65 Filtration Devices Table of Contents Filtration Device Portfolio Introduction 66 Minisart Standard Selection Guide 67 Minisart PP Standard Syringe Filter 68 Minisart NML Standard Syringe Filters 74 Medical Device CE-Minisart Syringe Filters 80 Claristep Syringeless Filtration 83 Sartolab P20 and Sartolab P20 Plus 87 Sartolab RF | BT 91 ...
65 Filtration Devices Table of Contents Filtration Device Portfolio Introduction 66 Minisart® Standard Selection Guide 67 Minisart® PP Standard Syringe Filter 68 Minisart® NML Standard Syringe Filters 74 Medical Device CE-Minisart® Syringe Filters 80 Claristep® Syringeless Filtration 83 Sartolab® P20 and Sartolab® P20 Plus 87 Sartolab® RF|BT 91 NEW! Sartolab® RF 50 94 NEW! Sartolab® Multistation 96 Sartoclear Dynamics® Lab P15 98 NEW! Sartoclear Dynamics® Lab V 100 Chemical Compatibility 104 66 Filtration Devices Introduction Introduction Syringe filters are used for many routine preparation steps in laboratories all over the world. They are convenient, ready-to-use disposables for sterile filtration of liquids and removal of particles from solutions and gases. Depending on the reagents filtered, syringe filters have to fulfill certain requirements to best serve customer's application. Sartorius offers Minisart® syringe filters and filters optimized for a wide range of relatively large volumes. The filters are reliably remove particles with no leakage. If you need to rely on the quality of your filtrate whether it needs to be sterile prior to use or particulate-free before analysis fieldproven, high-quality Sartorius filter syringes are the No. 1 choice for reliable, convenient preparation steps. Sartorius has developed a new, easy-to-use and straightforward filtration setup. The manually operated Claristep® Filtration System consisting of a station and filter units offers a novel way for clarifying your samples prior to analysis. Claristep® Filter units are processed without syringe and are made of the purest materials. Another major benefit is that the contact time of the samples with the filters and the caps is extremely short, ensuring optimal, contamination-free results. The Claristep® Station consists of a base, a lid and an exchangeable tray for easy and accurate positioning of sample vials and Claristep® Filter units. Our Product Range For clarification and sterilization of liquids, filtration is the optimal method. It removes microorganisms and particles reliably, without any effects on the ingredients due to adsorption or decomposition. For optimal results, Minisart® NML Standard syringe filters with an MBS housing provide a choice of membranes with pore sizes ranging from 0.1 m to 5 m for high flow rates and the low adsorption characteristics. The effective filtration area of 6.2 cm2 for the fast filtration is the largest among premium syringe filters available, and the MBS housing is color- coded for easy pore size identification. For a list of the types offered, please see page 74. Elimination of particles from your samples prior to HPLC or other chromatographic analysis is essential in order to maintain the integrity of your chromatography column and to maximize its operating lifetime. Minisart® PP Standard syringe filters optimized for sample preparation consist of a polypropylene housing and membrane components featuring maximum chemical compatibility and minimum extractables to ensure excellent results. Due to the typical range of volumes from less than 1 mL to 100 mL, these filters are available in three different diameters with an effective filtration area of 0.07 cm2, 1.7 cm2 and 4.8 cm2. For a selection guide, please see page 67.. The Sartorius medical device CE-Minisart® syringe filter with a hydrophilic (surfactant-free) cellulose acetate and hydrophobic polyt etrafluoroethylene (PTFE) are the perfect choice for pharmacy admixture applications like sterile filtration and|or clarification of low volume solutions in a laboratory