new preselected value is now stored and the display shows the actual value.
NOTICE The RCF value can be set to a minimum of 100 x g. The displayed RCF value is always corresponding to the maximum of centrifuge radius of the 24 x 1.5 / 2.0 mL Rotor. Refer to "RCF Value Explained" on page 43 for further information. NOTICE Avoid if possible speed ranges close to natural resonances of the system. Runs at resonance speeds may cause vibration and have an adverse effect on the quality of separation.
3. 5. 4. Pre-selecting Run Time
NOTICE You can select a run time between 1 and 99 min or continuous operation. 1. Enter the desired value by pressing the up or down MINUS / PLUS buttons below
the display in the middle repeatedly, until the desired value shows. You can adjust the run time in steps of 1 min. 2. Press START to confirm the pre-selected value. If you do not press any key, the display flashes for a few seconds. The new preselected value is now stored and the display shows the actual value.
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Operation

Continuous Operation 1. Press the up or down MINUS / PLUS buttons

until hd is shown.

2. During continuous operation, the centrifuge will continue running until you stop it manually with STOP.
CAUTION Please note that the lifetime of rotor tubes is limited, in particular if the tubes are made of plastics materials. Continuous operation (extended use) may cause damage to them.
3. 5. 5. Pre-selecting Temperature
You can determine the sample temperature in °C. Proceed as follows: 1. Enter the desired value by pressing the up or down MINUS / PLUS buttons under
the right display repeatedly, until the desired value shows. You increase or decrease the temperature in steps of 1 °C. 2. Press START to confirm the pre-selected value. If you do not press any button, the display flashes for a few seconds. The new preselected value is now stored and the display shows the actual value.
3. Close the centrifuge lid. 4. Restart the centrifuge.
The refrigeration starts operating if the pre-selected temperature is below the current temperature of the rotor chamber.
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Operation
3. 5. 6. Pre-Tempering the Centrifugation Chamber
Refrigerated centrifuges allow for pre-tempering, that is pre-warming or pre-cooling, the centrifugation chamber and the empty rotor before the centrifugation run starts. If necessary pre-temper your samples using proper equipment. The centrifuge is not intended to be used to pre-temper your samples. NOTICE Ventilated models cannot pre-temper the centrifugation chamber. In order to pre-temp the centrifuge and the unloaded rotor proceed as follows: 1. Press the SNOWFLAKE button.
An indicator above the SNOWFLAKE button indicates operation at the activated pretemp function.
2. Enter the desired value by pressing the up or down MINUS / PLUS buttons under the right display repeatedly, until the desired value shows. You increase or decrease the temperature in steps of 1 °C.
3. Press START. The rotor will be operated at optimal speed.
NOTICE When you press a different key than START you will quit the pre-temp function. NOTICE If you wish to change the temperature of your samples, please consider that the time required for temperature adjustment is prolonged. For critical applications you should take other precautions to ensure that the desired temperature is actually reached and maintained.
3. 5. 7. Changing the Settings During the Run
You can change the settings during the run as follows: 1. Press one of the three MINUS / PLUS button pairs in the control panel.
The current value will switch into the pre-selecting value mode. vwr.com I Instruction manual VWR Micro Star 21 47 / 87

Operation

2. Enter the new value as described above. 3. Press START. 4. The value is set and used immediately.

3. 6. Centrifugation

WA R N I N G

Damage to health from centrifuging explosive or flammable materials or substances. Do not centrifuge explosive or flammable materials or substances.

CAUTION

Mind the safety zone of minimum 30 cm around the centrifuge. Refer to "Safety Zone" on page 31. Persons and hazardous substances must be kept out of this safety zone while centrifuging.
Once the main switch has been turned on, the rotor has been properly installed, the setpoints have been set as explained in the previous section, and the centrifuge lid has been closed, you are ready to start.

Starting Centrifugation
Press START on the control panel. The centrifuge accelerates to the preset speed with the time display active.
The circulating indicators in the left display represent the spinning rotor.
The run display begins to count down from the pre-selected value. If the remaining run time is less then 1 minute, the remaining time is given in seconds.
In continuous operation hd (see "Continuous Operation" on page 46) the time display counts up. The elapsed run time is initially displayed in seconds. After one minute the display changes every minute.

Stopping Centrifugation
With Pre-selected Run Time If run time has been pre-selected, all you have to do is wait until the centrifuge terminates the run automatically. As soon as the speed drops to zero, the message END will appear in the display. By

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Operation

pressing OPEN, you can open the lid and remove the samples. You can also stop centrifugation manually at any time by pressing STOP.
Continuous Operation If you select continuous operation (refer to "Continuous Operation" on page 46), you will have to stop the centrifuge manually. 1. Press STOP on the control panel. 2. When the message END appears in the display, press OPEN to open the centrifuge
lid and remove the samples.

3. 7. Short-term Centrifugation
For short-term centrifuging, the centrifuge has a PULSE function. When you press and hold PULSE, spinning will start and continue until you release the key. The centrifuge accelerates to and brakes at maximum power. The pre-selected value is ignored.
NOTICE The centrifuge accelerates to maximum speed. The run time is initially displayed in seconds. After one minute the display changes every minute. After a short-term centrifugation run the set values are restored.

