Olink® Explore User Manual: Protein Biomarker Discovery with PEA and NGS

Introduction to the Olink® Explore Platform

The Olink® Explore platform represents a significant advancement in high-multiplex, high-throughput protein biomarker discovery. This sophisticated system leverages Proximity Extension Assay (PEA) technology, coupled with an innovative Next Generation Sequencing (NGS) readout, to measure the relative concentrations of proteins in liquid biopsies. Designed for researchers in drug development, clinical research, and basic life science, Olink® Explore facilitates large-scale discovery studies, particularly focusing on the low-abundant plasma proteome.

This comprehensive user manual provides detailed instructions for operating the Olink® Explore system, including its various reagent kit combinations such as the Olink® Explore 1536, Olink® Explore 4 x 384, and Olink® Explore 384 single panel kits. It covers essential aspects from laboratory setup and safety protocols to detailed workflow procedures, quality control measures, and instrument operation.

For further information and resources, users are encouraged to consult the Olink® website, including documentation for data processing and analysis via the Olink® MyData Cloud User Guide, and specific instrument manuals from manufacturers like Illumina, Agilent, Eppendorf, and Thermo Fisher Scientific.

Technology: Proximity Extension Assay (PEA) with Next Generation Sequencing (NGS) readout.
Application: High-multiplex protein biomarker discovery in liquid biopsies.
Target Audience: Researchers in drug development, clinical research, and life sciences.
Key Features: Semi-automated protocol, comprehensive data analysis tools.

For technical support, please contact Olink Proteomics at support@olink.com.

	Olink® Explore User Manual: Protein Biomarker Discovery Platform Guide Comprehensive user manual for the Olink® Explore platform, detailing its high-multiplex, high-throughput protein biomarker discovery capabilities using Proximity Extension Assay (PEA) and Next Generation Sequencing (NGS) technology. Includes step-by-step instructions, equipment requirements, and guidelines for researchers.
	Olink® Explore 3072 User Manual: Protein Biomarker Discovery Workflow This user manual provides detailed instructions for the Olink® Explore 3072 system, a multiplex immunoassay platform designed for protein biomarker discovery. It guides researchers and laboratory staff through the entire workflow, from sample preparation and amplification to quality control and sequencing, leveraging Olink's proprietary PEA™ technology.
	Olink® Focus User Manual: Protein Biomarker Validation and Analysis User manual for Olink® Focus multiplex immunoassay panels. Learn about Proximity Extension Assay (PEA) technology, sample preparation, experimental design, operation, quality control, and data analysis for protein biomarker validation. Includes details on reagents, consumables, equipment, and alternative system configurations.
	Olink Explore 384 User Manual: Laboratory Protocol Guide Comprehensive user manual for the Olink Explore 384 system, detailing laboratory protocols for protein biomarker discovery. Covers sample preparation, incubation, PCR amplification, library purification, and quality control using various automation platforms.
	Olink Explore HT User Manual: A Guide to Protein Biomarker Discovery This user manual provides comprehensive instructions for the Olink Explore HT platform, a high-multiplex immunoassay system for protein biomarker discovery using PEA technology and NGS. It covers reagent kits, laboratory protocols, equipment, and quality control for advanced proteomic research.
	Olink® Explore Sequencing using NextSeq™ 2000 User Manual User manual providing detailed instructions for sequencing Olink® Explore Libraries using the Illumina® NextSeq™ 2000 instrument. Covers setup, reagent preparation, run configuration, and monitoring for human protein biomarker discovery.
	Olink® Target 96 User Manual This user manual provides comprehensive instructions for operating the Olink® Target 96 system, detailing the Proximity Extension Assay (PEA) technology, experimental setup, sample preparation, operation procedures, data analysis, and troubleshooting.
	Olink Target 48 Short Instructions Concise instructions for performing the Olink® Target 48 assay, covering incubation, extension, and detection steps for protein expression analysis.

Introduction to the Olink® Explore Platform

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