environment before use for patient care. The CE-Minisart® syringe filters are manufactured by Sartorius in a facility whose Quality Management System is certified for compliance with EN ISO 13485 (see page 80). Claristep® syringeless filter units with RC membranes are optimized for solvents and aqueous solutions. They provide maximum chemical compatibility and exceptionally low non-specific binding of analytes. Sartolab® vacuum filtration devices with 0.1µm and 0.22µm PES membranes for convenient filtration of 150mL up to 1 L are ready to use and sterile. Sartolab® RF is a complete syst em that includes a receiver flask. Sartolab® BT Sterile is a bottle top filter without a receiver flasks. This enables customers to use a receiver bottle of their choice and to even expand filtration capacity, depending on the particle load of the filtered liquid by filling more than one receiver flask. Sartolab® 150V is a disposable vacuum filter with a pleated 0.22µm PES membrane, which is suitable for up to 15 L of liquid. Sartolab® P20 pressure filtration devices with a 0.2µm and 0.45µm PES membrane are available with or without a glass fiber prefilter, depending on your needs. Sartolab P20 is designed for up to 3 L volumes and can also be used in-line. The polycarbonate housing and membrane components are ideal for filtering liquids. The glass fiber prefilter types are ideal for filtering environmental samples that have a high particle load prior to analyzing such samples. ----Typical Applications for Filtration Devices Sterile filtration of liquids and gases with virtually no effect on the ingredients Particle removal from liquids and gases prior to downstream processes Venting of vials, bottles, containers, bags and bioreactors and fermenters Removal of precipitates and coagulates from solutions prior to use Minisart® Standard Syringe Filters are not for medical use. Filtration Devices Minisart® Syringe Filters 67 Minisart® Standard Selection Guide Please refer to Minisart® RC, NY or SRP for the highest chemical compatibility on page 71. Please refer to Minisart® NML or Minisart® High Flow on page 74. Sample Composition Aqueous Aqueous|Solvents All Aqueous Solutions All Aqueous Solutions Buffers, Protein Analysis Tissue Culture Media Aqueous|Solvent Solvent Mixtures| Mixtures|Solvents Solvents Solvents|Gases| Acids|Bases CA Cellulose Acetate PES Polyethersulfone RC Regenerated Cellulose Hydrophilic NY Polyamide, Nylon PTFE Polytetrafluoro ethylene Hydrophobic Pore Sizes Sterilization Sample Preparation|Clarification|Particle Removal Prefiltration Small Bacteria Mycoplasma Colloids >0.1 UHPLC, etc. (Columns <3µm Particles) Bacteria HPLC, etc. (Columns >3µm Particles) Particles Particles Yeast Cells Particles Yeast Cells Particles Yeast Cells Platelets Large Particles Cells Glass Prefilter Glass+Membrane Highly Particle-laden Samples 0.1µm 0.2µm 0.45µm 0.65µm 0.8µm 1.2µm 5µm GF (Glass Fiber) Sample Volumes 1mL to 200mL 28mm for up to 200mL 1mL to 100mL 25mm for up to 100mL 0.5mL to 15mL 15mm for up to 15mL 0.05mL to 1mL 4mm for up to 1mL 68 4mm packages are color-coded Male Spike Outlet Male Luer Slip Outlet Minisart® RC 15mm Minisart® NY 15mm Minisart® SRP 15mm Filtration Devices Minisart® Syringe Filters Minisart® PP Standard Syringe Filter Sample Preparation for Analytics Reliable Removal of Particles from Liquids and Gases Particle removal by filtration before analysis substantially increases the lifetime of your columns. Minisart® RC is optimized for aqueous liquids and solvents and is compatible with DMSO, other amides, ketones, esters and ethers. Minisart® NY is exceptionally pure compared with other common polyamide (=nylon) filters and competitor products. For this product raw materials are used which do not interfere with standard analytical methods. Our coating-free hydrophobic PTFE membrane used in Minisart® SRP is suitable for venting applications. ---Minisart® Features Low