3. 8. Aerosol-tight Applications

Basic Principles
Make sure that the sample containers are well suited for the desired centrifugation process.
The temperature in ventilated centrifuges can reach 15 °C above room temperature.

CAUTION

Aerosol-tight rotors and tubes may only be opened in an approved safety workbench when centrifuging dangerous samples. Mind the maximum permissible load.

CAUTION Be sure to check all seals before starting any aerosol-tight applications.

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Operation

CAUTION

Prior to each use, the seals in the rotor need to be inspected in order to assure that they are correctly seated and are not worn or damaged. Damaged seals are to be replaced immediately. Replacement seals can be re-ordered as a spare part ("1. 6. Rotor Specifications" on page 17). When loading the rotor, ensure that the rotor lid closes securely. Damaged rotor covers are to be replaced immediately.

Replacing Seals
a

Top detail: Large seal in outer groove of rotor body; bottom detail: small seal in groove of rotor collar 1. Lubricate all seals. 2. Press the large seal in the outer groove of the rotor body (top detail). 3. Press the small seal into the groove of the rotor collar (bottom detail). 4. When loading the rotor, ensure that the rotor lid closes securely. 5. When the rotor lid is damaged or dulled it needs to be replaced.

Fill Level
The tubes are only to be filled to a level which ensures that the sample is unable to reach the top of the tube during centrifugation.

Nominal Volume 2.0 ml 1.5 ml others

Permissible Volume 1.5 ml 1.0 ml 2/3 of nominal volume

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Operation
Checking Aerosol Tightness
The aerosol tightness testing of the rotors and buckets depend on the microbiological test process in accordance with the EN 61010-2-020 Appendix AA. Whether or not a rotor is aerosol-tight depends primarily on proper handling. Check as needed to make sure your rotor is aerosol-tight. The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks, scratches and embrittlement are extremely important. Aerosol-tight applications are not possible if the lids are open. Aerosol tightness requires the correct operation when filling the sample vessels and closing the rotor lid.
Quick Test
As a quick test, it is possible to test the aerosol tightness of fixed-angle rotors using the following process: 1. Lubricate all seals lightly.
Always use the supplied grease when lubricating the seals. 2. Fill the cavities with approx. 10 ml of carbonated mineral water. 3. Close the rotor as explained in the handling instructions. 4. Shake the rotor vigorously using your hands.
This releases the carbonic acid gas, which is bound in the water, resulting in excess pressure. Do not apply pressure to the lid when doing so. Leaks can be detected by escaping water or the sound of escaping gas. Replace the seals if you detect any leaks. Then repeat the test. 5. Dry the rotor, rotor lid and the cover seal.
CAUTION The quick test is not suited for validating the aerosol tightness of a rotor. Check the seals and sealing surfaces of the lid thoroughly.
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Maintenance and Care
4. Maintenance and Care

4. 1. Cleaning Intervals
For the sake of personal, environmental, and material protection, you must clean and if necessary disinfect the shaker and its accessories on a regular basis.

4. 2. Basics
Use warm water with a neutral detergent that is suitable for use with the materials. If in doubt contact the manufacturer of the cleaning agent.
Use a soft cloth for cleaning.
Never use caustic cleaning agents such as soap suds, phosphoric acid, bleaching solutions or scrubbing powder.
Remove rotor and clean centrifugation chamber with a small amount of cleaning agent on a clean cloth.
Use a soft brush without metal bristles to remove stubborn residue.
Afterwards rinse with a small amount of distilled water and remove any remains with absorbent towels.
Use only cleaning and disinfecting agents with a pH of 6-8.
After thoroughly cleaning the rotors, they must be inspected for damage, wear and corrosion.
Make sure that sealing rings are still smooth, not brittle nor otherwise damaged. Some sealing rings are not autoclavable. Replace brittle or damaged sealing rings immediately. Refer to "Rotor Specifications" on page B-1 for details on sealing rings as spare parts.

CAUTION

Not rated procedures or agents could deteriorate the materials of the centrifuge and lead to malfunction. Refrain from using any other cleaning or decontamination procedure, if you are not entirely sure that the intended procedure is safe for the equipment. Use only cleaning agents that will not damage the equipment. In doubt contact the manufacturer of the cleaning agent. If still in doubt, contact VWR International.

CAUTION

Do not run any rotor or accessories with sign of damage. Ensure that the rotor, buckets and accessories are within their expected maximum number of cycles. It is recommend that you have rotors and accessories inspected yearly as part of your routine service to ensure safety.

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Maintenance and Care

4. 3. Cleaning
Clean as follows: 1. Clean rotor, buckets and accessories outside of the centrifugation chamber. 2. Separate rotor, buckets, lids, tubes and sealing rings to allow thorough cleaning. 3. Rinse rotor and all accessories with warm water and a neutral detergent that is suitable
for use with the materials. If in doubt contact the manufacturer of the cleaning agent. 4. Use a soft brush without metal bristles to remove stubborn residue. 5. Rinse rotor and all accessories with distilled water. 6. Place the rotors on a plastic grate with their cavities pointing down, to enable the
cavities to fully drain and dry. 7. Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet
at a maximum temperature of 50 °C. If drying boxes are used, the temperature must never exceed 50 °C. Higher temperatures could damage the material and shorten the lifetime of the parts. 8. Inspect the rotor and accessories for signs of damages. 9. After cleaning, treat the entire surface of aluminum parts including the cavities with corrosion protection oil (521-1769).

