1. הקדמה
The AmScope D120A-MS is a dual-view compound monocular microscope designed for high magnification observation in biological and educational applications. It features a versatile viewing head, multiple objective lenses, Brightfield illumination, and a mechanical stage for precise specimen manipulation. This manual provides essential information for the proper setup, operation, and maintenance of your microscope.
2. הנחיות בטיחות
- Always handle the microscope with care. Avoid sudden movements or impacts.
- Ensure the microscope is placed on a stable, level surface to prevent tipping.
- Connect the power cord to a grounded electrical outlet of the correct voltage (110V).
- Do not touch optical surfaces with bare hands. Use lens paper or a soft, lint-free cloth for cleaning.
- Unplug the microscope from the power source before cleaning or performing any maintenance.
- הרחק את המיקרוסקופ מאור שמש ישיר, טמפרטורות גבוהות, אבק וכימיקלים קורוזיביים.
- Supervise children or inexperienced users to ensure safe operation.
3. תכולת החבילה
פרקו בזהירות את כל הרכיבים וודאו שהפריטים הבאים כלולים:
- AmScope D120A-MS microscope with mechanical stage
- WF10x eyepiece
- WF16x eyepiece
- 4x DIN achromatic objective
- 10x DIN achromatic objective
- 40xS DIN achromatic objective (spring-loaded)
- 100xS (oil) DIN achromatic objective (spring-loaded)
- (2) Color filters
- נורה חילוף
- מכסה אבק
- מדריך הוראות (מסמך זה)
4. זיהוי רכיבים
Familiarize yourself with the main parts of your AmScope D120A-MS microscope:
איור 4.1: מֵעַלview of the AmScope D120A-MS microscope. This image displays the complete microscope assembly, including the base, stage, objective lenses, and dual-view monocular head.
- עינית (עינית): לאן אתה מסתכל view the specimen. The D120A-MS includes WF10x and WF16x eyepieces.
- מונוקולרי Viewראש ינג: The upper part of the microscope containing the eyepiece tube. This model features a dual-view head with a fixed 45-degree inclined tube and a vertical port.
- חתיכת אף מסתובבת: Holds the objective lenses and allows for easy switching between magnifications.
- עדשות אובייקטיביות: The primary magnification components (4x, 10x, 40xS, 100xS oil).
- מכני S.tage: A platform where the specimen slide is placed, with controls for precise X-Y movement.
- Stage קליפים: אבטח את שקופית הדגימה על המנגנון המכניtage.
- Coarse Focus Knob: Large knob for rapid focusing adjustments.
- Fine Focus Knob: Small knob for precise focusing adjustments.
- Illuminator (Light Source): ממוקם מתחת ל-stage, provides light for viewing. This model uses variable-intensity tungsten illumination.
- קונדנסר אבי: Located below the stage, focuses light onto the specimen. Features a 1.25 NA and an iris diaphragm.
- איריס דיאפרגמה: Controls the amount of light passing through the specimen.
- בָּסִיס: The stable bottom support of the microscope.
5. הרכבה והתקנה
Follow these steps to set up your microscope for initial use:
- פריקת אריזה: Carefully remove the microscope and all accessories from the packaging. Retain the original packaging for future transport or storage.
- מיקום: Place the microscope on a sturdy, level surface, away from vibrations and direct sunlight.
- התקנת עינית: Insert the desired eyepiece (WF10x or WF16x) into the inclined eyepiece tube of the monocular head. Gently twist to secure it.
- התקנה מטרתית: The objective lenses are typically pre-installed. If not, carefully screw each objective into the revolving nosepiece in increasing order of magnification (4x, 10x, 40xS, 100xS). Ensure they are finger-tight.
- חיבור חשמל: Connect the power cord to the microscope's power input and then to a standard 110V electrical outlet.
- כיסוי אבק: כאשר אינו בשימוש, כסו את המיקרוסקופ בכיסוי האבק המצורף כדי להגן עליו מפני אבק ופסולת.
איור 5.1: תקריב view of the objective lenses mounted on the revolving nosepiece. This image shows the 4x, 10x, 40xS, and 100xS (oil) objectives in position.
6. פעולה בסיסית
This section outlines the fundamental steps for observing specimens with your microscope.
- הפעלה: Flip the power switch located on the base of the microscope. The illuminator should light up.
- התאם את התאורה: Use the variable intensity control knob to set the desired brightness. Adjust the iris diaphragm on the Abbe condenser to optimize contrast and resolution for your specimen.
- דגימת מקום: Rotate the revolving nosepiece to select the lowest power objective (4x). Lower the mechanical stage using the coarse focus knob. Place a prepared specimen slide onto the mechanical stage ואבטח אותו באמצעות ה-stage קליפים.