adsorption of analytes Maximum chemical compatibility Minimum extractables Minisart® RC 25mm Minisart® NY 25mm Minisart® SRP 25mm Filtration Devices Minisart® Syringe Filters 69 HPLC Certification mAU 70 60 50 40 30 20 10 0 -10 0 mAU 70 60 50 40 30 20 10 0 -10 0 mAU 70 60 50 40 30 20 10 0 -10 0 Methanol 10 20 30 40 50 60 min Acetonitrile 10 20 30 40 50 60 min Water 10 20 30 40 50 60 min Filtered with Minisart® SRP Filtered with Minisart®NY Filtered with Minisart® RC Unfiltered Methanol Filtered with Minisart® SRP Filtered with Minisart®NY Filtered with Minisart® RC Unfiltered Acetonitrile Filtered with Minisart® PES Filtered with Minisart® NML Unfiltered Water HPLC Procedure Column C18: 250×4.6mm, Flow Rate: 1mL/min, Wavelength: 220nm HPLC Injection Volume: 20µL, Analysis Time: 65min, Temperature: 40°C, Mobile Phases: A) Acetonitrile| B) Water, Gradient: Hold 60% A for 10min, 60% to 95% A in 20min, 95% to 100% A in 35min 70 Minisart® with Polypropylene Housing CFihltarapttieorn Devices Minisart® Syringe Filters Specifications Minisart® RC|SRP|NY|PES with 4|15|25mm Membrane Filtration Area Housing material Polypropylene (PP) Membranes RC = Regenerated Cellulose|NY = Polyamide| SRP = Hydrophobic PTFE = Polytetrafluoroethylene|PES = Polyethersulfone| PES - = hydrophobic PES Glass fiber prefilter NY Plus: Ultrapure quartz, 0.7µm particle retention Max. operating pressure 4.5bar|65psi Minisart® PES - : 2.0bar|29psi (IN - OUT) or 0.5 bar|7psi (OUT - IN) Housing burst pressure 7bar|102psi Max. temperature 60°C Sterilization Non-sterile Minisart® can be autoclaved or sterilized by ethylene oxide (EO) Minisart® Membrane Types Non-sterile packs: 50 (K), 200 (S), 500 (Q), 1000 (R)|sterile packs: individually packaged, 50 (ACK) Bubble point () RC 0.2µm K | S | Q | R With water 3.0bar|44psi RC 0.2µm ACK With water 4.6bar|67psi RC 0.45µm K | S | Q | R With water 2.0bar|29psi ---Flow rate (() mL/min), 4mm = 0.07cm2 filter area|Hold-up volume1: 10µL For water at 1bar 0.5 For methanol at 1bar 1.5 For air at 0.1bar 2 1.5 3.0 2 ---Flow rate (() mL/min), 15mm = 1.7cm2 filter area|Hold-up volume1: 100µL For water at 1bar 20 10 For methanol at 1bar 55 25 For air at 0.1bar 2 2 40 105 2 ---Flow rate (() mL/min), 25mm = 4.8cm2 filter area|Hold-up volume1: 200µL For water at 1bar 80 50 For methanol at 1bar 160 90 For air at 0.1bar 2 2 160 325 2 Water penetration point3 () Sterile filtration capability5 acc. No to the bacteria challenge test Non-pyrogenic according to the USP Yes No SRP 0.2µm K | S | Q | ACK With ethanol 1.1bar|16psi 3 2.0 30 3 55 800 3 60 1,800 4.0bar|58psi Yes SRP 0.45µm K | S | Q With ethanol 0.9bar|13psi 3 4.5 60 3 150 1,600 3 260 3,000 3.0bar|44psi No Filtration Devices Minisart® Syringe Filters 71 Minisart® Membrane Types Non-sterile packs: 50 (K), 200 (S), 500 (Q), 1000 (R)|sterile packs: individual packaged, 50 (ACK) Bubble point () NY 0.2µm K | Q | R | ACK With water 3.0bar|44psi NY 0.45µm K | Q | R | ACK With water 2.0bar|29psi NY Plus 0.2µm NY Plus 0.45µm PES 0.2µm K | Q K | Q K | Q | ACK With water 3.0bar|44psi With water 2.0bar|29psi With water 3.2bar|46psi PES -0.2µm K | Q With ethanol 0.95bar|14psi ---Flow rate (() mL/min), 4mm = 0.07cm2 filter area|Hold-up volume1: 10µL For water at 1bar For methanol at 1bar For air at 0.1bar 1.5 4 2 ---Flow rate (() mL/min), 15mm = 1.7cm2 filter area|Hold-up volume1: 100µL For water at 1bar 20 40 For methanol at 1bar 40 110 For air at 0.1bar 2 2 40 4 2 ---Flow rate (() mL/min), 25mm = 4.8cm2 filter area|Hold-up volume1: 200µL For water at 1bar 50 100 50 For methanol at 1bar 70 200 70 For air at 0.1bar 2 2 2 100 100 200 4 2 2 4 1,200 Water penetration point3 () 2.0bar|29psi Sterile filtration capability5 acc. Yes No Yes No Yes Yes to the bacteria challenge text Non-pyrogenic according to the USP 1 Hold-up volume after air purge 2 Hydrophilic membranes can filter dry air or gas but become impermeable to air or gas when wetted! 