AmScope B120C-DK-E1 Binocular LED Darkfield Microscope User Manual

מָבוֹא

This manual provides instructions for the proper setup, operation, and maintenance of your AmScope B120C-DK-E1 Binocular LED Darkfield Microscope. This instrument is designed for educational, clinical, and laboratory applications, offering both brightfield and darkfield observation capabilities. Please read this manual thoroughly before using the microscope to ensure safe and effective operation.

מידע בטיחות

• Always handle the microscope with care. Avoid sudden movements or impacts.
• Ensure the microscope is placed on a stable, level surface to prevent tipping.
• נתק את כבל החשמל לפני ניקוי או ביצוע כל תחזוקה.
• Do not expose the microscope to direct sunlight, high temperatures, or high humidity.
• השתמש רק במתאם החשמל שצוין.
• Keep the optical components clean. Avoid touching lenses with bare hands.
• Do not attempt to disassemble or repair the microscope yourself. Contact qualified service personnel.

רכיבים ותכונות

Familiarize yourself with the main components of your AmScope B120C-DK-E1 microscope:

איור 1: מֵעַלview of the AmScope B120C-DK-E1 Binocular Compound Microscope, highlighting key features such as the 2-layer mechanical stage, 360-degree adjustable head, 40X-2500X magnification, coaxial coarse and fine focus, and LED sub-stagתאורה אלקטרונית.

איור 2: The Siedentopf binocular head, featuring 360-degree rotation and a 30-degree inclination for comfortable viewing and posture adjustment.

איור 3: מְפוֹרָט view of the 2-layer mechanical stage and the coaxial coarse and fine focus knobs, designed for precise specimen movement and focusing.

איור 4: The LED sub-stage lighting system, providing cool, energy-efficient, and daylight-balanced illumination for improved contrast and resolution.

מרכיבי מפתח:

• Siedentopf Binocular Head: Rotates 360 degrees with a 30-degree inclination for ergonomic viewing.
• עיניות: Includes 10X and 25X widefield eyepieces for various magnification levels.
• עדשות אובייקטיביות: Achromatic, color-coded objective lenses (e.g., 4X, 10X, 40X, 100X oil) mounted on a revolving nosepiece.
• מכני S.tage: A 2-layer stage with low-position controls for precise X-Y movement of slides.
• Coaxial Focus Knobs: Separate coarse and fine focus adjustments for accurate focusing.
• תאורת LED: תת-סtage LED light source with adjustable brightness.
• מַעֲבֶה: Includes a brightfield condenser and a darkfield condenser for specialized observation.
• 1MP Digital Eyepiece Camera: For capturing images and videos, compatible with Windows, Mac, and Linux.

הגדרה

1. פריקת אריזה: הוצא בזהירות את כל הרכיבים מהאריזה. שמור את האריזה להובלה או אחסון עתידיים.
2. מיקום: Place the microscope on a stable, vibration-free surface away from direct sunlight and heat sources.
3. התקנת ראש: Loosen the head retaining screw on the top of the microscope body. Gently insert the binocular head into the opening, ensuring it is fully seated. Tighten the retaining screw.
4. התקנת עינית: Remove the protective caps from the eyepiece tubes. Insert the desired eyepieces (10X or 25X) into the tubes.
5. Objective Lens Installation: Rotate the revolving nosepiece to ensure there is no objective lens directly above the stage. Screw the objective lenses into the nosepiece in increasing order of magnification (e.g., 4X, 10X, 40X, 100X).
6. חיבור חשמל: Connect the power cord to the microscope's power input and then to a suitable electrical outlet.
7. Digital Eyepiece Camera Installation: If using the digital eyepiece, remove one of the standard eyepieces and insert the 1MP digital eyepiece camera into the eyepiece tube. Connect the camera to your computer via USB.

הוראות הפעלה

1. Brightfield Observation

איור 5: Examples of magnification levels (10X, 100X, 400X, 1000X) achievable with the AmScope B120C-DK-E1, utilizing various objective lenses and eyepieces, including the additional 25X eyepieces for up to 2500X magnification.