CAUTION

Before using any cleaning methods, users should check with the manufacturer of the cleaning agents that the proposed method will not damage the equipment.

CAUTION

Drive and door lock can be damaged by entering liquids. Do not allow liquids, especially organic solvents, to get on the drive shaft, the drive bearings or the centrifuge door locks. Organic solvents break down the grease in the motor bearing. The drive shaft could lock up.

CAUTION

Cutting injuries. Do not touch the condenser while cleaning the backside of the centrifuge. Cutting damage can occur if you touch the condenser due to their sharpness.

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Maintenance and Care
Cleaning the Filter Unit
Refrigerated centrifuges have a filter unit to protect the cooling device.
1. Pull the centrifuge to the edge of the table. 2. Pull the clip below the sucking grid and remove the filter unit completely by pulling it
down. 3. Remove the cumulated dust with a soft cloth. 4. Inserting the filter again the labeling Front must show to the front of the centrifuge. 5. Push the filter upwards into the slot until the clip locks in the bottom plate.
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Maintenance and Care

4. 4. Disinfection
You are responsible that the level of disinfection is achieved according to your requirements.

After disinfection:

1. Rinse the centrifuge and all affected accessories with water.

2. Allow to fully drain and dry.

3. After disinfecting, treat the entire surface of aluminum parts including the cavities with corrosion protection oil (70009824).

WA R N I N G

Do not touch infected parts. Hazardous infection is possible when touching the contaminated rotor and centrifuge parts. Infectious material can get into the centrifuge when a tube breaks or as a result of spills. In case of contamination, make sure that no one is put at risk. Disinfect the affected parts immediately.

CAUTION

Equipment can be damaged by inappropriate disinfection methods or agents. Make sure that the disinfection agent or the method will not damage the equipment. In doubt contact the manufacturer of the disinfection agent. Observe the safety precautions and handling instructions for the disinfection agents used.

4. 5. Decontamination
You are responsible that the level of decontamination is achieved according to your requirements.

After decontamination:

1. Rinse the centrifuge and all affected accessories with water.

2. Allow to fully drain and dry.

3. After decontaminating, treat the entire surface of aluminum parts including the cavities with corrosion protection oil (70009824).

WA R N I N G

Do not touch contaminated parts. Exposure to radiation is possible when touching the contaminated rotor and centrifuge parts. Contaminated material can get into the centrifuge when a tube breaks or as a result of spills. In case of contamination, make sure that no one is put at risk. Decontaminate the affected parts immediately.

CAUTION

Equipment can be damaged by inappropriate decontamination methods or agents. Make sure that the decontamination agent or the method will not damage the equipment. In doubt contact the manufacturer of the decontamination agent. Observe the safety precautions and handling instructions for the decontamination agents used.

vwr.com I Instruction manual VWR Micro Star 21 55 / 87

Maintenance and Care

4. 6. Autoclaving
Always disassemble all parts before autoclaving, e.g. lids need to be removed before autoclaving a bucket or rotor. If not stated otherwise on the parts themselves, all parts can be autoclaved at 121 °C for 20 min. Only exception is the hematocrit rotor at 134 °C for 20 min. Refer to "Rotor Specifications" on page 17 for details on rotors. Make sure that the necessary sterility is achieved according to your requirements. After autoclaving, treat the entire surface of aluminum parts including the cavities with corrosion protection oil (70009824).
CAUTION Never exceed the permitted temperature and duration when autoclaving.

NOTICE

No chemical additives are permitted in the steam.

4. 7. Service
VWR International recommends having the centrifuge and accessories serviced once a year by an authorized service technician. The service technician checks the following:
electrical equipment and connections
suitability of set-up site
centrifuge lid lock and safety system
rotor
fixation of rotor and drive shaft of the centrifuge
protective casing Before service, centrifuge and rotors should be thoroughly cleaned and decontaminated to ensure full and safe inspection can be completed. VWR International offers inspection and service contracts for this work. Any necessary repairs are performed for free during the warranty period and afterwards for a charge. That is only valid if the centrifuge has only been maintained by an authorized VWR International service technician. A validation of the centrifuge is recommended and can be ordered from customer service.
4. 8. Lifetime
The centrifuge is specified for a lifetime of 13 years. Decommissioning the centrifuge is suggested when this limit is reached. The lifetime of rotors, buckets and lids is based on cycles and specified individually for each rotor in the chapter "Rotor Specifications" on page 17. Other accessories are not limited by a specific lifetime and need only be replaced when damaged or worn.
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Maintenance and Care

4. 9. Shipping
Before shipping the centrifuge:
The centrifuge must be clean and decontaminated. You must confirm the decontamination with a decontamination certificate.

WA R N I N G

Before shipping the centrifuge and accessories you must clean and, if necessary, disinfect or decontaminate the full system. If you are not sure, consult with VWR International customer service.

4. 10. Storage

Before storing the centrifuge and the accessories it must be clean and if necessary disinfected and decontaminated. Centrifuge, rotors, buckets and accessories have to be fully dry before storage.

Keep the centrifuge in a clean, dry and dust-free location.

Do not store the centrifuge in direct sunlight.

WA R N I N G

When you remove the centrifuge and accessories from use, clean and if necessary disinfect or decontaminate the full system. If you are not sure speak to the VWR International customer service.