3 Hydrophobic membranes cannot be wetted with aqueous solutions unless you overcome their water penetration point or pre-wet them using an organic solvent (e.g. ethanol). 4 PES is suitable for solutions only containing up to 30% MeOH. 5 A ccording to the bacterial challenge test (BCT) with 1 × 107 cfu/cm2 Brevundimonas diminuta. Non-sterile RC Minisart® types are optimized for sample preparation and are not suitable for sterile filtration according to the bacteria challenge test. All other non-sterile Minisart® types with 0.2µm pore size can be sterilized by autoclaving or EO before use for sterile filtration. 6 For sterile packs ACK. Minisart® Standard Syringe Filters are not for medical use. 72 Filtration DevCicheasp teMr inisart® Syringe Filters Sample Preparation for Chromatography Ordering Information Minisart® RC (Regenerated Cellulose) in mm|EFA1 Membrane Housing 25mm RC PP 25mm RC PP 25mm RC PP 25mm RC PP 25mm RC PP 25mm RC PP 25mm RC PP 15mm RC PP 15mm RC PP 15mm RC PP 15mm RC PP 15mm RC PP 4mm RC PP 4mm RC PP 4mm RC PP 4mm RC PP Pore Size Connector Outlet 0.2µm Male Luer Slip 0.2µm Male Luer Slip 0.2µm Male Luer Slip 0.2µm Male Luer Slip 0.45µm Male Luer Slip 0.45µm Male Luer Slip 0.45µm Male Luer Slip 0.2µm Male Luer Slip 0.2µm Male Luer Slip 0.2µm Male Luer Slip 0.45µm Male Luer Slip 0.45µm Male Luer Slip 0.2µm Male Luer Slip 0.2µm Male Luer Slip 0.45µm Male Luer Slip 0.45µm Male Luer Slip Color|Printing White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed Blue Tray Blue Tray Yellow Tray Yellow Tray Sterile* Yes No No No No No No Yes No No No No No No No No Qty./Pkg. 50 50 200 500 50 200 500 50 50 500 50 500 50 500 50 500 Order No. 17764--------ACK 17764----------K 17764----------S 17764----------Q 17765----------K 17765----------S 17765----------Q 17761--------ACK 17761----------K 17761----------Q 17762----------K 17762----------Q 17821----------K 17821----------Q 17822----------K 17822----------Q Minisart® SRP (Hydrophobic PTFE) 25mm PTFE PP 25mm PTFE PP 25mm PTFE PP 25mm PTFE PP 25mm PTFE PP 25mm PTFE PP 25mm PTFE PP 25mm PTFE PP 15mm PTFE PP 15mm PTFE PP 15mm PTFE PP 15mm PTFE PP 15mm PTFE PP 15mm PTFE PP 15mm PTFE PP 15mm PTFE PP 15mm PTFE PP 4mm PTFE PP 4mm PTFE PP 4mm PTFE PP 0.2µm 0.2µm 0.2µm 0.2µm 0.2µm 0.45µm 0.45µm 0.45µm 0.2µm 0.2µm 0.2µm 0.2µm 0.2µm 0.45µm 0.45µm 0.45µm 0.45µm 0.2µm 0.45µm 0.45µm Male Luer Slip Male Luer Slip Male Luer Slip Male Luer Slip Hose Barb Male Luer Slip Male Luer Slip Male Luer Slip Male Spike Male Spike Male Luer Slip Male Luer Slip Male Luer Slip Male Spike Male Spike Male Luer Slip Male Luer Slip Male Luer Slip Male Luer Slip Male Luer Slip White, Printed Yes White, Printed No White, Printed No White, Printed No White, Printed No White, Printed No White, Printed No White, Printed No White, Printed No White, Printed No White, Printed Yes White, Printed No White, Printed No White, Printed No White, Printed No White, Printed No White, Printed No Blue Tray No Yellow Tray No Yellow Tray No 50 50 200 500 500 50 200 500 50 500 50 50 500 50 500 50 500 500 50 500 S7575------FXOSK 17575----------K 17575----------S 17575----------Q 1757A----------Q 17576----------K 17576----------S 17576----------Q 17558----------K 17558----------Q 17573--------ACK 17573----------K 17573----------Q 17559----------K 17559----------Q 17574----------K 17574----------Q 17844----------Q 17820----------K 17820----------Q Filtration Devices Minisart® Syringe Filters 73 Minisart® NY (Nylon) and NY25 Plus (Glass Fiber 0.7µm2 + Nylon) in mm|EFA1 Membrane Housing Pore Size Connector Outlet 25mm Nylon PP 0.2µm Male Luer Slip 25mm Nylon PP 0.2µm Male Luer Slip 25mm Nylon PP 0.45µm Male Luer Slip 25mm Nylon PP 0.45µm Male Luer Slip 15mm Nylon PP 0.2µm Male Luer Slip 15mm Nylon PP 0.2µm Male Luer Slip 15mm Nylon PP 0.45µm Male Luer Slip 15mm Nylon PP 0.45µm Male Luer Slip 25mm GF+Nylon PP 0.2µm Male Luer Slip 25mm GF+Nylon PP 0.2µm Male Luer Slip 25mm GF+Nylon PP 0.45µm Male Luer Slip 