សៀវភៅណែនាំអ្នកប្រើប្រាស់ សំណួរ និងចម្លើយចម្លើយ
ខែសីហា 23, 2025 - 07:50 PM
នេះបើតាម Cytek ២២៨៣៥- ពណ៌ បន្ទះ AML instructions, if you need to store the samples for more than 2 hours before collecting data, you should follow the steps for cell fixation in tubes using 1% paraformaldehyde. Here's a summary of the steps:
1. Dilute 4% paraformaldehyde in PBS to make a 1% paraformaldehyde solution.
2. Add 300 μL of the 1% paraformaldehyde solution to the cell pellet.
3. Vortex thoroughly.
4. Incubate for 20 minutes at room temperature, protected from light.
5. Add 3 mL of Stain Buffer.
6. Centrifuge at 400 x g for 5 minutes at room temperature.
7. Decant and blot on a paper towel.
8. Vortex thoroughly.
9. Resuspend in 300 μL Stain Buffer for Single Stain Reference Controls and 400 μL for Multicolor Samples ។
10. Store at 4°C and acquire within 24 hours post-fixation.
By following these steps, you can ensure the stability and integrity of your samples if you need to store them for an extended period before data acquisition.
បន្ថែមមតិយោបល់ថ្មី។