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Troubleshooting
5. Troubleshooting
5. 1. Mechanical Emergency Lid Release
During a power failure, you will not be able to open the centrifuge lid with the regular electric lid release. A mechanical override is provided to allow sample recovery in the case of an emergency. However, this should be used only in emergencies and after the rotor has come to a complete stop. Always wait until the rotor has come to a stop without braking. The brake does not work when there is no power. The braking process lasts much longer than usual. Proceed as follows: 1. Wait until the rotor has stopped. This can take several minutes. Use the view port
for visual confirmation. 2. Pull out the power supply plug. 3. Insert a 3 inch long wire (e.g. a staple) into the hole above the control panel. 4. Press the centrifuge door down gently. Push the wire further into the hole until you hear
and feel the door latch unlocking. 5. Remove the wire from the hole and open the centrifuge lid.
The samples can be removed.

View port; Emergency lid release Figure 7: Emergency Lid Release
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Troubleshooting

6. Reconnect the centrifuge once the power has been restored. 7. Switch on the centrifuge.

WA R N I N G

5. 2. Ice Formation
Warm humid air in combination with a cold centrifugation chamber can lead to formation of ice. To remove the ice from the centrifugation chamber, proceed as follows: 1. Open the centrifuge lid. 2. Remove the rotor. Refer to "How to remove a rotor" on page 38. 3. Let the ice melt.
NOTICE Do not use any sharp tools, aggressive liquids or fire to fasten the melting process. If necessary use warm water to speed up the melting process. 4. Remove the water from the centrifugation chamber.
5. 3. Troubleshooting by Guide

NOTICE

If an error message shows that is not listed in this table, a service technician must be contacted.

Error
Display remains dark.
Display fails briefly.

Description

Solutions

The drive stops. The centrifuge runs down without being braked. The centrifuge lid cannot be opened.

No mains connection. Is the centrifuge turned on? Check the mains connection. If the error message still shows, contact a service technician.

The drive stops. The centrifuge runs down without being braked.

Mains connection interrupted for some seconds, · Turn off mains switch. · Check whether the mains power cord is connected properly · Restart the centrifuge.

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Troubleshooting

Error
The centrifuge lid cannot be opened.
Display oP appears although lid is closed. Lid
E-01 - E-13
E-14
E-15-E-16
E-22 - E-23

Description
Pressing OPEN has no effect.
Exceptionally running noise.
Centrifuge does not start.

Solutions
Centrifuge lid is not correctly engaged or warped. · Check if mains connection is working and the instrument is
switched on (display is lit). · If this is unsuccessful, you may open the centrifuge lid
using the mechanical emergency lid release ("Mechanical Emergency Lid Release" on page 58)
Imbalance · Stop the centrifuge. Press STOP or unplug mains power cord. · Wait until the centrifuge comes to a complete stop. · Check whether the rotor is properly loaded. · Check whether a broken tube, damage to the rotor or motor is
responsible for the run noise. If the error message still shows, contact a service technician.
Centrifuge lid not properly closed. Open the centrifuge lid and repeat locking procedure. If the error message still shows, contact a service technician.

Rotor stops with deceleration to standstill.

Centrifuge lid was opened manually during the run. Close centrifuge lid immediately Rotor stops with deceleration to standstill. For further centrifugation, you have to switch the instrument off and switch it on again.

Rotor stops with deceleration to standstill. The centrifuge cannot be operated.

Internal program error Switch the instrument off and on again. If the error message still shows, contact a service technician.

Rotor stops with deceleration to standstill. The centrifuge cannot be operated.

Overtemperature in the centrifuge chamber. Switch the centrifuge off and turn it on again after approx. one minute. If the error message still shows, contact a service technician.

Rotor stops with deceleration to standstill. The centrifuge cannot be operated.

Temperature measurement error. Switch the instrument off and on again. If the error message still shows, contact a service technician.

Rotor stops with deceleration to standstill. The centrifuge cannot be operated.

Error in speed entry. Switch the instrument off and on again. The display shows BR and a countdown from 100 - 0. If the error message still shows, contact a service technician.

vwr.com I Instruction manual VWR Micro Star 21 60 / 87

Troubleshooting

Error
E-24
E-29 E-31
E-33
E-36 E-41 - E-56

Description

Solutions

The centrifuge cannot be operated.

Wrong status information from the lid latch. · Switch the instrument off and on again. · After re-switching on, the display shows Lid FAiL. · If the centrifuge lid has been already opened, the display
shows CLOSE Lid. Close the lid. · The centrifuge tries to open the lid to switch for starting the
normal operation mode. If the error message still shows, contact a service technician.

Motor does not start

Motor or rotor blocked · Switch instrument off and on again using the mains switch. · Open the centrifuge lid. · Check whether the rotor can turn freely. If the error message still shows, contact a service technician.

Rotor stops without deceleration to standstill or does not start.

Overtemperature in the motor · Turn instrument off and unplug mains power cord. · Check and clean the venting slots if necessary and
respectively the filter unit of the cooled centrifuge. · After approx. 60 minutes you can restart the instrument. Observe the maximum permissible environmental temperature. If the error message still shows, contact a service technician.

Rotor stops with deceleration to standstill.