25mm GF+Nylon PP 0.45µm Male Luer Slip Color|Printing White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed White, Printed Sterile* Qty./Pkg. Order No. Yes 50 17845--------ACK No 500 17845----------Q Yes 50 17846--------ACK No 500 17846----------Q No 50 1776B----------K No 500 1776B----------Q No 50 1776C----------K No 500 1776C----------Q No 50 1784B----------K No 500 1784B----------Q No 50 1784C----------K No 500 1784C----------Q Minisart® PES (Polyethersulfone) Aqueous Filtration 15mm PES PP 0.22m 15mm PES PP 0.22m Male Luer Slip Male Luer Slip White White Yes 50 No 500 1776D--------ACK 1776D----------Q Minisart® PES- (Hydrophobic PES) Venting & Gas Filtration, Gamma Stable 25mm PES PP 0.2m Male Luer Slip 25mm PES PP 0.2m Male Luer Slip 25mm PES PP 0.2m Hose Barbs3 25mm PES PP 0.2m Hose Barbs3 White, Printed No White, Printed No White, Printed No White, Printed No * Sterile Minisart® syringe filters are individually packaged. If not stated otherwise, Minisart® units have been sterilized by ethylene oxide. Non-presterilized Minisart® units: RC, PTFE and nylon can be sterilized by autoclaving at 121°C for 30min. or by using ethylene oxide (EO). 1 Diameter of EFA Effective Filtration Area 2 0.7µm = GF particle retention pore size! 3 Hose barbs, inlet and outlet, stepped 4.46 mm diameter Minisart® Standard Syringe Filters are not for medical use. For technical product specifications, please see page 70. 50 500 50 500 1757H----------K 1757H----------Q 1757G----------K 1757G----------Q 74 0.1 0.45 0.22 Minisart® High Flow with PES 5.0 1.2 0.8 0.65 0.45 0.2 Minisart® NML with (SF)CA 0.2 Minisart® HY with PTFE Filtration Devices Minisart® Syringe Filters Minisart® NML Standard Syringe Filter Clarification and Sterilization by Filtration Filtration is the Optimal Method for Clarification and Sterilization of Liquids and Gases Sterilization by filtration is the fastest method for removal of bacterial cells from liquids, while minimizing the effects on ingredients. Minisart® NML with (surfactant-free) cellulose acetate (SF)CA is the best choice for all aqueous solutions with a pH of 4 to 8. It combines fast flow rates and is available in many d ifferent pore sizes also for the removal of larger particles. Minisart® High Flow with polyethersulfone (PES) is optimal for delivering the highest flow rates and for a broad pH compatibility range from 1 to 13. Due to the asymmetric membrane structure, the PES surface almost behaves like a prefilter. Both Minisart® types NML and High Flow are available pre-sterilized by ethylene oxide (EO) or gamma irradiation. Hydrophobic PTFE filters like Minisart® SRP are suitable for venting purposes and are additionally available in special formats with activated carbon. ---Minisart® Features Largest effective filtration area (EFA) of 6.2cm2 Low adsorption High flow rate ---High total throughput Low hold-up volume Gamma-irradiated or EO-sterilized 28mm EFA 33mm housing diameter (for NML and High Flow) 75 76 Filtration Devices Minisart® Syringe Filters Minisart® Standard Syringe Filters with MBS Housing Specifications Minisart® High Flow|NML|NML Plus with 28mm accessible membrane filtration area diameter, 150µL hold-up volume1 Minisart® HY|Acticosart with 26mm accessible membrane filtration area diameter, 150µL hold-up volume1 Minisart® Air with 15mm accessible membrane filtration area diameter, 100µm hold-up volume1 Housing material Methacrylate butadiene styrene (MBS) Membranes High Flow: PES = Polyethersulfone NML: (SF) CA = (Surfactant-free) Cellulose Acetate NML Plus: (SF) CA = (Surfactant-free) Cellulose Acetate HY|Acticosart|Air: Hydrophobic PTFE = Polytetrafluoroethylene Glass fiber prefilter NML Plus: Binder-free GF, 0.7µm particle retention Max. operating pressure High Flow: 6.0bar|87psi NML, NML Plus, HY, Air: 4.5bar|65psi Acticosart: 1bar|14.5psi Housing burst pressure 7bar|102psi (not determined for Acticosart) Max. temperature 60°C Sterilization