dynamic PF-CC-1 proFIRE Purification Column
Product Description
Order Number: PF-CC-1
Table 1. Contents and Storage Information
| Column Specifications | Media Specifications | ||
| Column Volume | 1 mL | Matrix | Hydrophilic porous polymer beads |
| Column material | Polypropylene | Particle size | 30 µm |
| Column size length x I.D. | 26 x 7.0 mm | Functional group | -R-N+(CH3)3 |
| Recommended flow rate | 1 mL/min | pH range | 2 – 12 |
| Maximum flow rate | 4 mL/min | Temp. range | 4 – 60 °C |
| Max. pressure | 0.3 MPa | Shipping solvent | 20 % EtOH aqueous solution |
Handling
Storage and Lifetime
Store in 20 % ethanol at 4 – 35 °C.
For longer lifetime, flush weekly the column with water, then with 20 % EtOH solution. Make sure to close the end tightly to avoid drying out.
Equilibration and Elution
Protein-DNA conjugates are electrostatically bound to the matrix of the column with proFIRE® Buffer A as a first mobile phase, then eluted with a salt-concentration gradient method (proFIRE® Buffer B).
Water-soluble organic solvent (maximum of 30 %) can be added in the mobile phase. Before adding such solvent, make sure that the salt will not precipitate.
Cleaning / Troubleshooting
If the column is showing a change in retention time of the free DNA peak or alterations in peak shape and/or pressure increase, follow the washing steps described below.
- Place buffer tubing A and B in a bottle with water (filtered) and start a “Clean & Sleep” run via Mobile Control software. Leave the column in.
- Place the buffer tubing B in a bottle with 0.1 M to 0.5 M NaOH (filtered) and leave the buffer tubing A in water (filtered).
- Reverse the column and connect the column outlet to the inlet-tubing coming from the pump. Do not connect the other outlet of the column to the tubing going to the flow cell.
- Place the opened outlet of the column in a clean bottle to collect the washing solution.
NOTE: It should be avoided contaminating the system with the washing solution. - Tap the “System Overview” symbol, then tap on “Sample injection” in Mobile control software.
a. Check if the fraction valve port is set to position 13 (waste position)
b. Check if the injection valve port is set to position 1 (bypass position)
c. Select the pump on the left bottom side
d. Set the pump parameters for channel B: Select channel B (100%), adjust the flow to 1 mL/min, tap “Apply” and “Run”. - Collect the fluid eluting from the column and leave the flow for 10 – 15 minutes.
- Set the pump parameters for channel A: Select channel A (100%), adjust the flow to 1 mL/min, tap “Apply” and “Run”.
- Collect the fluid eluting from the column and leave the flow for 15 minutes.
- Stop the flow and reverse the column in its original intended position with both PEEK-tubings connected to the system.
- Put both buffer tubing A and B in a bottle with water (filtered) and start two “Clean & Sleep” runs, one after each other via Mobile Control software.
- Place the tubing A in (filtered and degassed) proFIRE® Buffer A [1] and the tubing B in (filtered and degassed) proFIRE® Buffer B [2] and start a buffer run.
Type in the DNA length 1, inject 160 µL of proFIRE® Buffer A and press “Start”. - Start a DNA only run with a known DNA oligo you can refer to.
Useful Order Numbers
Table 2. Order Numbers
| Product Name | Amount | Order No |
| 10x Buffer A [1] | 50 mL (yielding 500 mL) | PF-BU-A-10 |
| 5x Buffer B [2] | 50 mL (yielding 250 mL) | PF-BU-B-5 |
| proFIRE® Amine Coupling Kit 1 for proteins (>5 kDa); | 5 conjugations | PF-NH2-1 |
| proFIRE® Thiol Coupling Kit 1 for proteins (>5 kDa); | 5 conjugations | PF-SH-1 |
Contact
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
Documents / Resources
 | dynamic PF-CC-1 proFIRE Purification Column [pdf] Instruction Manual PF-CC-1 proFIRE Purification Column, PF-CC-1, proFIRE Purification Column, Purification Column, Column |
