1. Introduction
This user manual provides detailed instructions for the proper use of the Prime Screen Alcohol ETG Urine Test Kit. This kit is designed for the rapid detection of Ethyl Glucuronide (EtG) in human urine at a cutoff concentration of 300 ng/mL. EtG is a direct metabolite of alcohol, indicating recent alcohol consumption. The test provides results within 5 minutes and can detect alcohol presence up to 80 hours after consumption with over 99% accuracy.
This device is intended for in vitro diagnostic use only. It is not intended for medical diagnosis or treatment. Consult a healthcare professional for interpretation of results in a clinical context.
Image 1.1: Prime Screen Alcohol ETG Urine Test Kit, showing the product box and an individual test dip card.
Image 1.2: Illustrates that alcohol can be detected in the body as early as one hour after consumption, providing context for the test's utility.
Image 1.3: Examples of various usage scenarios for the EtG test, including sporting events, breastfeeding, traffic accident investigations, and workplace testing.
2. Important Safety Information
- For in vitro diagnostic use only. Do not ingest.
- Do not use the test kit beyond its expiration date printed on the pouch.
- Store tests in their original sealed pouches until ready for use.
- Do not use if the foil pouch is damaged or opened.
- Handle all urine samples as potentially infectious. Wear protective gloves.
- Keep out of reach of children.
- A positive result does not indicate the level of intoxication or the amount of alcohol consumed.
- A negative result does not necessarily mean the individual has not consumed alcohol, as EtG may be below the detection limit or outside the detection window.
3. Kit Components
Each Prime Screen Alcohol ETG Urine Test Kit contains:
- Individually sealed foil pouches, each containing one ETG urine test dip card and a desiccant.
- This instruction manual.
Materials required but not provided:
- Urine collection container (clean, dry).
- Timer or stopwatch.
- Protective gloves.
4. Setup and Preparation
Before performing the test, ensure the following conditions are met:
- Allow the test device and urine sample to reach room temperature (15-30°C or 59-86°F) prior to testing.
- Do not open the foil pouch until you are ready to perform the test.
- Ensure you have a clean, dry surface to place the test device on after dipping.
5. Operating Instructions
5.1. Urine Sample Collection
Collect a urine sample in a clean, dry container. The sample can be collected at any time of day. For best results, use a fresh urine sample. If testing cannot be performed immediately, the urine sample may be stored at 2-8°C (36-46°F) for up to 48 hours, or frozen at -20°C (-4°F) for longer periods. Bring frozen samples to room temperature and mix thoroughly before testing.
5.2. Performing the Test
- Remove the test dip card from its sealed foil pouch by tearing along the notch.
- Hold the dip card by the end opposite the absorbent strips.
- Dip the absorbent end of the test strip into the urine sample for 10 seconds. Ensure the urine level does not exceed the MAX line on the strip.
- Remove the dip card from the urine and immediately recap the device.
- Lay the recapped test device flat on a clean, dry, non-absorbent surface.
- Start the timer. Read the results at 5 minutes. Do not interpret results after 30 minutes, as this may lead to inaccurate readings.
Image 5.1: Visual guide for performing the test, illustrating dipping the strip, waiting 5 minutes, and reading the result.
6. Interpreting Results
Interpret the results at the 5-minute mark. Do not interpret results after 30 minutes.
6.1. Negative Result
Two lines appear. One colored line appears in the Control region (C), and another colored line appears in the Test region (T). The intensity of the line in the Test region (T) may vary, but any visible line, no matter how faint, indicates a negative result. This means the EtG concentration in the urine is below the 300 ng/mL cutoff level.
6.2. Preliminary Positive Result
One colored line appears in the Control region (C), and NO line appears in the Test region (T). This indicates a preliminary positive result, meaning the EtG concentration in the urine is at or above the 300 ng/mL cutoff level. A preliminary positive result should be confirmed by a laboratory using a more specific method, such as GC/MS.
6.3. Invalid Result
No colored line appears in the Control region (C), or a line appears only in the Test region (T) but not in the Control region (C). An invalid result indicates insufficient sample volume or incorrect procedural techniques. Review the operating instructions and repeat the test with a new test device.
Image 6.1: Visual guide for interpreting test results, showing examples of preliminary positive, invalid, and negative outcomes.
7. Maintenance and Storage
- Store the test kit at room temperature (2-30°C or 36-86°F) in its original sealed foil pouches.
- Do not store in direct sunlight or in areas with high humidity.
- Do not freeze the test devices.
- Ensure the desiccant packet remains in the foil pouch until use to maintain product integrity.
8. Troubleshooting
| Problem | Possible Cause | Solution |
|---|---|---|
| No control line (C) appears. | Insufficient sample volume, incorrect procedure, or expired/damaged test. | Ensure proper dipping technique and sufficient sample. Check expiration date. Repeat test with a new device. |
| Faint line in the Test region (T) for a negative result. | EtG concentration is close to the cutoff level but still below it. | Any visible line in the Test region (T), no matter how faint, indicates a negative result. No action needed unless further confirmation is desired. |
| Results are unclear or difficult to read. | Read time exceeded 30 minutes, or test was not laid flat. | Always read results at 5 minutes. Ensure the test device is placed on a flat, stable surface during development. Repeat test if necessary. |
9. Product Specifications
- Product Name: Prime Screen Alcohol ETG Urine Test Kit
- Model Number: WETG-114
- Detection Target: Ethyl Glucuronide (EtG)
- Cutoff Level: 300 ng/mL
- Detection Window: Up to 80 hours after alcohol consumption
- Result Time: 5 minutes
- Accuracy: Over 99%
- Storage Temperature: 2-30°C (36-86°F)
- Product Dimensions: 4.72 x 2.56 x 4.72 inches (packaging); 3.53 ounces (weight)
- Manufacturer: Wondfo
10. Warranty and Support
For information regarding product warranty, returns, or technical support, please refer to the contact information provided on the product packaging or visit the official Prime Screen website. Ensure to have your product model number (WETG-114) and purchase details available when contacting support.
For general inquiries or to explore other products, you may visit the Prime Screen Store on Amazon.