Overpressure in the refrigeration system · Turn instrument off and unplug mains power cord. · Check and clean the venting slots if necessary and
respectively the filter unit of the cooled centrifuge. · After approx. 60 minutes you can restart the instrument. Observe the maximum permissible environmental temperature. If the error message still shows, contact a service technician.

Rotor stops with deceleration to standstill. The centrifuge cannot be operated.

Overcurrent or error in current measurement Switch the instrument off and on again. If the error message still shows, contact a service technician.

Rotor stops with deceleration to standstill. The centrifuge cannot be operated.

Internal program error Switch the instrument off and on again. If the error message still shows, contact a service technician.

vwr.com I Instruction manual VWR Micro Star 21 61 / 87

Troubleshooting

Error
E-60

Description
Rotor stops with deceleration.

Table 32: Troubleshooting

Solutions
Insufficient temperature in the refrigeration unit. · Stop the centrifugation run. · Open the centrifuge lid and defrost the chamber. Never touch
the chamber directly with your hands you may freeze up. · After approx. 60 minutes you can restart the instrument.
Observe the maximum permissible ambient temperature · If a strong ice sheet is present in the internal chamber, be sure
to remove all condensate after defrosting. If the error message still shows, contact a service technician.

5. 3. 8. Information for the Customer Service
If you need to contact customer service, please provide the order no. and the serial no. of your device. This information can be found on the back near the inlet for the power supply cable.

To identify the software version: Keep STOP pushed when switching on the centrifuge. In the display all segments will be lit. Subsequently, the following entries will be displayed for 5 seconds each:

Software number SOFT

063

Software version

_02

NV-RAM number EEPRO

558

NV-RAM version

_01

Cycle counter

CYCLE

001

This translates into the following information:
» Software 0633 Version 02 » NV-RAM 5583 Version 01 » Cycles completed 125

NOTICE

The values shown above are just examples.

vwr.com I Instruction manual VWR Micro Star 21 62 / 87

Technical service

Troubleshooting

Web Resources
Visit the VWR website at www.vwr.com for:
Complete technical service contact information Access to the VWR Online Catalogue, and information about accessories and related
products Additional product information and special offers
Contact us For information or technical assistance contact your local VWR representative or visit. www.vwr.com.

Warranty
VWR warrants that this product will be free from defects in material and workmanship for a period of two (2) years from date of delivery. If a defect is present, VWR will, at its option and cost, repair, replace, or refund the purchase price of this product to the customer, provided it is returned during the warranty period. This warranty does not apply if the product has been damaged by accident, abuse, misuse, or misapplication, or from ordinary wear and tear. If the required maintenance and inspection services are not performed according to the manuals and any local regulations, such warranty turns invalid, except to the extent, the defect of the product is not due to such non-performance.
Items being returned must be insured by the customer against possible damage or loss. This warranty shall be limited to the aforementioned remedies. IT IS EXPRESSLY AGREED THAT THIS WARRANTY WILL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND IN LIEU OF THE WARRANTY OF MERCHANTABILITY.

Compliance with local laws and regulations
The customer is responsible for applying for and obtaining the necessary regulatory approvals or other authorisations necessary to run or use the Product in its local environment. VWR will not be held liable for any related omission or for not obtaining the required approval or authorisation, unless any refusal is due to a defect of the product.

vwr.com I Instruction manual VWR Micro Star 21 63 / 87

Troubleshooting
Equipment disposal
This equipment is marked with the crossed out wheeled bin symbol to indicate that this equipment must not be disposed of with unsorted waste. Instead it's your responsibility to correctly dispose of your equipment at lifecycle -end by handling it over to an authorized facility for separate collection and recycling. It's also your responsibility to decontaminate the equipment in case of biological, chemical and/or radiological contamination, so as to protect from health hazards the persons involved in the disposal and recycling of the equipment. For more information about where you can drop off your waste of equipment, please contact your local dealer from whom you originally purchased this equipment. By doing so, you will help to conserve natural and environmental resources and you will ensure that your equipment is recycled in a manner that protects human health. Thank you
vwr.com I Instruction manual VWR Micro Star 21 64 / 87

6. Chemical Compatibility
Chemical Compatibility

Chemical Compatibility
VitonTM TygonTM Titanium Stainless Steel Silicone Rubber Rulon ATM, TeflonTM Polyvynil Chloride Polysulfone Polypropylene Polyethylene Polythermide Polyester, Glass Thermoset Polycarbonate Polyallomer PET¹, PolyclearTM,Clear CrimpTM Nylon NorylTM Neoprene Glass EPDM rubber DelrinTM Composite Carbon Fiber/Epoxy Polyurethane Rotor Paint Cellulose Acetate Butyrate Buna N Anodic Coating for Aluminum Aluminium
MATERIAL
vwr.com I Instruction manual VWR Micro Star 21 65 / 87

CHEMICAL

2-mercaptoethanol S S U / S M S / S U S S U S S / S S S S U S S S S S S

Acetaldehyde

S / U U / / / M/ U / / / MU U U MM/ MS U / S / U

Acetone

MS U U S U MS S U U S U S U U U S S U U S MMS U U

Acetonitrile

S S U / S MS / S S U S U MU U / S MU U S S S S U U

AlconoxTM

U U S / S S S / S S S S S S MS S S S S S S S S S S U

Allyl Alcohol

/ / / U / / S / / / / S / S S MS S S / MS / / S / /

Aluminum Chloride U U S S S S U S S S S M S S S S / S S S S S M U U S S

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Formic Acid (100%)

/ S MU / / U / / / / U / S MU U S S / U S / U S / U

Ammonium Acetate S S U / S S S / S S S S S S S U / S S S S S S S S S S

Ammonium Carbonate

MS U S S S S S S S S S S S U U / S S S S S S MS S S

Ammonium Hydroxide (10%)

U U S U S S MS S S S S /

S U MS S S S S S S S S MS

Ammonium Hydroxide (28%)

U U S U S U MS S S S S U S U MS S S S S S S S S MS

Ammonium U U U U S U MS / S / S U S U U S S S / MS S S S / U
Hydroxide (conc.)