1. הפעלה: Turn on the main power switch. Adjust the LED illuminator brightness using the dimmer control.
2. Stage התאמה: הורד את ה-s המכאניtage using the coarse focus knob to create enough space to place a slide.
3. Slide Placement: Place a prepared specimen slide onto the mechanical stagה, מאבטח אותו עם ה-stage clips. Use the X-Y stage controls to position the specimen over the light aperture.
4. בחירת הגדלה: Rotate the revolving nosepiece to select the lowest power objective lens (e.g., 4X). Ensure it clicks into place.
5. התמקדות:
   • בזמן שאתה מסתכל דרך העיניות, הרם באיטיות את ה-stage using the coarse focus knob until the specimen comes into rough focus.
   • Use the fine focus knob to achieve sharp focus.
   • Adjust the interpupillary distance (distance between eyepieces) and diopter adjustment on the eyepiece for comfortable viewing and to correct for differences in vision between your eyes.
6. Increasing Magnification: Once focused at low power, rotate the nosepiece to a higher power objective (e.g., 10X, 40X). The microscope is parfocal, meaning only minor adjustments with the fine focus knob should be needed.
7. שימוש במטרת טבילה בשמן 100X:
   • After focusing with the 40X objective, rotate the nosepiece halfway between the 40X and 100X objectives.
   • הניחו טיפה קטנה של שמן טבילה ישירות על מרכז שקופית הדגימה.
   • Rotate the 100X objective into the oil drop. The objective lens should make contact with the oil.
   • Use only the fine focus knob to bring the image into sharp focus. Do not use the coarse focus knob with the 100X objective.
   • After use, clean the 100X objective lens and the slide immediately with lens cleaning solution and lens paper.
8. כוונון מעבה: Adjust the iris diaphragm on the condenser to control the amount of light and contrast. For higher magnifications, open the diaphragm more. For lower magnifications, close it slightly. The condenser can also be centered for optimal illumination.

2. Darkfield Observation

The AmScope B120C-DK-E1 includes a darkfield condenser for observing unstained, live specimens.

1. Condenser Exchange: Remove the brightfield condenser from its mount beneath the stage. Carefully insert the darkfield condenser into the mount. Ensure it is seated correctly.
2. הכנת דגימה: Darkfield observation works best with thin, unstained specimens.
3. תְאוּרָה: Turn on the LED illuminator and set it to a high brightness level.
4. התמקדות: Use a low power objective (e.g., 10X or 20X, if available) first. Focus on the specimen as you would in brightfield. The specimen will appear brightly lit against a dark background.
5. Centering the Darkfield Condenser: Some darkfield condensers require centering. If your condenser has centering screws, adjust them until the darkfield effect is uniform across the field of view.
6. הגדלה גבוהה יותר: For higher magnifications, you may need to use a specialized darkfield objective or ensure your standard objectives are compatible. Always ensure sufficient immersion oil is used with 100X darkfield objectives.

3. Digital Eyepiece Camera Operation

The included 1MP digital eyepiece camera allows for image capture and live viewעל מחשב.

איור 6: The AmScope B120C-DK-E1 microscope connected to a laptop, demonstrating live digital viewing and image capture capabilities via the 1MP digital eyepiece camera.

1. התקנת תוכנה: Install the professional microscopy software provided (or download the latest version from the AmScope website) onto your Windows, Mac, or Linux computer.
2. חיבור מצלמה: Insert the digital eyepiece camera into one of the microscope's eyepiece tubes and connect it to your computer via the USB cable.
3. הפעלת תוכנת: Open the installed microscopy software. The software should detect the camera automatically.
4. לִחיוֹת View: The software will display a live feed from the microscope. Adjust microscope focus and illumination for the best image.
5. לכידת תמונה/וידאו: Use the software's functions to capture still images or record videos of your observations. The software also provides tools for image processing and measurement.

תַחזוּקָה

• ניקוי עדשות: Use a soft brush to remove dust from lens surfaces. For smudges or oil, use a small amount of lens cleaning solution on lens paper. Wipe gently in a circular motion. Never use abrasive cloths or harsh chemicals.
• ניקוי גוף: נגבו את גוף המיקרוסקופ בעזרת מטלית רכהamp מטלית. יש להימנע מלחות ברכיבים חשמליים.
• אִחסוּן: כאשר המיקרוסקופ אינו בשימוש, יש לכסות את המיקרוסקופ בכיסוי נגד אבק כדי להגן עליו מפני אבק ופסולת. יש לאחסן במקום קריר ויבש.
• שמן טבילה: Always clean immersion oil from the 100X objective and slides immediately after use. Dried oil can be difficult to remove and can damage the lens.

פתרון בעיות

מפרטים

אחריות ותמיכה

The AmScope B120C-DK-E1 microscope comes with a 5 שנות אחריות יצרן. For warranty claims, technical support, or service inquiries, please contact AmScope customer service directly.

For additional resources, software downloads, and frequently asked questions, please visit the official AmScope webאֲתַר:

www.amscope.com