- Position Specimen: השתמש ב-XY stage control knobs to move the slide until the area of interest is centered under the objective lens.
- התמקדות:
- While looking through the eyepiece, slowly raise the stage באמצעות coarse focus knob until the specimen comes into rough focus.
- השתמש ב- fine focus knob to achieve a sharp, clear image.
- שנה הגדלה: Once focused at low power, rotate the revolving nosepiece to switch to a higher power objective (e.g., 10x, 40xS). Due to the parfocal design, only minor adjustments with the fine focus knob should be needed to bring the image back into sharp focus.
- Using the 100xS Oil Immersion Objective:
- After focusing with the 40xS objective, rotate the nosepiece halfway between the 40xS and 100xS objectives.
- Place a small drop of immersion oil directly onto the center of the specimen slide where the light passes through.
- Carefully rotate the 100xS objective into the oil drop until it clicks into place. The objective should be immersed in the oil.
- השתמש רק ב- fine focus knob to bring the image into sharp focus. Do not use the coarse focus knob with the 100xS objective.
- After use, clean the 100xS objective and the slide immediately with lens paper moistened with lens cleaning solution to remove all immersion oil.
- כָּפוּל-View פונקציונליות: The vertical port on the monocular head can accept a second eyepiece for simultaneous viewing by another person or a camera mount for digital image capture (eyepiece and camera sold separately). Dioptric adjustment on the upright tube accommodates individual eye-strength differences.
איור 6.1: The AmScope D120A-MS microscope demonstrating its digital image capture capability by connecting to a laptop. This setup utilizes the vertical port for a camera attachment.
7. תחזוקה וטיפול
Proper maintenance ensures the longevity and optimal performance of your microscope.
- ניקוי עדשות: Use only specialized lens paper and lens cleaning solution for optical surfaces. Gently wipe in a circular motion. Never use abrasive cloths or harsh chemicals.
- ניקיון כללי: נגבו את גוף המיקרוסקופ בעזרת מטלית רכהamp מטלית. יש להימנע מלחות ברכיבים חשמליים.
- אִחסוּן: Always cover the microscope with the dust cover when not in use. Store it in a dry, dust-free environment.
- החלפת נורה: If the illuminator bulb burns out, unplug the microscope. Refer to the base of the microscope for access to the bulb compartment. Carefully replace the old bulb with the provided spare bulb, ensuring not to touch the glass part of the new bulb with bare hands.
- חלקים מכניים: Do not attempt to lubricate or disassemble any mechanical parts. If issues arise, contact customer support.
8. פתרון תקלות
סעיף זה עוסק בבעיות נפוצות שאתה עשוי להיתקל בהן.
| בְּעָיָה | סיבה אפשרית | פִּתָרוֹן |
|---|---|---|
| אין תאורה | Power cord disconnected, power switch off, bulb burnt out | Check power connection, turn on switch, replace bulb |
| התמונה מטושטשת או לא בפוקוס | Incorrect focus adjustment, objective not fully engaged, dirty lens | Adjust coarse/fine focus, rotate nosepiece until objective clicks, clean lenses |
| Dark field of view | Iris diaphragm closed, illuminator too dim, condenser too low | Open iris diaphragm, increase illuminator brightness, adjust condenser height |
| אבק או כתמים בפנים view | Dust on eyepiece, objective, or slide | Clean eyepiece, objective, or slide with lens paper |
| Cannot focus at 100x | No immersion oil, air bubbles in oil, coarse focus used | Apply immersion oil, remove air bubbles, use only fine focus |
9. מפרט טכני
Detailed specifications for the AmScope D120A-MS microscope:
- רֹאשׁ: Compound dual-view monocular, 45-degree inclined, 360-degree rotatable
- עיניות: WF10x, WF16x (with pointer)
- מטרות: 4X, 10x, 40xS (spring), 100xS (oil, spring) DIN achromatic
- טווח הגדלה: פי 40 - פי 1600
- Stage: ס' מכאניtage with X-Y axis movement, stagעצור
- תצורת תאורה: Transmitted (lower)
- מַעֲבֶה: Abbe, 1.25 NA
- דִיאָפרַגמָה: דיאפרגמה של איריס
- מקור אור: Tungsten (incandescent), variable intensity
- סוג תאורה: שדה בהיר
- כּוֹחַ: 110V
- חוֹמֶר: Metal, tungsten
- מידות (H x W x D): 15 x 7.5 x 5.125 אינץ' (בערך)
- מִשׁקָל: 10 פאונד
10. אחריות ותמיכת לקוחות
For warranty information, technical assistance, or to purchase replacement parts, please contact AmScope customer support. Refer to the AmScope webבאתר או במסמכי הרכישה שלך לקבלת פרטי יצירת קשר עדכניים.