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Ammonium Phosphate

U / S / S S S S S S S S / S S M/ S S S S S S MS S S

Ammonium Sulfate U M S / S S U S S S S S S S S S / S S S S S S U S S U

Amyl Alcohol

S / MU / / S S / M/ S / MS S S S M/ / / U / S / M

Aniline

S S U U S U S MS U U U U U U U / S MU U S S S S U S

Sodium Hydroxide U / MS S S / / S MS S / S MMS S S S S S MS S / U
(<1%)

Sodium Hydroxide U / MU / / U / MMS S U S U U S S S S S S MS S / U
(10%)

Barium Salts

MU S / S S S S S S S S S S S M/ S S S S S S MS S S

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL Benzene Benzyl Alcohol Boric Acid Cesium Acetate Cesium Bromide Cesium Chloride Cesium Formate Cesium Iodide Cesium Sulfate Chloroform
S M
U /

S S U U S U MU S U U S U U U MU MU U U S U U S U S S / U U / / MM/ M/ S U U U U U U U / MS M/ S / S U S S MS S U S S S S S S S S S U S S S S S S S S S S M/ S / S S S / S S S S / S S / / S S S S S S MS S S MS S / S S S / S S S S S S S / / S S S S S S MS S S MS S U S S S / S S S S S S S / / S S S S S S MS S S MS S / S S S / S S S S S S S / / S S S S S S MS S S MS S / S S S / S S S S S S S / / S S S S S S MS S S MS S / S S S / S S S S S S S / / S S S S S S MS S S U U U U S S MU S U U MU MU U U MMU U S U U U MS
Satisfactory Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual conditions of use Unsatisfactory, not recommended Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL Chromic Acid (10%) Chromic Acid (50%) Cresol Mixture Cyclohexane Deoxycholate Distilled Water Dextran Diethyl Ether
S M
U /

U / U U S U U / S S S U S S MU MS S U MS MU S S S
U / U U / U U / / / S U U S MU MS S U MS / U M/ S
SSU/ / / S/ SUUUUUU/ / UU/ USSSSUS S S S / S S S U S U S S U U U MS MU MMS U MMU S SSS/ SSS/ SSSSSSS/ / SSSSSSSSSS SSSSSSSSSSSSSSSSSSSSSSSSSSS MS S S S S S / S S S S S S S S S S S S S S S MS S S S S U U S S S U S U U S U U U U U U U U U S S S S MU
Satisfactory Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual conditions of use Unsatisfactory, not recommended Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Diethyl Ketone

S / U U / / M/ S U / S / MU U U MM/ U S / / S U U

Diethylpyro-
carbonate

S S U / S S S / S S U S U S U / / S S S MS S S S S S

Dimethylsulfoxide S S U U S S S / S U S S U S U U / S S U U S S S S U U

Dioxane

MS U U S S MMS U U S U MU U / MMMU S S S S U U

Ferric Chloride

U U S / / / MS / M/ S / S / / / S S / / / MU S / S

Acetic Acid (Glacial)

S S U U S S U MS U S U U U U U MS U MU S U U S / U

Acetic Acid (5%) S S M S S S M S S S S S M S S S S S S S M S S M S S M

Acetic Acid (60%) S S U U S S U / S M S U U M U S M S M S M S M U S M U

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL Ethyl Acetate Ethyl Alcohol (50%) Ethyl Alcohol (95%) Ethylene Dichloride Ethylene Glycol Ethylene Oxide Vapor Ficoll-HypaqueTM
S M
U /

MMU U S S MMS S U S U MU U / S S U U S MMS U U S S S S S S MS S S S S U S U S S S S S S S S MS MU
S S S U S S MS S S S S U S U / S S S MS S S U S MU
S / U U / / S M/ U U S U U U U U U U / U S U / S / S S S S S S S S S S S S S / S U S S S S S S S S MS MS S / U / / U / / S U / S / S M/ / S S S U S U S S S U MS S / S S S / S S S S / S S / S S S S S S S MS S S Satisfactory Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual conditions of use Unsatisfactory, not recommended Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Hydrofluoric Acid U U U M/ / U / / U U S / S MU S S S S MS U U U / /
(10%)

Hydrofluoric Acid U

U U U U S U U U S S MMS U U U /

(50%)

Hydrochloric Acid U

U U M/

U MU U MU U U /

USUUU/

(conc.)

