This comprehensive user manual provides detailed laboratory instructions for operating the Olink® Explore 384 system. It guides researchers through the entire workflow for protein biomarker discovery, from sample preparation and dilution to incubation, amplification (PCR1 and PCR2), pooling, library purification, and quality control.

The manual covers protocols utilizing various automation platforms, including Formulatrix F.A.S.T.™, Hamilton Microlab® STAR, SPT Labtech Mosquito, and Eppendorf® epMotion, ensuring flexibility in laboratory setups. It details reagent requirements, step-by-step procedures, and important notes for optimal results and troubleshooting.

For further information and resources, Olink provides access to associated documentation, including overview manuals and sequencing guides, available at olink.com/downloads. Technical support can be reached at support@olink.com.

The Olink® Explore platform is based on Olink's proprietary PEA™ technology, designed for multiplex immunoassay applications in human protein biomarker discovery for research use only.

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Olink® Explore 384 User Manual

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