Non-sterile Minisart® High Flow, NML and NML Plus can be or sterilized by ethylene oxide (EO) or by gamma irradiation. Non-sterile Minisart® HY, Acticosart, Air* can be sterilized by ethylene oxide (EO). Minisart® Membrane Types PES 0.1µm PES 0.2µm PES SFCA 0.45µm 0.2µm SFCA CA CA 0.45µm 0.65µm 0.8µm CA 1.2µm CA 5.0µm Non-sterile packages: K 500 (Q, HYQ), 1000 (R), sterile packs: individually packaged: 50 (K, GUK, HYK, HNK) K | K | K | K | K GUK | GUK | GUK | GUK | Q Q Q Q K | GUK | Q K | Q K | Q Bubble point () With water With water With water With water With water With water With water With water With water 5.0bar| 3.2bar| 2.0bar| 3.2bar| 2.0bar| 1.3bar| 0.8bar| 0.7bar| 0.4bar| 73psi 46psi 29psi 46psi 29psi 19psi 12psi 10psi 6psi Flow Rate for2|3 (mL/min) 28mm for water at 1bar 15mm for air at 0.1bar 26mm for air at 0.1bar 40 140 220 60 160 250 400 500 600 Water penetration point3 () Sterile filtration capability4 Yes Yes No Yes No No No No No acc. to the bacteria challenge test Non-pyrogenic according to the USP Yes5 Yes5 Yes5 Yes5 Yes5 Yes5 Yes5 Yes5 Yes5 Filtration Devices Minisart® Syringe Filters 77 Minisart® Membrane Types GF+SFCA GF+SFCA GF+CA 0.2µm 0.45µm 1.2µm GF 0.7µm PTFE 0.2µm PTFE 1.0µm Acticosart PTFE (Air) 0.2µm Non-sterile packages: K | Q K | Q Q 500 (Q, HYQ), 1000 (R), sterile packs: individually packaged, 50 (K, GUK, HYK, HNK) K | Q K | Q HYQ Q Q | HNK Bubble point () With water With water With water With water With ethanol With ethanol With ethanol With ethanol 3.2bar| 2.0bar| 0.7bar| 0.5bar| 1.4bar| 0.5bar| 0.9bar| 1.0bar| 46psi 29psi 10psi 7psi 20psi 7psi 13psi 14psi Flow rate for 2|3 (mL/min) 28mm for water at 1bar 15mm for air at 0.1bar 26mm for air at 0.1bar 60 160 350 450 800 2,000 4,000 2,300 Water penetration point3 () 4.0bar| 1.5bar| 58psi 22psi Sterile filtration capability4 Yes No No No Yes No according to the bacteria challenge test Non-pyrogenic according Yes5 to the USP 1 Hold-up volume after air purge 2 Hydrophilic membranes can filter dry air or gas but become impermeable to air or gas when wetted! 3 Hydrophobic membranes cannot be wetted with aqueous solutions unless you overcome their water penetration point. 4 A ccording to bacterial challenge test (BCT) with 1×107 cfu/cm2 Brevundimonas diminuta. All non-sterile Minisart® types listed above can be sterilized according to the method recommended in this table. 5 For sterile packs K | GUK *Minisart® Air can be sterilized by Gamma irradiation according to the following parameters: Range 25 40 kGy (validated with 50 kGy). Standard Minisart® Syringe Filters are not for medical use. N.a. N.a. 3.2bar| 44psi Yes 78 Preparation of Aqueous Liquids Filtration Devices Minisart® Syringe Filters Ordering Information Minisart® High Flow (PES Polyethersulfone) in mm|EFA1 Membrane Housing 28mm PES MBS 28mm PES MBS 28mm PES MBS 28mm PES MBS 28mm PES MBS 28mm PES MBS 28mm PES MBS 28mm PES MBS 28mm PES MBS 28mm PES MBS 28mm PES MBS Pore Size Connector Outlet 0.1µm Male Luer Lock 0.22µm Male Luer Lock 0.22µm Male Luer Lock 0.22µm Male Luer Slip 0.22µm Male Luer Lock 0.22µm Male Luer Slip 0.45µm Male Luer Lock 0.45µm Male Luer Lock 0.45µm Male Luer Slip 0.45µm Male Luer Slip 0.45µm Male Luer Slip Color|Printing Dark Red Royal Blue Royal Blue Royal Blue Royal Blue Royal Blue Amber Amber Amber Amber Amber Sterile* Yes Yes# Yes Yes No No Yes No Yes# Yes No Qty./Pkg. Order No. 50 16553----------K 50 16532--------GUK 50 16532----------K 50 16541----------K 500 16532----------Q 500 16541----------Q 50 16537----------K 500 16537----------Q 50 16533--------GUK 50 16533----------K 500 16533----------Q Minisart® NML ((SF)CA (Surfactant-free) Cellulose Acetate) 28mm SFCA MBS 0.2µm Male Luer Lock 28mm SFCA MBS 0.2µm Male Luer Lock 28mm SFCA MBS 0.2µm Male Luer Lock 28mm SFCA MBS 0.2µm Male Luer Slip 28mm SFCA MBS 0.2µm Male Luer Slip 28mm SFCA MBS 0.45µm Male Luer Lock 28mm SFCA MBS 0.45µm Male Luer Lock 28mm SFCA MBS 0.45µm