Formaldehyde (40%)

MMMS S S S MS S S S MS S S U S S MS S S MS MU

Glutaraldehyde

SSSS/ / S/ SSSSSSS/ / SSS/ / SSS/ /

Glycerol

MS S / S S S S S S S S S S S S / S S S S S S S S S S

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Guanidine Hydrochloride

UUS/ SSS/ SSSSSSS/ / SSSSSSUSSS

Haemo-SolTM

SSS/ / / S/ SSSSSSS/ / SSSSSSSSSS

Hexane

S S S / S S S / S S U S U MU S S U S S MS U S S U S

Isobutyl Alcohol / / M U / / S S / U / S U S S M S S S / S S S / S / S

Isopropyl Alcohol M M M U S S S S S U S S U S U M S S S S S S S M M M S

Iodoacetic Acid

S S M/ S S S / S MS S MS S / MS S S S S MS S MM

Potassium Bromide U S S / S S S / S S S S S S S S S S S / S S S M S S S

Potassium Carbonate

MU S S S S S / S S S S S S U S S S S S S S S S S S S

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Potassium Chloride

USS/ SSSSSSSSSSS/ SSSSSSSUSSS

Potassium Hydroxide (5%)

SSSS/

S U S S S S S S S MU MS U

Potassium U U MU / / M/ MS S / U MU U U S M/ MU / U U / U
Hydroxide (conc.)

Potassium Permanganate

S S S / S S S / S S S U S S S M/ S MS U S S MS U S

Calcium Chloride M U S S S S S S S S S S S S M S / S S S S S S M S S S

Calcium Hypochlorite

M/ U / S MMS / M/ S / S MS / S S S MS MU S / S

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL Kerosene Sodium Chloride (10%) Sodium Chloride (sat'd) Carbon Tetrachloride Aqua Regia Solution 555 (20%)
S M
U /

S S S / S S S U S MU S U MMS / S/ SSSSSS/ / / SSSSS/

MMMS S U S S U S S S S S / S S M/ S

U / S U S S S / / / / S S S S S / S S / S / S S M/ S

U U MS S U MU S U U S U MU S S MMS MMMMU S S U/ UU/ / U/ / / / / UUUUUUU/ / / / / S/ M SSS/ / / S/ SSSSSSS/ / SSS/ SSSSSS

Satisfactory Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual conditions of use Unsatisfactory, not recommended Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL Magnesium Chloride Mercaptoacetic Acid Methyl Alcohol Methylene Chloride Methyl Ethyl Ketone MetrizamideTM
S M
U /

MS S / S S S S S S S S S S S S S S S S S S S MS S S
U S U / S MS / S MS U U U U / S U U S MS U S S S S S S S U S S MS S S S S U S U MS S S S S S S MS MU U U U U MS S U S U U S U U U U U MU U U S S MU S U
S S U U S S MS S U U S U S U U U S S U U S S S S U U MS S / S S S / S S S S / S S / / S S S S S S MS S S Satisfactory Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual conditions of use Unsatisfactory, not recommended Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Lactic Acid (100%)

/ / S / / / / / / MS U / S S S MS S / MS MS S / S

Lactic Acid (20%) / / S S / / / / / M S M / S S S S S S S M S M S S / S

N/Butyl Alcohol S / S U / / S / / S M / U S M S S S S M M S M / S / S

N/Butyl Phthalate S S U / S S S / S U U S U U U M / U U S U S M M S U S

N, S S S U S MS /
N-Dimethylformamide

SSUSUSUU/

S S U U S MS S S U

Sodium Borate

MS S S S S S S S S S U S S S S / S S S S S S MS S S

Sodium Bromide

U S S / S S S / S S S S S S S S / S S S S S S MS S S

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Sodium Carbonate MU S S S S S S S S S S S S U S S S S S S S S S S S S
(2%)

Sodium Dodecyl Sulfate

SSS/ SSS/ SSSSSSS/ SSSSSSSSSSS

Sodium

U U MS S MU S S MS S S MS S S S MS S S MU S MS

Hypochlorite (5%)

Sodium Iodide

MS S / S S S / S S S S S S S / / S S S S S S MS S S

Sodium Nitrate

SSS/ SSSSSSSSSSSS/ SSSSSUSSSS

Sodium Sulfate

U S S / S S S S S S S S S S S S S S S S S S S MS S S

Sodium Sulfide

S / S S / / / S / / / S S S U U / / S / / / S S M/ S

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL Sodium Sulfite Nickel Salts Oils (Petroleum) Oils (Other) Oleic Acid Oxalic Acid Perchloric Acid (10%) Perchloric Acid (70%)

S S S / S S S S MS S S S S S M/ S S S S S S S S S S U S S S S S / S S S / / S S S S / S S S S S S MS S S S S S / / / S U S S S S U U MS MU U S S S U S S S S S / S / / / S MS S S S U S S S S U S S S S / S S MS S / U S S S U U S U S S MS S S S S S S S S MU S MM U U MS S S U S S S S S U S U S S S S S S S S U MS S
U / U / S U U / S MM/ / MU MS MM/ MS U / S / S
U U U / / U U / S U MU U MU U U MMU MS U U S U S

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Phenol (5%)

U S U / S MM/ S U MU U S U MS MS U U S U MMMS

Phenol (50%)

U S U / S U M/ S U MU U U U U S U MU U S U U U MS

Phosphoric Acid (10%)

U U MS S S U S S S S U / S S S S S S S S S U MU S S

Phosphoric Acid (conc.)