Male Luer Lock 28mm SFCA MBS 0.45µm Male Luer Slip 28mm SFCA MBS 0.45µm Male Luer Slip 28mm CA MBS 0.65µm Male Luer Slip 28mm CA MBS 0.8µm Male Luer Lock 28mm CA MBS 0.8µm Male Luer Lock 28mm CA MBS 0.8µm Male Luer Lock 28mm CA MBS 1.2µm Male Luer Lock 28mm CA MBS 1.2µm Male Luer Lock 28mm CA MBS 5µm Male Luer Lock 28mm CA MBS 5µm Male Luer Lock Blue Blue Blue Blue Blue Yellow Yellow Yellow Yellow Yellow Pink Green Green Green Red Red Brown Brown Yes 50 Yes# 50 No 500 Yes 50 No 500 Yes 50 Yes# 50 No 500 Yes 50 No 500 Yes 50 Yes 50 Yes# 50 No 500 Yes 50 No 500 Yes 50 No 500 S6534------FMOSK S6534------FMGUK S6534------FM--Q S7597------FXOSK S7597------FX--Q S6555------FMOSK S6555------FMGUK S6555------FM--Q S7598------FXOSK S7598------FX--Q 16569----------K 16592----------K 16592--------GUK 16592----------Q 17593----------K 17593----------Q S7594------FMOSK 17594----------Q Filtration Devices Minisart® Syringe Filters 79 Minisart® NML Plus (Glass Fiber 0.7µm2 + SFCA) in mm|EFA1 Membrane Housing Pore Size 28mm GF+SFCA MBS 0.2µm 28mm GF+SFCA MBS 0.2µm 28mm GF+SFCA MBS 0.45µm 28mm GF+SFCA MBS 0.45µm 28mm GF+CA MBS 1.2µm 28mm GF MBS 0.7µm2 28mm GF MBS 0.7µm2 Connector Outlet Male Luer Lock Male Luer Lock Male Luer Lock Male Luer Lock Male Luer Lock Male Luer Lock Male Luer Lock Color|Printing Blue Blue Yellow Yellow Red White White Sterile* Yes No Yes No No No No Qty./Pkg. Order No. 50 17823----------K 500 17823----------Q 50 17829----------K 500 17829----------Q 500 17825----------Q 50 17824----------K 500 17824----------Q Minisart® HY (hydrophobic PTFE), for Venting and Gas Filtration 26mm PTFE MBS 0.2µm Male Luer Lock 26mm PTFE MBS 1µm Male Luer Lock 26mm PTFE MBS 0.2µm Male Luer Lock Clear Clear Clear Yes 50 No 500 No 500 S6596------FMOSK 1659A--------HYQ S6596------FM--Q Minisart® High Flow (PES Polyethersulfone) Aqueous Filtration 28mm PES MBS 0.1µm Male Luer Lock Dark Red Yes 50 16553----------K Minisart® Air (Hydrophobic PTFE) Venting 15mm PTFE MBS 15mm PTFE MBS 0.2µm 0.2µm Male Luer Slip Yellow Male Luer Slip + Needle Yellow No 500 1751A----------Q No 500 1751A----------Q Minisart® Acticosart with Dome Reservoir + Hydrophobic PTFE Venting & Ultracleaning of Gases 26mm Active carbon MBS 0.45µm Male Luer Slip Blue No 500 17840----------Q * Sterilized Minisart® units are individually packaged. If not stated otherwise, Minisart® are sterilized by ethylene oxide. #-mark indicates sterilization by gamma irradiation. Non-presterilized Minisart® units: High Flow, NML, NML Plus and HY can be sterilized by ethylene oxide; High Flow, NML and NML Plus can also be sterilized by gamma irradiation 1 Diameter of EFA Effective Filtration Area 2 0.7µm = GF particle retention pore size! Minisart® Standard Syringe Filters are not for medical use. For technical product specifications, please see page 76. 80 5.0 0.45 0.2 0.2 Filtration Devices Minisart® Syringe Filters CE-Minisart® Syringe Filters For medical use Sterile Filtration and Sterile Venting The medical device CE-Minisart® NML and Ophthalsart with (surfactant-free) cellulose acetate ((SF)CA), and CE-Minisart® HY and SRP with hydrophobic PTFE are frequently used for sterile filtration and | or clarification of aqueous and oily liquids and other medical applications. CE-Minisart® NML with a 5 µm cellulose acetate (CA) membrane removes particulates or coagulates offering high total throughput under sterile conditions. Hydrophobic PTFE filters are suitable for venting purposes. All CE-Minisart® filters are intended to be used in a laboratory environment before use for patient care. ---Minisart® Features Low adsorption Gamma-irradiated or EO-sterilized Biocompatible acc. to ISO 10993-1 Filtration Devices Minisart® Syringe Filters 81 CE-Minisart® Syringe Filters Specifications CE-Minisart® NML / Ophthalsart with 28mm accessible membrane filtration diameter, 150µL hold-up volume1 CE-Minisart® HY with 26mm accessible membrane filtration diameter, 