U U MM/ / U S / MS U U MMS S S MS MS U MU / S

Physiologic Media

SSSSSSSSSSSSSSSSSSS

(Serum, Urine)

Picric Acid

S S U / S MS S S MS U S S S U S S S S U S U MS MS

Pyridine (50%)

U S U U S U U / U S S U U MU U / U S MU S S U U U U

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Rubidium Bromide M S S / S S S / S S S S S S S / / S S S S S S M S S S

Rubidium Chloride M S S / S S S / S S S S S S S / / S S S S S S M S S S

Sucrose

MS S / S S S S S S S S S S S S S S S S S S S S S S S

Sucrose, Alkaline M S S / S S S / S S S S S S U S S S S S S S S M S S S

Sulfosalicylic Acid U U S S S S S / S S S U S S S / S S S / S S S U S S S

Nitric Acid (10%) U S U S S U U / S U S U / S S S S S S S S S M S S S S

Nitric Acid (50%) U S U M S U U / S U S U U M M U M M M S S S U S S M S

Nitric Acid (95%) U / U U / U U / / U U U U M U U U U M U U S U S S / S

Hydrochloric Acid U U MS S S U /
(10%)

S S S U U S U S S S S S S S S U MS S

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Hydrochloric Acid UUUUSUU/
(50%)

S MS U U MU U S S S S MS MU U MM

Sulfuric Acid (10%)

MU U S S U U / S S MU S S S S S S S S S S U U U S S

Sulfuric Acid (50%)

MU U U S U U / S S MU U S U U MS S S S S U U U MS

Sulfuric Acid (conc.)

MU U U / U U M/ / MU U S U U U MS U MS U U U / S

Stearic Acid

S / S / / / S MS S S S / S S S S S S S S S MMS S S

Tetrahydrofuran S S U U S U U M S U U S U U U / M U U U U S U S S U U

Toluene

S S U U S S MU S U U S U U U S U MU U U S U S U U M

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Trichloroacetic Acid

U U U / S S U MS U S U U S M/ MS S U U S U U U MU

Trichloroethane

MU /

SUUUUUUUUUSU/

Trichloroethylene / / U U / / / U / U / S U U U U U U U U U S U / U / S

Trisodium Phosphate

/ / / S / / M/ / / / / / S / / S S S / / S / / S / S

Tris Buffer (neutral pH)

USSSSSS/ SSSSSSSSSSSSSSSSSSS

Triton X/100TM

SSS/ SSS/ SSSSSSSSSSSSSSSSSSS

Urea

S / U S S S S / / / / S S S MS S S S / S S S MS / S

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

Chemical Compatibility

CHEMICAL

Hydrogen Peroxide U U MS S U U /
(10%)

S S S U S S S MU S S S S S S MS U S

Hydrogen Peroxide S

(3%)

S S S S S S S S MS S S S S S S S S S

Xylene

S S U S S S MU S U U U U U U MU MU U U S U MS U S

Zinc Chloride

UUSSSSUSSSSSSSSSSSSSSSSUSSS

Zinc Sulfate

USS/ SSSSSSSSSSSSSSSSSSSSSSS

Citric Acid (10%) M S S M S S M S S S S S S S S S M S S S S S S S S S S

Satisfactory

Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.; suggest testing under actual

conditions of use

Unsatisfactory, not recommended

Performance unknown; suggest testing, using sample to avoid loss of valuable material

1 Polyethlyeneterephtalate

NOTICE Chemical resistance data is included only as a guide to product use. Because no organized chemical compatibility data exists for materials under the stress of

centrifugation, when in doubt we recommend pretesting sample lots.

Index
Symbole
10 x 5 mL Rotor 23 24 x 1.5/2.0 mL Rotor 17 36 x 0.5 mL Rotor 21
A
Aerosol-tight Applications 49 Audible Alarm 35 Autoclaving 56
C
Care 52 Centrifugation 48 Chemical Compatibility 65 Clamps 15 Cleaning 53 Cleaning Filter Unit 54 Cleaning Intervals 52 Control Panel 34
D
Decontamination 55 Directives 15 Disinfection 55 Disposal 57 Dual Row 18 x 2.0/0.5 mL Rotor 19
E
Entering Centrifugation Parameters 44
H
How to install a rotor 37 How to Operate a Rotor 37 How to remove a rotor 38

I
Ice Formation 59 Information for the Customer Service 62 Intended Use 6 Items Supplied 29
L
List of Centrifuges 12 List of Rotors 12 Load the Rotor 41 Location 30
M
Mains Connection 33 Mains Supply 16 Maintenance 52 Maximum Loading 42 Mechanical Emergency Lid Release 58
O
Open / Close the Centrifuge Lid 36 Operation 34
P
PCR 4 x 8 Rotor 27 PCR 8 x 8 Rotor 25 Power on / off 35
R
Refrigerants 16 Rotor Specifications 17
S
Safety Instructions 7 Service 56 Shipping 57 Short-term Centrifugation 49 Signal Words and Symbols 6

Standards 15 Storage 34
T
Technical Data 13 Technical Specifications 12 Transport and Set Up 29 Troubleshooting 58 Troubleshooting by Guide 59
U
Unpacking 29

Avantor One Radnor Corporate Center Building One, Suite 200 100 Matsonford Road Radnor, PA 19087 1 610 386 1700

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