200µL hold-up volume1 CE-Minisart® SRP with 25mm accessible membrane filtration diameter, 200µL hold-up volume1 Housing material NML/Ophthalsart/HY: Methacrylate butadiene styrene (MBS) SRP: Polypropylene (PP) Membranes NML /Ophthalsart: (SF)CA = (Surfactant-free) Cellulose Acetate NML (5µm): CA = Cellulose Acetate HY: Hydrophobic PTFE = Polytetrafluoroethylene SRP: Hydrophobic PTFE = Polytetrafluoroethylene Max. operating pressure 4.5bar|65psi Housing burst pressure 7bar|102psi Max. temperature 60°C Sterilization Non-sterile Minisart® NML can be sterilized by ethylene oxide (EO) or gamma irradiation. Non-sterile Minisart® HY can be sterilized by ethylene oxide (EO) Minisart® type with regards to membrane SFCA 0.2µm Non-sterile packages: 500 (Q, HYQ), sterile packs: individually packaged: 50 (K, GUK, HYK, ACK) K | GUK | Q Bubble point () With water 3.2bar| 46psi SFCA 0.45µm K | GUK | Q With water 2.0bar| 29psi CA 5.0µm K With water 0.4bar| 6psi PTFE 0.2µm HYK | HYQ With ethanol 1.4bar| 20psi PTFE 0.2µm ACK With ethanol 1.1bar| 16psi Flow Rate for2 (mL/min) 28mm for water at 1bar 60 25mm with ethanol at 1 bar 25mm for air at 0.1bar 26mm for air at 0.1bar 160 600 2,000 Water penetration point2 () 4.0bar| 58psi Sterile filtration capability3 Yes No No Yes acc. to the bacteria challenge test Non-pyrogenic Yes Yes Yes Yes Biocompatible acc. to ISO 10993-1 1 Hold-up volume after air purge 2 Hydrophobic membranes cannot be wetted with aqueous solutions unless you overcome their water penetration point. 3 A ccording to the bacterial challenge test (BCT) with 1×107 cfu/cm2 Brevundimonas diminuta. All non-sterile Minisart® types listed above can be sterilized according to the sterilization recommendation in this table. 60 1,800 4.0bar| 58psi Yes Yes 82 Filtration Devices Minisart® Syringe Filters CE-Minisart® Syringe Filters for Medical Use Ordering Information Minisart® NML ((SF)CA (Surfactant-free) Cellulose Acetate) Aqueous Filtration in mm|EFA1 Membrane Housing Pore Size Connector Outlet 28mm SFCA MBS 0.2µm Male Luer Lock 28mm SFCA MBS 0.2µm Male Luer Lock 28mm SFCA MBS 0.2µm Male Luer Lock 28mm SFCA MBS 0.2µm Male Luer Slip 28mm SFCA MBS 0.2µm Male Luer Slip 28mm SFCA MBS 0.45µm Male Luer Lock 28mm SFCA MBS 0.45µm Male Luer Lock 28mm SFCA MBS 0.45µm Male Luer Lock 28mm SFCA MBS 0.45µm Male Luer Slip 28mm SFCA MBS 0.45µm Male Luer Slip 28mm CA MBS 5µm Male Luer Lock Color|Printing Blue Blue Blue Blue Blue Yellow Yellow Yellow Yellow Yellow Brown Sterile* Yes Yes# No Yes No Yes Yes# No Yes No Yes Qty./Pkg. Order No. 50 16534----------K* 50 16534--------GUK* 500 16534----------Q* 50 17597----------K * 500 17597----------Q* 50 16555----------K* 50 16555--------GUK* 500 16555----------Q* 50 17598----------K* 500 17598----------Q* 50 17594----------K* Minisart® Ophthalsart (SFCA Cellulose Acetate) Aqueous Filtration 28mm SCFA MBS 0.2µm Male Luer Slip Pink Yes 50 17528----------K* Minisart® HY (Hydrophobic PTFE), for Venting and Gas Filtration 26mm PTFE MBS 0.2µm Male Luer Lock 26mm PTFE MBS 0.2µm Male Luer Lock 26mm PTFE MBS 0.2µm Male Luer Locka Clear Clear Clear Yes 50 No 500 No 500 16596--------HYK* 16596--------HYQ* 16599--------HYQ* Minisart® SRP (Hydrophobic PTFE) Venting & Gas Filtration 25mm PTFE PP 0.2µm Male Luer Slip White, Printed Yes 50 * Article numbers are only available in: EU/EEA and in registered countries. ** Sterilized Minisart® units are individually packaged. If not stated otherwise, Minisarts are sterilized by ethylene oxide. #-mark indicates sterilization by gamma irradiation Non-presterilized Minisart® units: (SF)CA can be sterilized by ethylene oxide or gamma irradiation. PTFE can be sterilized by ethylene oxide. a Connector inlet: Male Luer Slip (all other Minisart® have Female Luer Lock inlet(s)). 1 Diameter of EFA Effective Filtration Area For technical product specifications, please see page 81. 17575--------ACK*Adobe InDesign 15.0 (Windows) Adobe PDF Library 15.0